Electron-multiplying Charge-coupled Device Research Articles

Real-time trajectory imaging of alpha particles emitted from actinium-225 and its daughter radionuclides

In targeted alpha-particle therapy, actinium-225 (Ac-225) has emerged as a radionuclide of potential, driving extensive efforts to develop innovative radiopharmaceuticals. High-resolution imaging of alpha particles is required for precisely detecting alpha-emitting radionuclides in cellular environments and small organs. Here, we report real-time trajectory imaging of alpha particles emitted by Ac-225 and its daughter radionuclides, utilizing an alpha particle trajectory imaging system. This system incorporates a magnification unit, a cooled electron-multiplying charge-coupled device (EM-CCD) camera, and a Ce-doped Gd3Al2Ga3O12 (GAGG) scintillator. Alpha particles were projected onto the GAGG scintillator, producing magnified images that were captured at 100 ms intervals. We successfully tracked particle trajectories with varying lengths and intensities for 4 different alpha particles emitted from Ac-225 and its daughter radionuclides with a spatial resolution of 1.0 μm. Notably, we achieved the imaging of sequentially emitted trajectories from Fr-221 and its decay product At-217, characterized by short decay intervals, along with the extended trajectories of high-energy alpha particles emitted by Po-213. These results demonstrate that high-resolution trajectory imaging, integrated with temporal and energy information, offers profound insights into the real-time behavior of Ac-225 and its daughter radionuclides within living cells or tissue sections, thereby driving advancements in targeted alpha-particle therapy.

DNA Hybridization Kinetics Observed at the Single-Molecule Level Using Graphene Near-Field Effects.

We present the development of an advanced sensing platform using a monolayer of graphene functionalized with fluorophore-labeled DNA hairpins to detect the kinetics of single hairpins during the hybridization reaction. The near-field photonic effects of graphene induce a distance-dependent quenching effect on the attached fluorescent labels, resulting in distinct optical signals in response to axial displacements resulting from DNA hybridization. Employing a wide-field Total Internal Reflection Fluorescence (TIRF) optical setup coupled with a sensitive Electron-Multiplying Charge-Coupled Device (EM-CCD) camera, we successfully detected fluorescent signals of individual or a low number of individual DNA hairpins within a low-concentration environment DNA target (tDNA). These signals were used to determine the optical setup's Point Spread Function (PSF) in a novel approach to super-resolution reconstruction. Combining these techniques, the subpixel localization of single hairpin molecules and their respective intensity profiles were extracted, enabling a kinetic assessment of individual DNA hairpins, with estimated unfolding times of approximately 7 s. Observations of kinetic phenomena unveiled intermediate partially hybridized states, extending the time required to unfold the hairpin probes by more than a factor of 2. Furthermore, a developed semiempirical model allowed the conversion of fluorescent signals into fluorophore-graphene distances. At the nanometer scale, we observed a step-like unfolding process characterized by intermittent metastates of unfolding and static periods, which can be attributed to nucleation events in some cases. Our graphene-based sensing platform and optical methodologies can be adopted for further research into the kinetics of different biomolecules under diverse environmental conditions.

Divertor tungsten source monitoring by A visible spectroscopy diagnostic on EAST

EAST has been upgraded with top and bottom tungsten divertors to facilitate the high power long pulse operation. A visible spectroscopy diagnostic has been developed progressively to monitor W source as well as line emissions from other particles (e.g., D, He, Li, B, C, N, O, Ne, Ar, Mo, D2, WD) in the divertors. Two sets of lens installed in mid-plane port view tangentially the top and bottom divertors, respectively. Two types of optical fibers transfer the plasma light collected by the lens to three different detecting modules. High spectral resolution (λ/Δλ ∼ 14000) can be achieved by the detecting module consisting of spectrometer and Electron-Multiplying Charge Coupled Device (EMCCD), high temporal resolution (50 μs) is obtained by filtered photoelectric multiplier (PMT), and high spatial resolution (1 mm) is acquired by Complementary Metal-Oxide-Semiconductor Transistor (CMOS) camera with collimation optics. The multichannel optical fiber array with 500 μm core diameter is used to connect the lens and the first two detecting modules. The imaging fiber bundle with 30 μm core diameter transmits the two-dimensional divertor plasma light to the third detecting module, i.e. collimation optics and CMOS camera. The diagnostic is operated automatically in normal EAST discharges to support experimental operation and divertor physics study. The paper presents a technical description of the diagnostic and typical measurements during EAST discharges.

