Electrodermal Activity Responses Research Articles

Neurophysiological markers of hedonic taste assessment

In recent years, there has been significant interest in studying hedonistic food experiences using neurophysiological methods within the field of neuroscience. Understanding the neural mechanisms behind the perception of pleasure and aversion to food stimuli has implications for both the fundamental processes of human sensory and emotional taste processing and for addressing health-related issues such as obesity and eating disorders.In our study, we used functional near-infrared spectroscopy (fNIRS), heart rate (HR), electrodermal activity (EDA), and electromyography (EMG) to measure brain and peripheral nervous system reactions to pleasant and neutral foods.Using fNIRS, we found that while the insula was activated in response to subjectively pleasant food, the right precentral gyrus was activated in response to neutral taste. This finding suggests interhemispheric asymmetry in relation to neutral taste. The EMG results demonstrated that the corrugator facial muscle was sensitive to neutral food, whereas the zygomaticus facial muscle was sensitive to pleasant food. EDA responses were larger for pleasant food, and HR increased in response to all food stimuli.The convergence of neuroimaging and peripheral physiological results provides a powerful framework for advancing our understanding of hedonistic responses to food. By elucidating the neural and physiological bases of food hedonics, researchers can gain insights into the complex nature of human appetite.

Mild hunger elicits attentional desensitization to visual food cues in healthy, non-obese individuals.

Food is a vital human need, and the human visual system is finely tuned to detect and respond to food cues in the environment. The omnipresence of food cues across various settings has been linked to the prevalence of obesity in susceptible populations. However, the influence of the post-prandial state on visual attention to food stimuli remains poorly understood. This study aimed to elucidate how a 12 hour fast affects visual attention to food and non-food stimuli in healthy, non-obese individuals. Visual attention was assessed by measuring the total duration of visual fixations on stimuli presented on a computer screen, using a screen-based eye tracker (Tobii X2-60). Participants were divided into two groups: those who had fasted for 12 hours and those tested within two hours after consuming breakfast (satiated state). Additionally, performance on the Food Stroop task and electrodermal activity (EDA) responses were measured to evaluate attentional interference and physiological arousal, respectively. Salivary samples were also collected to assess levels of alpha-amylase and cortisol. Fasted participants exhibited a progressive decline in visual attention toward food stimuli compared to satiated individuals, reflecting a satiated state. This effect was independent of the palatability of the depicted food items and was not observed with stimuli representing non-food items. The Food Stroop task revealed no differences between fasting and satiated participants, indicating that the presence of food-related stimuli does not differentially impact attentional interference under varying hunger states. Moreover, no significant variations were observed in EDA responses across participant groups and stimulus types, suggesting that the modulation of visual attention to food cues by hunger is independent of physiological arousal. Interestingly, satiated subjects exhibited higher levels of salivary alpha-amylase, which was inversely related to their subjective hunger ratings. No differences in salivary cortisol levels were found between groups. The findings indicate a novel influence of mild hunger on the processing of visual food cues, independent of physiological arousal. The decline in visual attention to food stimuli in fasted individuals suggests that satiety modulates visual processing. The lack of differences in attentional interference and physiological arousal between fasting and satiated states further supports the notion that visual attention to food cues is primarily driven by hunger-related mechanisms rather than stress. Additionally, the inverse relationship between salivary alpha-amylase levels and hunger ratings implies that alpha-amylase may serve as a marker of satiety rather than stress.

Disturbances in Electrodermal Activity Recordings Due to Different Noises in the Environment.

Electrodermal activity (EDA) is a widely used psychophysiological measurement in laboratory-based studies. In recent times, these measurements have seen a transfer from the laboratory to wearable devices due to the simplicity of EDA measurement as well as modern electronics. However, proper conditions for EDA measurement are recommended once wearable devices are used, and the ambient conditions may influence such measurements. It is not completely known how different types of ambient noise impact EDA measurement and how this translates to wearable EDA measurement. Therefore, this study explored the effects of various noise disturbances on the generation of EDA responses using a system for the simultaneous recording of all measures of EDA, i.e., skin conductance responses (SCRs), skin susceptance responses (SSRs), and skin potential responses (SPRs), at the same skin site. The SCRs, SSRs, and SPRs due to five types of noise stimuli at different sound pressure levels (70, 75, 80, 85, and 90 dB) were measured from 40 participants. The obtained results showed that EDA responses were generated at all levels and that the EDA response magnitudes were significantly (p < 0.001) influenced by the increasing noise levels. Different types of environmental noise may elicit EDA responses and influence wearable recordings outside the laboratory, where such noises are more likely than in standardized laboratory tests. Depending on the application, it is recommended to prevent these types of unwanted variation, presenting a challenge for the quality of wearable EDA measurement in real-world conditions. Future developments to shorten the quality gap between standardized laboratory-based and wearable EDA measurements may include adding microphone sensors and algorithms to detect, classify, and process the noise-related EDA.

