Enamel Color Change Research Articles

Evaluation of the effects of whitening mouth rinses combined with conventional tooth bleaching treatments.

ObjectivesThe aim of the present study was to evaluate the effect of whitening mouth rinses alone and in combination with conventional whitening treatments on color, microhardness, and surface roughness changes in enamel specimens.Materials and MethodsA total of 108 enamel specimens were collected from human third molars and divided into 9 groups (n = 12): 38% hydrogen peroxide (HP), 10% carbamide peroxide (CP), 38% HP + Listerine Whitening (LW), 10% CP + LW, 38% HP + Colgate Plax Whitening (CPW), 10% CP + CPW, LW, CPW, and the control group (CG). The initial color of the specimens was measured, followed by microhardness and roughness tests. Next, the samples were bleached, and their color, microhardness, and roughness were assessed. Data were analyzed through 2-way analysis of variance (ANOVA; microhardness and roughness) and 1-way ANOVA (color change), followed by the Tukey post hoc test. The Dunnett test was used to compare the roughness and microhardness data of the CG to those of the treated groups.ResultsStatistically significant color change was observed in all groups compared to the CG. All groups, except the LW group, showed statistically significant decreases in microhardness. Roughness showed a statistically significant increase after the treatments, except for the 38% HP group.ConclusionsWhitening mouth rinses led to a whitening effect when they were used after conventional treatments; however, this process caused major changes on the surface of the enamel specimens.

Color change of the bleached enamel treated with calcium silicate- sodium phosphate-sodium monofluorophosphate-based system.

BackgroundThe increasing demand of tooth bleaching has also increased the need of researches focusing on the durability of the resultant color. The aim of this study was to evaluate the effect of a system based on calcium silicate, sodium phosphate and sodium monofluorophosphate (Regenerate™) on color maintenance of bleached enamel considering two waiting times for the contact with a cola drink.Material and MethodsThis in vitrostudy was performed on bovine enamel specimens (n = 100), bleached with 35% H2O2 and treated with: G1 Distilled water; G2 Artificial saliva; G3 RegenerateTM Serum and Toothpaste; G4 RegenerateTM Toothpaste; G5 RegenerateTM Serum. The groups G3, G4 and G5 received one application of the respective products for 3 min, in 3 consecutive days. The color parameters (ΔE, L*, a*, b*) were evaluated by spectrophotometry before and after bleaching and after surface treatments and immersion (15min) in cola drink, on the waiting times of 24 hours (T1) and 7 days (T2). The effect of surface treatments and waiting times was evaluated by Two-Way ANOVA, Kruskal-Wallis, Wilcoxon and T test (p<0.05).ResultsThere was no significant effect of the surface treatments (p=0.57), waiting times (p=0.97) and their interaction (p=0.47) considering ΔE. The analysis of repeated measures of the color coordinates L*, a* and b* showed a decrease of chromaticity (a*, b*) for G3, G4 and G5 and an increase of lightness (L*) for G5, after immersion in cola drink, suggesting some protection against bleached enamel pigmentation.ConclusionsRegenerateTM has a potential protective effect on bleached enamel color maintenance. The waiting times of 24 hours and 7 days for the contact with the cola drink did not influence bleached enamel color maintenance. Key words:Tooth bleaching, hydrogen peroxide, spectrophotometry, pigmentation.

Spectrophotometric Analysis of Color Changes in Enamel Following Exposure to Herbal Bleaching Agents: An In Vitro Study

Spectrophotometric Analysis of Color Changes in Enamel Following Exposure to Herbal Bleaching Agents: An <i>In Vitro</i> Study

Effect of cinnamon extract solution on tooth enamel color

Cinnamon extract solutions can be used as alternatives to standard mouthwash. Indeed, various compounds present in cinnamon, including cinnamaldehyde, eugenol, and tannin, may have antibacterial effects. However, tannin can cause extrinsic tooth discoloration. Therefore, the aim of this study was to identify the effect of cinnamon extract solutions on tooth enamel color. To this end, 12 premolar teeth were immersed in a 4% or 12% cinnamon extract solution (each n = 6) for 60 min, 120 min, and 180 min. Then, the enamel color was evaluated using Vita Easy Shade, and the results were tested with repeated ANOVA and Friedman tests, with post-hoc Wilcoxon tests, to compare enamel color changes between the exposure time groups. Unpaired t-tests and Mann Whitney tests were, then, used to compare enamel color changes between the concentration groups. Greater changes were observed with longer times of immersion and greater cinnamon extract concentration. Regardless, all the samples demonstrated clinically unacceptable color changes (ΔE > 3.5). In addition, the assessment of exposure time groups showed significant changes (p < 0.05), while no significant changes were observed between the concentration groups (p > 0.05). In summary, while the antibacterial compounds found in cinnamon may have benefits for oral health, our findings indicate that cinnamon extract solutions cause clinically unacceptable color changes to tooth enamel.

