Electrodes For Electrochemical Sensors Research Articles

Green synthesis of Oscimum Sanctum mediated silver nanoparticles to fabricate sensors for hydrogen peroxide detection

In this study, silver nanoparticles (Ag NPs) of an average size of ∼15 nm have been produced using the green synthesis technique with Oscimum Sanctum leaf extract. Further, these nanoparticles have been used to prepare electrodes for electrochemical sensors to monitor hydrogen peroxide. Hydrogen peroxide sensors have many applications in different sectors, such as medical, agriculture, and industry. The optical and structural characteristics of the Ag NPs have been investigated by UV–visible absorption spectroscopy and XRD patterns. Particle size, shape, and morphology of as-synthesized Ag NPs have been analyzed using transmission and scanning electron microscopic techniques. These nanoparticles were deposited onto an indium-tin-oxide glass substrate using the electrophoretic technique, which was used as an electrode to assess the electro-oxidation of Ag NPs by cyclic voltammetry. The sensitivity of the sensor has been estimated to be 4.16 µA/mM-cm2. The limit of detection of the sensor is estimated to be 40 µM within the linear range of 5–28 mM. The lattice parameter, interplanar spacing, and crystallite size of Ag NPs have been quantified and estimated against pH variation.

Fabrication of Silver Nanoparticle Loaded into Nanocellulose Derived from Hemp and Poly(vinyl alcohol)-Based Composite as an Electrode for Electrochemical Sensors for Lactate Determination.

Nanocellulose derived from hemp (HNC) with the addition of silver nanoparticles (AgNPs) is utilized for improving the electrochemical sensing performances for lactate detection. Initially, HNC is chemically extracted and purified by using alkali treatment and acid hydrolysis. Then, AgNPs are nucleated in situ by the self-reduction process prior to forming a composite with poly(vinyl alcohol) (PVA). This nanocomposite significantly improves the electrochemical properties of the electrode, including electrochemical conductivity and electrocatalysis. The morphologies and chemical alterations of the HNC/AgNPs-PVA nanocomposite are investigated by field emission scanning electron microscopy. It demonstrates a three-dimensional network with random orientation of the nanocellulose fiber. The AgNPs are well-dispersed in the nanocomposite. Moreover, the nanocomposite provides high thermal stability up to 450 °C. Then, it is remarkably noted that 10 wt % HNC/AgNPs-PVA modified on the electrode provides the highest current responses, with a standard redox couple [(Fe(CN)6]3-/4-]. For lactate detection, this modified screen-printed graphene electrode with nonimmobilized lactate oxidase exhibits an increase in the current signal with the increment of lactate concentration and offered a linear range of 0-25 mM, covering a cutoff value (12.5 mM) for muscle fatigue indication. Eventually, this sensor is successfully applied for lactate detection with high potential for a wearable lactate sensor.

Efficient Amperometric Detection of H2O2 using Gold Nanoparticle decorated Polythiophene/Hematite Ore Nanocomposite

In the present work, we developed a cheap and sensitive H2O2 electrochemical sensor. Herein we fabricated an electrochemical sensor electrode using a naturally extracted hematite ore decorated with conducting polythiophene (Pth) and gold nanoparticles (AuNPs). A simple synthesis route was adopted for the electrocatalyst synthesis, where Pth was synthesized through oxidative polymerization and then combined with Hematite Ore nanostructure via a simple ultrasonication process. Later a simple photo-reduction approach was used to develop a 1%Au@5%Pth/Hematite Ore nanocomposite. The as-fabricated Au@Pth/Hematite Ore nanocomposite was successfully characterized by applying X-ray diffraction (XRD), X-ray Photoelectron Spectroscopy (XPS), High-Resolution Transmission Electron Microscope (HR-TEM), and Field Emission Scanning Electron Microscope (FE-SEM) techniques. The obtained results reveal that undoped naturally extracted hematite ore is composed of Fe2O3 and Fe3O4 phases. The catalytic efficiency of the newly designed nanocomposite and its sensing ability towards H2O2 were assessed using electrochemical techniques including electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), linear sweep voltammetry (LSV) and highly sensitive amperometric (i-t) techniques. The Au@Pth/Hematite Ore/GCE sensor showed a wide linear dynamic range of 0.50–9.50 mM with high sensitivity of 69.18 μAmM−1cm−2. The limit of detection (LOD) was estimated to be 5.18 μM. The examined sensor demonstrated acceptable reproducibility, repeatability as well as stability. The sensor electrode also showed anti-interference behavior in the presence of different inorganic and organic interfering ions or molecules during the H2O2 determination. Moreover, the proposed sensor exhibits acceptable recovery of H2O2 in real sample analysis. Hence, this novel sensor is regarded as a promising contender in scientific and industrial domains.