High-Speed Fluctuation Analysis of Silver-Nanoparticle SERS in Solutions.

We analyzed the fluctuation of surface-enhanced Raman spectra with a temporal resolution of 25 ms using a conventional electron-multiplying charge-coupled device camera experimental setup. The signal-to-noise ratio of the spectra was improved using density-based spatial cluster analysis with noise. Silver nanoparticles (AgNPs) with different sizes were dispersed as surface-enhanced Raman spectroscopy platforms in violet aqueous solutions. The movement of AgNPs and the fluctuation of the spectra were characterized. The fluctuation (signal ON and OFF) was evaluated on the basis of the time intervals between ON and OFF timing. The behavior of each AgNP solution was explained by a two-dimensional random walk model, which means that the phenomenon was mainly governed by the Brownian motion of the AgNPs in the solution. The fluctuation was also compared among three different Raman modes, one of which showed anomalous behavior.

First spectroscopic study of HFRC plasma

An advanced spectral diagnostic system was developed to measure the electron temperature (T e), electron density (N e), and ion temperature (T i) of the Huazhong University of Science and Technology field-reversed configuration plasma. The system consists of an optic fiber spectrometer with a wide spectral band and a 670 mm focal length high throughout Czerny–Turner monochromator equipped with both a 3600 g mm−1 grating and a 2400 g mm−1 grating to measure the line spectrum. Accompanying these components is an electron-multiplying charge-coupled device camera to capture spectral data. The relative intensity of the optical fiber spectrometer was calibrated using a standard luminance source, and the wavelength calibration of the spectrometer was accomplished using a Hg/Ar lamp. This diagnostic setup was configured to measure electron density based on the Stark effect of Hγ (n = 5 → n = 2; 434.04 nm). Doppler broadening of an O III (2s22p(2P0)3p → 2s22p(2P0)3s; 375.988 nm) emission line was measured and analyzed to obtain the ion temperature, and electron temperatures were estimated from the relative strength of Hβ (n = 4 → n = 2; 486.14 nm) (Dβ) and Hγ (Dγ) spectral lines when the electron density was obtained from Stark effect measurements. The initial experimental results indicate that the highest electron temperature of the formation region was approximately 8 eV, the electron density of the colliding-and-merging region was approaching 1020 m−3, and the ion temperature reached about 40 eV.

Multifold enhancement of quantum SNR by using an EMCCD as a photon number resolving device

Electron multiplying charge-coupled devices (EMCCDs), owing to their high quantum efficiency and spatial resolution, are widely used to study typical quantum optical phenomena and related applications. Researchers have already developed a procedure that enables one to statistically determine whether a pixel detects a single photon, based on whether its output is higher or lower than the estimated noise level. However, these techniques are feasible at extremely low photon numbers (≈0.15 mean number of photons per pixel per exposure), allowing for at most one photon per pixel. This limitation necessitates a very large number of frames required for any study. In this work, we present a method to estimate the mean rate of photons per pixel per frame for arbitrary exposure time. Subsequently, we make a statistical estimate of the number of photons (≥ 1) incident on each pixel. This allows us to effectively use the EMCCD as a photon number resolving device. This immediately augments the acceptable light levels in the experiments, leading to significant reduction in the required experimentation time. As evidence of our approach, we quantify contrast in quantum correlation exhibited by a pair of spatially entangled photons generated by a spontaneous parametric down conversion process. In comparison with conventional methods, our method realizes an enhancement in the signal-to-noise ratio (SNR) by approximately a factor of 3 for half the data collection time. This SNR can be easily enhanced by minor modifications in experimental parameters such as exposure time, etc.

Enhancing electrochemiluminescence intensity through emission layer control

Electrochemiluminescence (ECL), an intriguing luminescent phenomenon induced by electrochemical stimulation, has evolved from studying electron transfer reactions to a powerful analytical method and imaging technique. ECL can be generated through annihilation or co-reactant methods, with recent advancements integrating it into imaging devices for diverse applications. This review traces the evolution of ECL from its early applications to recent developments in imaging technology. Notably, the utilization of charge-coupled devices (CCD) and electron multiplying charge-coupled devices (EMCCD) in ECL microscopy has revolutionized imaging capabilities, making it a cost-effective option for point-of-care testing. The review explores the heterogeneous ECL mechanism, emphasizing its limitations and challenges in visualizing objects away from the electrode surface. The synergy between ECL and microscopy is highlighted, showcasing its diverse applications and contributions to understanding ECL mechanisms and improving its use in biological contexts. Finally, the review encapsulates innovative approaches in material design, surface modification, and electrode architecture, providing a comprehensive overview of strategies to control the active layer of the ECL and advance ECL microscopy.

