Effect Of Pretreatment Research Articles

Preventive Effect of Pretreatment with Intravenous Metoclopramide on Incidence of Post Laparoscopic Surgery Nausea and Vomiting

Introduction: Common complications following anesthesia include Postoperative nausea and vomiting (PONV), which leads to patient dissatisfaction and discomfort. The incidence of PONV after inhalational general anesthesia is up to 30% [1]. When anesthetics are used with no prophylaxis, and in certain high-risk patients, the incidence of PONV can reach up to 70% [2]. Furthermore, laparoscopic surgeries are associated with an even higher incidence of PONV (40%-75%) [3]. This makes PONV one of the most prevalent post-operational complaints [4].Methods: This case control study includes 79 patients with high PONV risk scores scheduled for laparoscopic cholecystectomy under general anesthesia in Duhok hospitals. 40 patients received 8 mg (2ml) metoclopramide intravenously as pretreatment, while 39 patients received normal saline (2ml) as the control group. After 48 hours, we called patients and asked them about the incidence and severity of PONV.Result: statically insignificant differences in the incidence of PONV between both groups (p-value 0.91). The incidence of PONV in this study was (27.8%) which correlates with the PONV risk score mean (2.48) and was statistically significant with a p-value of less than 0.01 and a 95% confidence score (2.13, 18.32).Conclusion: PONV risk score is effective in the prediction of the incidence of PONV, and Metoclopramide is ineffective as a single drug in the prevention of PONV in high-risk patients.

Effects of coagulation pre-treatment on chemical and microbial properties of water-soil-plant systems of constructed wetlands

Chemical coagulation has gained recognition as an effective technique to enhance the removal efficiency of pollutants in wastewater prior to their entry into a constructed wetland (CW) system. However, its potential impact on the chemical and microbial properties of soil and plant systems within CWs requires further research. This study investigated the impact of using ferric chloride (FeCl3) as a pre-treatment stage for dairy wastewater (DWW) on the chemical and microbial properties of water-soil-plant systems of replicated pilot-scale CWs, comparing them to CWs treating untreated DWW. CWs treating amended DWW had better performance than CWs treating raw DWW for all water quality parameters (COD, TSS, TP, and TN), ensuring compliance with the EU wastewater discharge directives. Soil properties remained mostly unaffected except for pH, calcium and phosphorus (P), which were lower in CWs treating amended DWW. As a result of lower nitrogen (N) and P loads, the plants in CWs receiving FeCl3-amended DWW had lower N and P contents than the plants of raw DWW CWs. However, the lower loads of P into amended DWW CWs did not limit the growth of Phragmites australis, which were able to accumulate trace elements higher than CWs receiving raw DWW. Alpha and Beta-diversity analysis revealed minor differences in community richness and composition between both treatments, with only 3.7% (34 genera) showed significant disparities. Overall, the application of chemical coagulation produced superior effluent quality without affecting the properties of soil and plant of CWs or altering the functioning of the microbial community.

Effect of Pretreatments on the Chemical, Bioactive and Physicochemical Properties of Cinnamomum camphora Seed Kernel Extracts.

Cinnamomum camphora seed kernels (CCSKs) are rich in phytochemicals, especially plant extracts. Phytochemicals play a vital role in therapy due to their strong antioxidant and anti-inflammatory activities. Extracts from CCSK can be obtained through multiple steps, including pretreatment, extraction and purification, and the purpose of pretreatment is to separate the oil from other substances in CCSKs. However, C. camphora seed kernel extracts (CKEs) were usually considered as by-products and discarded, and their potential bioactive values were underestimated. Additionally, little has been known about the effect of pretreatment on CKE. This study aimed to investigate the effects of pretreatment methods (including the solvent extraction method, cold pressing method, aqueous extraction method and sub-critical fluid extraction method) on the extraction yields, phytochemical profiles, volatile compounds and antioxidant capacities of different CKE samples. The results showed that the CKE samples were rich in phenolic compounds (15.28-20.29%) and alkaloids (24.44-27.41%). The extraction yield, bioactive substances content and in vitro antioxidant capacity of CKE pretreated by the sub-critical fluid extraction method (CKE-SCFE) were better than CKEs obtained by other methods. CKE pretreated by the solvent extraction method (CKE-SE) showed the best lipid emulsion protective capacity. Moreover, the volatile substances composition of the CKE samples was greatly influenced by the pretreatment method. The results provided a fundamental basis for evaluating the quality and nutritional value of CKE and increasing the economic value of by-products derived from CCSK.

