Electric Organ Research Articles

Electrocommunication in pulse Gymnotiformes: the role of electric organ discharge (EOD) time course in species identification.

Understanding how individuals detect and recognize signals emitted by conspecifics is fundamental to discussions of animal communication. The species pair Gymnotus omarorum and Brachyhypopomus gauderio, found in syntopy in Uruguay, emit species-specific electric organ discharge (EOD) that can be sensed by both species. The aim of this study was to unveil whether either of these species is able to identify a conspecific EOD, and to investigate distinctive recognition signal features. We designed a forced-choice experiment using a natural behavior (i.e. tracking electric field lines towards their source) in which each fish had to choose between a conspecific and a heterospecific electric field. We found a clear pattern of preference for a conspecific waveform even when pulses were played within 1 Hz of the same rate. By manipulating the time course of the explored signals, we found that the signal features for preference between conspecific and heterospecific waveforms were embedded in the time course of the signals. This study provides evidence that pulse Gymnotiformes can recognize a conspecific exclusively through species-specific electrosensory signals. It also suggests that the key signal features for species differentiation are probably encoded by burst coder electroreceptors. Given these results, and because receptors are sharply tuned to amplitude spectra and also tuned to phase spectra, we extend the electric color hypothesis used in the evaluation of objects to apply to communication signals.

Development of a sexual dimorphism in a central pattern generator driving a rhythmic behavior: The role of glia-mediated potassium buffering in the pacemaker nucleus of the weakly electric fish Apteronotus leptorhynchus.

Central pattern generators play a critical role in the neural control of rhythmic behaviors. One of their characteristic features is the ability to modulate the oscillatory output. An important yet little-studied type of modulation involves the generation of oscillations that are sexually dimorphic in frequency. In the weakly electric fish Apteronotus leptorhynchus, the pacemaker nucleus serves as a central pattern generator that drives the electric organ discharge of the fish in a one-to-one fashion. Males discharge at higher frequencies than females-a sexual dimorphism that develops under the influence of steroid hormones. The two principal neurons that constitute the oscillatory network of the pacemaker nucleus are the pacemaker and relay cells. Whereas the number and size of the pacemaker and relay cells are sexually monomorphic, pronounced sex-dependent differences exist in the morphology, and subcellular properties of astrocytes, which form a syncytium closely associated with these neurons. In females, compared to males, the astrocytic syncytium covers a larger area surrounding the pacemaker and relay cells and exhibits higher levels of expression of connexin-43 expression. The latter indicates a strong gap-junction coupling of the individual cells within the syncytium. It is hypothesized that these sex-specific differences result in an increased capacity for buffering of extracellular potassium ions, thereby lowering the potassium equilibrium potential, which, in turn, leads to a decrease in the oscillation frequency. This hypothesis has received strong support from simulations based on computational models of individual neurons and the whole neural network of the pacemaker nucleus.

Tethered unitary recordings suggest a spike-timing electrosensory code in the electrosensory lobe of Gymnotus omarorum

AbstractEvaluation of neural activity during natural behaviours is essential for understanding how the brain works. Here we show that neuron-specific self-evoked firing patterns are modulated by an object’s presence, at the electrosensory lobe neurons of tethered-moving Gymnotus omarorum. This novel preparation shows that electrosensory signals in these pulse-type weakly electric fish are not only encoded in the number of spikes per electric organ discharge (EOD), as is the case in wave-type electric fish, but also in the spike timing pattern after each EOD, as found in pulse-type Mormyroidea. Present data suggest that pulsant electrogenesis and spike timing coding of electrosensory signals developed concomitantly in the same species, and evolved convergently in African and American electric fish.

Tracking activity patterns of a multispecies community of gymnotiform weakly electric fish in their neotropical habitat without tagging.

