Emulsion Template Method Research Articles

κ‐Carrageenan/poly(sodium styrenesulfonate‐co‐acrylic acid) macroporous anionic monoliths: Dye adsorption and oil–water separation properties

Abstractκ‐Carrageenan (κC) is a widely used food hydrogel, but its use as an environmental adsorption separation material still needs to be further developed. Herein, a novel macroporous anionic monolith was synthesized by the oil‐in‐water high internal phase emulsion template method. The monolith was obtained by the continuous phase polymerization of sodium styrenesulfonate (NaSS) and acrylic acid (AA), in which κC was introduced to form a semi‐interpenetrating network structure to improve its overall performance. The results showed that the mechanical strength of the monolith increased with κC content. Because of the use of two strong hydrophilic monomers, NaSS and AA, the porous monolith proved to be superoleophobic underwater. In addition, the porous materials had a strong cationic dye adsorption capacity (2455 mg/g for malachite green, 1180 mg/g for methylene blue) and a fast removal rate, good reusability, and oil–water separation. This work highlights their potential application value in the purification of complex water environments.

Monolithic Catalysts Supported by Emulsion-Templated Porous Polydivinylbenzene for Continuous Reduction of 4-Nitrophenol.

A monolithic catalyst was fabricated through an emulsion-templating method, postpolymerization modification, and in situ loading of active constituents. To achieve a high specific surface area, divinylbenzene (DVB) was solely employed as the monomer, while the porous structure was adjusted with the porogen content and the types of initiators. Then, anchor points were introduced on the pore wall through nitration and amination of the polymeric scaffold. Using a controlled "silver mirror reaction", monolithic catalysts were obtained after loading of silver nanoparticles (Ag NPs), which was verified from morphological and crystallinity characteristics. The catalytic performance of the resultant monolithic catalyst was determined with the model reduction of 4-nitrophenol (4-NP). In static catalysis, the monolithic catalyst was proved to have a reactively high apparent rate constant and a good reusability. Furthermore, a flow reactor was fabricated with the monolithic catalyst, showing a high efficiency and long-term durability for the continuous reduction of 4-NP. This work broadened the adjustment of porous structures and the subsequent application for emulsion-templated monoliths.

Reversed Yolk–Shell Dielectric Scatterers for Advanced Radiative Cooling

AbstractPassive daytime radiative cooling dissipates heat from surfaces by reflecting sunlight and emitting infrared radiation to the cold outer space, featuring a zero‐energy consumption. Polymer‐dielectric radiative cooling coatings have received significant attention because of their scalable preparation, low cost, and ease of use. However, current dielectric scatterers reveal limited solar reflectance in the polymer‐dielectric coatings due to the optical crowding, which reduces cooling effect and hinders practical application. Employing a scalable emulsion templated method, reversed yolk–shell dielectric scatterers (RYSS) are developed to alleviate optical crowding effects in polymer‐dielectric coatings. This results in coatings with a solar reflectance of ≈97.4% and an infrared emittance of ≈96.9%, while achieving a substantial reduction in scatterers content. Besides, simulation results show that RYSS outperforms core–shell, hollow, and solid scatterers in enhancing solar reflectance. Using phase change materials as emulsion templates, RYSS with high heat storage effectively enhance daytime cooling and reduce nighttime over‐cooling. Moreover, cooling experiments on concentrating photovoltaics demonstrate a temperature drop of 42 °C (≈26.2 times extension of lifetime) and a notable 22% increase in open‐circuit voltage. This work suggests that RYSS presents a promising solution to overcome the challenges of radiative cooling materials and pave the way for their practical applications.

Dual-templating synthesis of superhydrophobic Fe3O4/PSt porous composites for highly efficient oil-water separation

Fe3O4/PSt porous composites with low density, interconnected network structure and superhydrophobic were prepared successfully by a dual-templating method, including High Internal Phase Emulsion (HIPEs) template method and the introduction of organic template species. It was worth mentioning that a novel amphiphilic di-block copolymers P(DMAEMA-b-St) were prepared as emulsifier by reversible addition fragmentation chain transfer (RAFT) polymerization method. At the same time, the effects of PS block length, emulsifier content, Fe3O4 content, DVB content and dispersed phase proportion on HIPEs droplet size and the corresponding polyHIPEs oil absorption were researched. The results showed that the oil adsorption capacity of 9.43 g/g toward toluene under optimal conditions. Subsequently, adsorption isotherm and adsorption kinetics were investigated. The results were shown that the adsorption of oil on adsorbent followed Freundlich model and pseudo-second-order (PSO) model. In addition, the adsorption experiments on a variety of oils demonstrated the wide applicability of Fe3O4/PSt porous composites. The cycling experiments proved that the Fe3O4/PSt porous composites had excellent cycling ability, were easy to recover and could be reused for multiple times. Finally, the adsorption column experiments showed that Fe3O4/PSt porous composites had powerful potential for industrial applications.