Conductive Diamond-like Carbon Microneedle Electrode for Electrochemical Sensors

Conductive Diamond-like Carbon Microneedle Electrode for Electrochemical Sensors

A Highly Sensitive Non-Enzymatic Glucose Electrochemical Sensor Electrode Material of CuO Nanospheres/Activated Carbon Composites

A Highly Sensitive Non-Enzymatic Glucose Electrochemical Sensor Electrode Material of CuO Nanospheres/Activated Carbon Composites

A Study on the O2 Plasma Etching Method of Spray-Formed SWCNT Films and Their Utilization as Electrodes for Electrochemical Sensors.

In this study, we analyzed the morphological changes and molecular structure changes on the surface of single-walled carbon nanotube (SWCNT) films during oxygen plasma (O2) etching of SWCNT surfaces formed by the spray method and analyzed their potential use as electrochemical electrodes. For this purpose, a SWCNT film was formed on the surface of a glass substrate using a self-made spray device using SWCNT powder prepared with DCB as a solvent, and SEM, AFM, and XPS analyses were performed as the SWCNT film was O2 plasma etched. SEM images and AFM measurements showed that the SWCNT film started etching after about 30 s under 50 W of O2 plasma irradiation and was completely etched after about 300 s. XPS analysis showed that as the O2 plasma etching of the SWCNT film progressed, the sp2 bonds representing the basic components of graphite decreased, the sp3 bonds representing defects increased, and the C-O, C=O, and COO peaks increased simultaneously. This result indicates that the SWCNT film was etched by the O2 plasma along with the oxygen species. In addition, electrochemical methods were used to verify the damage potential of the remaining SWCNTs after O2 plasma etching, including cyclic voltammetry, Randles plots, and EIS measurements. This resulted in a reversible response based on perfect diffusion control in the cyclic voltammetry, and an ideal linear curve in the Randles plot of the peak current versus square root scan rate curve. EIS measurements also confirmed that the charge transfer resistance of the remaining SWCNTs after O2 plasma etching is almost the same as before etching. These results indicate that the remaining SWCNTs after O2 plasma etching do not lose their unique electrochemical properties and can be utilized as electrodes for biosensors and electrochemical sensors. Our experimental results also indicate that the ionic conductivity enhancement by O2 plasma can be achieved additionally.

Fiber Based Devices for Sensing and Actuating Applications

The importance of multifunctional smart medical devices increases constantly during the last decades, fuelled by socio - economic challenges such as population aging and high costs of the healthcare system. Consequently, numerous research teams are focused on developing such devices with multiple functionalities in terms of both diagnostic and therapeutical approaches.Wearables become ubiquitous since computing and communication devices got smaller and more powerful, new functions being constantly added to consumer products such as smart watches or fitness monitors. Physical or chemical parameters including pulse, blood pressure or oxygen saturation are easily measured. Employing complex architectures based on nanostructures and materials with tailored properties represents an important path in developing a new generation of wearable medical devices which may both monitor chemical parameters (sweat or blood composition) and deliver various therapies.The present report focuses on describing such architectures with the potential of incorporating both health monitoring, transdermal drug delivery and other therapeutic functions in a relatively straightforward manner. As functional elements microscopic fibers are employed as both electrodes for electrochemical sensors, as microscopic heaters for the drug delivery function or, as building blocks for other specific functions. The fibers are fabricated using electrospinning or centrifugal/force spinning and functionalization (e.g. electrical conductivity) is achieved through physical and electrochemical deposition. Therefore, the fibers can be covered with thin metal layers and rendered conductive, oxidic or polymeric layers making them responsive to various stimuli. Heating is achieved in a straightforward manner through Joule effect and the use of a temperature responding hydrogel enables a controlled release of specific drugs while simultaneously improving epidermal permeation.These fiber-based systems can be easily employed on cheap, biocompatible substrates such as paper or textile materials and therefore can be mass produced with reasonable costs leading to a new generation of medical devices with increased functionality.

