Electrophoretic Pattern Of Plasma Proteins Research Articles

Influence of short-term storage conditions on the stability of total protein concentrations and electrophoretic fractions in plasma samples from loggerhead sea turtles, Caretta caretta

Plasma protein electrophoresis has been proposed for use in reptile medicine to aid in the diagnosis of disease. Reference intervals of plasma proteins in loggerhead sea turtles have been established; however, studies evaluating the effect of storage conditions on plasma protein and protein fractions in this species are lacking. The aim of this study was to evaluate the effect of short-term storage conditions on total protein concentration and electrophoretic fractions of sea turtle plasma. Blood was collected from sea turtles, and plasma obtained after centrifugation was fractioned into five aliquots. One aliquot was analyzed within 3 h after collection, whereas the remaining aliquots were stored at either +4 °C or −20 °C for 2 or 7 days. Overall, there was a significant effect of storage temperature and duration on albumin, β-globulin, and A/G ratio (Friedman rank sum test P < 0.001) but not on total protein concentration. The results of the current study show that short-term storage conditions influence the electrophoretic pattern of plasma proteins and the A/G ratio in Caretta caretta. Therefore, in order to obtain plasma protein concentrations that can serve as reliable screening tests in veterinary diagnostic laboratories, practitioners should refrigerate plasma for no more than 2 days.

Haemolymph of femaleOxya hyla hyla serville (Orttioptera: Acrididae): A preliminary study

The preliminary light microscopic and biochemical study of the haemolymph ofOxya hyla hyla records the presence of five different types of haemocytes and gives a quantiative estimation of plasma proteins along with their gel electrophoretic band patterns. The higher number of total haemocyte count in adult female compared to that of immature one may be attributed to some kind of synthesis and transport of proteins or other yolk materials during vitellogenesis. High plasma protein concentration during monsoon may be associated with the reproductive maturity of these insects. The gel electrophoretic pattern of plasma proteins reveals the constant presence of two particular bands suggesting the occurrence of lipoprotein and juvenile hormone carrier protein.

Electrophoretic properties of plasma protein relating to som blood properties in cultured yellow tail.

The electrophoretic properties of plasma protein of cultured yellow tail (body weight 2.6±0.34kg, n=31) were examined on cellulose acetate and polyacrylamide gel. The ranges and mean values of hematocrit, total plasma protein, and lipid were also measured. 1) Cellulose acetate electrophoresis of the plasma showed mainly 6 protein fractions. Although the electrophoretic patterns and percentage composition of plasma protein were similar to those of young ye'low tail (body weight 286±55.1g, n=10), their lipoprotein patterns were quite different. It was suggested that the physiological function of plasma protein components was not consistent throughout whole the life of the specimen. 2)A wide range in hematocrit values was obtained. However, the correlation coefficient between hematocrit value and amount of plasma protein was 0.75, and that between hematocrit value and amount of plasma lipid was 0.71. 3)No correlation was observed between hematocrit value and the electrophoretic pattern of plasma proteins.

The sensitivity of function tests in detecting liver damage in the rat

The sensitivity of a group of tests in detecting the effects of liver damage was investigated in groups of rats treated with carbon tetrachloride or sodium selenate for 5 months. The tests included determinations for the activity of plasma glucose-6-phosphatase, glutamic-pyruvic transaminase, cholinesterase, bilirubin concentration, protein fractions, colloidal red flocculation, zinc sulfate turbidity, urine coproporphyrin and urobilinogen determination, the sulfobromophthalein (BSP) and hippuric acid tests, relative liver weight and gross and histopathologic examination of the liver. The consequence of carbon tetrachloride administration differed from the effects of sodium selenate in the morphological form of liver injury and in the type of functional impairment (as shown by the BSP and hippuric acid tests). The hippuric acid test, the electrophoretic pattern of plasma proteins, and the BSP test were the most effective tests in revealing long-term impairment of liver function. Histopathologic evaluation and the determination of plasma glutamic-pyruvic transaminase were sensitive parameters in detecting structural abnormality. However, function tests alone did not give reliable information about structural changes in the liver; both types of tests are necessary for a satisfactory assessment of hepatic integrity. Restriction of food intake had no effect on the results of any of these tests.

