Effluent Total Inorganic Nitrogen Research Articles

A novel sulfur autotrophic denitrification in-situ coupled sequencing batch reactor system to treat low carbon to nitrogen ratio municipal wastewater: Performance, niche equilibrium and pollutant removal mechanisms

A novel integrated sulfur fixed-film activated sludge in SBR system (IS0FAS-SBR) was proposed to treat the low C/N ratio municipal wastewater. The effluent total inorganic nitrogen (TIN) and PO43−-P decreased from 17 mg/L and 3.5 mg/L to 8.5 mg/L and 0.5 mg/L, and higher nitrogen removal efficiency was contributed by the autotrophic denitrification. Microbial response characteristics showed that catalase (CAT), reduced nicotinamide adenine dinucleotide (NADH) and extracellular polymeric substance (EPS) alleviated the oxidative stress of sulfur carrier to maintain cell activity, while metabolic activity analysis indicated that the electron transfer rate was enhanced to improve mixotrophic denitrification efficiency. Meanwhile, the increased key enzyme activities further facilitated nitrogen removal and sulfur oxidation process. Additionally, the microbial community, functional proteins and genes revealed a niche equilibrium of C, N, S metabolic bacteria. Sulfur autotrophic in-situ coupled SBR system enlarged a promising strategy for treatment of low C/N ratio municipal wastewater.

Advanced nitrogen removal from low carbon nitrogen ratio domestic sewage via continuous plug-flow anaerobic/oxic/anoxic system: Enhanced by endogenous denitrification

An anaerobic/oxic/anoxic continuous plug-flow biorereactor was established to derive stable advanced nitrogen removal of oligotrophic domestic wastewater by setting a sludge dual-reflux system and a mixed liquid cross-flow system, while extending the hydraulic retention time in anoxic section. The effluent total inorganic nitrogen was 7.9 ± 2.2 mg N/L, with removal efficiency of 84 ± 3.9%. Results of nitrogen balance calculations indicated that the contribution of simultaneous nitrification and denitrification to total inorganic nitrogen loss in oxic region was 15% during stable stage, and the total inorganic nitrogen removal by endogenous-denitrification and enhanced exogenous-denitrification in the anoxic region was 39.9%. Prolongation of hydraulic retention time in anoxic segment is the critical reason for enhancing endogenous-denitrification, and cross-flow system is an important measure to improve exogenous-denitrification. This study provides new insights into bridging the gap between energy-saving and high-level nitrogen removal from municipal wastewater with low carbon to nitrogen ratios.

Effects of superficial gas velocity on the performance of an air-lift internal circulation partial nitrification-anammox granular sludge reactor

The airlift internal circulation reactor for partial nitrification-anammox (PNA-ALR) has the advantages of a small footprint, high mass transfer efficiency, and the ease of formation of granular sludge, thus making it an effective biological treatment for ammonia-containing wastewater. Although superficial gas velocity (SGV) is an essential parameter for PNA-ALR, it is unclear how the magnitude of SGV impacts nitrogen removal performance. In this study, the nitrogen removal efficiencies of five PNA-ALRs with different SGV were measured during feeding with synthetic municipal wastewater. At an optimal SGV of 2.35 cm s−1, the PNA-ALR consistently maintained the total inorganic nitrogen (TIN) removal efficiency at 76.31% and the effluent TIN concentration was less than 10 mg L−1. By increasing or decreasing the SGV, the nitrogen removal efficiency decreased to a range between 30% and 50%. At lower SGV, the dead space in the PNA-ALR was increased by 21.15%, and the feast/famine ratio of sludge increased to greater than 0.5, which caused a disruption in the structure, and a large loss of, granular sludge. Computational fluid dynamics (CFD) simulations showed operation at a higher SGV, resulting in excessive shear stress of 3.25 N m−2 being generated from bubble rupture in the degassing section. Fluorescent staining determined a decrease of 26.5% in viable bacteria. These results have improved our understanding of the effects of SGV on a PNA-ALR during mainstream wastewater treatment.