Effect of betel leaf extract gel on color change in the dental enamel

Betel leaf extract gel contains tannin, a chromogenic agent that causes extrinsic discoloration of the tooth enamel. This study aimed to determine the effect of betel leaf extract gel on color changes in the tooth enamel. Eighteen extracted premolar teeth were divided into three groups (n = 6 each) based on the concentration of the betel leaf extract gel: 15%, 25%, and 35%. Color measurements were performed before application, and after 5, 15, and 26 applications (equivalent to 1, 3, and 6 months of application, respectively). The discolorations were calculated using the CIE L*a*b* formula. Statistical analysis revealed significant changes in color on the enamel surface among the 5, 15, and 26 gel applications at all three concentrations. Thus, duration of application of the betel leaf extract gel has a significant effect on color change of tooth enamel.

Long-term follow-up of enamel color changes after treatment with fixed orthodontic appliances.

The aim of this study was the long-term follow-up of enamel color changes observed in the middle third of buccal tooth surfaces after treatment with fixed orthodontic appliances. The study included 120 maxillary central and lateral incisors and canines of 20 subjects who had fixed orthodontic treatment. The Spectro Shade Micro device (MHT, Verona, Italy) was used toevaluate the color changes of the teeth. Measurements were made from the middle third of the buccal surfaces of the teeth after fixed orthodontic treatment and in month 3, month 6, and year 1 of the retention phase. The Commission Internationale de l'Echairage L* a* b* system that expresses the color coordinates in L*, a*, and b* symbols was used to determine the tooth color, and ΔEvalues between the time periods were calculated. Repeated measurement analysis of variance was used in evaluating the color changes. The increases in ΔL values at 3months, 6months, and 1year after treatment were statistically significant, whereas they were not statistically significant from months 3 to 6, month 3 to year 1, or month 6 to year 1. The decrease in Δa and the increase in Δb values were not statistically significant. ΔE values at all time periods were statistically significant within themselves, and the greatest change was observed 1year after treatment. ΔE values were 1.52 to 3.57, and a visible but clinically acceptable color change occurred. In the first 3months, there was a significant increase in the lightness of the tooth color.

EFFECT OF VARIOUS BLEACHING METHODS ON COLOR CHANGE AND SURFACE ROUGHNESS OF HUMAN ENAMEL

Purpose: This study investigated the effect of different bleaching methods on color change and surface roughness of human enamel. Materials M GP1: 3D Crest White Strips, GP2: Phillips Zoom Whitening Pen, GP3: Rembrandt Deeply White + Peroxide Mouthwash, GP4: Opalescence PF 15% CP, GP5: Opalescence Boost 40% HP (as control group). Bleaching was performed according to manufacturers’ instructions. Color change and enamel surface roughness measurements were performed by spectrophotometer and Atomic Force Microscope respectively at pre and post-bleaching intervals. Data were collected and statistically analyzed using ANOVA followed by Tukey’s HSD Post Hoc test (p≤0.05). Results: For color changes; mean ΔL* values were: 3.92(0.23), 2.44(0.15), 2.5(0.2), 2.6(0.17) and 4.1(0.25) for GP1, GP2, GP3, GP4, and GP5 respectively, Δa* means were: 0.6(0.04), 0.1(0.01), 0.15(0.01), 0.2(0.03) and 0.79(0.05) for GP1, GP2, GP3, GP4, and GP5 respectively, while means for Δb* were: 1.18(0.03), 0.33(0.01), 0.52(0.02), 0.54(0.01) and 1.27(0.07) for GP1, GP2, GP3, GP4, and GP5 respectively. The color change values (ΔE) were: 4.13, 2.46, 2.55, 2.66 and 4.36 for GP1, GP2, GP3, GP4, and GP5 respectively. A statistical significant difference (p≤0.05) is present between Gp1 versus Gp2, Gp3 and Gp4, also between Gp5 versus Gp2, Gp3 and Gp4. No statistical significant difference (p≤0.05) between Gp1 & Gp5 and also between Gp2, Gp3 and Gp4. For surface roughness; mean (Ra) values for pre-bleaching tested groups were: 106.3(8.5), 109.1(10.2), 108(12.2), 110(11.9) and 107(9.7) for GP1, GP2, GP3, GP4, and GP5 respectively. While, post-bleaching values were: 90.3(6.3), 92.3(5.6), 93.6(5.4), 94.7(6.1) and 93.1(3.6) for GP1, GP2, GP3, GP4, and GP5 respectively. However, none of the tested methods showed statistically significant changes in surface roughness (p≤0.05). Conclusions: In-office bleaching may still achieve best whitening effect compared to whitening pen, whitening mouthwash, and home bleaching, while Whitestrips could have a comparable effect. All tested bleaching methods had no effect on enamel surface roughness