Field studies on freely behaving animals commonly require tagging and often are focused on single species. Weakly electric fish generate a species- and individual-specific electric organ discharge (EOD) and therefore provide a unique opportunity for individual tracking without tagging. Here, we present and test tracking algorithms based on recordings with submerged electrode arrays. Harmonic structures extracted from power spectra provide fish identity. Localization of fish based on weighted averages of their EOD amplitudes is found to be more robust than fitting a dipole model. We apply these techniques to monitor a community of three species, Apteronotus rostratus, Eigenmannia humboldtii and Sternopygus dariensis, in their natural habitat in Darién, Panama. We found consistent upstream movements after sunset followed by downstream movements in the second half of the night. Extrapolations of these movements and estimates of fish density obtained from additional transect data suggest that some fish cover at least several hundreds of meters of the stream per night. Most fish, including E. humboldtii, were traversing the electrode array solitarily. From in situ measurements of the decay of the EOD amplitude with distance of individual animals, we estimated that fish can detect conspecifics at distances of up to 2 m. Our recordings also emphasize the complexity of natural electrosensory scenes resulting from the interactions of the EODs of different species. Electrode arrays thus provide an unprecedented window into the so-far hidden nocturnal activities of multispecies communities of weakly electric fish at an unmatched level of detail.

Photoinduced Directional Proton Transport through Printed Asymmetric Graphene Oxide Superstructures: A New Driving Mechanism under Full‐Area Light Illumination

Abstract2D‐material‐based membranes with densely packed sub‐nanometer‐height fluidic channels show exceptional transport properties, and have attracted broad research interest for energy‐, environment‐, and healthcare‐related applications. Recently, light‐controlled active transport of ionic species in abiotic materials have received renewed attention. However, its dependence on inhomogeneous or site‐specific illumination is a challenge for scalable application. Here, directional proton transport through printed asymmetric graphene oxide superstructures (GOSs) is demonstrated under full‐area illumination. The GOSs are composed of partially stacked graphene oxide multilayers formed by a two‐step direct ink writing process. The direction of the photoinduced proton current is determined by the position of top graphene oxide multilayers, which functions as a photogate to modulate the horizontal ion transport through the beneath lamellar nanochannels. This transport phenomenon unveils a new driving mechanism that, in asymmetric nanofluidic structures, the decay of local light intensity in depth direction breaks the balance of electric potential distribution in horizontal direction, and thus generates a photoelectric driving force for ion transport. Following this mechanism, the GOSs are developed into photonic ion transistors with three different gating modes. The asymmetrically printed photonic‐ionic devices provide fundamental elements for light‐harvesting nanofluidic circuits, and may find applications for artificial photosynthesis and artificial electric organs.

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish.

Electroreception and electrogenesis have changed in the evolutionary history of vertebrates. There is a striking degree of convergence in these independently derived phenotypes, which share a common genetic architecture. This is perhaps best exemplified by the numerous convergent features of gymnotiforms and mormyrids, two species-rich teleost clades that produce and detect weak electric fields and are called weakly electric fish. In the 50 years since the discovery that weakly electric fish use electricity to sense their surroundings and communicate, a growing community of scientists has gained tremendous insights into evolution of development, systems and circuits neuroscience, cellular physiology, ecology, evolutionary biology, and behavior. More recently, there has been a proliferation of genomic resources for electric fish. Use of these resources has already facilitated important insights with regards to the connection between genotype and phenotype in these species. A major obstacle to integrating genomics data with phenotypic data of weakly electric fish is a present lack of functional genomics tools. We report here a full protocol for performing CRISPR/Cas9 mutagenesis that utilizes endogenous DNA repair mechanisms in weakly electric fish. We demonstrate that this protocol is equally effective in both the mormyrid species Brienomyrus brachyistius and the gymnotiform Brachyhypopomus gauderio by using CRISPR/Cas9 to target indels and point mutations in the first exon of the sodium channel gene scn4aa. Using this protocol, embryos from both species were obtained and genotyped to confirm that the predicted mutations in the first exon of the sodium channel scn4aa were present. The knock-out success phenotype was confirmed with recordings showing reduced electric organ discharge amplitudes when compared to uninjected size-matched controls.