Photophysical image analysis: Unsupervised probabilistic thresholding for images from electron-multiplying charge-coupled devices.

We introduce the concept photophysical image analysis (PIA) and an associated pipeline for unsupervised probabilistic image thresholding for images recorded by electron-multiplying charge-coupled device (EMCCD) cameras. We base our approach on a closed-form analytic expression for the characteristic function (Fourier-transform of the probability mass function) for the image counts recorded in an EMCCD camera, which takes into account both stochasticity in the arrival of photons at the imaging camera and subsequent noise induced by the detection system of the camera. The only assumption in our method is that the background photon arrival to the imaging system is described by a stationary Poisson process (we make no assumption about the photon statistics for the signal). We estimate the background photon statistics parameter, λbg, from an image which contains both background and signal pixels by use of a novel truncated fit procedure with an automatically determined image count threshold. Prior to this, the camera noise model parameters are estimated using a calibration step. Utilizing the estimates for the camera parameters and λbg, we then introduce a probabilistic thresholding method, where, for the first time, the fraction of misclassified pixels can be determined a priori for a general image in an unsupervised way. We use synthetic images to validate our a priori estimates and to benchmark against the Otsu method, which is a popular unsupervised non-probabilistic image thresholding method (no a priori estimates for the error rates are provided). For completeness, we lastly present a simple heuristic general-purpose segmentation method based on the thresholding results, which we apply to segmentation of synthetic images and experimental images of fluorescent beads and lung cell nuclei. Our publicly available software opens up for fully automated, unsupervised, probabilistic photophysical image analysis.

A method for estimating the incident directions of alpha particles in 2-dimensional trajectory images in a GAGG plate

An imaging technique utilizing a scintillator plate in conjunction with a magnifying unit and a cooled electron multiplying charge-coupled device (EM-CCD) camera shows promise for capturing high-resolution trajectory images. Nevertheless, in the 2-dimensional trajectory images, the incident directions of the alpha particles entering the scintillator plate remained unknown due to the line-shaped trajectories. To elucidate the incident directions in our trajectory images, we conducted experiments capturing trajectory images of alpha particles under off-focus conditions. To capture off-focus images of alpha particles, we systematically varied the distance between the GAGG plate and the lens during imaging using an americium-241 (Am-241) source. Through images obtained at different distances between the GAGG plate and the lens, we successfully acquired trajectory images with varying degrees of off-focus, revealing that trajectory images focused on the upper surface of the GAGG plate exhibited blurred and wider trajectories in the deeper regions, making the incident directions of the alpha particles evident. We conclude that the proposed off-focus method for trajectory imaging of alpha particles holds promise for estimating the incident directions in the trajectory images.

Recovery of polarization entanglement in partially coherent photonic qubits.

Partially coherent photonic qubits, owing to their robustness in propagation through random media compared to fully coherent qubits, find applications in free-space communication, quantum imaging, and quantum sensing. However, the reduction of spatial coherence degrades entanglement in qubits, adversely affecting entanglement-based applications. We report the recovery of entanglement in the partially coherent photonic qubits generated using a spontaneous parametric downconversion process despite retaining their multimode nature. This study utilizes an electron multiplying charge-coupled device (EMCCD) to perform coincidence measurements, eliminating the need for raster scanning of single-pixel detectors, which simplifies optical alignment, enhances precision, and reduces time consumption. We demonstrate that the size of apertures used to select biphotons substantially impacts the visibility and S-parameter of polarization-entangled partially coherent qubits. The entanglement is recovered with partial spatial coherence properties by choosing small sizes of the apertures in the captured image plane. This study could help in the advancement of free-space quantum communication, quantum imaging, and quantum metrology.

