Abstract
Introduction: Objective colour determination is based on calculating the colorimetric distance (ΔE) within a colour space. So far, the most used colour space in dentistry is CIE L*a*b (Comission Internationale de l´Eclairage). CIE L*C*h* has been recently developed, showing a better correlation with the perception of the human eye. Objective: To determine the ability of an in vitro remineralisation substance to mimic the colour of white spot lesions (WSL) with sound enamel, determining ΔE by using the CIE L*C*h* colour space. Methods: In vitro WSL was generated by immersing 10 samples obtained from human third molars in a demineralization solution for 72 h. Amorphous calcium phosphate stabilized by casein phosphopeptide (CPP-ACP) was then applied for 60 days while maintaining the samples in artificial saliva at 37o C. To evaluate the colour of enamel, images were taken from the samples placed in specifically designed silicone moulds after generating the WSL (pre stage) and after remineralisation by scanning, applying the colorimetric distance equation (ΔE*CMC) according to the Color Measurement Committee. Results: Treatment with CPP-ACP caused a significant ΔE decrease with respect to the pre stage (p <0.001), while the analysis of parameters that make up the colour showed a reduction in the difference of hue (∆H) (p< 0,001) and brightness (∆L) (p< 0,01) after applying CPP-ACP. Discussion: CPP-ACP penetrated to the depth of the white spot lesion, making its appearance similar to that of the sound enamel, probably because of the formation of different mineral phases than that of the original structure, although pores were not completely filled.
Highlights
The white spot lesion (WSL) or incipient carious lesion is the first clinical manifestation of the disease and affects only the tooth enamel
When the detailed analysis of each of the parameters of the colour was performed, it was observed that differences in brightness (∆L) and hue (∆H) between the WSL and the sound enamel decreased in the post-treatment stage
The translucency of the enamel is determined by the refractive index of hydroxyapatite and water that accumulates in intercrystalline spaces[8,9]; in the case of sound enamel these spaces are very small and have a very low water content, so that the overall translucency of the enamel is not affected[10]
Summary
The white spot lesion (WSL) or incipient carious lesion is the first clinical manifestation of the disease and affects only the tooth enamel. It appears clinically as an opaque, whitish area with noticeable loss of translucency visible after drying of the area, or even in the presence of moisture, depending on the progress of the lesión[1]. One of the main aspects taken into account when different remineralising substances are studied is their ability to blend the colour of the affected area with the appearance of the surrounding sound enamel. Measuring color change of tooth enamel by in vitro remineralization of white spot lesion
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