The Dependence of Boron Concentration in Diamond Electrode for Ciprofloxacin Electrochemical Sensor Application

This study investigates the effects of boron concentration on boron-doped diamond (BDD) electrodes for electrochemical sensors of ciprofloxacin. The effects of boron concentration, scan rate, and pH of BDD electrodes with boron concentrations of 0.1, 0.5, and 1% were examined to determine the optimal conditions. Furthermore, square wave voltammetry (SWV) in phosphate buffer pH 7 was used to analyze the electrochemical behavior of ciprofloxacin. The results revealed a linear calibration curve in the concentration range of 30–100 μM with a recovery of 85–110%. Meanwhile, BDD electrode with the highest boron concentration in this experiment (1%) showed a very low limit of detection of 0.17 μM, meaning that 1% BDD gave a highly sensitive and significant measurement result for the electrochemical sensor of ciprofloxacin. With the results given, this study provides new insights for controlling boron concentrations in diamond electrodes for the electrochemical sensors of quinolone antibiotics.

Facile synthesis of nickel-decorated multidimensional carbon nanofibers via oxygen plasma activation for non-enzymatic acetaminophen sensing

Acetaminophen (AMP), the most commonly used drug for pain and fever, has very low side effects. However, due to its widespread use, emissions from manufacturing plants and daily life adversely affect various animals and plants, so it is necessary to precisely monitor the concentration. This research suggests the manufacture of composite carbon nanofibers with carbon nanotubes containing nickel particles (Ni–CNT@CNF), and applying the material as an electrochemical sensor electrode material that is capable of measuring AMP. In detail, an oxygen functional group is introduced into the PVP layer by using an O2 vacuum plasma process in a polymer fiber formed by electrospinning a mixed solution of PAN and a PVP including a nickel precursor. The carbon nanotubes are then induced to grow by the catalytic reaction of nickel and decomposed PVP during the carbonization. The Ni–CNT@CNF-based sensor electrode has a low detection limit (0.5 nM), and a wide detection range of (1 nM–70 μM) for AMP molecules. In addition, comparison of sensitivity with other biologically active metabolites demonstrated the excellent selectivity of the sensor electrode.

One-step synthesis of 2D@3D hollow Prussian blue analogue as a high-performance bifunctional electrochemical sensor.

Prussian blue analogues (PBAs) are a family of classic coordination polymers. They have been widely applied in various fields including electrochemical sensors. Cubic nanoparticle structure is their common morphology. It is still a great challenge to design a hollow and two-dimensional (2D) PBA material. Of course, it will be a significant surprise if a hollow cube and 2D sheet can be integrated into one material. In this work, we designed a simple one-step synthetic strategy and resolved the above difficulty, wherein a hollow cubic PBA covered by 2D ultrathin nanosheets was successfully constructed, namely hollow tremella-like PBA (HTPBA). Furthermore, Ni foam (NF) as a substrate was introduced to obtain a self-supporting HTPBA/NF-12 electrode. HTPBA/NF-12, as a bifunctional electrochemical sensor electrode, exhibited distinguished catalytic performance towards glucose and nitrite, including remarkable selectivity, reproducibility, sensitivity for glucose (21 410 μA mM-1 cm-2) and nitrite (1248 μA mM-1 cm-2), wide linear range of 2-1250 μM and 5-3380 μM, along with low detection limit of 0.056 μM and 0.38 μM, respectively. More importantly, HTPBA/NF-12 electrodes possessed good reusability and practicability even in goat serum. In this study, we developed a simple and effective strategy to fabricate 2D@3D PBA material with excellent electrocatalytic activity and provide a totally new viewpoint in the PBA sensing field.

A Low-Cost Electrochemical Method for the Determination of Sulfadiazine in Aquaculture Wastewater.

As the concept of green development spreads worldwide, environmental protection awareness for production and life has been continuously strengthened. Antibiotic residues in aquaculture wastewaters aggravate environmental pollution and threaten human health. Therefore, the detection of residual antibiotics in wastewater is crucial. In this paper, a new, simple, and low-cost method based on the glassy carbon electrode electrochemical sensor for the detection of sulfadiazine in aquaculture wastewater was developed without using complex materials to modify the electrode surface, to detect sulfadiazine which electrochemically oxidizes directly. The electrochemical performance of the sensor was studied and optimized with differential pulse voltammetry and cyclic voltammetry in the three-electrode system. The optimal electrolyte was acetic acid-sodium acetate buffer, and the optimal pH was 4.0. Finally, based on the optimized conditions, the newly established method showed satisfactory results for detecting sulfadiazine in aquaculture wastewater. The concentration of sulfadiazine and the peak current intensity showed a linear relationship in the range of 20 to 300 μmol/L, and the limit of detection was 6.14 μmol/L, the recovery rate of standard addition was 87-95%, with satisfactory reproducibility and low interference.