Toxic and essential metals in Narcine brasiliensis (Elasmobranchii: Narcinidae): A baseline ecotoxicological study in the Southeast Atlantic and preliminary maternal transfer implications

Abstract The electric ray Narcine brasiliensis is captured as by-catch along the Brazilian coast and is considered data deficient by the International Union for Conservation of Nature (IUCN). In the present study, toxic and essential metals in adult and embryonic N. brasiliensis specimens from Southeastern Brazil were assessed by ICP-MS. The possibility of maternal metal transfer to embryos was evaluated. Several toxic elements were detected, including in the electric organ and gonads, leading to potential behavioral and reproductive concerns. Extremely high As levels were detected, probably a direct effect of the Mariana dam rupture ocurred in 2015, where mine tailings were transported throughout the Doce River basin in Southeastern Brazil, contaminating the entire water body. Statistically significant correlations was observed for both Mn and Ag between Size Class 2 embryos and mother tissues, evidencing maternal transfer for these elements. Although no other significant associations were observed, probably due to the low sample number, several toxic metals were also detected in the assessed embryos. This baseline data will aid in future biomonitoring efforts and conservation measures.

The effect of normoxia exposure on hypoxia tolerance and sensory sampling in a swamp-dwelling mormyrid fish

Effects of energetic limitations on the performance of sensory systems are generally difficult to quantify. Weakly electric fishes provide an ideal model system to quantify the effects of metabolic stressors on sensory information acquisition, because they use an active-sensing strategy that permits easy measurement of the sensing effort. These fishes discharge an electric signal and sense perturbations of the resulting electric field. We used the mormyrid Petrocephalus degeni to quantify the relationship between routine metabolic rate and the rate of sensory sampling (rate of electric organ discharge, EOD) while under progressive hypoxia by quantifying the critical oxygen tension (PC-MR) and the critical electric organ discharge threshold (PC-EOD). PC-MR was significantly higher in fish acclimated to normoxia for over 40 days compared to animals tested within 1–5 days of capture from a hypoxic swamp, which suggests high costs of maintaining hypoxia tolerance; however, there was no acclimation effect on PC-EOD. All P. degeni reached their PC-EOD prior to their PC-MR. However, below the respective critical tension value, EOD rate decreased more gradually than the metabolic rate suggesting that the fish were increasing the proportion of their energy budget allocated to acquiring sensory information as dissolved-oxygen levels dropped. Trade-offs between sensory sampling and other physiological functions are also suggested by the increase in routine EOD rate with long-term normoxia acclimation, in contrast to metabolic rate, which showed no significant changes. These results highlight the relationship between sensory sampling and metabolic rate in response to progressive hypoxia and the plasticity of hypoxia tolerance.

A JAR of Chirps: The Gymnotiform Chirp Can Function as Both a Communication Signal and a Jamming Avoidance Response.

The weakly electric gymnotiform fish produce a rhythmic electric organ discharge (EOD) used for communication and active electrolocation. The EOD frequency is entrained to a medullary pacemaker nucleus. During communication and exploration, this rate can be modulated by a pre-pacemaker network, resulting in specific patterns of rate modulation, including stereotyped communication signals and dynamic interactions with conspecifics known as a Jamming Avoidance Response (JAR). One well-known stereotyped signal is the chirp, a brief upward frequency sweep usually lasting less than 500 ms. The abrupt change in frequency has dramatic effects on phase precession between two signalers. We report here on chirping in Brachyhypopmus cf. sullivani, Microsternarchus cf. bilineatus Lineage C, and Steatogenys cf. elegans during conspecific playback experiments. Microsternarchus also exhibits two behaviors that include chirp-like extreme frequency modulations, EOD interruptions with hushing silence and tumultuous rises, and these are described in terms of receiver impact. These behaviors all have substantial impact on interference caused by conspecifics and may be a component of the JAR in some species. Chirps are widely used in electronic communications systems, sonar, and other man-made active sensing systems. The brevity of the chirp, and the phase disruption it causes, makes chirps effective as attention-grabbing or readiness signals. This conforms to the varied assigned functions across gymnotiforms, including pre-combat aggressive or submissive signals or during courtship and mating. The specific behavioral contexts of chirp expression vary across species, but the physical structure of the chirp makes it extremely salient to conspecifics. Chirps may be expected in a wide range of behavioral contexts where their function depends on being noticeable and salient. Further, in pulse gymnotiforms, the chirp is well structured to comprise a robust jamming signal to a conspecific receiver if specifically timed to the receiver’s EOD cycle. Microsternarchus and Steatogenys exploit this feature and include chirps in dynamic jamming avoidance behaviors. This may be an evolutionary re-use of a circuitry for a specific signal in another context.