Monolithic MAF-6 aerogel with hierarchical pore structure for methanol/ethanol separation and Knoevenagel reaction

The synthesis of monolithic MOF aerogel is a critical step in applying MOFs to many practical applications. MAF-6 (RHO-[Zn(eim)2], Heim = 2-ethylimidazole) is a type of MOF materials with large cavities and apertures. However, it has been much less studied due to the difficulty in obtaining it via conventional synthesis protocols. Herein, a nearly pure MAF-6 monolith with macro/meso/microporous pore structure is synthesized in situ by emulsion template method, which exhibits hierarchical structure with high porosity (∼80 %), large specific surface area (up to 1135 m2/g), and excellent mechanical properties. In addition, the monoliths demonstrate excellent capability and stability to separate methanol/ethanol mixtures and high catalytic performance in the Knoevenagel reaction. This work provides an effective approach to prepare MAF-6 monoliths for potential application in catalysis, adsorption, separation, etc.

Efficient reduction of photopolymerization shrinkage stress through microporous particles templated from Pickering emulsion droplets

Photopolymerization of acrylate based resin is a rapid, efficient and low-energy curing process that is achieving wide applications in many industrial fields. However, the volumetric shrinkage and the shrinkage stress of the cured resin have long been the major factor reduces adhesion strength of UV curable coatings onto the substrate. In this paper, the Pickering emulsion template method is employed to prepare elastic microporous particles with well-defined surface and internal structures and these microporous particles are thus added into the UV curable resin formulation to verify their capability to reduce polymerization stress through the microporous structure. The gel point time of UV-curable materials is delayed by 11 s with 1.5 wt% addition of the microporous particles and the double bond conversion at the gel point is subsequently increased. By real time MIR-photo-rheology and 3D line scanning laser confocal measurement, it is found that addition of microporous particles into the resin formulation can reduce the volume shrinkage of the resin by 61.2 % with only 1.5 wt% microporous particles, while the excellent mechanical and thermal properties of the cured resin is basically maintained.

Preparation of emulsion hydrogels encapsulating extractant by the Pickering emulsion template method to recover lanthanum ions in aqueous solutions

Abstract To solve the problem of liquid–liquid extraction of La(III), the oil-in-water Pickering emulsions were prepared by utilizing the aqueous solution of sodium alginate as the continuous phase, kerosene-diluted extractant di-(2-ethylhexyl) phosphate (P204) as the dispersed phase, and modified silica as an emulsifier. Then the emulsions were added to a calcium chloride solution to prepare the Pickering emulsion hydrogels (PEHGs) to better remove La(III). The PEHGs were characterized using Fourier transform infrared, thermogravimetric analysis, and scanning electron microscopy. The adsorption properties of PEHGs for La(III) in the aqueous solution were investigated using a UV–vis spectrophotometer. The study found that P204 was successfully coated by hydrogels and reached the highest adsorption capacity of 48 mg/g at pH 4. The amount of adsorption increased with the rise in temperature from 298 to 318 K. La(III) adsorption experimental data were more consistent with the pseudo-second-order kinetic model and the Langmuir isotherm model. Thermodynamic parameters showed that the adsorption of La(III) by PEHGs was a spontaneous endothermic process. The internal diffusion model revealed a linear relationship, indicating that internal diffusion played a role in the adsorption process. The encapsulating property of PEHGs indicated its potential usefulness in industrial wastewater for treating La(III).

Amphiphilicity evolution in self-assembled carbon-based nanostructures for stabilizing different types of Pickering emulsions

ABSTRACT Carbon-based nanostructures have been firmly established as highly scalable and versatile platforms, which have found extensive applications in several fields of multifunctional nanocomposites, energy storage, environment protection, and electronics device. Pickering emulsion template method is one of the efficient strategies for assembling metastable carbon nanomaterials into stable macroarchitectures. Here, the combination of graphene oxide (GO) and carbon nanotubes (CNTs) was chemically and continuously treated with 2-ethyl-4-methylimidazole (EMI) to acquire tunable wettability. The hybridization of CNTs and reduction of GO can make the as-synthesized carbon nanohybrids be capable of stabilizing oil-in-water (O/W) and water-in-oil (W/O) Pickering emulsions, respectively. The major shift in the type of Pickering emulsion is conveniently implemented through the catalytic reduction of GO by EMI. The sp2-hybridized carbon networks self-assembled with CNTs and reduced graphene oxide (RGO) are intentionally located at the biphasic liquid interface of different types of Pickering emulsions, which can endow the architecture elaborated from Pickering emulsion strategy with multiple functionalities.