Feasibility of using electrodermal activity responses to assess level of crowdedness of pedestrian paths

As urban populations grow, it's imperative to evaluate and enhance the quality of pedestrian paths from the user's perspective. Crowdedness, associated with discomfort and safety, is crucial in determining the overall walking quality and user experience. Previously utilized methods for measuring crowdedness, such as travel diaries and floating population surveys, were limited to collecting perceptual data from sporadic surveys with restricted spatial coverage. Similarly, methods based on CCTV or mobile service data have been used but present issues with blind spots and fail to consider pedestrian perspectives. Against this background, this study explores the feasibility of assessing crowdedness levels by measuring subjects' physiological responses in a laboratory setting based on visual images of real and virtual environments. This study hypothesizes that the amount of people or vehicles passing by affects the electrodermal activity (EDA) of pedestrians, indicating the comfort level of using the environment. Experimental EDA data were measured using a wearable device while the subjects were watching videos showing different pedestrian traffic flows. Representative EDA signal features (e.g., skin conductance responses) were extracted after data pre-processing. Noticeable changes in EDA responses are observed when subjects countered specific environmental variations, such as differing volumes of passing people, on pedestrian paths. The findings suggest that EDA data can be instrumental in differentiating crowdedness levels on pedestrian paths. This study contributes to the body of knowledge by demonstrating the potential of EDA data to characterize the crowdedness experienced by pedestrians. This aids in the development of a novel, quantitative method to gauge pedestrian path crowdedness and to discern contributing factors, such as path width.

Viewers’ sensations: Using skin sensor technology to assess wearable technology

Wearable technology garments have been displayed for aesthetic evaluation in museums. With the additional multisensory cues in wearable technology garments such as digital sounds and LED lights, this study examined 44 museum visitors’ electrodermal activity (EDA) sensory responses to a wearable technology garment in a museum. Results support that apparel with music-only sensory cues (rather than lights-only, no music or lights and both music along with lights) created an enhanced sensory response due to viewers of the music-only garment having the highest EDA response after accounting for their baseline EDA. It is suggested that apparel designers and museum curators consider incorporating music and even music with lights into their designs for display at museums to promote enhanced sensory engagement for viewers. Conversely, it is not recommended that designs utilize lights-only in these designs without the presence of music.

The impact of different types and levels of construction noise on physiological responses: Focusing on standardization and habituation

Construction noise is a pervasive and influential source of stress for urban residents and can lead to various health problems, including hormonal dysfunction and cardiovascular diseases. Despite considerable research on the general impact of urban noise, studies that specifically address physiological responses to construction noise are limited. This study explores the relationship between different types of construction noise (tonal and impulsive) and physiological responses, focusing on electrodermal activity (EDA) at varying decibel levels (40, 60, and 80 dBA). The experimental results demonstrated that the z-score standardization method excels in EDA analysis in response to construction noise, particularly showing that noise levels significantly affect the phasic EDA of subjects exposed to tonal noise. Additionally, it was found that EDA responses recorded during the initial 30 s decisively influenced the subjective rating of annoyance. These results highlight the importance of considering both noise type and exposure duration in managing construction noise to mitigate its negative impacts on urban residents. This study can provide practical implications for urban planning, potentially minimizing complaints from urban residents and the impact of construction noise on communities.

Increased stress burden and electrodermal reactivity in bladder cancer patients in comparison to healthy controls