An Aquatic Wireless Biosensor for Electric Organ Discharge With an Integrated Analog Front End

This paper presents a novel wireless underwater data acquisition system for sensing electric organ discharge (EOD) signals generated from the weakly electric fish S. macrurus . Variation in frequency and amplitude of the EOD signals are of interest by biologists to study behavioral and environmental effects on electric organ cells. In order to record the EOD signals, a miniature wearable wireless sensing system is designed for the target fish. The system consists of a custom-designed integrated analog front-end (AFE), an ATmega328p microcontroller unit and a wireless transmitter (TX), as well as a battery with a power management module. In order to save TX power, the wireless sensor only transmits the calculated frequency and amplitude information of the EOD signal based on a proposed gain-feedback control method. A receiver attached to a host computer receives the wirelessly transmitted EOD data to perform further analysis. A wearable waterproof backpack for the fish is designed to house the wireless sensor and battery. The overall system has been successfully tested in a clinical experiment with the weakly electric fish. The AFE integrated circuit, which includes a novel rail-to-rail dynamic comparator, is fabricated in a 0.18- $\mu \text{m}$ CMOS process. The total area of the core circuit is 1.0 mm2. The LNPA achieved a noise efficiency factor of 1.8. The AFE power consumption is 2.2 $\mu \text{W}$ from a single +1.8 V supply, whereas the overall system power consumption is 15 mW. The proposed gain-feedback control structure for frequency and amplitude measurement saves the transmitter data rate by 3,756 times compared with sending the raw data.

GHOST: Recovering Historical Signal from Heterotachously Evolved Sequence Alignments.

Molecular sequence data that have evolved under the influence of heterotachous evolutionary processes are known to mislead phylogenetic inference. We introduce the General Heterogeneous evolution On a Single Topology (GHOST) model of sequence evolution, implemented under a maximum-likelihood framework in the phylogenetic program IQ-TREE (http://www.iqtree.org). Simulations show that using the GHOST model, IQ-TREE can accurately recover the tree topology, branch lengths, and substitution model parameters from heterotachously evolved sequences. We investigate the performance of the GHOST model on empirical data by sampling phylogenomic alignments of varying lengths from a plastome alignment. We then carry out inference under the GHOST model on a phylogenomic data set composed of 248 genes from 16 taxa, where we find the GHOST model concurs with the currently accepted view, placing turtles as a sister lineage of archosaurs, in contrast to results obtained using traditional variable rates-across-sites models. Finally, we apply the model to a data set composed of a sodium channel gene of 11 fish taxa, finding that the GHOST model is able to elucidate a subtle component of the historical signal, linked to the previously established convergent evolution of the electric organ in two geographically distinct lineages of electric fish. We compare inference under the GHOST model to partitioning by codon position and show that, owing to the minimization of model constraints, the GHOST model offers unique biological insights when applied to empirical data.

Light-Driven Active Proton Transport through Photoacid- and Photobase-Doped Janus Graphene Oxide Membranes.