Effect of hydrothermal pretreatment on leachate fed Scenedesmus sp. biomass solubilization and biogas production

The aim of the present study was to assess the effect of hydrothermal pretreatment on the solubilization and anaerobic digestion (AD) of Scenedesmus sp. biomass. At first, the microalgae was cultivated in 5% fresh leachate (FL) to recover nutrients such as nitrogen and phosphorus. Scenedesmus sp. grown in 5% FL obtained 100%, 77% and 97% removal efficiency of ammonium nitrogen (NH4+ - N), total Kjeldahl nitrogen (TKN) and phosphate phosphorous (PO43− -P), respectively. In the following step, the hydrothermal pretreatment of Scenedesmus sp. biomass was carried out at 120, 150 and 170 °C and retention time of 0, 30 and 60 min to evaluate its solubilization and biogas production through AD in batch test. Soluble chemical oxygen demand (sCOD) increased by 260% compared to untreated microalgae at 170 °C for 60 min. In comparison to untreated microalgae, the highest increase in biogas (70%) and methane yield (100%) was observed for 150 °C and 60 min pretreated microalgae as a consequence of hydrothermal pretreatment. Hydrothermal pretreatment has shown effectiveness in enhancing biomass solubilization and increasing biogas yield. Nevertheless, further research at the pilot scale is necessary to thoroughly evaluate the potential and feasibility of hydrothermal pretreatment for full-scale implementation.

Effect of low-temperature thermal-alkali pre-treatment on waste activated sludge solubilisation

The study was aimed to examine the effects of alkaline and thermal-alkaline pre-treatment on sewage sludge solubilisation with different alkali and its doses ranging from 40 mg alkali/g TS to 120 mg alkali/g TS. Initially, the effect of alkali pre-treatment was monitored with four alkali at room temperature, to further enhance soluble chemical oxygen demand (sCOD) and reduce treatment time, thermal-alkaline pre-treatment was performed with varying temperature from 40 to 70 °C. To explore and optimise the effects of temperature, alkali, and alkali doses on COD solubilisation, response surface methodology was employed. Statistical analysis of the variance was conducted to identify the relationship between experimental data and the proposed model. The thermal-alkaline process was optimised for maximum COD solubilisation with optimal conditions achieved at 65.61 °C of 66.57 mg alkali /g TS for NaOH and 27 °C of 20 mg/g TS for KOH. The maximum COD solubilisation achieved at these optimised conditions for NaOH and KOH was 21.28 % and 16.13 %, respectively. The results demonstrated a significant improvement in protein and carbohydrate concentration and a 16–20 % reduction in sludge solid content after thermal-alkali pre-treatment.

Effects of the Hot Water Pre-treatments and Storage Durations on the Seed Germination of Falcata (Falcataria Falcata (L.) Greuter & R. Rankin)

Seed dormancy in Falcataria falcata (L.) Greuter & R. Rankin is well documented. In order to attain a higher seed germination rate, the effect of hot water pre-treatment and storage duration was determined in this study. This study found a highly significant difference (p < 0.05) between the mean Cumulative Germination Percentage (CGP) between treatments. Hot water pre-treatment significantly increased the CGP of seeds. However, storage duration and its interaction with different hot water pre-treatment did not have a statistically significant effect on the CGP of seeds. Overnight (12 hrs) soaking of seeds in water with an initial temperature of 80oC yields the highest CGP regardless of the storage duration. On development, it was found that radicle emergence of imbibed seeds generally starts within 1-2 days. Bangladesh J. Bot. 53(2): 249-255, 2024 (June)

Effect of Pre-treatments on Extraction Yield and Quality of Fruit Pectin: A Review

Effect of Pre-treatments on Extraction Yield and Quality of Fruit Pectin: A Review

Effect of Pre-treatments and Open Sun Drying of Grapes on its Quality

Effect of Pre-treatments and Open Sun Drying of Grapes on its Quality

Inhibitory effect of all-trans retinoic acid on ferroptosis in BeWo cells mediated by the upregulation of heme Oxygenase-1