Evaluation of surface roughness and color change of bovine enamel after immersion in dye solution

Aim: The aim of this in vitro study was to evaluate the influence of dye solution on enamel color change after bleaching protocols and the effectiveness in maintaining the color of these agents.Material and Methods: The buccal surfaces of sixty-five bovine incisors were cleaned and polished, and the enamel specimens were divided into thirteen groups: G1 to G6: treated with 6% hydrogen peroxide using different surface agents; G7 to G12: treated with 15% hydrogen peroxide using different surface agents; G13: control. After 24 hours, the groups treated were immersed in black tea solution; the control group was stored in artificial saliva. The color was evaluated prior to bleaching, 24 hours later and after immersion in the dye solution; the roughness was measured immediately after bleaching, 24 hours later and 7 days after immersion in the dye solution. The data was analyzed using the Kruskal-Wallis test, followed by the Miller test for roughness analysis, and the Duncan test for color change analysis. It was used 5% significant level with p&lt;0.05.Results: The data found in the evaluation of surface roughness after bleaching indicated a reduction of roughness in all the groups. The surface agent Bifluoride, when applied, showed an increase in roughness after its application and it decreases after immersion in dye solution; the surface agent Desensibilize and the XP Bond adhesive showed greater color alteration after immersion in dye solution.Conclusions: All the groups studied, under different whitening technique, were effective in promoting whitening.

Impact of 35% Hydrogen Peroxide on Color and Translucency Changes in Enamel and Dentin.

This study evaluated the effects of tooth bleaching with high-concentration of hydrogen peroxide on alterations of translucency parameter (TP) and color of dentin and enamel. The crown of five human molars was sectioned into four slices parallel to buccal surface. The dentin of external slices containing buccal/ lingual enamel was fully removed with diamond bur; while these slices were used to assess alterations on enamel. Alterations on dentin were assessed into the center of internal slices. The color of specimens was measured over white and black backgrounds using a spectrophotometer (CieL*a*b) at baseline, allowing to calculate the TP by difference between the color measured over each background. Specimens were submitted to three 15-min applications of 35% hydrogen peroxide followed by their storage in water for one-week. Afterwards, the color measurements were repeated at both backgrounds. Color (∆L, ∆a, ∆b and ∆E) and translucency (∆TP) changes were calculated and data individually analyzed by T-test (α = 0.05). Influence of hard tissue and assessment time on each color parameter was also analyzed by 2-way repeated measure ANOVA (α = 0.05). Tooth bleaching resulted in increased lightness for the enamel, whereas no alteration on this parameter occurred for dentin. No difference between the tooth hard tissues was observed regards the other color parameters and ∆E. A slightly reduction on TP was observed only for the enamel. In conclusion, 35% hydrogen peroxide caused similar color and translucency changes on dentin and enamel.

Effects of repeated bracket bonding on enamel color changes.

The objective of the study is to evaluate the effects of repeated bracket bonding on the color changes of tooth enamel after in vitro coloration. Eighty-one premolars were equally divided into three groups. Samples in Group 1 (G1) represented nonorthodontic treatment patients, and the enamel surfaces were left intact. Samples in Group 2 (G2) and Group 3 (G3) represented orthodontic treatment patients with no repeated bonding and brackets bonded 3 times, respectively. After the brackets were bonded in G2 and G3, samples in all groups were kept in four different staining solutions for 96 h and received 24 h of photoaging. This cycle was repeated 3 times. Brackets were debonded and rebonded in G3 samples after each cycle, whereas brackets were only debonded once in G2 samples after the third cycle. The color changes were assessed using a spectrophotometer at baseline (T1) and after removing the brackets and cleaning the enamel surface (T2). Data (ΔE) were analyzed statistically with analysis of variance between groups, and with Paired t-test within groups. Although the color changes for G1, G2, and G3 were significant (P < 0.05) within groups; the difference was similar between groups (P > 0.05). Repeated bracket bonding does not have a negative influence on the enamel color change after in vitro coloration when compared with intact enamel surface and single bonding.