Chasing snails: Automating the processing of EMCCD images of luminescence from opercula

Opercula of the gastropod Bithynia tentaculata are composed of calcite, and are typically 2–4 mm in length. They emit a thermoluminescence (TL) signal that can be used for dose reconstruction, and spatially resolved TL data from them can be obtained using an electron multiplying charge coupled device (EMCCD). However, when multiple measurements are made of the same sample with imaging detectors such as the EMCCD, registering the different images is crucial so that when regions of interest (ROI) are defined they consistently relate to the same part of the specimen. Previous work on opercula has undertaken this registration by hand, but this is prohibitively time consuming and is also potentially prone to human error. An automated registration process is described, and its use is illustrated using a dose recovery experiment. Without registration more than half of the regions of interest defined across the operculum failed the recycling test, and for those ROIs which did pass recycling the dose recovery ratio varied from 0.7 to 1.2. After registration more than 97% of ROIs passed recycling and all these ROIs gave dose recovery ratios within two sigma of unity. The automated registration process described here has potential for application to other types of solid sample such as rock slices provided they are not perfectly circular.

A comparative study of EM-CCD and CMOS cameras for particle ion trajectory imaging

High-resolution and real-time imaging of particle ion trajectories is essential in nuclear medicine and nuclear engineering. One potential method to achieve high-resolution real-time trajectory imaging of particle ions involves utilizing an imaging system that integrates a scintillator plate with a magnifying unit and a cooled electron multiplying charge-coupled device (EM-CCD) camera. However, acquiring an EM-CCD camera might prove challenging due to the discontinuation of CCD sensor manufacturing by vendors. As an alternative imaging approach, a low-noise, high-sensitivity camera utilizing a cooled complementary metal-oxide-semiconductor (CMOS) sensor offers a promising solution for imaging particle ion trajectories. Yet, it remains uncertain whether CMOS-based cameras can perform as effectively as CCD-based cameras in capturing particle ion trajectories. To address these concerns, we conducted a comparative analysis of the imaging performance between a CMOS-based system and an EM-CCD-based system for capturing alpha particle trajectories. The results revealed that both systems could image the trajectories of alpha particle, but the spatial resolution with the CMOS-based camera exceeded that of the EM-CCD-based camera, primarily due to the smaller pixel size of the sensor. While the signal-to-noise ratio (SNR) of the trajectory image from the CMOS-based camera initially lagged behind that from the EM-CCD-based camera, this disparity was mitigated by implementing binning techniques on the CMOS-based camera images. In conclusion, our findings suggest that a cooled CMOS camera could serve as a viable alternative for imaging particle ion trajectories.

A preliminary study of multispectral Cherenkov imaging and a Fricke-xylenol orange gel film (MCIFF) for online, absolute dose measurement.

Cherenkov luminescence imaging has shown potential for relative dose distribution and field verification in radiation therapy. However, to date, limited research utilizing Cherenkov luminescence for absolute dose calibration has been conducted owing to uncertainties arising from camera positioning and tissue surface optical properties. This paper introduces a novel approach to multispectral Cherenkov luminescence imaging combined with Fricke-xylenol orange gel (FXG) film, termed MCIFF, which can enable online full-field absolute dose measurement. By integrating these two approaches, MCIFF allows for calibration of the ratio between two spectral intensities with absorbed dose, thereby enabling absolute dose measurement. All experiments are conducted on a Varian Clinac 23EX, utilizing an electron multiplying charge-coupled device (EMCCD) camera and a two-way image splitter for simultaneous capture of two-spectral Cherenkov imaging. In the first part of this study, the absorbance curves of the prepared FXG film, which receives different doses, are measured using a fluorescence spectrophotometer to verify the correlation between absorbance and dose. In the second part, the FXG film is positioned directly under the radiation beam to corroborate the dose measurement capacity of MCIFF across various beams. In the third part, the feasibility of MCIFF is tested in actual radiotherapy settings via a humanoid model, demonstrating its versatility with various radiotherapy materials. The results of this study indicate that the logarithmic ratios of spectral intensities at wavelengths of 550±50 and 700±100nm accurately reflect variations in radiation dose (R2>0.96) across different radiation beams, particle energies, and dose rates. The slopes of the fitting lines remain consistent under varying beam conditions, with discrepancies of less than 8%. The optical profiles obtained using the MCIFF exhibit a satisfactory level of agreement with the measured results derived from the treatment planning system (TPS) and EBT3 films. Specifically, for photon beams, the lateral distances between the 80% and 20% isodose lines, referred to as the penumbra (P80-20) values, obtained through TPS, EBT3 films, and MCIFF, are determined as 0.537, 0.664, and 0.848cm, respectively. Similarly, for electron beams, the P80-20 values obtained through TPS, EBT3 films, and MCIFF are found to be 0.432, 0.561, and 0.634cm, respectively. Furthermore, imaging of the anthropomorphic phantom demonstrates the practical application of MCIFF in real radiotherapy environments. By combining an FXG film with Cherenkov luminescence imaging, MCIFF can calibrate Cherenkov luminescence to absorbed dose, filling the gap in online 2D absolute dose measurement methods in clinical practice, and providing a new direction for the clinical application of optical imaging to radiation therapy.

