Microbiologically Induced Calcite Precipitation (MICP) technology offers a promising method for stiffness reinforcement of offshore wind turbines (OWTs). However, edge scour around microbial reinforcement raises concerns about potential stiffness degradation. This study examines the effects of edge scour on the lateral responses of rigid piles reinforced with precast microbial reinforcement using a low-pH one-phase grouting method. Results from static tests, validated by numerical simulations, demonstrated that MICP technology bonded loose sand grains with the pile, forming a bio-reinforced pile with a larger diameter in the shallow soil layer, which significantly enhanced the original pile's bearing capacity and stiffness. However, edge scour reduced the embedment depth of the bio-reinforced pile, leading to a decrease in its bearing capacity and stiffness. Geometrically, protection width was found to have a relatively greater influence on stiffness and capacity compared to protection thickness. Additionally, symmetric cyclic loading tests were conducted to evaluate the effects of edge scour on backbone curves, secant stiffness, and damping ratio. Although MICP-based reinforcement notably enhanced both the secant stiffness and damping ratio of the piles, its effectiveness was completely lost once the scour depth reached the reinforcement thickness of the bio-reinforced soil block.