IntroductionThis study aimed to explore the association between ferroptosis, a newly identified type of cell death, and the role of retinoic acid in developing pregnancy complications. Therefore, the effects of all-trans retinoic acid (ATRA) on ferroptosis susceptibility in BeWo cells were assessed to understand abnormal placental development. MethodsBeWo cells were used as surrogates for cytotrophoblasts. The effect of ATRA on ferroptosis sensitivity was assessed on BeWo cells pretreated with ATRA or dimethyl sulfoxide (DMSO; control), following which the LDH-releasing assay was performed. The effects of ATRA pretreatment on the antioxidant defense system (including glutathione [GSH], mitochondrial membrane potential, and heme oxygenase-1 [HMOX1]) in BeWo cells were assessed using assay kits, RT-qPCR, and HMOX1 immunostaining. To evaluate the effect of ATRA on BeWo cells, HMOX1 was silenced in BeWo cells using shRNA. ResultsATRA pretreatment increased ferroptosis resistance in BeWo cells. Although with pretreatment, qPCR indicated upregulation of HMOX1, no significant change was observed in the GSH levels or mitochondrial membrane potential. This was corroborated by intensified immunostaining for heme oxygenase-1 protein (HO-1). Notably, the protective effect of ATRA against ferroptosis was negated when HO-1 was inhibited. Although HMOX1-silenced BeWo cells exhibited heightened ferroptosis sensitivity compared with controls, ATRA pretreatment counteracted ferroptosis in these cells. DiscussionATRA pretreatment promotes BeWo cell viability by suppressing ferroptosis and upregulating HMOX1 and this can be used as a potential therapeutic strategy for addressing placental complications associated with ferroptosis.

Fucoidan as a Promising Drug for Pain Treatment: Systematic Review and Meta-Analysis.

Fucoidan is a polymer of L-fucose and L-fucose-4-sulphate naturally found in marine sources that inhibits p-selectin, preventing neutrophil recruitment to the site of injury. Fucoidan is employed in many studies as a tool to investigate the contribution of neutrophils to pain, showing analgesic effects. We performed a systematic review and meta-analysis to quantify the analgesic effects of pretreatment with fucoidan reported in the available preclinical studies. In addition, we summarized the articles which have studied the therapeutic effects of fucoidan in pathological pain at preclinical and clinical levels. The results of this systematic review reveal that pretreatment with fucoidan is a powerful tool which reduces neutrophil infiltration by 70-90% at early time points. This meta-analysis showed that preventative treatment with fucoidan produced a significant pain reduction. In addition, several preclinical studies have observed that fucoidan treatment reduces the pain that is associated with various pathologies. Finally, fucoidan has also been tested in several clinical trials, with some degree of analgesic efficacy, but they were mostly small pilot studies. Considering all the above information, it can be concluded that fucoidan is not only a preclinical tool for studying the role of neutrophils in pain but also a promising therapeutic strategy for pain treatment.

Effects of Freeze-Thaw Pretreatment Combined with Hot Air on Snake Gourd (Trichosanthes anguina L.).

Snake gourd is a seasonal vegetable with a high water content and medicinal value, but the short harvest period limits the large-scale application of snake gourd. Therefore, the effects of freeze-thaw pretreatment (FT) combined with hot air (HD) on the drying characteristics, active ingredients and bioactivities of snake gourd were investigated. The results showed that FT pretreatment reduced browning and shortened the drying time by 44%; the Page model was the best fit for describing the drying process. The polysaccharide contents (21.70% in alcoholic extract (TG1) and 44.34% in water extract (TG2)) and total phenol contents (1.81% in TG1 and 0.88% in TG2) of snake gourd pretreated by FT-HD were higher than those of snake gourd pretreated by the corresponding HD treatment. The FT pretreatment decreased the molecular weight of snake gourd polysaccharides and increased the molar ratio of glucose. The extracts pretreated by FT-HD showed greater chemical, cellular antioxidant capacity and α-amylase and α-glucosidase inhibition than those pretreated by HD. FT-HD can be recommended for achieving a short drying time and high quality of snake gourd and can be used for the drying of other fruits and vegetables.

Effects of cold plasma pretreatment before different drying process on the structural and functional properties of starch in Chinese yam

This article compared the effects of hot air drying (HAD), infrared drying (IRD), and cold plasma (CP) as a pretreatment on the structure, quality, and digestive characteristics of starch extracted from yam. As the most commonly used drying method, HAD was used as a control. SEM and CLSM images showed that all treatments preserve the integrity of the yam starch. CP caused some cracks and breaks in the starch granules. IRD did not destroy the crystal structure of starch molecules, but made the spiral structure tighter and increased short-range orderliness. However, CP led to the depolymerization and dispersion of starch molecular chains, resulting in a decrease in average molecular weight and relative crystallinity. These molecular conformation changes caused by different processes led to differences in solubility, swelling power, pasting parameters, digestion characteristics, and functional characteristics. This study provided an important basis for the reasonable drying preparation and utilization of yam starch.