The effects of utilizing conventional etching versus self etch primer on the enamel color change after treatment with fixed orthodontic appliances: in vivo study

Objectives: The present study was conducted to evaluate clinically the effect of conventional etching and the self etchprimer on the change in enamel color before and after orthodontic treatment. Materialand Methods: The study was conducted on 24 patients treated with fixed orthodonticappliances. Metal brackets were bonded to the teeth mesial to first molars. The bracketswere bonded utilizing Transbond XT adhesiveafter application of either conventional etching (%37 phosphoric acid etch and Transbond XT primer) or Transbond XT self etch primer (splitmouth design). Teeth which showed bracket bond failure were excluded from the study. Therefore, the final number of teeth which were included in the conventional etch and self etch groups were 164 and 160 respectively. The color of the labial and buccal surfaces of the teeth mesial to the first molars was evaluated before bracket bonding and after orthodontic treatment. This step was done with the aid of spectrophotometer Vita Easyshade (Vita Zahnfabri, Germany). The evaluation of the color depended on the color space system of CIELAB (Commission Internationale de l›Eclairage L*, a* and b*). Three parameters were measured (L*, a*, b*). Also, color change(ΔE) was calculated. Then the collected data was statistically analyzed using Paired t-test.Results: All color measurements (L*, a*, b*) were significantly decreased after orthodontic treatment(P<0.05). Conventional etching provided significantly higher ΔE value than the self etch primer (P<0.05).Conclusion: The utilization of conventional etching or self etch primer have a significant negative effect ontooth color after orthodontic treatment. However, the conventional etching has higher pronounced effects.

Effect of Various Bleaching Methods on Color Change and Surface Roughness of Human Enamel

Effect of Various Bleaching Methods on Color Change and Surface Roughness of Human Enamel

Influence of enamel/dentin thickness on the toxic and esthetic effects of experimental in-office bleaching protocols.

This paper aims to assess the whitening effectiveness and toxicity of tooth-bleaching protocols applied to enamel/dentin disks simulating mandibular incisors (ICs) and premolars (PMs). A 10% hydrogen peroxide (H2O2) gel was applied for 3×15, 1×15, or 1×5min to enamel/dentin disks simulating mandibular ICs and PMs, and the trans-enamel and trans-dentinal diffusion products were applied to human dental pulp cells (1h). Professional therapy (35% H2O2-3×15min) was used as positive control, and non-bleached samples were used as negative control. Cell viability and morphology, oxidative stress generation, and odontoblastic marker expression were assessed. The H2O2 diffusion and enamel color change (ΔE) were also analyzed. The 10% H2O2 gel induced significant cell viability reduction only when applied 3×15min, with the intensity of oxidative stress and down-regulation of odontoblastic markers being higher in the IC group. The other experimental bleaching protocols caused slight alterations regarding the cell parameters evaluated, with intensity being related to enamel/dentin thickness. These effects were also correlated with higher H2O2 diffusion in the IC group. ΔE values similar as positive control were found for the 10% 3×15 and 1×15 protocols on IC group, after 4 and 6 sessions. Application of a 10% H2O2 bleaching gel for 15 or 45min to thin dental substrate significantly minimizes cell toxicity in comparison with highly concentrated gels associated with similar esthetic outcomes by increasing the number of bleaching sessions. Bleaching gels with 10% H2O2 applied in small teeth for short periods may be an interesting alternative to obtain whitening effectiveness without causing toxicity to pulp cells, which may be able to reduce the tooth hypersensitivity claimed by patients.

Enamel color changes following orthodontic treatment.

To evaluate and compare the effect of various orthodontic bonding systems and clean up procedures on quantitative enamel colour change. A literature search was done to identify the studies that assessed the quantitative enamel colour change associated with the various bonding systems and cleanup procedures. Electronic database (Pub Med, Cochrane and Google Scholar) were searched. First stage screening was performed and the abstracts were selected according to the initial selection criteria. Full text articles were retrieved and analyzed during second stage screening. The bibliographies were reviewed to identify additional relevant studies. Sixteen full text articles were retrieved. Six were rejected because the methodology was different. There was significant enamel colour change following orthodontic bonding, debonding and clean up procedures. Self-etching primers produce less enamel colour change compared to conventional etching. Resin Modified GIC produces least colour change compared to other light cure and chemical cure systems. Polishing following the clean-up procedure reduces the colour change of the enamel.