Transparent Oil-Water Separating Hydrophobic Sponge Prepared from a Pickering High Internal Phase Emulsion Stabilized by Octadecyltrichlorosilane Grafting Carbon Nanotubes.

Increasingly, oil spills and industrial discharges are wreaking havoc on the water environment; in order to efficiently separate oil and water from sewage containing oil or organic solvents, a novel porous polymer (P(EHA-co-BA)) was prepared by Pickering high internal phase emulsion (HIPE) template method. To obtain polyHIPE with better oil/water separation capacities, octadecyltrichlorosilane (OTS)-modified carbon nanotubes (CNTs) and surfactants were used as costabilizers for HIPE, which improved the stability of HIPE as well as the mechanical properties and the separation efficiency of polyHIPE. In the presence of 1 wt % OTS-CNT adding in the oil phase, 1%OTS-CNT polyHIPE has high porosity (92.21%), favorable hydrophobicity (a water contact angle of 128°), and excellent mechanical properties. As a result, 1%OTS-CNT polyHIPE has high absorption of oils and oily solvents, e.g., dichloromethane up to 36 g/g, and maintains an absorption efficiency of >97% after 20 reapplications. In the formulation of polyHIPE, cinnamaldehyde (CA) has been added to provide superior antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). It appears that the novel polyHIPE proposed in this work is a reusable antibacterial porous polymer with promising applications for oil-water separation.

Emulsion template fabricated gelatin-based scaffold functionalized by dialdehyde starch complex with antibacterial antioxidant properties for accelerated wound healing

Gelatin and starch are considered as promising sustainable materials for their abundant production and good biodegradability. Efforts have been made to explore their medical application. Herein, scaffolds based on gelatin and starch with a preferred microstructure and antibacterial antioxidant property were fabricated by the emulsion template method. The dialdehyde starch was firstly combined with silver nanoparticles and curcumin to carry out the efficient hybrid antibacterial agent. Then, the gelatin microsphere of appropriate size was prepared by emulsification and gathered by the above agent to obtain gelatin-based scaffolds. The prepared scaffolds showed porous microstructures with high porosity of over 74 % and the preferred pore sizes of ∼65 μm, which is conducive to skin regeneration. Moreover, the scaffolds possessed a good swelling ability of over 640 %, good degradability of over 18 days, excellent blood compatibility, and cell compatibility. The promising antibacterial and antioxidant properties came from the hybrid antibacterial agent were affirmed. As expected, the gelatin-based scaffolds fabricated by the emulsion template method with a preferred microstructure can facilitate more adhered fibroblasts. In summary, gelatin-based scaffolds functionalized by starch-based complex expanded the application of abundant sustainable materials in the biomedical field, especially as antibacterial antioxidant wound dressings.

Tiger Nut Oil-Based Oil Gel: Preparation, Characterization, and Storage Stability.

In this study, Tiger nut (Cyperus esculentus L.) oil-based oleogels were prepared using the emulsion template method with whey protein (WPI; 0.5-2.5% (w/v) and Xanthan gum (XG; 0.1-0.5% (w/v). The microstructure of the oleogels obtained from the high internal phase emulsion (HIPE) and an emulsion after further shearing were observed using an optical microscope and laser confocal microscopy. A series of rheological tests were conducted to evaluate the effect of WPI and XG concentrations on the strength of the emulsion and oleogel. The texture, oil holding capacity, and oxidative stability of oleogels were characterized. The results showed that XG alone could not form oleogel, while the concentration of WPI had more effect than XG. When WPI was at a fixed concentration, the viscoelasticity of HIPE increased with the addition of XG. This was due to the complexation of WPI and XG, forming a stable gel network between the tight emulsion droplets and thus giving it a higher viscoelasticity. With an increase in WPI concentration, the stability and viscoelasticity of the emulsion were increased, and the oil-holding capacity and gel strength of the oleogels were enhanced. Moreover, the addition of XG could significantly enhance the stability and viscoelasticity of the emulsion (p < 0.05), and an increase in the concentration had a positive effect on it. The oleogels showed high gel strength (G' > 15,000 Pa) and good thixotropic recovery when the XG concentration was higher than 0.3% (w/v). WPI (2.0%) and XG (>0.3%) could be used to obtain HIPE with good physicochemical and viscoelastic properties, which in turn lead to oleogels with minimal oil loss, viscoelastic and thixotropic recovery, and temperature stability. Compared with tiger nut oil-based oleogel, tiger nut oil contained more polyunsaturated fatty acids, which were more easily decomposed through oxidation during storage and had lower oxidation stability. This study provides a reference for the preparation of oleogels from food-approved polymers and provides additional theoretical support for their potential application as solid fat substitutes.