CDK4/6 inhibition enhances T-cell immunotherapy on hepatocellular carcinoma cells by rejuvenating immunogenicity

Hepatocellular carcinoma (HCC) poses a significant clinical challenge, necessitating the integration of immunotherapeutic approaches. Palbociclib, a selective CDK4/6 inhibitor, has demonstrated promising efficacy in preclinical HCC models and is being evaluated as a novel therapeutic option in clinical trials. Additionally, CDK4/6 inhibition induces cellular senescence, potentially influencing the tumor microenvironment and immunogenicity of cancer cells. In this study, we conducted comprehensive bioinformatic analyses using diverse HCC transcriptome datasets, including bulk and single-cell RNA-sequencing data from public databases. We also utilized human and mouse HCC cells to investigate functional aspects. Primary T cells isolated from mouse blood were employed to assess T cell immunity against HCC cells. Results revealed that CD8+ T-cell infiltration correlates with improved outcomes in HCC patients with suppressed CDK4/6 expression. Moreover, CDK4/6 expression was associated with alterations in the immune landscape and immune checkpoint expression within the liver tumor microenvironment. Furthermore, we found that treatment with Palbociclib and Doxorubicin induces cellular senescence and a senescence-associated secretory phenotype in HCC cells. Notably, pretreatment with Palbociclib augmented T cell-mediated cytotoxicity against HCC cells, despite upregulation of PD-L1, surpassing the effects of Doxorubicin pretreatment. In conclusion, our study elucidates a novel mechanism by which CDK4/6 inhibition enhances T-cell-associated cancer elimination and proposes a potential therapeutic strategy to enhance T-cell immunotherapy on HCC.Graphical abstract

Effect of Multi-Mode Divergent Ultrasound Pretreatment on Hardness, Microstructure and Digestion of Acid-Induced Whey Protein Gels.

Whey protein was pretreated with multi-frequency ultrasound in mono-, dual-, and tri-frequency modes. The effect of multi-frequency ultrasound pretreatment on the hardness, chemical forces, and microstructure of acid-induced whey protein gel was investigated. Whey protein gels pretreated with dual- and tri-frequency ultrasound showed higher hardness (p < 0.05) and a denser network than mono-frequency ultrasound and control. Moreover, they had higher hydrophobic interaction and lower disulfide bonds than the control (p < 0.05). The effect of gel properties on digestion was evaluated using an in vitro static model. Whey protein gels pretreated with dual- and tri-frequency ultrasound had a higher proportion of large fragments in the oral boluses than in the control. Large fragments (>3.35 mm) in those samples were resistant to gastric digestion. Moreover, the tri-frequency ultrasound pretreatment of whey protein gel released the least free amino group during gastric digestion. In contrast, whey protein gel with the mono-frequency ultrasound pretreatment released the highest amount of free amino acid group during intestinal digestion. Findings from this study suggests that gel hardness and network density could modulate the digestion behaviors of protein gels.

Anaerobic Two-Phase Co-Digestion for Renewable Energy Production: Estimating the Effect of Substrate Pretreatment, Hydraulic Retention Time and Participating Microbial Consortia

Green and sustainable economies have recently become a key issue in long-term growth and well-being. Co-digestion of various waste materials in an eco-friendly way through biogas production has become the preferred method for their utilization and valorization. The possibility of hydrogen and methane yield maximization depends on the most suitable alkali reagent for pretreatment of waste lignocellulosic material, which was revealed in batch tests to determine the hydrogen production potential. The mixture for digestion consisted of pretreated wheat straw mixed with waste algal biomass in a ratio of 80:20 (w/w). The maximum hydrogen yield was achieved after applying sodium hydroxide thermoalkaline pretreatment, with a two-fold higher yield than the untreated control. Hydrogen production was stable and methane was not present in the resultant gas. The influence of the hydraulic retention time (HRT) on the maintenance of cascade installation was studied. The maximum daily concentration of hydrogen was achieved at an HRT of 2 days—42.5% H2—and the maximum concentration of methane was 56.1% at an HRT of 6 days. Accumulation of volatile fatty acids was registered in the first step and their depletion was noted in the second one. The obtained values of the cellulose content demonstrated that it was utilized by up to 2.75% in the methanogenic bioreactor at the end of the process. Metagenomics analyses revealed the bacteria Thermocaproicibacter melissae (44.9%) and Clostridium cellulosi (41.9%) participated in the consortium, accomplishing substrate hydrolysis and acidogenesis in the first stage. Less in abundance were Thermoanaerobacterium butyriciformans, Calorimonas adulescens, Pseudomonas aeruginosa and Anaerocolumna chitinilytica. Methanogenesis was performed by an archaeon closely related to Bathyarchaeota (99.5%) and Methanobacterium formicicum. The most abundant bacterial strains in the methanogenic fermenter were Abyssalbus ytuae (30%), Proteiniphilum acetatigenes (26%) and Ruficoccus amylovorans (13%).