西九州沿岸域のマアジの血漿蛋白電気泳動像と血液要素について

To analyse the population of the jack mackerel, Trachurus japonicus, a total of 785 individuals, in 25 groups, were collected from 12 localities along the western coast of Kyushu. These specimens were examined for some blood elements and the electrophoretic pattern of plasma proteins. The ecological significance of the results obtained was then discussed from the fisheries point of view.1. The specific gravity of blood, hematocrit value and the amount of plasma proteins were compared between the two groups of blood specimen. The one withdrawn from fish immediately after catch by angling, and the other withdrawn from the fish kept in a concrete tank for 56 days. All the obtained values in the latter group were higher than in the former. While, the electrophoretic patterns of plasma protein of the two groups were quite identical.2. Electrophoretic patterns of plasma protein of the sepcimens kept in floating fish cages at several places in the northern part of Nagasaki Pref. were all the same. On the other hand, four different types were found in the fish collected from Goto-nada and the coast of Kagoshima Pref.3. Although the correlation coefficient between the hematocrit value and the specific gravity of blood was as high as +0.92, the coefficient between the specific gravity of blood and the amount of plasma protein was +0.51, and that between the hematocrit value and the amount of plasma protein was +0.53.

Changes in blood components of the mullet, Mugil cephalus L., following treatment with salmon gonadotropin and methyltestosterone

1. 1. Blood from twenty-six stripped mullet ( Mugil cephalus) injected with salmon gonadotropin and methyltestosterone was compared to blood from saline injected controls for changes in hematocrit, total dissolved plasma solids, plasma osmolarity, total plasma calcium and copper, and electrophoretic patterns of plasma proteins. 2. 2. In all hormone treated fish, hematocrits increased slightly, plasma solids increased significantly ( P ⩽ 0.05) and osmolarity was not affected. 3. 3. Total plasma calcium increased with the higher (5.0 mg) gonadotropin dose, and increased markedly in methyltestosterone treated fish. Plasma copper changes followed those of calcium but were not pronounced. 4. 4. Methyltestosterone, in contrast to salmon gonadotropin, induced distinct qualitative and quantitative changes in plasma protein electropherograms.

Blood Protein Variation in the Bufo americanus Species Group of Toads

electrophoretic patterns of plasma proteins of the Bufo americanus species group of toads. This variation was sufficient to negate any possible calculation of affinities between the B. americanus and other Bufo species groups. Analyses of transferrins and hemoglobins, specifically, indicate that these components are highly variable. This paper presents my findings on the multiple molecular forms of plasma transferrin and hemoglobin in the six species of the B. americanus species group. These species are the American toad, B. americanus; the Dakota toad, B. hemiophrys; the Houston toad, B. houstonensis; the southwestern toad, B. microscaphus; the southern toad, B. terrestris; and the Rocky Mountain toad, B. woodhousii. The B. americanus group has been the subject of an intensive study of genetic compatibility relationships (W. F. Blair, 1963a). The significance of mating call as an ethological isolating mechanism in the group was discussed by W. F. Blair (1956, 1957a, 1957b, 1958), and ecological differences were found important in maintaining species integrity (A. P. Blair, 1941, 1955). Chromatograms of the parotoid gland secretions of various species in the group were examined by Hunsaker, et al. (1961) and Wittliff (1962). Abramoff, et al. (1964) used serology to test the relationships of B. americanus from islands in upper Lake Michigan. Blood proteins of some species were studied by Fox,

Electrophoretic Patterns of Plasma Proteins in the Viperine Snakes

Electrophoretic Patterns of Plasma Proteins in the Viperine Snakes

Electrophoretic Pattern of Plasma Proteins in Rats treated with Trypan Blue and Ethionine

IT is now well known that the liver plays a prominent part in the biosynthesis of the blood plasma proteins which is notably disturbed by every major hepatic injury. We have examined these phenomena in the Wistar rat treated by trypan blue and by ethionine. These substances are chemically different, but they have properties in common, such as hepatoxicity and carcinogenic power with a hepatic tropism. Trypan blue was given by intraperitoneal injections in a 0.5 per cent solution in sodium chloride 9 per cent, for 8 days; the daily dose was of 4 mgm./100 gm. of body-weight in a series of 7 animals and of 8 mgm./100 gm. of body-weight in a second series of 10 animals. These animals were male and were about 3 months old.

