Fabrication of Silver Nanoparticle Loaded into Nanocellulose Derived from Hemp and Poly(vinyl alcohol)-Based Composite as an Electrode for Electrochemical Sensors for Lactate Determination.

Abstract

Nanocellulose derived from hemp (HNC) with the addition of silver nanoparticles (AgNPs) is utilized for improving the electrochemical sensing performances for lactate detection. Initially, HNC is chemically extracted and purified by using alkali treatment and acid hydrolysis. Then, AgNPs are nucleated in situ by the self-reduction process prior to forming a composite with poly(vinyl alcohol) (PVA). This nanocomposite significantly improves the electrochemical properties of the electrode, including electrochemical conductivity and electrocatalysis. The morphologies and chemical alterations of the HNC/AgNPs-PVA nanocomposite are investigated by field emission scanning electron microscopy. It demonstrates a three-dimensional network with random orientation of the nanocellulose fiber. The AgNPs are well-dispersed in the nanocomposite. Moreover, the nanocomposite provides high thermal stability up to 450 °C. Then, it is remarkably noted that 10 wt % HNC/AgNPs-PVA modified on the electrode provides the highest current responses, with a standard redox couple [(Fe(CN)6]3-/4-]. For lactate detection, this modified screen-printed graphene electrode with nonimmobilized lactate oxidase exhibits an increase in the current signal with the increment of lactate concentration and offered a linear range of 0-25 mM, covering a cutoff value (12.5 mM) for muscle fatigue indication. Eventually, this sensor is successfully applied for lactate detection with high potential for a wearable lactate sensor.