The purpose of our study was to assess specific physiological parameters associated with stress responses in bladder cancer (BCa) patients compared to healthy individuals. By examining the transition from a supine to a sitting position, representing a mild physiological load, we investigated the changes in autonomic nervous system (ANS) activity as reflected by alterations in these parameters, indicating shifts in ANS regulation, using non-linear heart rate variability (HRV) parameters (0V%, 2UV%, parasympathetic and sympathetic nervous system – PNS and SNS indices), modified heart rate acceleration (ACmod) and deceleration capacities (DCmod), heart rate (HR), electrodermal activity (EDA), and also their correlations with perceived stress score. Our findings showed that BCa patients (n = 38) exhibited elevated resting HR, heightened SNS index, and increased EDA compared to their healthy counterparts (n = 47), indicating a notable physiological stress burden. The 0V% parameter showed a positive association with the SNS index, ACmod, HR, and EDA parameters, while displaying a negative correlation with the PNS index, DCmod and 2UV%. These non-linear HRV parameters, such as 0V% and 2UV%, offer nuanced insights into the complexities of heartbeat dynamics and autonomic regulation. After the transition from supine to sitting positions, BCa patients displayed higher EDA responses, indicating heightened stress reactivity and ANS sensitivity. These physiological distinctions persisted even when we did not prove differences in the levels of perceived stress between the studied groups. In conclusion, our study emphasizes the significance of identifying cancer patients at risk of ANS dysregulation, paving the way for tailored stress management strategies.

Improvisation and live accompaniment increase motor response and reward during a music playing task

Music provides a reward that can enhance learning and motivation in humans. While music is often combined with exercise to improve performance and upregulate mood, the relationship between music-induced reward and motor output is poorly understood. Here, we study music reward and motor output at the same time by capitalizing on music playing. Specifically, we investigate the effects of music improvisation and live accompaniment on motor, autonomic, and affective responses. Thirty adults performed a drumming task while (i) improvising or maintaining the beat and (ii) with live or recorded accompaniment. Motor response was characterized by acceleration of hand movements (accelerometry), wrist flexor and extensor muscle activation (electromyography), and the drum strike count (i.e., the number of drum strikes played). Autonomic arousal was measured by tonic response of electrodermal activity (EDA) and heart rate (HR). Affective responses were measured by a 12-item Likert scale. The combination of improvisation and live accompaniment, as compared to all other conditions, significantly increased acceleration of hand movements and muscle activation, as well as participant reports of reward during music playing. Improvisation, regardless of type of accompaniment, increased the drum strike count and autonomic arousal (including tonic EDA responses and several measures of HR), as well as participant reports of challenge. Importantly, increased motor response was associated with increased reward ratings during music improvisation, but not while participants were maintaining the beat. The increased motor responses achieved with improvisation and live accompaniment have important implications for enhancing dose of movement during exercise and physical rehabilitation.

Zooming into the Complex Dynamics of Electrodermal Activity Recorded during Emotional Stimuli: A Multiscale Approach.

Physiological phenomena exhibit complex behaviours arising at multiple time scales. To investigate them, techniques derived from chaos theory were applied to physiological signals, providing promising results in distinguishing between healthy and pathological states. Fractal-like properties of electrodermal activity (EDA), a well-validated tool for monitoring the autonomic nervous system state, have been reported in previous literature. This study proposes the multiscale complexity index of electrodermal activity (MComEDA) to discern different autonomic responses based on EDA signals. This method builds upon our previously proposed algorithm, ComEDA, and it is empowered with a coarse-graining procedure to provide a view at multiple time scales of the EDA response. We tested MComEDA's performance on the EDA signals of two publicly available datasets, i.e., the Continuously Annotated Signals of Emotion (CASE) dataset and the Affect, Personality and Mood Research on Individuals and Groups (AMIGOS) dataset, both containing physiological data recorded from healthy participants during the view of ultra-short emotional video clips. Our results highlighted that the values of MComEDA were significantly different (p-value < 0.05 after Wilcoxon signed rank test with Bonferroni's correction) when comparing high- and low-arousal stimuli. Furthermore, MComEDA outperformed the single-scale approach in discriminating among different valence levels of high-arousal stimuli, e.g., showing significantly different values for scary and amusing stimuli (p-value = 0.024). These findings suggest that a multiscale approach to the nonlinear analysis of EDA signals can improve the information gathered on task-specific autonomic response, even when ultra-short time series are considered.