Nitrogen removal from high-saline municipal wastewater via anammox-based process driven by both nitritation and denitratation

Nitrogen removal from saline municipal wastewater has recently attracted attention; thus, developing energy-efficient technology such as anaerobic ammonium oxidation (anammox) for advanced nitrogen removal of hypersaline wastewater is significant. In this study, the long-term salt adaptation and evolution mechanisms of an anammox-based process driven by both nitritation (NH4+→NO2—, PN) and denitratation (NO3—→NO2—, PD) were investigated under salinities ranging from 1.0% to 3.0% (10–30 g NaCl/L). At 1.0% salinity, PN evolved into the major nitrite production pathway with thorough suppression of nitrite oxidation. At 1.5% salinity, nitrite reduction was inhibited along with suspended sludge loss due to poor sludge settleability. Under 2.0% salinity with only-biofilm, the highest nitrogen removal efficiency (81.2%) was observed with influent and effluent total inorganic nitrogen concentrations of 44.5 and 8.2 mg N/L, respectively. Notably, results showed that anammox bacteria occupied up to 1.07% of the microbial community (qPCR: 1.14×1011 copies/(g dry sludge)) and has strong adaptability to salinity. With salinity increases, anammox contribution kept over 85% and Ca. Kuenenia (0.57%→0.22%) was more tolerant than Ca. Brocadia (0.46%→0.02%). Metagenomic sequencing revealed that the multiple nitrite substrates production by Nitrosomonas (amoA-enriched), Denitratisoma and Denitromonas (narG-enriched) guaranteed the high anammox contribution. This study provides a multifaceted understanding of the anammox-based process, which can enable improved application in real saline wastewater treatment.

Improved nitrogen removal performance by enhanced denitratation/anammox as decreasing temperature for municipal wastewater treatment

Low temperature is one of the obstacles in mainstream anammox. This study aimed to evaluate the stability of a step-feed bioreactor dominated by denitratation/anammox (PD/A) for treating municipal wastewater with decrease in temperature (21.5 °C→12.6 °C). During long-term operation, nitrogen removal efficiency (NRE) was improved from 72.2% (21.5 °C→14.9 °C) to 77.2% (stable at 13.9 °C) with influent and effluent total inorganic nitrogen of 44.6 and 10.1 mg N/L. Better low temperature tolerance of nitrate reduction than nitrite reduction enhanced denitratation and increased nitrite substrates for anammox. Microbial structure analysis revealed that Ca. Brocadia increased by 167% and reached 0.27% on biofilm at low temperature. Finally, anammox contribution improved from 41.8% to 60.2%, saving organic carbon for denitrification, which was responsible for better NRE. Moreover, Ferribacterium, Dechloromonas, and Thauera were the major denitrifiers distributed in suspended sludge. Overall, this study provides an optimistic prospect for promoting anammox in mainstream applications at low temperature.

Ultra-high nitrogen removal from real municipal wastewater using selective enhancement of glycogen accumulating organisms (GAOs) in a partial nitrification-anammox (PNA) system

Integrating endogenous denitrification (ED) into partial nitrification-anammox (PNA) systems by adequately utilizing organics in municipal wastewater is a promising approach to improve nitrogen removal efficiency (NRE). In this study, a novel strategy to inhibit phosphorus-accumulating organisms (PAOs) by inducing phosphorus release and exclusion was adopted intermittently, optimizing organics allocation between PAOs and glycogen-accumulating organisms (GAOs). Enhanced ED-synergized anammox was established to treat real municipal wastewater, achieving an NRE of 97.5±2.2% and effluent total inorganic nitrogen (TIN) of less than 2.0 mg/L. With low poly-phosphorus (poly-P) levels (poly-P/VSS below 0.01 (w/w)), glycogen accumulating metabolism (GAM) acquired organics exceeded that of phosphorus accumulating metabolism (PAM) and dominated endogenous metabolism. Ca. Competibacter (GAO) dominated the community following phosphorus-rich supernatant exclusion, with abundance increasing from 3.4% to 5.7%, accompanied by enhanced ED capacity (0.2 to 1.4 mg N/g VSS /h). The enriched subgroups (GB4, GB5) of Ca. Competibcater established a consistent nitrate cycle with anammox bacteria (AnAOB) through endogenous partial denitrification (EPD) at a ∆NO2−-N/∆NH4+-N of 0.91±0.11, guaranteeing the maintenance of AnAOB abundance and performance. These results provide new insights into the flexibility of PNA for the energy-efficient treatment of low-strength ammonium wastewater.