Range and light output measurements of trajectory images in a GAGG plate with different alpha particle energies

An imaging method that utilizes a scintillator plate combined with a magnifying unit and a cooled electron multiplying charge-coupled device (EM-CCD) camera shows promise for obtaining high-resolution trajectory images. However, it is not yet clear whether the ranges of the trajectory images change with the energy of the alpha particles. Additionally, it remains unclear whether the intensity of the trajectory images is affected by the energy of the alpha particles. To address these questions in our trajectory imaging research, we conducted experiments to capture trajectory images of alpha particles with varying energy levels. To generate alpha particles with different energies, we modulated the energy using an americium-241 (Am-241) source covered with varying numbers of Mylar films. With this alpha source and imaging system, we successfully captured trajectory images with different alpha particle energies and were able to assess the ranges and intensities of these trajectories at various energy levels. The estimated ranges from the measured images with different alpha particle energies closely matched the results obtained through simulations. However, it's worth noting that the light output, as evaluated for the measured trajectory images, was slightly lower than the simulated results at lower energy levels probably due to the non-proportionality of the GAGG plate with respect to alpha particle energies.

Design of a Scanning Module in a Confocal Microscopic Imaging System for Live-Cell Imaging

This study proposes a Nipkow-based pinhole disk laser scanning confocal microscopic imaging system for ordinary optical microscopy, fluorescence microscopy, and confocal microscopy imaging of biological samples in order to realize the dynamic experimental monitoring of space-based life science experiments and the fine observation of biological samples. Confocal microscopic imaging is mainly completed by a scanning module that is composed of a spinning disk and other components. The parameters of the spinning disk directly determine the quality of the image. During the design process, the resolution and signal-to-noise ratios caused by different pinhole diameters in the spinning disk are the main considerations. Changes and image blurring caused by crosstalk due to the pinhole arrangement and different pinhole spacings are addressed. The high photon efficiency of the new EMCCD (electron-multiplying charge-coupled device) and CMOS (complementary metal-oxide-semiconductor) camera reduces the exposure time as much as possible, reduces damage to living cells, and achieves high-speed confocal imaging. It is shown in a confocal imaging experiment with a variable magnification of 1–40× that the imaging resolution of the system can reach a maximum of 2592 × 1944, the spatial resolution can reach 1 μm, and the highest sampling frequency is 10 fps, thus meeting the design requirements for high-speed live-cell imaging.

Using machine learning to improve multi-qubit state discrimination of trapped ions from uncertain EMCCD measurements.

This paper proposes a residual network (ResNet)-based convolutional neural network (CNN) model to improve multi-qubit state measurements using an electron-multiplying charge-coupled device (EMCCD). The CNN model is developed to simultaneously use the intensity of pixel values and the shape of ion images in determining the quantum states of ions. In contrast, conventional methods use only the intensity values. In our experiments, the proposed model achieved a 99.53±0.14% mean individual measurement fidelity (MIMF) of 4 trapped ions, reducing the error by 46% when compared to the MIMF of maximum likelihood estimation method of 99.13±0.08%. In addition, it is experimentally shown that the model is also robust against the ion image drift, which was tested by intentionally shifting the ion images.