3-D Electrodes for Electrochemical Sensors: Review in Different Approaches

With the demand for miniaturized devices, the development of 3-D-printing (3-DP) techniques to create sensors has emerged in the recent decade. 3-DP is not the only technique used to construct 3-D sensors (3-DS) and 3-D structures created from composites but also used for this purpose. This review reveals the most used technologies for the creation of electrochemical sensors, where 3-D electrodes (3-DE) are involved: 3-DP, plating, and composite formation. Fabrication and applications reported in the years 2020 and 2021 are summarized, and clairvoyance for most perspective directions is given.

Water Peel-Off Transfer of Electronically Enhanced, Paper-Based Laser-Induced Graphene for Wearable Electronics.

Laser-induced graphene (LIG) has gained preponderance in recent years, as a very attractive material for the fabrication and patterning of graphitic structures and electrodes, for multiple applications in electronics. Typically, polymeric substrates, such as polyimide, have been used as precursor materials, but other organic, more sustainable, and accessible precursor materials have emerged as viable alternatives, including cellulose substrates. However, these substrates have lacked the conductive and chemical properties achieved by conventional LIG precursor substrates and have not been translated into fully flexible, wearable scenarios. In this work, we expand the conductive properties of paper-based LIG, by boosting the graphitization potential of paper, through the introduction of external aromatic moieties and meticulous control of laser fluence. Colored wax printing over the paper substrates introduces aromatic chemical structures, allowing for the synthesis of LIG chemical structures with sheet resistances as low as 5 Ω·sq-1, translating to an apparent conductivity as high as 28.2 S·cm-1. Regarding chemical properties, ID/IG ratios of 0.28 showcase low defect densities of LIG chemical structures and improve on previous reports on paper-based LIG, where sheet resistance has been limited to values around 30 Ω·sq-1, with more defect dense and less crystalline chemical structures. With these improved properties, a simple transfer methodology was developed, based on a water-induced peel-off process that efficiently separates patterned LIG structures from the native paper substrates to conformable, flexible substrates, harnessing the multifunctional capabilities of LIG toward multiple applications in wearable electronics. Proof-of concept electrodes for electrochemical sensors, strain sensors, and in-plane microsupercapacitors were patterned, transferred, and characterized, using paper as a high-value LIG precursor for multiples scenarios in wearable technologies, for improved sustainability and accessibility of such applications.

Acicular Nickel on Carbon Nanofiber as Electrochemical Sensor Electrode for the Rapid Detection of Aqueous and Gaseous Formaldehyde at Room Temperature

AbstractThe rapid and straightforward detection of formaldehyde (FA) in the environment is crucial for preventing the accidental inhalation of FA and limiting skin exposure to FA. In this study, we developed a simple nickel‐based electrocatalytic electrode on carbon nanofibers (CNFs−Ni), which is suitable for rapidly detecting FA at room temperature. Centrifugal electrospinning was used to obtain polyacrylonitrile (PAN) nanofibers, which was subsequently stabilized and carbonized to fabricate the CNFs. Carbonization of the CNFs occurred at various temperatures (Tc=1200, 1300, 1400, and 1500 °C). PAN CNFs served as a highly conductive template for electroless plating under a magnetic field of 500 G to grow acicular nickel. The amperometric responses of the CNFs−Ni to aqueous FA were then measured. A lab‐built amperometric gas sensor (CNFs−Ni 1–8), which comprised CNFs with a reduced Ni loading, was used as the electrode for detecting gaseous FA. Scanning electron microscopy (SEM), linear sweep voltammetry (LSV), cyclic voltammetry (CV), and chronoamperometry were used to evaluate the sensitivities of the electrodes. Within the linear range of 0.05–91.5 mM, the CNFs1400‐Ni electrode was highly sensitive for detecting aqueous FA (2592 μA mM−1 cm−2), as evidenced by the fast response time (6 s). At a low concentration of gaseous FA (0.5 ppm), the laboratory‐built FA gas sensor was stable (98.3 %) and had a fast response time (5 s) after 9 h of continuous operation.