Low energy-consumed process of integrated anammox and in-situ fermentation-based denitrification for ultra-efficient nitrogen removal from mainstream domestic sewage

Anammox is a promising biotechnology for nitrogen removal and gaining popularity in mainstream wastewater treatment. The efficient and low energy-consumed elimination of the intrinsic byproduct nitrate in anammox-mediated process is still a tough barrier and urgently demands resolving. To explore a newly green alternative for advanced nitrogen removal from domestic sewage, this study proposed a novel Integrated Anammox, Fermentation and Denitrification (IAFD) process. After 215-day operation, total inorganic nitrogen (TIN) removal efficiency markedly increased from 72.6% to 94.1% and effluent TIN dropped from 7.9 mg L−1 to 1.8 mg L−1 with influent TIN of 56 mg L−1. Through in-situ activated sludge fermentation, 37.341 mg COD·L−1·d−1 organics was yielded, causing a COD/NO3−-N of 8.9, Soluble protein (63.8%), polysaccharide (10.6%) and acetic acid (13.9%) were primary components. Candidatus_Brocadia (0.8%) and Candidatus_Competibacter (4.6%) were dominant functional bacteria, which contributed 81.1% and 18.9% to nitrogen elimination, respectively. Robust anammox (3.37 mg N·g VSS−1·h−1), fermentation-assisted denitrification and denitritation synergistically boosted N removal. The novel process required no external carbon, reduced 59.5% aeration and 35.8% external sludge. The novel process showed significantly environmental and economical advantages of low operation expenses and energy cost with no external carbon requirement, 59.5% aeration and 35.8% external sludge reduction. This work provided an energy-neutral and sustainable scheme for the advanced nitrogen removal of municipal wastewater and sludge disposal in WWTPs.

Start-up of the combined anaerobic ammonium oxidation and solid phase denitrification process and microbial characterization analysis

To improve the nitrogen removal performance of anaerobic ammonia oxidation (anammox)-based processes, a modified anaerobic baffled reactor (ABR) equipped with a packed bed of poly-3-hydroxybutyrate-cohyroxyvelate (PHBV) was employed to start up the combined anammox and solid phase denitrification (SPD) process for the treatment of nitrogen-rich wastewater. Results showed that a stable removal of total inorganic nitrogen (TIN) of 98.5 % was achieved with a nitrogen loading rate of 0.46 kg·(m3·d)−1, decreasing the effluent TIN concentration to <2 mg·L−1. Throughout the operation period of 180 days, sludge granulation in three compartments of the ABR was promoted by a stepwise decrease in the hydraulic retention time. The anammox reaction and endogenous denitrification mainly occurred in the first two compartments with inorganic feeding and contributed >86 % of TIN removal. 16S rRNA gene amplicon sequencing showed that anammox communities, dominated by genera Candidatus Kuenenia and Candidatus Brocadiaceae, were enriched in granular sludges with symbiotic heterotrophic bacteria. Co-cultures of hydrolytic bacteria (Burkholderiales, Kapabacteriales, and Clostridium-sensu-stricto-7) and denitrifying bacteria (Comamonadaceae, Rhodocyclaceae, and Denitratisoma) were detected within the biofilm on the surface of PHBV particles. PHBV hydrolysate input effectively drove heterotrophic denitrification in the successive compartment and increased the complexity of microbial co-occurrence networks. These findings provide insight into the synergistic mechanisms between anammox and SPD and present a feasible combined process application for advanced biological nitrogen removal.

A novel process for simultaneous biological nutrient removal and waste activated sludge reduction in one-stage system without aeration

This study presented a novel process for simultaneous biological nutrient removal and waste activated sludge reduction in one-stage municipal wastewater treating system without aeration on the basis of endogenous partial denitrification, anammox and Candidatus Phosphoribacter dominated biological phosphate removal with sludge reduction (EPDA/PSR). The effluent total inorganic nitrogen and phosphate concentrations were 0.1 mg/L and lower than detection limit (0.1 mg/L), respectively, without dosing external carbon sources. Compared with partial denitrification-anammox process, the daily sludge production in EPDA/PSR process reduced by 50.8 %. The percentage of intact cells measured by flow cytometry reduced from 92.4 % to 77.3 % with the increase of aromatic protein, DNA content of supernatant, soluble microbial byproduct-like substance and decline of particle size. The sludge stabilization structure was destructed, and intracellular organics were released, which resulted in the reduction of sludge production. Microbial community structure at transcriptional level indicated that really working Candidatus Phosphoribacter (phosphorus accumulating organisms with fermentation capacity), Candidatus Competibacter (endogenous partial denitrification bacteria) and Candidatus Brocadia (anammox bacteria) reached 68.4 %, 9.84 % and 8.02 %, respectively, and Candidatus Kuenenia (anammox bacteria) did not play a role in EPDA/PSR system. The novel one-stage EPDA/PSR process realized high efficient biological nutrient removal, achieved waste activated sludge reduction and saved 100 % of aeration energy consumption cost and external carbon source dosage cost, which effectively developed internal carbon source and provided a new idea for wastewater treatment.