Construction of soybean oil bodies–xanthan gum composite oleogels by emulsion-templated method: Preparation, characterization, and stability analysis

Oil bodies (OBs), a special tissue that stores oil in plant cells, are rich in polyunsaturated fatty acid, vitamin E, phytosterols, and other functional components. In this study, different concentrations of xanthan gum (XG)-stabilized OBs were prepared using the electrostatic deposition technique, and oleogels were prepared via freeze-drying and shearing effects. The relationships between the properties of OB emulsions (OBEs), intermediate products (freeze-dried samples), and end products (oleogels) were explored. The XG addition significantly enhanced the stability of OBs. Particularly, when it achieved 1.5%, the average particle size of OBEs was the least at 892.03 nm, the ζ-potential absolute value was greater than 30 mV, and the porosity was a minimum of approximately 3.68%. Freeze-dried samples with XG had less oil on their surfaces, and their hardness and springiness were significantly and positively correlated with XG addition. The excellent stability of the OBEs and freeze-dried samples resulted in the formation of a dense and stable three-dimensional network structure to hold the oil. The oil holding capacity, rheological properties (including higher yield stress, stronger elastic, and thixotropy behavior), and thermal stability of the oleogels improved with increasing XG addition. Moreover, the compact networks and excellent oil holding capacity of the OB-based oleogels with XG effectively hindered high-temperature destruction and oil oxidation. The correlation analysis and the scheme model indicated that the oleogel properties could be traced back to the interaction effects among the OB components. These results provide guidance for the design and quality control of OB-based oleogels.

Polyurethane Acrylate Oligomer (PUA) Microspheres Prepared Using the Pickering Method for Reinforcing the Mechanical and Thermal Properties of 3D Printing Resin

Some photosensitive resins have poor mechanical properties after 3D printing. To overcome these limitations, a polyurethane acrylate oligomer (PUA) microsphere was prepared using the Pickering emulsion template method and ultraviolet (UV) curing technology in this paper. The prepared PUA microspheres were added to PUA-1,6-hexanediol diacrylate (HDDA) photosensitive resin system for digital light processing (DLP) 3D printing technology. The preparation process of PUA microspheres was discussed based on micromorphology, and it was found that the oil-water ratio of the Pickering emulsion and the emulsification speed had a certain effect on the microsphere size. As the oil-water ratio and the emulsification speed increased, the microsphere particle size decreased to a certain extent. Adding a suitable proportion of PUA microspheres to the photosensitive resin can improve the mechanical properties and thermal stability. When the modified photosensitive resin microsphere content was 0.5%, the tensile strength, elongation at break, bending strength, and initial thermal decomposition temperature were increased by 79.14%, 47.26%, 26.69%, and 10.65%, respectively, compared with the unmodified photosensitive resin. This study provides a new way to improve the mechanical properties of photosensitive resin 3D printing. The resin materials studied in this work have potential application value in the fields of ceramic 3D printing and dental temporary replacement materials.

Camellia Saponin Based Oleogels by Emulsion-Templated Method: Preparation, Characterization, and in Vitro Digestion

Camellia Saponin Based Oleogels by Emulsion-Templated Method: Preparation, Characterization, and <i>in Vitro</i> Digestion

Fabrication of amphoteric gelatin nanospheres-doped self-healing nanocomposite hydrogels and the application in flexible sensors

The nanomaterials with two distinct properties or components have outstanding advantages in the synthesis of smart hydrogels. In this work, gelatin nanospheres (GNPs) asymmetrically coated with poly(diallyldimethylammonium chloride) (PDDA) and tannic acid (TA) were synthesized by Pickering emulsion template method. The nanocomposite hydrogel with amphoteric gelatin nanospheres (GPTNPs)-doped was successfully prepared, which has excellent mechanical properties (strain = 616%, stress = 2.85 MPa) and self-healing properties (efficiency = 90.9%) under the synergistic effects of hydrogen bonding and metal coordination. The developed GPTNPs/polyacrylic acid (GPTNPs/PAA) hydrogel sensor exhibits high sensitivity (gauge factor (GF) = 3.69), quick response time (96.4 ms) and superior stability. Therefore, the GPTNPs/PAA hydrogels are excellent materials for application in wearable flexible sensors to monitor real-time human motion.