Stability Enhancement of Laser-Scribed Reduced Graphene Oxide Electrodes Functionalized by Iron Oxide/Reduced Graphene Oxide Nanocomposites for Nitrite Sensors

An iron oxide/reduced graphene oxide (ION-RGO) nanocomposite has been fabricated to functionalize a low-cost electrochemical nitrite sensor realized by light-scribed reduced graphene oxide (LRGO) electrodes on a PET substrate. To enhance the stability and adhesion of the electrode, the PET substrate was modified by RF oxygen plasma, and a thin layer of the cationic poly (diallyl dimethyl ammonium chloride) was deposited. Raman spectroscopy and scanning electron microscopy coupled to energy-dispersive X-ray spectroscopy (SEM-EDX) reveal that the light-scribing process successfully reduces graphene oxide while forming a porous multilayered structure. As confirmed by cyclic voltammetry, the LRGO electrochemical response to ferri-ferrocyanide and nitrite is significantly improved after functionalization with the ION-RGO nanocomposite film. Under optimized differential pulse voltammetry conditions, the LRGO/ION-RGO electrode responds linearly (R2 = 0.97) to nitrite in the range of 10–400 µM, achieving a limit of detection of 7.2 μM and sensitivity of 0.14 µA/µM. A single LRGO/ION-RGO electrode stands for 11 consecutive runs. The novel fabrication process leads to highly stable and reproducible electrodes for electrochemical sensors and thus offers a low-cost option for the rapid and sensitive detection of nitrite.

Electrochemical multi-sensors obtained by applying an electric discharge treatment to 3D-printed poly(lactic acid)

Electrochemical sensors for real-time detection of several bioanalytes have been prepared by additive manufacturing, shaping non-conductive poly(lactic acid) (PLA) filaments, and applying a physical treatment to create excited species. The latter process, which consists of the application of power discharge of 100 W during 2 min in a chamber at a low pressure of O2, converts electrochemically inert PLA into an electrochemically responsive material. The electric discharge caused the oxidation of the PLA surface as evidenced by the increment in the quantity of oxygenated species detected by FTIR spectroscopy and X-ray photoelectron spectroscopy (XPS). Indeed, changes in the surface chemical composition became more pronounced with increasing O2 pressure. After demonstrating the performance of the chemically modified material as individual dopamine and glucose sensors, multiplexed detection has been achieved by measuring simultaneously the two voltammetric signals. This has been performed by collecting the signals in two different regions, a naked chemically modified PLA for dopamine detection and a chemically modified PLA region functionalized with Glucose Oxidase. These outcomes led to define a new paradigm for manufacturing electrodes for electrochemical sensors based on 3D printing without using conducting materials at any stage of the process.

Effect of the Ni3TeO6 phase in a Ni2Te3O8/expanded graphite composite on the electrochemical monitoring of metribuzin residue in soil and water samples

Growing food demand and climate change have led to the development of various pest control agents to increase crop yields. Although pesticides help meet the food demand, they cause harm to human health and the environment. Metribuzin (MTBZ) is one of the common herbicides used for controlling weeds. Therefore, monitoring MTBZ residues in soil and water bodies is essential for decreasing risk to the environment and human health. This paper reports a highly selective and sensitive electrochemical sensor electrode based on a Ni3TeO6-phase-integrated Ni2Te3O8/expanded graphite (referred to here as NTO-eGR) composite for the detection of MTBZ. The NTO-eGR composite was prepared by a one-step low-temperature hydrothermal method and characterized by X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and electron microscopy techniques. The Ni3TeO6 phase was found to be an active component in the NTO/eGR composite, which exhibited satisfactory analytical performance in MTBZ detection with a sensitivity of 1.454 µA µM−1 cm−2. Moreover, the NTO-eGR electrode exhibited high selectivity to MTBZ even in the presence of a five-fold excess of interfering species in water and soil samples. The studies on practical applicability revealed that NTO-eGR exhibits good reproducibility with a relative standard deviation of 2.67% (n = 5). Moreover, good recoveries of greater than 90% were achieved in the determination of MTBZ in soil and water samples. Hence, the NTO-eGR sensor electrode is highly suitable for the rapid on-site determination of MTBZ.