Mainstream anammox driven by micro-oxygen nitrification and partial denitrification using step-feed for advanced nitrogen removal from municipal wastewater

This study developed a novel integrated nitrification, partial denitrification (PD), and anammox (INPDA) process in micro-aerobic sequencing batch reactor using step-feed strategy treating municipal wastewater. The results showed that the INPDA could achieve a 94.5 ± 1.1% total inorganic nitrogen (TIN) removal efficiency with effluent TIN concentration as low as 2.3 mg/L. The micro-aerobic with low dissolved oxygen concentration of 0.36 ± 0.01 mg/L allowed complete ammonium oxidation to nitrate. The applied step-feed by optimizing influent distribution ratio to 5:3 provided sufficient organics for nitrate reduction to nitrite. According to mass balance analysis, anammox was the dominant nitrogen removal pathway (87.9%). Microbial analysis indicated that aerobic nitrifiers Nitrosomonas (1.2%) and Nitrospira (0.9%) ensured full nitrification, while PD bacteria Thauera (27.1%) conducted labor metabolism coordination with anammox bacteria Candidatus Brocadia (1.3%) for nitrogen removal. These findings demonstrate that the INPDA has a good prospect for energy-efficient municipal wastewater treatment.

Advanced nitrogen removal from municipal sewage via partial nitrification-anammox process under two typical operation modes and seasonal ambient temperatures

A novel two-stage partial nitrification-anammox (PN-A) process was developed, achieving nitrogen removal from low carbon/nitrogen ratio municipal sewage under two typical operational modes and seasonal ambient temperatures. When complete nitritation-anammox was performed at temperatures greater than 19.4 °C, the effluent concentration of total inorganic nitrogen (TIN) was 4.1 mg/L, corresponding to a nitrogen removal efficiency (NRE) of 94.3 %. In contrast, when partial nitritation-anammox was performed at temperatures below 19.4 °C, the effluent TIN was 12.3 mg/L, corresponding to a NRE of 83.6 %. The relative abundance of Nitrosomonas and Nitrosomonadaceae increased from 0.02 % to 0.28 %, while Ca. Brocadia decreased from 1.85 % to 1.30 %, with the contribution of anammox to nitrogen removal being highest under low temperatures (19.4℃ to 13.8℃), at 59.0 %. This novel two-stage PN-A process provides a new approach for the stable operation of wastewater treatment plants (WWTPs) under low ambient temperatures.

Double anammox process in the AOAO process of treating real low C/N sewage: Validation, enhancement, and quantification of the contribution of anammox in the oxic zone

Enhancement of anaerobic ammonium oxidation (anammox) process and enrichment of anammox bacteria in the oxic zone of mainstream sewage treatments are complex. Also, quantification of the anammox contribution for nitrogen removal in the oxic zone is hindered owing to the simultaneous occurrence of anammox and nitrification. An alternating anaerobic/oxic/anoxic/oxic bioreactor whose oxic zone boosted partial nitrification coupling anammox (PN/A) and anoxic zone boosted partial denitrification coupling anammox (PD/A), respectively, was operated to treat real sewage for >380 days. Desirable nitrogen removal (effluent total inorganic nitrogen (TIN) of 4.7 ± 1.9 mg N/L) was obtained from low carbon/nitrogen (3.6 ± 0.5) sewage with ammonium concentration of 52.5 ± 2.2 mg N/L in the influent. Under the condition of dissolved oxygen (DO) of 1.5–3 mg/L, anammox bacteria was still enriched within the aerobic biofilms, with the relative abundance increasing to 8.2 % (day 345) from 0 % (no biomass on day 1), which was higher than the value in the flocculent sludge (0.03 %) (P < 0.001). PN driven by flocculent sludge with high activity of ammonium oxidized bacteria (AOB) ensured sufficient nitrite supply for the anammox process with the existence of continuous DO. During the steady operation period, the maximum anammox contribution in the oxic zone was quantified to be 10.6 % by withdrawing aerobic biofilms from the system.