Sustainable mortar reinforced with recycled glass fiber derived from pyrolysis of wind turbine blade waste

Pyrolysis technology has recently made significant progress in recycling millions of tons of wind turbine waste (WTB). Short fibers represent its main product (∼70 wt%), which needs further studies to explore its future applications. In this context, this work aims to the valorization of the recycled glass fiber (rGF) derived from pyrolysis of WTB (at 550 °C) in the production of rGF-reinforced mortar composite (rGF/M) for sustainable building applications. The research began by subjecting the derived rGF to sieving (rGFs), washing (rGFw), and oxidation (rGFo) pretreatments for purification and removing any impurities, organic residues, and char particles. Afterward, the chemical degradation of the treated rGF in a strong alkaline cement solution with pH = 14 was studied for up to 90 days. In parallel, the mortar composite samples were fabricated at 1 wt% of the treated rGF batches (rGFr, rGFw, and rGFo) to study the effect of different pretreatments on their microstructure, dispersion, compressive strength, flexural strength, and water absorption capabilities behavior after different curing periods (28 and 90 days). The results showed that rGFo (7.84%) showed a lower degradation rate versus rGFw (16.41%) and rGFr (30.76%). Meanwhile, the highest curing period (90 days) contributed to improving the properties of the mortar composites in general, especially in the case of using rGFo which led to improved compressive strength (15%) and reduced absorption coefficient (38%) and cumulative water content (32%) with a uniform distribution of short fibers in the matrices and a slight increase in flexural strength compared to control mortar sample (8.6 MPa). While rGFs provides better flexural strength (9.3 MPa) with a 12% improvement. Based on these results it can be concluded that rGFo has high potential in sustainable building materials with high competition with virgin glass fiber.

In-situ grown ZnO-nanorods of slippery liquid-injected porous surface coating on Mg alloy with hydrophobic, durable, and anti-corrosion properties

In this work, an in-situ self-grown nano-ZnO/epoxy-based slippery lubricant liquid-injected porous surface (SLIPS) coating with a unique bilayer structure is reported to achieve robustness, hydrophobicity, and anti-corrosion properties. Dense epoxy coated ZnO nanoparticles are used as the anti-corrosion barrier layer. Self-grown ZnO nanorods provide a robust micro- and nano-rough porous structure. The lubricant perfluoropolyether (PFPE) is strongly locked into the porous structure via low surface energy material pretreatment and capillary effect. This SLIPS coating has a high water contact angle (WCA) value and has excellent repellency to various liquids. The SLIPS surface maintains a high contact angle of more than 115° even after 20 days of immersion in corrosive solutions and withstand the erosion of corrosive solutions with pH = 3. It also has a very low corrosion current density of 8.97 × 10−12 A/cm2 and the Rct (the charge transfer resistance) of optimum SLIPS remained 1.41 × 108 ohm cm2 after 70 days immersion in 3.5 wt% NaCl solution, which is in the same order of magnitude as the initial value. The SLIPS coating with excellent mechanical stability, super-hydrophobicity, anti-fouling, chemical stability, and long-term corrosion resistance performance possesses tremendous application potential in long-term corrosion protection of Mg alloys.

