Latent fingermark imaging plays an important role in forensic investigation. The choice of an appropriate method and reagent is essential for acquiring high quality fingermark images. In this work, a push-pull structure NIFA of a fluorene (π-conjugated) core substituted with 1,8-naphthalimide (electron-attracting group) and anthracene (electron-donating group) was fabricated, which provided an aggregation-enhanced emission (AEE). The photophysical and AEE properties of NIFA were investigated by experimental and theoretical studies. This dye can emit bright blue to blue-green fluorescence. The results showed that the intramolecular charge transfer from the fluorene part to the 1,8-naphthalimide part dominated the luminescence of NIFA. The combined utilization of cyanoacrylate glue fuming and a NIFA fluorescent imaging technique can image the fine structure of fingermark from the first level to the third level. After investigating the interaction mechanism between cyanoacrylate glue and NIFA, we found that the hydrophobic-hydrophobic interaction was in charge of the fingermark imaging quality enhancement. All results proved that this AEE active fluorescent probe combined with cyanoacrylate fuming could supply a new method for latent fingermark fluorescent detection.