Comparisons of Evoked Skin Vasomotor and Sudomotor Responses to Single Pulse Median Nerve Stimulation

The sensorimotor dysfunction caused by cervical and thoracic spinal cord injury (SCI) is concomitant with sympathetic nervous system dysfunction, evidenced by the high prevalence of orthostatic hypotension and autonomic dysreflexia. Although the International Standards for Neurological Classification of SCI evaluate sensorimotor function both above and below the injury level, there are limited quantitative measures for gauging sympathetic outflow and the extent of dysautonomia resulting from neurological damage. Despite studies on the effects of SCI on electrodermal activity (EDA) and blood pressure (BP), there is a lack of detailed feature comparisons involving EDA, skin vasomotor activity, and BP. Therefore, we investigated the relationship between EDA, skin vasomotor, and BP responses to sympathetic activation via median nerve stimulation in persons with and without SCI. Adults with and without SCI (cohort demographics presented in poster) participated in this study. Lead II electrocardiogram (ECG), pneumogram (P), photoplethysmogram (PPG), and EDA were recorded from either the trunk (ECG, P) or both limbs (PPG, EDA). Beat-by-beat BP was recorded from the middle finger of the left hand (VitalStream, CareTaker Medical). Evoked responses in the above signals were measured surrounding a cathodal electrical pulse (30 mA, 200 μs) delivered to the distal right median nerve 1-2 cm proximal and medial to the radial styloid process either during systole or diastole of the cardiac cycle. There were notable differences between baseline signals before and after the experimental session, along with a consistent reduction of limb temperature (&gt; 2° Celsius). Increases in EDA (greater than two standard deviations from baseline) were found across non-SCI participants following stimulation both during systole and diastole, along with skin vasoconstriction following the onset of EDA modulation (&gt; 50% of trials). Individual differences were noted in evoked responses to stimulation at systole vs. diastole. Furthermore, attenuated responses were observed in individuals with SCI. Stimulation caused no evoked signal response habituation across trials within participants. Additionally, evoked BP changes were not consistent across all individuals with respect to stimulation. Our results show a fixed relationship between evoked skin vasomotor and sudomotor responses to single pulse stimuli across participants and had consistent waveform features. Potential individual effects of stimulation delivered at systole vs. diastole warrant an in-depth analysis of reflex gating of sudomotor and skin vasomotor responses. This would present insight into the population with SCI, where dysfunction could greatly exaggerate reflex gating. 1R01NS131493-01. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Changes in electrodermal activity following sympathicotomy in hyperhidrosis patients.

The aim of this study was to assess the potential of electrodermal activity (EDA) as a diagnostic tool for preoperative evaluation in hyperhidrosis patients. EDA levels and patterns in different skin areas were investigated before and after endoscopic thoracic sympathicotomy (ETS) and was compared to healthy subjects. Thirty-seven patients underwent two days of measurements before and after the operation. Twenty-five (67.5%) of the patients also had a third measurement after six months. Non-invasive EDA measurements, involving skin conductance, were sampled from five different skin areas while patients were at rest in supine and sitting positions or when subjected to stimuli such as deep inspirations, mental challenge, and exposure to a sudden loud sound. Prior to the operation, hyperhidrosis patients showed higher spontaneous palm EDA variations at rest and stronger responses to stimuli compared to healthy subjects. Patients with facial blushing/hyperhidrosis or combined facial/palmar hyperhidrosis showed minimal spontaneous activity or responses, particularly during mental challenge and sound stimulus. Notably, palm EDA response was abolished shortly following sympathicotomy, although a minor response was observed after six months. Minimal EDA responses were also observed in the back and abdomen postoperatively. Hyperhidrosis patients showed stronger EDA response to stimuli compared to healthy subjects. Sympathicotomy resulted in the complete elimination of palm EDA responses, gradually returning to a limited extent after six months. These findings suggest that EDA recordings could be utilized in preoperative assessment of hyperhidrosis patients.

VARIATIONS IN FREQUENCY AND AMPLITUDE OF NONSPECIFIC ELECTRODERMAL RESPONSES

VARIATIONS IN FREQUENCY AND AMPLITUDE OF NONSPECIFIC ELECTRODERMAL RESPONSES

Sensing walkability? Opportunities and limitations of electrodermal activity as an indicator of walkable urban environments

ABSTRACT Stress and other emotions are increasingly used as a lens for evaluating the quality of urban environments. Researchers hope that wearable biosensors may allow for widespread measurement of emotions during everyday activities. There are substantial technical and theoretical challenges to deploying biosensors in field settings and meaningfully interpreting their data. This study evaluates how electrodermal activity (EDA) may be used to understand walkers’ responses to environmental design along real-world streets. Results show that subjects have consistent EDA responses to some environmental variables, but that EDA does not straightforwardly indicate emotional valence–favor or disfavor–potentially limiting its usefulness for planning.