Stable nitrogen removal in the novel continuous flow anammox system under deteriorated partial nitrification: Significance and superiority of the anaerobic-oxic-anoxic–oxic operation mode

The collapse of mainstream anammox system caused by deterioration of partial nitrification (PN) is easy to occur and it is vital to quickly restore the stable nitrogen elimination performance. Herein, a novel continuous push-flow anaerobic-oxic-anoxic–oxic (AOAO) process treating sewage was used to restore the nitrogen elimination performance rapidly under deteriorated PN. The increased abundances of Nitrospira and Candidatus Nitrotoga was responsible for the deterioration of PN. Effluent total inorganic nitrogen of 8.7 mg N/L and a stable nitrogen removal rate of 0.083 kg N/m3·d were obtained with the aerobic hydraulic retention time (HRT) of 3.75 h even PN deteriorated. Endogenous partial denitrification coupled anammox in the anoxic zone was essential to maintain stable nitrogen removal under the deterioration of PN and the anammox contribution increased from 17.2 % to 23.6 %. The AOAO system shows robustness on nitrogen removal even PN deteriorated under the decrease of HRT from 16 to 12 h.

Rapid cultivation and enrichment of anammox bacteria solely using traditional activated sludge as inoculum and biocarrier in low-strength real sewage treatment

In low-ammonia sewage anammox process, cultivation and enrichment of anammox bacteria (AnAOB) is a challenge especially from traditional activated sludge. To this end, a novel strategy solely using activated sludge as inoculum and biocarrier in a dynamic fixed-bed reactor was proposed in this study. During 115-day operation, excellent performance was achieved with influent total inorganic nitrogen (TIN) and effluent TIN of 55.3 mg·L−1 and 4.1 mg·L−1, respectively. Rapid enrichment of AnAOB (doubling time: 8.5 days) was demonstrated by augmented specific anammox activity (trace value to 1.85 mg N·g VSS−1·h−1) and increased hzsB gene number (106 to 109 copies·g−1 dry sludge), with predominance of Candidatus_Brocadia. Large-flocs aggregate was the primary habitat for AnAOB with highest abundance and capacity. The distinctive sludge properties, symbiotic microbial interactions and dynamic operation scheme facilitated AnAOB growth and retention. This study provides a simple, economic and workable approach for the start-up of mainstream anammox process.

Enhanced nitrogen removal from low COD/TIN mainstream wastewater in a continuous plug-flow reactor via partial nitrification, simultaneous anammox and endogenous denitrification (PN-SAED) process

A continuous plug-flow reactor with anaerobic/front-aerobic/anoxic/post-aerobic zones, where partial nitrification occurred in the front-aerobic zone, followed by simultaneous anammox and endogenous denitrification in the anoxic zone (PN-SAED), was built up to treat municipal wastewater. Alternating anoxic/aerobic conditions and longer anoxic duration facilitated stable partial nitrification. The nitrite accumulation ratio (NAR) was maintained at 97.4 ± 1.2%, with temperatures between 13.3℃ to 19.8℃. Candidatus Brocadia were naturally enriched in-situ from the anoxic zone with relative abundances of 31.93% and 6.67% on the agitator blade and carriers, respectively. High removal efficiencies of total inorganic nitrogen (TIN) (95.1 ± 1.9%) and effluent TIN (2.6 ± 1.1 mg N/L) were acquired from low COD/TIN (3.4 ± 0.4) municipal wastewater with anammox contribution of 13.5%±5.8% to TIN removal. The PN-SAED process is a promising mainstream nitrogen removal method.

Mainstream double-anammox driven by nitritation and denitratation using a one-stage step-feed bioreactor with real municipal wastewater

A novel double-anammox process for advanced mainstream nitrogen removal was established using step-feed sequencing batch reactor (SBR) system with integration of suspend sludge and biofilms. Following optimization of influent distribution ratio, the effluent total inorganic nitrogen (TIN) was < 10.2 mg N/L, with influent TIN of 43.4 mg N/L, and anammox contributed 71.4% to TIN removal. Biological processes and batch tests revealed that gradient C/N reduction promoted denitratation/anammox in anoxic stage, and simultaneous nitritation and anammox were achieved in oxic stage. Specially, anammox maintained on biofilms with abundance over 109 copies/ (g dry sludge). High-throughput sequencing revealed that Thauera and Nitrosomonas were enriched in flocs. Furthermore, metagenomic sequencing confirmed that Thauera owns narG and napA (NO3–→NO2–) and Nitrosomonas owns amoA (NH4+→NO2–), support stable NO2– supply for double-anammox. This mainstream anammox-dominant process could potentially be used for stable nitrogen removal in municipal wastewater treatment plants.