Flaxseed Gum/Arabic Gum/Tween 80-Based Oleogel as a Fat Substitute Applied in Emulsified Sausage: Physicochemical Properties, Sensory Attributes and Nutritional Quality.

In the present study, flaxseed gum (FG), Arabic gum (GA) and Tween 80 were used to prepare oleogels through an emulsion-templated method, and the obtained oleogels were designed for the partial substitution of pork fat in emulsified sausage. An increment in FG concentrations enhanced the viscoelasticity of emulsions, which resulted in the improved stability of emulsion systems, with smaller droplet sizes. In addition, increased FG concentrations contributed to higher mechanical strength, denser network structure and lower oil loss of oleogels. As a fat substitute, the prepared oleogels improved the textural properties and nutritional quality of emulsified sausages. With the increase in the substitution level of oleogels, the hardness and chewiness of the emulsified sausage increased, and the cooking loss decreased. Meanwhile, the reformulation with oleogels decreased the saturated fat from 57.04 g/100 g lipid to 12.05 g/100 g lipid, while increasing the ratio of omega-6 to omega-3 essential fatty acids from 0.10 to 0.39. The obtained results demonstrated that the flaxseed gum/Arabic gum/Tween 80-based oleogels had huge potential to successfully replace pork fat in emulsified sausage products.

Development by emulsion templating of a novel Tunisian clay-polyvinyl alcohol/extra-virgin olive oil scaffold with antibiofilm properties

A biobased porous scaffold with antibacterial and antioxidant properties was developed using the emulsion templating method. This technique represents a new route for the development of porous materials with controllable pore size. To take into account the environmental and economic aspects, polyvinyl alcohol (PVA) was chosen as the polymer matrix, raw Tunisian clay as the Pickering emulsion stabilizer, and extra virgin olive oil (EVOO) as the oil phase. PVA is often chosen for producing wound dressings that create a moist environment and consequently promote healing. Clay was used as a barrier against bacteria and as emulsifier due to its nanoscale and layered structure. In EVOO, the polyphenolic compounds have antioxidant activity and fatty acids have a plasticizer role. X-ray fluorescence, X-ray diffraction and Fourier transform infrared spectroscopy were used to analyze the raw clay. Then, the obtained films were structurally and chemically characterized, and their performance, antimicrobial activity against Pseudomonas aeruginosa, and antioxidant activity were evaluated. The emulsion characterization showed that clay addition influenced the emulsion type and increased the emulsion stability index to 98%, even after 3 months of storage. This was explained by the irreversible adsorption of clay platelets on the droplet surface that formed a rigid shell to prevent coalescence. The film characterization demonstrated that the oil: water ratio did not influence the surface porosity, although it affected the pore size uniformity and interconnection. Conversely, shortening the curing time improved the surface porosity. The resulting PVA-clay/EVOO scaffold displayed an exceptional performance, including high mechanical strength (∼2.1 MPa), interconnected porous structure with a size ranging from few µm to > 30 µm, and hydrophobic properties (∼90° ± 2). In addition, it showed antiadhesive and antibiofilm activities against P. aeruginosa. Therefore, this scaffold could be used for the early prevention of biofilm formation during wound healing and is a promising candidate for the controlled release of drugs and potentially for various applications in soft tissues.