Impairment of unconscious emotional processing after unilateral medial temporal structure resection

The role of the amygdala in unconscious emotional processing remains a topic of debate. Past lesion studies have indicated that amygdala damage leads to impaired electrodermal activity in response to subliminally presented emotional stimuli. However, electrodermal activity can reflect both emotional and nonemotional processes. To provide behavioral evidence highlighting the critical role of the amygdala in unconscious emotional processing, we examined patients (n = 16) who had undergone unilateral resection of medial temporal lobe structures, including the amygdala. We utilized the subliminal affective priming paradigm in conjunction with unilateral visual presentation. Fearful or happy dynamic facial expressions were presented in unilateral visual fields for 30 ms, serving as negative or positive primes. Subsequently, neutral target faces were displayed, and participants were tasked with rating the valence of these targets. Positive primes, compared to negative ones, enhanced valence ratings of the target to a greater extent when they stimulated the intact hemisphere (i.e., were presented in the contralateral visual field of the intact hemisphere) than when they stimulated the resected hemisphere (i.e., were presented in the contralateral visual field of the resected hemisphere). These results suggest that the amygdala is causally involved in unconscious emotional processing.

Combining electrodermal activity analysis and dynamic causal modeling to investigate the visual-odor multimodal integration during face perception

Objective. This study presents a novel methodological approach for incorporating information related to the peripheral sympathetic response into the investigation of neural dynamics. Particularly, we explore how hedonic contextual olfactory stimuli influence the processing of neutral faces in terms of sympathetic response, event-related potentials and effective connectivity analysis. The objective is to investigate how the emotional valence of odors influences the cortical connectivity underlying face processing and the role of face-induced sympathetic arousal in this visual-olfactory multimodal integration. Approach. To this aim, we combine electrodermal activity (EDA) analysis and dynamic causal modeling to examine changes in cortico-cortical interactions. Results. The results reveal that stimuli arising sympathetic EDA responses are associated with a more negative N170 amplitude, which may be a marker of heightened arousal in response to faces. Hedonic odors, on the other hand, lead to a more negative N1 component and a reduced the vertex positive potential when they are unpleasant or pleasant. Concerning connectivity, unpleasant odors strengthen the forward connection from the inferior temporal gyrus (ITG) to the middle temporal gyrus, which is involved in processing changeable facial features. Conversely, the occurrence of sympathetic responses after a stimulus is correlated with an inhibition of this same connection and an enhancement of the backward connection from ITG to the fusiform face gyrus. Significance. These findings suggest that unpleasant odors may enhance the interpretation of emotional expressions and mental states, while faces capable of eliciting sympathetic arousal prioritize identity processing.

Utilising electrodermal activity sensor signals to quantify nociceptive response during movement activities

ObjectiveWith an increasingly ageing population and osteoarthritis prevalence, the quantification of nociceptive signals responsible for painful movements and individual responses could lead to better treatment and monitoring solutions. Changes in electrodermal activity (EDA) can be detected via changes in skin conductance (SC) and measured using finger electrodes on a wearable sensor, providing objective information for increased physiological stress response.ResultsTo provide EDA response preliminary data, this was recorded with healthy volunteers on an array of activities while receiving a noxious stimulus. This provides a defined scenario that can be utilised as protocol feasibility testing. Raw signal extraction, processing and statistical analysis was performed using mean SC values on all participant data. The application of the stimuli resulted in a significant average increase (p < 0.05) in mean SC in four out of five activities with significant gender differences (p < 0.05) in SC and self-reported pain scores and large effect sizes. Though EDA parameters are a promising tool for nociceptive response indicators, limitations including motion artifact sensitivities and lack of previous movement-based EDA published data result in restricted analysis understanding. Refined processing pipelines with signal decomposition tools could be utilised in a protocol that quantifies nociceptive response clinically meaningfully.

The Five Basic Human Senses Evoke Electrodermal Activity.

Electrodermal activity (EDA) usually relates to variations in the electrical properties of palmar or plantar skin sites. EDA responses, namely skin conductance responses (SCRs), skin potential responses (SPRs) and skin susceptance responses (SSRs) are shown to be sensitive indexes of sympathetic nervous system activation and are studied in many research projects. However, the association between EDA responses and the five basic human senses has not been investigated yet. Our study aimed to explore the relationship between the three EDA responses (SCRs, SSRs and SPRs) and the five basic human senses. These three EDA responses were measured simultaneously at the same skin site on each of the 38 volunteers. The tested five senses were sight, hearing, touch, taste and smell. The results showed that the different tested senses led to different degrees of EDA responses due to activation of the sympathetic nervous system and corresponding secretion of sweat. Although a controlled study on the degree of EDA as a function of the strength of each stimulus was not performed, we noted that the largest EDA responses were typically associated with the smell sense test. We conclude that EDA responses could be utilized as measures for examining the sensitivity of the human senses. Hence, EDA devices may have important roles in sensory systems for future clinical applications.