Mainstream partial denitrification-anammox (PD/A) for municipal sewage treatment from moderate to low temperature: Reactor performance and bacterial structure

Anammox is sensitive to temperature, which can limit its practical application in wastewater treatment. In this study, a step-feed anoxic-oxic (A/O) process coupled with PD/A was operated steadily from 26.8 °C to 13.1 °C for wastewater treatment for 200 days. The effluent total inorganic nitrogen (TIN) and phosphorus concentrations were 10.2 mg/L and 0.29 mg/L at C/N ratio of 4.6 and 15.0 °C even with increasing nitrogen loading rate (NLR). The anammox activity was 5.60 mg NH4+-N/gMLSS/d even at 14 °C, moreover, anammox abundance on the biocarriers increased with decreasing temperature. It was observed that the effect of partial denitrification (PD) was enhanced under low temperature, thus the contribution of anammox for nitrogen removal was improved. The pathway of anammox for nitrogen removal accounted for 48% and the effect of effluent did not deteriorate under low temperature. This study states that PD/A has advantages under low temperature operation, which is suitable for treatment of wastewater with low C/N ratio.

Synergistic partial denitrification, anammox and in-situ fermentation (SPDAF) process for treating domestic and nitrate wastewater: Response of nitrogen removal performance to decreasing temperature

A synergistic partial denitrification, anaerobic ammonium oxidation (Anammox), and in-situ fermentation (SPDAF) system was established to solve problems of wastewater treatment plants (WWTPs) in combined treatment of domestic sewage, and nitrate wastewater discharged from industrial areas. The SPDAF system was started up at decreasing temperatures (26.8–18.9 ℃), and remained robust at abrupt temperature drop and drastic temperature fluctuations (20.7–14.1 ℃). The influent and effluent total inorganic nitrogen (TIN) were 97.0 ± 3.7 mg/L and 10.3 ± 4.0 mg/L, respectively. In-situ fermentation supplemented electron donors for NO3–-N reduction. A high TIN removal efficiency, of 89.5 ± 3.9% was obtained. Specifically, Anammox contributed 90.9 ± 5.2% to TIN removal. Furthermore, the abundances of hydrolysis and acidogenesis bacteria were 14.02% and 29.47% in the low and high zones, respectively, which promoted fermentation and the use of complex organics. This study provided novel insights for actual operation of WWTPs.

Improving performance and efficiency of partial anammox by coupling partial nitrification and partial denitrification (PN/A-PD/A) to treat municipal sewage in a step-feed reactor

Improving contribution and nitrogen removal efficiency (NRE) of partial anammox in municipal wastewater is a researching hotspot. This study developed an innovative PN/A-PD/A process with fixed biocarriers in anaerobic/anoxic chambers for actual sewage treatment in a typical step-feed reactor over 390 days. Two coupled pathways providing continuous NO2– (partial nitrification in oxic chambers and partial denitrification in anaerobic/anoxic chambers) for anammox were introduced to the process, achieving 47% nitrogen loss by anammox in stable phase. The influent and effluent total inorganic nitrogen (TIN) were 51.3 and 11.0 mg/L, respectively, even with chemical oxygen demand (COD)/TIN ratio of 2.9. Anammox activity improved from 6.52 to 9.68 mg NH4+-N/gMLSS/d and abundance on the biocarriers raised to 3.16 × 1010 gene copies/g dry sludge. Overall, this study confirmed partial anammox, spatially coupled with partial nitrification and partial denitrification via oxic/anoxic distribution with step feed mode, as an alternative for application of mainstream anammox.

Achieving advanced nitrogen removal in a novel partial denitrification/anammox-nitrifying (PDA-N) biofilter process treating low C/N ratio municipal wastewater

For achieving mainstream anammox, a novel partial denitrification/anammox-nitrifying (PDA-N) biofilter process to treat municipal wastewater was developed. This process achieved a total inorganic nitrogen (TIN) removal efficiency of 81%, with an average effluent TIN of 7.31 mg·L-1, when the ratio of influent chemical oxygen demand (COD) to TIN was 3.2. Approximately 97% of the TIN was removed by anammox in the PDA biofilter. Nitrite was provided by partial denitrification for anammox. Partial denitrification was driven by Thaurea in the middle and lower regions of the PDA biofilter, while anammox was mainly driven by Candidatus Brocadia in the middle and upper regions. When treating real municipal wastewater, the TIN was efficiently removed in the PDA-N biofilter, with the effluent TIN of 5.96 mg·L-1. Anammox played a primary role, achieving approximately 98% of the TIN removal. Compared to the traditional nitrification/denitrification process, this process can economize organic carbon demand and oxygen consumption.