Electrochemical Impedance Spectroscopy in the Characterisation and Application of Modified Electrodes for Electrochemical Sensors and Biosensors.

Electrochemical impedance spectroscopy is finding increasing use in electrochemical sensors and biosensors, both in their characterisation, including during successive phases of sensor construction, and in application as a quantitative determination technique. Much of the published work continues to make little use of all the information that can be furnished by full physical modelling and analysis of the impedance spectra, and thus does not throw more than a superficial light on the processes occurring. Analysis is often restricted to estimating values of charge transfer resistances without interpretation and ignoring other electrical equivalent circuit components. In this article, the important basics of electrochemical impedance for electrochemical sensors and biosensors are presented, focussing on the necessary electrical circuit elements. This is followed by examples of its use in characterisation and in electroanalytical applications, at the same time demonstrating how fuller use can be made of the information obtained from complete modelling and analysis of the data in the spectra, the values of the circuit components and their physical meaning. The future outlook for electrochemical impedance in the sensing field is discussed.

Rapid fabrication of partially exfoliated graphite foil with 3D hierarchical structure and its application in electrochemical detection of olaquindox

An ultra-fast and controllable electrochemical method for preparation of partially exfoliated graphite foil (EG) was developed. The exfoliation process was realized in NaH 2 PO 4 aqueous by a multi potential step (STEP) technology. The EG electrode possesses a 3D hierarchical structure with the graphene-like nanosheets seamlessly linked with the graphite substrate. The EG electrode can be directly used as working electrode of electrochemical sensor. The performed sensor displayed high performance for electrochemical detection of olaquindox (OLA), with limit of detection (LOD) of 15 nM and a linear range of 0.05–18.0 μM. In this work, an ultra-fast (150 s) and controllable electrochemical method for preparation of partially exfoliated graphite foil (EG) was developed. The exfoliation process was realized in NaH 2 PO 4 aqueous by a multi potential step (STEP) technology. The graphite foil (G) was switched periodically between the mild oxidation potential and the reduction potential, avoiding completely exfoliation of the graphene sheets. The exfoliation degree of G electrode can be strictly controlled by the electrochemical parameters. The EG electrode possesses a hierarchical structure with the graphene-like nanosheets seamlessly linked with the graphite substrate, which can ensure its direct use as working electrodes for electrochemical sensors. Owing to its large surface area (6.1 times higher than G electrode), extremely low resistance ( R ct = 0.247 Ω), and promotion of adsorption of molecules, the EG electrode displayed surpassed catalytic effect toward the electrochemical redox of olaquindox (OLA). Based on the EG electrode, a high-performance electrochemical sensor for detection of OLA was constructed. The limit of detection (LOD) of the proposed sensor was achieved to be 15 nM (S/N=3), with a linear range of 0.05 – 18.0 μM and ultra-high sensitivity of 2.94 × 10 −3 A cm −2 μM −1 . Further, the low cost, flexible, small volume and easy integration make the EG electrode based sensor promise perspectives in the field of electroanalysis.

Tetracyanonickelate (II)/KOH/reduced graphene oxide fabricated carbon felt for mediated electron transfer type electrochemical sensor for efficient detection of N2O gas at room temperature

N2O is the most significant anthropogenic greenhouse gas, which cause the ozone depletion. Hence, the room temperature detection of N2O is highly desirable. In this work, The TCN(II)–KOH-rGO/CF modified electrode was successfully fabricated by drop coating method. The synthesized electrode was successfully characterized by SEM, TEM, FT-IR and XRD. The sensor electrode was used to detect different N2O concentration in flow conditions at room temperature. TCN(II)–KOH-rGO/CF modified electrode showed high sensitivity towards N2O, a wide range from 1ppm to 16 ppm and low detection of 1 ppm N2O were achieved for the TCN(II)–KOH-rGO/CF modified electrode. The limit of detection and the response towards this nitrogen oxide is competitive to other sensing methods. In addition, the sensitivity of the electrochemical sensor electrode was compared with the online Gas Chromatography. Additionally, the selectivity of the working electrode was analyzed and specified. The working electrode stability was analyzed for more than 30 days shows good stability. The fabricated TCN(II)–KOH-rGO/CF electrode is easier to prepare to get excellent analytical performance towards N2O. Hence, the proposed TCN(II)–KOH-rGO/CF electrode could be the suitable material for detection of N2O in the real site process.