Investigation of the mechanism of establishing a solid–water–oil system by metastable droplets and their application in the fabrication of polyol nanosheets for rapid boron removal

The emulsion method is a typical way for the preparation of nanomaterials, however, it usually requires a lot of energy and surfactants to form and stabilize of emulsions. A critical factor limiting its application is to control the morphology of the material by modulating the size of the emulsion droplets. In this work, a novel solid-water–oil (S-W-O) system based on the integration of metastable emulsion and salt template method was proposed, and the functionalized silica nanosheets were prepared by the one-pot method. The size of the prepared nanosheets is relatively uniform, with a length of 800 to 1200 nm and a width of about 200 nm. Subsequently, a large number of epoxy group sites were introduced into the nanosheets relying on distillation precipitation polymerization, and the polyol groups were successfully loaded. Finally, the polyol-functionalized nanosheet adsorbent (NS@GMA-NMDG) was obtained and applied for the rapid removal of boron. NS@GMA-NMDG shows favorable adsorption performance for boric acid in solution, with a maximum adsorption capacity of about 35.49 mg g−1 based on the Langmuir model fitting and a fast adsorption rate of about 30 min to reach 96.3% of the adsorption equilibrium concentration. Remarkably, the investigation of the formation mechanism of nanosheets reveals that salt plays an important role in the S-W-O system. This study not only puts forward a novel method of designing and synthesizing nanosheet adsorbents, but also the application of surfactant-free and low-energy emulsification reflects the concepts of energy saving and clean production, which provides fresh inspirations for the development of new environmentally friendly adsorbents.

Stapled anoplin peptide combined with photothermal therapy enhances oncolytic immunotherapy of triple-negative breast cancer

This study constructed a nano-drug delivery system, A3@GMH, by co-delivering the stapled anoplin peptide(Ano-3, A3) with the light-harvesting material graphene oxide(GO), and evaluated its oncolytic immunotherapy effect on triple-negative breast cancer(TNBC). A3@GMH was prepared using an emulsion template method and its physicochemical properties were characterized. The in vivo and in vitro photothermal conversion abilities of A3@GMH were investigated using an infrared thermal imager. The oncoly-tic activity of A3@GMH against TNBC 4T1 cells was evaluated through cell counting kit-8(CCK-8), lactate dehydrogenase(LDH) release, live/dead cell staining, and super-resolution microscopy. The targeting properties of A3@GMH on 4T1 cells were assessed using a high-content imaging system and flow cytometry. In vitro and in vivo studies were conducted to investigate the antitumor mechanism of A3@GMH in combination with photothermal therapy(PTT) through inducing immunogenic cell death(ICD) in 4T1 cells. The results showed that the prepared A3@GMH exhibited distinct mesoporous and coated structures with an average particle size of(308.9±7.5) nm and a surface potential of(-6.79±0.58) mV. The encapsulation efficiency and drug loading of A3 were 23.9%±0.6% and 20.5%±0.5%, respectively. A3@GMH demonstrated excellent photothermal conversion ability and biological safety. A3@GMH actively mediated oncolytic features such as 4T1 cell lysis and LDH release, as well as ICD effects, and showed enhanced in vitro antitumor activity when combined with PTT. In vivo, A3@GMH efficiently induced ICD effects with two rounds of PTT, activated the host&apos;s antitumor immune response, and effectively suppressed tumor growth in 4T1 tumor-bearing mice, achieving an 88.9% tumor inhibition rate with no apparent toxic side effects. This study suggests that the combination of stapled anoplin peptide and PTT significantly enhances the oncolytic immunotherapy for TNBC and provides a basis for the innovative application of anti-tumor peptides derived from TCM in TNBC treatment.

Stretchable porous conductive hydrogel films prepared by emulsion template method as flexible sensors

Conductive hydrogels with stretchability, breathability and long-term stability have attracted increasing attention for the applications in interactive wearable devices, artificial prosthetics, and intelligent robots. In this paper, a stretchable (up to 254 %), well breathable (vapor transmission up to 1.231×10−2 g·cm−2·h−1), and stable conductive (1.21 S/m) hydrogel films (Gel/PPy/Ag) was prepared by gel-template immersion method using gelatin as gel networks, polypyrrole (PPy) and silver (Ag) as conductive reinforcing filler and Span 80 as emulsion template. The film could maintain flexibility and conductivity after stored in an environment of –20 °C or 25 °C for 20 days. The excellent frost resistance and water retention ensured the practical application of the porous organic hydrogel film with environmental adaptability and long-term stability. The strain sensor made from the Gel/PPy/Ag films presented excellent sensitivity (GF reached 2.034), good durability, fast response (0.34 s) and recovery ability (0.36 s). It monitored weak and vigorous human motions effectively and sensitively. The Gel/PPy/Ag flexible electrodes realized the real-time sensitive detection of human Electrocardiogram (ECG) and electromyogram (EMG) signals and precision sensing control of the manipulator arm. The Gel/PPy/Ag hydrogel and the prepared flexible electronic devices have broad application prospects in the field of health monitoring, motion monitoring, operation control and other soft electronic devices.