Measuring color change of tooth enamel by in vitro remineralization of white spot lesion.

Introduction: Objective colour determination is based on calculating the colorimetric distance (ΔE) within a colour space. So far, the most used colour space in dentistry is CIE L*a*b (Comission Internationale de l´Eclairage). CIE L*C*h* has been recently developed, showing a better correlation with the perception of the human eye. Objective: To determine the ability of an in vitro remineralisation substance to mimic the colour of white spot lesions (WSL) with sound enamel, determining ΔE by using the CIE L*C*h* colour space. Methods: In vitro WSL was generated by immersing 10 samples obtained from human third molars in a demineralization solution for 72 h. Amorphous calcium phosphate stabilized by casein phosphopeptide (CPP-ACP) was then applied for 60 days while maintaining the samples in artificial saliva at 37o C. To evaluate the colour of enamel, images were taken from the samples placed in specifically designed silicone moulds after generating the WSL (pre stage) and after remineralisation by scanning, applying the colorimetric distance equation (ΔE*CMC) according to the Color Measurement Committee. Results: Treatment with CPP-ACP caused a significant ΔE decrease with respect to the pre stage (p <0.001), while the analysis of parameters that make up the colour showed a reduction in the difference of hue (∆H) (p< 0,001) and brightness (∆L) (p< 0,01) after applying CPP-ACP. Discussion: CPP-ACP penetrated to the depth of the white spot lesion, making its appearance similar to that of the sound enamel, probably because of the formation of different mineral phases than that of the original structure, although pores were not completely filled.

Enamel Surface Changes After Exposure to Bleaching Gels Containing Carbamide Peroxide or Hydrogen Peroxide.

This study evaluated the differences in enamel color change, surface hardness, elastic modulus, and surface roughness between treatments with four bleaching gels containing carbamide peroxide (two at 10% and one each at 35%, and 45%) and two bleaching gels containing hydrogen peroxide (two at 40%). Enamel specimens were bleached and color changes were measured. Color change was calculated using either ΔE or the Bleaching Index (BI). Then, surface hardness, elastic modulus, and surface roughness of the enamel specimens were evaluated. All measurements were performed at baseline and directly after the first bleaching treatment for all carbamide peroxide- and hydrogen peroxide-containing bleaching gels. In addition, final measurements were made 24 hours after each of a total of 10 bleaching treatments for carbamide peroxide bleaching gels, and 1 week after each of a total of three bleaching treatments for hydrogen peroxide bleaching gels. After the last bleaching treatment, respective ΔE scores were 17.6 and 8.2 for the two 10% carbamide peroxide gels, 12.9 and 5.6 for the 45% and 35% carbamide peroxide gels, and 9.6 and 13.9 for the two 40% hydrogen peroxide gels. The respective BI scores were -2.0 and -2.0 for the two 10% carbamide peroxide gels, -3.5 and -1.5 for the 45% and 35% carbamide peroxide gels, and -2.0 and -3.0 for the two 40% hydrogen peroxide gels. Each bleaching gel treatment resulted in significant whitening; however, no significant difference was found among the gels after the last bleaching. Whitening occurred within the first bleaching treatments and did not increase significantly during the remaining treatments. Surface hardness significantly decreased after the last bleaching treatment, when 10% carbamide peroxide was used. Furthermore, significant changes in the elastic modulus or surface roughness occurred only after treatment with 10% carbamide peroxide. All six bleaching gels effectively bleached the enamel specimens independent of their concentration of peroxide. Gels with low peroxide concentration and longer contact time negatively affected the enamel surface.