Dexmedetomidine mitigates lipopolysaccharide-induced acute lung injury by modulating heat shock protein A12B to inhibit the toll-like receptor 4/nuclear factor-kappa B signaling pathway

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS): Life-threatening medical conditions characterized by high morbidity and mortality rates, where the inflammatory process plays a crucial role in lung tissue damage, especially in models induced by lipopolysaccharide (LPS). Heat shock protein A12B (HSPA12B) has strong anti-infammatory properties However, it is unknown whether increased HSPA12B is protective against LPS-induced ALI. And Dexmedetomidine (DEX) is a potent α2-adrenergic receptor (α2-AR) agonist that has been shown to protect against sepsis-induced lung injury, however, the underlying mechanisms of this protection are not fully understood. This study utilized bioinformatics analysis and an LPS-induced ALI model to explore how DEX alleviates lung injury by modulating HSPA12B and inhibiting the Toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) signaling pathway. Results indicate that HSPA12B overexpression and DEX pre-treatment markedly mitigated LPS-induced lung injury, which was evaluated by the deterioration of histopathology, histologic scores, the W/D weight ratio, and total protein expression, tumor necrosis factor-alpha (TNF-α), and interleukin-1β (IL-1β) in the BALF, and the levels of NO, MDA,SOD and MPO in the lung. Moreover, HSPA12B overexpression and DEX pre-treatment significantly reduces lung injury and inflammation levels by upregulating HSPA12B and inhibiting the activation of the TLR4/NF-κB signaling pathway. On the contrary, when the expression of HSPA12B is inhibited, the protective effect of DEX pre-treatment on lung tissue is significantly weakened.In summary, our research demonstrated that the increased expression of AAV-mediated HSPA12B in the lungs of mice inhibits acute inflammation and suppresses the activation of TLR4/NF-κB pathway in a murine model of LPS-induced ALI. DEX could enhance HSPA12B and inhibit the initiation and development of inflammation through down-regulating TLR4/NF-κB pathway.These findings highlight the potential of DEX as a therapeutic agent for treating ALI and ARDS, offering new strategies for clinical intervention.

Enhancing dark fermentative hydrogen production from wheat straw through synergistic effects of active electric fields and enzymatic hydrolysis pretreatment

Hydrogen, a clean and sustainable energy source, faces challenges from energy-intensive pre-processing technologies. This study explores the synergistic enhancement of active electric fields on enzymolysis of wheat straw and hydrogen production through dark fermentation. The active electric field enzymolysis system improved the adsorption capacity of wheat straw to cellulase, increasing cellulase activity by 18.0 %, causing a 39.1 % increase in reducing sugar content. In the active fermentation system, Clostridium_sensu_stricto_1 activity was enhanced in the first stage, increasing hydrogenase activity by 23.0 %, prolonging the first hydrogen production peak. Elevated reducing sugars were observed in the second stage, with Prevotella_9 and Bacteroides becoming the dominant hydrogen-producing bacteria in the third stage, leading to a second hydrogen production peak. Overall, cumulative hydrogen production was enhanced by 50.9 % compared to the control. The synergistic pretreatment with an active electric field and cellulase provides a novel approach for efficiently utilizing wheat straw.

Effects of Red and Blue Light on the Growth, Photosynthesis, and Subsequent Growth under Fluctuating Light of Cucumber Seedlings.

The effects of red and blue light on growth and steady-state photosynthesis have been widely studied, but there are few studies focusing on dynamic photosynthesis and the effects of LED pre-treatment on cucumber seedlings' growth, so in this study, cucumber (Cucumis sativus L. cv. Jinyou 365) was chosen as the test material. White light (W), monochromatic red light (R), monochromatic blue light (B), and mixed red and blue lights with different red-to-blue ratios (9:1, 7:3, 5:5, 3:7, and 1:9) were set to explore the effects of red and blue light on cucumber seedlings' growth, steady-state photosynthesis, dynamic photosynthesis, and subsequent growth under fluctuating light. The results showed that compared with R and B, mixed red and blue light was more suitable for cucumber seedlings' growth, and the increased blue light ratios would decrease the biomass of cucumber seedlings under mixed red and blue light; cucumber seedlings under 90% red and 10% blue mixed light (9R1B) grew better than other treatments. For steady-state photosynthesis, blue light decreased the actual net photosynthetic rate but increased the maximum photosynthetic capacity by promoting stomatal development and opening; 9R1B exhibited higher actual net photosynthetic rate, but the maximum photosynthetic capacity was low. For dynamic photosynthesis, the induction rate of photosynthetic rate and stomatal conductance were also accelerated by blue light. For subsequent growth under fluctuating light, higher maximum photosynthetic capacity and photoinduction rate could not promote the growth of cucumber seedlings under subsequent fluctuating light, while seedlings pre-treated with 9R1B and B grew better under subsequent fluctuating light due to the high plant height and leaf area. Overall, cucumber seedlings treated with 9R1B exhibited the highest biomass and it grew better under subsequent fluctuating light due to the higher actual net photosynthetic rate, plant height, and leaf area.