Unusual Electrophoretic Patterns of Plasma Proteins in Human Subjects

Abstract Study of 313 plasma electrophoretic patterns from 158 hospitalized subjects revealed that 30 per cent of these patients exhibited a heterogeneity marked by occurrence of double peaks in the alpha-2 and 6 per cent in the beta globulin fraction. Occurrence of double peaks could not be related to any specific disease. Similar study of plasma from 18 normal adult subjects did not reveal double peaks in any of the protein fractions. Existence of prealbumin components, with mobilities greater than that of the albumin, has been observed in 6 of 158 pathological subjects. Mobilities and concentrations of components x1, x2, and x3 are reported.

Dysproteinemia Induced in Vitro by Plasmin Digestion of Fibrinogen

Abstract 1. Electrophoretic investigation of split products of plasmin digestion of pure fibrinogen and of fibrinogen in a plasma medium was performed. It was shown that split products possessed electrophoretic mobilities of alpha, beta and gamma globulins. 2. It could be shown that by digestion of I131-labeled fibrinogen, radioactivity in the range of alpha, beta and gamma globulins is released. 3. Simultaneously with the formation of high molecular intermediates, low molecular, TCA-soluble substances are released. 4. Radioactivity balance calculation of the relative amounts of the newly formed fractions has been performed. 5. A possible influence of plasmin-induced fibrinogen proteolysis on the electrophoretic pattern of plasma proteins in dysproteinemia has been suggested. 6. The electrophoretic pattern of plasma proteins, obtained in the above experiments is similar to those seen in patients with dysproteinemia.

Electrophoretic pattern of plasma proteins in clinically diagnosed cases of kwashiorkor (protein malnutrition) and undernutrition (marasmus) among Ceylonese children.

Electrophoretic pattern of plasma proteins in clinically diagnosed cases of kwashiorkor (protein malnutrition) and undernutrition (marasmus) among Ceylonese children.

Characteristic Electrophoretic Patterns of Plasma Proteins of Orders of Amphibia and Reptilia

Characteristic Electrophoretic Patterns of Plasma Proteins of Orders of Amphibia and Reptilia

Electrophoretic patterns of the plasma proteins in diffuse liver necrosis

It is well recognized that changes in electrophoretic patterns of serum or plasma proteins occur in various types of liver diseases.l-l0 To date, however, only a few isolated studies of these proteins have been reported in patients with diffuse hepatic necrosis.s, 9 Systematic attempt has not yet been made to evaluate the results in a large series of proved cases. * We describe in the present paper the electrophoretic pattern of the plasma proteins seen in 10 cases of diffuse liver necrosis in all of which extensive anatomico-pathologic studies of the liver were made. The exact diagnosis of such cases being frequently impossible solely on the bases of the clinical picture and usual tests, we preferred grouping them according to the anatomicopathologic features, considering three criteria: (1) Diffuse or focal, according to the extension of the lesion throughout the liver as a whole. In the present studies we will consider only the diffuse cases. (2) Massive or zonal, according to the extension of the lesion throughout the hepatic lobule.n (3) Fulminant or protracted, according to the approximate duration of the lesions. We based the distinction between fulminant and protracted forms on the absence or presence of regeneration, degree of bile duct proliferation, reticulum collapse, and widening of sinusoids. However, no one of these criteria alone allows a positive judgment concerning the length of the process. In 5 cases (1. A. P., C. C., B. C. M., A. J., and J. A. Q.) the pathologic features were suggestive of a viral etiology, according to the criteria of Lucke and Mallory.13

Surgical Operation as Stress and its Influence on Electrophoretic Pattern of Blood Plasma Proteins

Changes in electrophoretic pattern of plasma proteins were studied in 11 cases of thracoplastik operations from the standpoint of regarding surgical operation as a stress. In addition, some indexes for adaptation responses (Ogawa's colloid reaction, Urinary glucose-total-reduction-power-ratio, etc.), changes of peripheral blood pictures, nitrogen excretion, and nitrogen balance were also studied.The changes in plasma proteins were classified into the following three groups;A: in which A/G was normal before operation, whereas decrease in Alb. and increase in total Glob. was obserdev after surgical operation.B: in which A/G was very low before operation, whereas increase in Alb. and decrease in total Glob. was observed after operation.C: in which A/G was low before operation, whereas decrease in both Alb. and total Glob. was observed after operation.In all cases, however, it was noted that the percentage of γ-Glob-fraction was definitely lowered, suggesting a_ catabolic effect by the surgical operation. These changes became maximum at the third day following the operation, when the changes in peripheral blood picture and all indexes of adaptation responses were already on the road of recovery while the urinary excretion of nitrogen and the negative nitrogen balance were at the highest levels.