Analysis of the Color and Fluorescence Alterations of Enamel and Dentin Treated With Hydrogen Peroxide

The aim of this study was to evaluate the effect of hydrogen peroxide whitening on fluorescence and color of bovine enamel and dentin. Twenty five dentin discs and 25 enamel discs, with 6 mm diameter and 1 mm thick, were obtained. Direct fluorescence (spectrofluorophotometry) and color (spectrophotometry) were assessed. After fluorescence and color baseline measurements, specimens were immersed in a 35% hydrogen peroxide (HP) solution for 1 h. This procedure was repeated after 7 days. Final fluorescence and color measurements were performed after the second immersion. Chemical characterization of 5 additional specimens was also performed. Data were submitted to repeated analysis of variance and Tukey's test for fluorescence and unpaired t-test for color and chemical components (p<0.05). Fluorescence decreased significantly in dentin specimens after whitening. Enamel presented lower fluorescence than dentin at baseline, but this parameter did not decrease after whitening. Color changes were observed for both substrates, with significantly greater whitening effect in dentin (ΔE=10.37) (p<0.001). Whitening by hydrogen peroxide induced significant decrease in fluorescence of tooth dentin and promoted significant color changes in dentin and enamel with more accentuated outcomes in dentin.

Color Stability of Enamel following Different Acid Etching and Color Exposure Times.

Background and aims. The aim of this study was to evaluate the effect of different etching times on enamel color stability after immediate versus delayed exposure to colored artificial saliva (CAS). Materials and methods. Human first premolars were divided into five groups of twenty. A colorimeter was used according to the CIE system on the mid-buccal and mid-lingual surfaces to evaluate initial tooth color. Samples in group A remained unetched. In groups B to E, buccal and lingual surfaces were initially etched with phosphoric acid for 15 and 60 seconds, respectively. Then, the samples in groups A and C were immersed in colored artificial saliva (cola+saliva). In group B, the teeth were immersed in simple artificial saliva (AS). Samples in groups D and E were immersed in AS for 24 and 72 hours, respectively before being immersed in colored AS. The teeth were immersed for one month in each solution before color measurement. During the test period, the teeth were retrieved from the staining solution and stored in AS for five minutes. This was repeated 60 times. Color changes of buccal and lingual surfaces were calculated. Kruskal-Wallis and Wilcoxon tests were used for statistical analysis (α ≤0.05).Results. There were no significant differences between the groups in term of ΔE of buccal (P = 0.148) and lingual surfaces (P = 0.73). Conclusion. Extended time of etching did not result in significant enamel color change. Immediate and delayed exposure of etched enamel to staining solutions did not result in clinically detectable tooth color changes.

Effect of Microabrasion on Teeth Color

ABSTRACT Objective: The aim of this study is to evaluate the enamel color changes before and after microabrasion technique, which is treatment of white spot lesion by using the CIE L*a*b* system with spectrophotometer. Materials and Methods: Twenty patients with inactive white spot lesions after fixed orthodontic treatment were selected. Especially, only small size white spot areas on central incisors, excluding middle of teeth were accepted. The contents of the microabrasion mixture was 18% hydrochloric acid and pumice (smaller than 30% of buccal area of tooth). The method was applied 4 times for each tooth. The spectrophotometric data on the labial surfaces of teeth were recorded with Easyshade (Vita, Zahnfabrik, Bad Sackingen, Germany) at the center of incisors before and after microabrasion. In vivo spectrophotometric color evaluation was adopted for all patients, and all measurements were performed in the same clinic room under standardized lighting conditions. Color on the surfaces of individual tee...

Mineral loss and color change of enamel after bleaching and staining solutions combination

Pigments of food and beverages could affect dental bleaching efficacy. The aim of this investigation was to evaluate color change and mineral loss of tooth enamel as well as the influence of staining solutions normally used by adolescent patients undergoing home bleaching. Initial hardness and baseline color were measured on enamel blocks. Specimens were divided into five groups (n=5): G1 (control) specimens were kept in artificial saliva throughout the experiment (3 weeks); G2 enamel was exposed to 10% carbamide peroxide for 6 h daily, and after this period, the teeth were cleaned and stored in artificial saliva until the next bleaching session; and G3, G4, and G5 received the same treatments as G2, but after bleaching, they were stored for 1 h in cola soft drink, melted chocolate, or red wine, respectively. Mineral loss was obtained by the percentage of hardness reduction, and color change was determined by the difference between the data obtained before and after treatments. Data were subjected to analysis of variance and Fisher's test (α=0.05). G3 and G5 showed higher mineral loss (92.96 ± 5.50 and 94.46 ± 1.00, respectively) compared to the other groups (p ≤ 0.05). G5 showed high-color change (9.34 ± 2.90), whereas G1 presented lower color change (2.22 ± 0.44) (p ≤ 0.05). Acidic drinks cause mineral loss of the enamel, which could modify the surface and reduce staining resistance after bleaching.