Ultrahigh resolution real-time trajectory imaging of neutron induced particles in a scintillator from lithium-6 plate

It is known that a lithium-6 (6Li) absorbs a neutron and is divided into a triton and an alpha particle. However, the trajectories of the produced tritons have not yet been imaged in real time and high resolution. We developed an ultrahigh-resolution imaging system that can clearly observe the trajectories of neutron induced particles in real time. The developed system is based on a magnifying unit and a cooled electron multiplying charge-coupled device (EM-CCD) camera combined with a 6Li plate and a Ce-doped Gd3Al2Ga3O12(GAGG) scintillator plate. Neutrons from a californium-252 (252Cf) source were irradiated to the 6Li plate, which produced tritons and alpha particles. The produced tritons or alpha particles entered the GAGG plate and produced scintillation light along the trajectories. The scintillation trajectories were magnified by the unit, light intensified, and imaged by the EM-CCD camera. Using our system, we could measure the elongated trajectory images of the particles in real time. Most of these trajectories had Bragg peak like shapes in the images. The average range was 15 μm and the width was 4.6 μm FWHM. From the ranges we estimated, we found that these trajectories could be attributed to the induced tritons. Consequently, the developed real time imaging system is promising for research on the ultrahigh resolution imaging of neutron produced particles.

Sub-micrometer real-time imaging of trajectory of alpha particles using GAGG plate and CMOS camera

High-resolution and real-time imaging of the trajectories of alpha particles is desired in nuclear medicine and nuclear engineering. Although an imaging method using a scintillator plate combined with a magnifying unit and a cooled electron multiplying charge-coupled device (EM-CCD) camera is a possible method of obtaining high-resolution trajectory images, the spatial resolution of the system is limited to ∼2 μm. To overcome the spatial resolution limitations of this method on trajectory imaging, we used a cooled complementally metal oxide (CMOS) camera in which the sensor had a much larger number of pixels, which were also smaller. Using the CMOS camera based imaging system, we could measure the trajectories of alpha particles in real time with the spatial resolution of 0.34 μm FWHM. With smoothing of the images to reduce image noise, spatial resolution was still kept to less than 0.75 μm. We conclude that this CMOS camera-based alpha-particle trajectory-imaging system is promising for alpha-particle or other particles imaging where ultrahigh spatial resolution is required.

Optimal sparsity allows reliable system-aware restoration of fluorescence microscopy images.

Fluorescence microscopy is one of the most indispensable and informative driving forces for biological research, but the extent of observable biological phenomena is essentially determined by the content and quality of the acquired images. To address the different noise sources that can degrade these images, we introduce an algorithm for multiscale image restoration through optimally sparse representation (MIRO). MIRO is a deterministic framework that models the acquisition process and uses pixelwise noise correction to improve image quality. Our study demonstrates that this approach yields a remarkable restoration of the fluorescence signal for a wide range of microscopy systems, regardless of the detector used (e.g., electron-multiplying charge-coupled device, scientific complementary metal-oxide semiconductor, or photomultiplier tube). MIRO improves current imaging capabilities, enabling fast, low-light optical microscopy, accurate image analysis, and robust machine intelligence when integrated with deep neural networks. This expands the range of biological knowledge that can be obtained from fluorescence microscopy.

Rapid Imaging Planetary Spectrograph

The Rapid Imaging Planetary Spectrograph (RIPS) was designed as a long-slit high-resolution spectrograph for the specific application of studying atmospheres of spatially extended solar system bodies. With heritage in terrestrial airglow instruments, RIPS uses an echelle grating and order-sorting filter to obtain optical spectra at resolving powers of up to R ∼ 127,000. An ultra-narrowband image from the reflective slit jaws is captured concurrently with each spectrum on the same electron-multiplying charge-coupled device detector. The “rapid” portion of RIPS’s moniker stems from its ability to capture high frame rate data streams, which enables the established technique known as “lucky imaging” to be extended to spatially resolved spectroscopy. Resonantly scattered emission lines of alkali metals, in particular, are sufficiently bright to be measured within short integration times. RIPS has mapped the distributions of Na and K emissions in Mercury’s tenuous exosphere, which exhibits dynamic behavior coupled with the planet’s plasma and meteoroid environment. An important application is daylight observation of Mercury with solar telescopes, as the synoptic context of the exosphere’s distribution comprises valuable ground-based support for the upcoming BepiColombo orbital mission. As a conventional long-slit spectrograph, RIPS has targeted the Moon’s surface-bound exosphere, where structures in line width and brightness are observed as a function of tangent altitude. At the Galilean moons, RIPS can study the plasma interaction with Io and place new constraints on the sputtered atmosphere of Europa, which in turn provides insight into the salinity of Europa’s subsurface ocean. The instrumental design and construction are described herein, and these astronomical observations are presented to illustrate the performance of RIPS as a visiting instrument at three different telescope facilities.