Biological electrogenic systems use protein-based ionic pumps to move salt ions uphill across a cell membrane to accumulate an ion concentration gradient from the equilibrium physiological environment. Toward high-performance and robust artificial electric organs, attaining an antigradient ion transport mode by fully abiotic materials remains a great challenge. Herein, a light-driven proton pump transport phenomenon through a Janus graphene oxide membrane (JGOM) is reported. The JGOM is fabricated by sequential deposition of graphene oxide (GO) nanosheets modified with photobase (BOH) and photoacid (HA) molecules. Upon ultraviolet light illumination, the generation of a net protonic photocurrent through the JGOM, from the HA-GO to the BOH-GO side, is observed. The directional proton flow can thus establish a transmembrane proton concentration gradient of up to 0.8 pH units mm-2 membrane area at a proton transport rate of 3.0 mol h-1 m-2 . Against a concentration gradient, antigradient proton transport can be achieved. The working principle is explained in terms of asymmetric surface charge polarization on HA-GO and BOH-GO multilayers triggered by photoisomerization reactions, and the consequent intramembrane proton concentration gradient. The implementation of membrane-scale light-harvesting 2D nanofluidic system that mimics the charge process of the bioelectric organs makes a straightforward step toward artificial electrogenic and photosynthetic applications.

Comparative Transcriptome Analyses Reveal the Role of Conserved Function in Electric Organ Convergence Across Electric Fishes.

The independent origins of multiple electric organs (EOs) of fish are fascinating examples of convergent evolution. However, comparative transcriptomics of different electric fish lineages are scarce. In this study, we found that the gene expression of EOs and skeletal muscles from three lineages (Mormyroidea, Siluriformes, and Gymnotiformes) tended to cluster together based on the species of origin, irrespective of the organ from which they are derived. A pairwise comparison of differentially expressed genes (DEGs) revealed that no less than half of shared DEGs exhibited parallel expression differentiation, indicating conserved directionality of differential expression either in or between lineages, but only a few shared DEGs were identified across all focal species. Nevertheless, the functional enrichment analysis of DEGs indicated that there were more parallel gene expression changes at the level of pathways and biological functions. Therefore, we may conclude that there is no parallel evolution of the entire transcriptomes of EOs among different lineages. Further, our results support the hypothesis that it is not different genes but conserved biological functions that play a crucial role in the convergence of complex phenotypes. This study provides insight into the genetic basis underlying the EO convergent evolution; however, more studies in different cases will be needed to demonstrate whether this pattern can be extended to other cases to derive a general rule for convergent evolution.

The Astonishing Behavior of Electric Eels.

The remarkable physiology of the electric eel (Electrophorus electricus) made it one of the first model species in science. It was pivotal for understanding animal electricity in the 1700s, was investigated by Humboldt and Faraday in the 1800s, was leveraged to isolate the acetylcholine receptor in the 20th century, and has inspired the design of new power sources and provided insights to electric organ evolution in the 21st century. And yet few studies have investigated the electric eel’s behavior. This review focuses on a series of recently discovered behaviors that evolved alongside the eel’s extreme physiology. Eels use their high-voltage electric discharge to remotely control prey by transcutaneously activating motor neurons. Hunting eels use this behavior in two different ways. When prey have been detected, eels use high-voltage to cause immobility by inducing sustained, involuntary muscle contractions. On the other hand, when prey are hidden, eels often use brief pulses to induce prey twitch, which causes a water movement detected by the eel’s mechanoreceptors. Once grasped in the eel’s jaws, difficult prey are often subdued by sandwiching them between the two poles (head and tail) of the eel’s powerful electric organ. The resulting concentration of the high-voltage discharge, delivered at high-rates, causes involuntary fatigue in prey muscles. This novel strategy for inactivating muscles is functionally analogous to poisoning the neuromuscular junction with venom. For self-defense, electric eels leap from the water to directly electrify threats, efficiently activating nociceptors to deter their target. The latter behavior supports a legendary account by Alexander von Humboldt who described a battle between electric eels and horses in 1800. Finally, electric eels use high-voltage not only as a weapon, but also to efficiently track fast-moving prey with active electroreception. In conclusion, remarkable behaviors go hand in hand with remarkable physiology.

