Predicting the responses and injuries of seated pilots caused by emergency landing or ejection is significant for the design of ejection seats. A nonlinear two-dimensional multi-body human/seat model is proposed to predict the responses and body injuries exposed to vertical impact. Then a new force identification method is presented and applied to obtain the external excitation of the system. In addition, the parameters of this model are optimized based on the Error Assessment of Response Time Histories (EARTH) metric and Non-dominated Sorting Genetic Algorithm II (NSGA-II). Furthermore, utilizing the proposed model, this research predicts body injuries with different impact intensities. With body injuries as output, the influence of input parameters is assessed by global sensitivity analysis (GSA) methods. The result shows that the backrest angle plays a crucial role in body injuries. Finally, the influence of backrest angle is further analyzed to obtain the variation of body injuries with backrest angle. This research provides guiding principles for the subsequent design of ejection seats.