Testicular atrophy is a condition impairing the reproductive health of male chicken. Lithiasis or stone formation in testicles or epididymis adds to the gravity of the problem. The present study describes a case of unilateral testicular atrophy with epididymal lithiasis in a one-year-old male Gramasree cock. The carcass, was brought to the Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Pookode, for postmortem examination. The lesions were recorded and tissues for histopathology were collected in 10% neutral buffered formalin, sectioned, and stained using routine hematoxylin and eosin staining procedure. Grossly, there was diffused congestion of the testicular tunic with atrophy of right testis. The epididymis was congested with multifocal white spots. Histologically, seminiferous tubules were severely degenerated. The efferent ductules of epididymis were dilated with basophilic amorphous and granular liths in multiple locations. Sperm stasis was evident in a few ductules and rete testes. The lith formation might have caused sperm stasis and degeneration and atrophy of the testis.