Predation and Crypsis in the Evolution of Electric Signaling in Weakly Electric Fishes

Eavesdropping by electroreceptive predators poses a conflict for weakly electric fish, which depend on their Electric Organ Discharge (EOD) signals both for navigation and communication in the dark. The EODs that allow weakly electric fish to electrolocate and communicate in the dark may attract electroreceptive predators such as catfishes and Electric Eels. These predators share with their prey the synapomorphy of passive electric sense supported by ampullary electroreceptors that are highly sensitive to low-frequency electric fields. Any low-frequency spectral components of the EOD make weakly electric fish conspicuous and vulnerable to attack from electroreceptive predators. Accordingly, most weakly electric fish shift spectral energy upwards or cloak low-frequency energy with compensatory masking signals. Subadults and females in particular emit virtually no low-frequency energy in their EODs, whereas courting males include a significant low-frequency component, which likely attracts females, but makes the signals conspicuous to predators. Males of species that coexist with the most predators tend to produce the least low-frequency signal energy, expressing sexual dimorphism in their signals in less risky ways. In these respects, electric signals follow the classic responses to opposing forces of natural and sexual selection, as exemplified in the visual signals of guppies and the acoustic signals of Tungara frogs. Unique to electric fish is that the electric signal modifications that help elude detection by electroreceptive predators are additions to the basal signal rather than losses of attractive components. These enhancements that enable crypsis are energetically costly, but have also provided the evolutionary substrates for subsequent sexual selection and species identity characters.

Dominance in Habitat Preference and Diurnal Explorative Behavior of the Weakly Electric Fish Apteronotus leptorhynchus.

Electrocommunication and -localization behaviors of weakly electric fish have been studied extensively in the lab, mostly by means of short-term observations on constrained fish. Far less is known about their behaviors in more natural-like settings, where fish are less constrained in space and time. We tracked individual fish in a population of fourteen brown ghost knifefish (Apteronotus leptorhynchus) housed in a large 2 m3 indoor tank based on their electric organ discharges (EOD). The tank contained four different natural-like microhabitats (gravel, plants, isolated stones, stacked stones). In particular during the day individual fish showed preferences for specific habitats which provided appropriate shelter. Male fish with higher EOD frequencies spent more time in their preferred habitat during the day, moved more often between habitats during the night, and less often during the day in comparison to low-frequency males. Our data thus revealed a link between dominance indicated by higher EOD frequency, territoriality, and a more explorative personality in male A. leptorhynchus. In females, movement activity during both day and night correlated positively with EOD frequency. In the night, fish of either sex moved to another habitat after about 6 s on average. During the day, the average transition time was also very short at about 20 s. However, these activity phases were interrupted by phases of inactivity that lasted on average about 20 min during the day, but only 3 min in the night. The individual preference for daytime retreat sites did not reflect the frequent explorative movements at night.

QSAR Study of Some 1,3-Oxazolylphosphonium Derivatives as New Potent Anti-Candida Agents and Their Toxicity Evaluation.

The incidence of invasive fungal infections caused by Candida spp. has increased continuously in recent decades, especially in populations of immunocompromised patients or individuals hospitalized with serious underlying diseases. Therefore, the goal of our study was the search for new potent Candida albicans inhibitors via the development of QSAR models that could speed up this search process. A number of the most promising 1,3-oxazol-4-yltriphenylphosphonium derivatives with predicted activities were synthesized and experimentally tested. Furthermore, the toxicity of the studied compounds was determined in vitro using acetylcholinesterase enzyme as a biological marker. The classification QSAR models were created using Random Forests (WEKA-RF), k-Nearest Neighbors and Associative Neural Networks methods and different combinations of descriptors on the Online Chemical Modeling Environment (OCHEM) platform. Аntifungal properties of the investigated compounds were performed using standard disk diffusion method. The enzyme inhibitory action of the compounds was determined by modified Ellman's method using acetylcholinesterase from the electric organ of Electrophorus electricus. Three classification QSAR models were developed by the WEKA-RF, k-NN and ASNN methods using the ALogPS, E-State indices and Dragon v.7 descriptors. The predictive ability of the models was tested through cross-validation, giving a balanced accuracy BA = 80-91%. All compounds demonstrated good antifungal properties against Candida spp. and slight inhibition of the acetylcholinesterase activity. The high percentage of coincidence between the QSAR predictions and the experimental results confirmed the high predictive power of the developed QSAR models that can be applied as tools for finding new potential inhibitors against Candida spp. Furthermore, 1,3-oxazol-4- yl(triphenyl)phosphonium salts could be considered as promising candidates for the treatment of candidiasis and the disinfection of medical equipment.