Differentiating between stress- and EPT-induced electrodermal activity during dental examination

Dental pain invokes the sympathetic nervous system, which can be measured by electrodermal activity (EDA). In the dental clinic, accurate quantification of pain is needed because it could enable optimized drug-dose treatments, thereby potentially reducing drug addiction. However, a confounding factor is that during pain there is also lingering residual stress, hence, both contribute to the EDA response. Therefore, we investigated whether EDA can differentiate stress from pain during dental examination. The use of electrical pulp test (EPT) is an ideal approach to tease out the dynamics of stress and mimic pain with lingering residual stress. Once the electrical sensation is felt and reaches a critical current threshold, the subject removes the probe from their tooth, hence, this stage of data represents largely EPT stimulus and the residual stress-induced EDA response is smaller. EPT was performed on necrotic and vital teeth in fifty-one subjects. We defined four different data groups of reactions based on each individual's EPT intensity level expectation based on the visual analog scale (VAS) of their baseline trial, as follows: mild stress, mild stress + EPT, strong stress, and strong stress + EPT. EDA-derived features exhibited significant difference between residual lingering stress + EPT groups and stress groups. We obtained 84.6% accuracy with 76.2% sensitivity and 86.8% specificity with multilayer perceptron in differentiating between pure-stress groups vs. stress + EPT groups. Moreover, EPT induced much greater EDA amplitude and faster response than stress. Our finding suggests that our machine learning approach can discriminate between stress and EPT stimulation in EDA signals.

Lateralization of autonomic output in response to limb-specific threat.

In times of stress or danger, the autonomic nervous system (ANS) signals the fight or flight response. A canonical function of ANS activity is to globally mobilize metabolic resources, preparing the organism to respond to threat. Yet a body of research has demonstrated that, rather than displaying a homogenous pattern across the body, autonomic responses to arousing events, as measured through changes in electrodermal activity (EDA), can differ between right and left body locations. Surprisingly, an attempt to identify a function of ANS asymmetry consistent with its metabolic role has not been investigated. In the current study, we investigated whether asymmetric autonomic responses could be induced through limb-specific aversive stimulation. Participants were given mild electric stimulation to either the left or right arm while EDA was monitored bilaterally. In a group-level analyses, an ipsilateral EDA response bias was observed, with increased EDA response in the hand adjacent to the stimulation. This effect was observable in ∼50% of individual participants. These results demonstrate that autonomic output is more complex than canonical interpretations suggest. We suggest that, in stressful situations, autonomic outputs can prepare either the whole-body fight or flight response, or a simply a limb-localized flick, which can effectively neutralize the threat while minimizing global resource consumption. These findings are consistent with recent theories proposing evolutionary leveraging of neural structures organized to mediate sensory responses for processing of cognitive emotional cues.

Comparing the Effectiveness of Speech and Physiological Features in Explaining Emotional Responses during Voice User Interface Interactions

The rapid rise of voice user interface technology has changed the way users traditionally interact with interfaces, as tasks requiring gestural or visual attention are swapped by vocal commands. This shift has equally affected designers, required to disregard common digital interface guidelines in order to adapt to non-visual user interaction (No-UI) methods. The guidelines regarding voice user interface evaluation are far from the maturity of those surrounding digital interface evaluation, resulting in a lack of consensus and clarity. Thus, we sought to contribute to the emerging literature regarding voice user interface evaluation and, consequently, assist user experience professionals in their quest to create optimal vocal experiences. To do so, we compared the effectiveness of physiological features (e.g., phasic electrodermal activity amplitude) and speech features (e.g., spectral slope amplitude) to predict the intensity of users’ emotional responses during voice user interface interactions. We performed a within-subjects experiment in which the speech, facial expression, and electrodermal activity responses of 16 participants were recorded during voice user interface interactions that were purposely designed to elicit frustration and shock, resulting in 188 analyzed interactions. Our results suggest that the physiological measure of facial expression and its extracted feature, automatic facial expression-based valence, is most informative of emotional events lived through voice user interface interactions. By comparing the unique effectiveness of each feature, theoretical and practical contributions may be noted, as the results contribute to voice user interface literature while providing key insights favoring efficient voice user interface evaluation.