Calbindin-D28k expression in spinal electromotoneurons of the weakly electric fish Apteronotus leptorhynchus during adult development and regeneration.

Additive neurogenesis, the net increase in neuronal numbers by addition of new nerve cells to existing tissue, forms the basis for indeterminate spinal cord growth in brown ghost knifefish (Apteronotus leptorhynchus). Among the cells generated through the activity of adult neural stem cells are electromotoneurons, whose axons constitute the electric organ of this weakly electric fish. Electromotoneuron development is organized along a caudo-rostral gradient, with the youngest and smallest of these cells located near the caudal end of the spinal cord. Electromotoneurons start expressing calbindin-D28k when their somata have reached diameters of approximately 10μm, and they continue expression after they have grown to a final size of about 50μm. Calbindin-D28k expression is significantly increased in young neurons generated in response to injury. Immunohistochemical staining against caspase-3 revealed that electromotoneurons in both intact and regenerating spinal cord are significantly less likely to undergo apoptosis than the average spinal cord cell. We hypothesize that expression of calbindin-D28k protects electromotoneurons from cell death; and that the evolutionary development of such a neuroprotective mechanism has been driven by the indispensability of electromotoneurons in the fish's electric behavior, and by the high size-dependent costs associated with their production or removal upon cell death.

Sexual Size Dimorphism in the Macana Tigrina, Gymnotus javari (Gymnotidae, Gymnotiformes)

Gymnotiform electric fishes exhibit many forms of sexual dimorphism, including sexual size dimorphism (SSD), aspects of head, jaw, and electric organ morphology, and electric signal waveform. However, sexual dimorphism is poorly documented in banded electric fishes (Gymnotus), where most species are monomorphic in external appearance and electric signal waveforms. Gathering comparative data on species of Gymnotus has historically been hampered by their predatory and territorial lifestyles, in that they are usually dispersed in their preferred habitats and often collected individually. Here data are presented from a population of G. javari from the Peruvian Amazon, examined for SSD (n = 55) and electric organ discharge (EOD) amplitude (n = 162). These results are compared with a syntopic population of G. carapo (n = 61), and also with G. omarorum (n = 24) from a population in Uruguay. Unlike most congeners, G. javari exhibits pronounced sexual dimorphism in total length and body weight, and EOD pulse amplitude is correlated with total length. A review of several ecological hypotheses on body-size distributions of each sex as a balance of multiple competing selection pressures finds none of these hypotheses adequately explain SSD in G. javari. Examining SSD in biogeographic and phylogenetic contexts leads to the conclusion that selection across different environments (e.g., local habitats, geographic regions) and through evolutionary time may have contributed to the evolution of SSD in some but not all lineages of Gymnotus.

Neural activity in a hippocampus-like region of the teleost pallium is associated with active sensing and navigation.

Most vertebrates use active sensing strategies for perception, cognition and control of motor activity. These strategies include directed body/sensor movements or increases in discrete sensory sampling events. The weakly electric fish, Gymnotus sp., uses its active electric sense during navigation in the dark. Electric organ discharge rate undergoes transient increases during navigation to increase electrosensory sampling. Gymnotus also use stereotyped backward swimming as an important form of active sensing that brings objects toward the electroreceptor dense fovea-like head region. We wirelessly recorded neural activity from the pallium of freely swimming Gymnotus. Spiking activity was sparse and occurred only during swimming. Notably, most units tended to fire during backward swims and their activity was on average coupled to increases in sensory sampling. Our results provide the first characterization of neural activity in a hippocampal (CA3)-like region of a teleost fish brain and connects it to active sensing of spatial environmental features.