Effect Of Plant Growth Regulators Research Articles

Investigating the effect of plant growth regulators in the endodormancy release in apricot by a metabolomic approach

Endodormancy is one of the most studied physiological processes in perennial plants like apricot. This period is vital both for the tree survival against the adverse climatic conditions of winter and for obtaining a proper flowering and fruit set. Many studies have remarked the importance of chill accumulation as the limiting factor for endodormancy release. The increase of mean temperatures caused by climate change has been seriously endangering this process during the last decades. Because of this, plant growth regulators for promoting endodormancy release have spread worldwide. However, due to the toxicity and the irregular efficiency, there is a great necessity of developing new environment-friendly regulators for promoting endodormancy release. In this 3-year study, we applied four different commercial plant growth regulators to the Flopría apricot cultivar. Two of them, Broston® and Erger® were the most effective ones to advance endodormancy release. The physiology and biochemistry behind these treatments were studied by a non-target metabolomic and expression analysis in flower buds. Metabolic groups, like phospholipids, only varied in treated samples, whereas others like by-products of L-Phe metabolism, or ABA significantly varied in both types of samples throughout endodormancy release. Finally, to validate these results, solutions of phospholipids, phenylpropanoids, or ABA, among others, were applied for the first time to apricot trees, showing, i.e., that phospholipids treated-trees released from endodormancy two weeks earlier than control. This study aims to be an initial stage for the elaboration of environmentally safe regulators in apricot, with a potential in other Prunus and temperate fruit tree species.

Effects of Different Plant Growth Regulators on Seed Germination, Seedling Growth and Establishment of Papaya (Carica papaya) Cv. Pusa Nanha

Papaya (Carica papaya L.) is an economically significant tropical fruit, but its cultivation is often hindered by low seed germination rates and poor seedling vigor. The present study aims to investigate the effects of different Plant Growth Regulator on seed germination, seedling growth and establishment of papaya (Carica papaya) Cv. Pusa Nanha. at The Department of Horticulture, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, during the period 2023-24. The experiment was laid in completely randomized block design with three replications and ten treatment combinations. viz, T0 (Control), T1 (NAA 100ppm), T2 (NAA 150ppm), T3 (NAA 200ppm), T4 (GA3 100ppm), T5 (GA3 150ppm),T6 (GA3 200 ppm), T7 (IAA 100ppm), T8 (IAA 150ppm), T9 (IAA 200ppm). Seeds were soaked in respective PGR solutions for 12 hours before sowing and various parameters related to Germination Parameters, Growth Parameters, Survival and Establishment were evaluated. On the basis of present experimental findings, it is concluded that treatmentT6(GA3 200ppm) performed best in term of Germination percentage (100%), Seedling vigour index (823.33), Number of leaves (10.00), Plant height (16.83 cm), Chlorophyll content (41.30 SPAD value) and stem girth (0.56 cm) Whereas T2 (NAA 150ppm) performed best in term of Leaf length (8.83 cm), Leaf width (8.50 cm), Leaf area (59.53 cm2) and Establishment Percentage (82.20%). The lowest observation was recorded in T0 (Control). These findings suggest that specific PGR treatments can substantially enhance the germination and growth parameters of papaya seedlings, potentially leading to improved crop yields.

Effect of Preharvest Treatment Using Jasmonic Acid and Methyl Jasmonate on the Physicochemical Properties and Antioxidant Activities of Red-fleshed Dragon Fruit (Hylocereus polyrhizus L.)

This study investigated the effect of exogenous plant growth regulators (PGR), namely jasmonic acid (JA) and methyl jasmonate (MeJA), on the physicochemical properties of flesh and peels of red-fleshed dragon fruit (Hylocereus polyrhizus). The fruit was sprayed with 100 and 1,000 ppm of JA and MeJA at 15 and 22 days of anthesis and harvested after 35 days. Then, the flesh and peels were analyzed for total soluble solids (TSS), total betacyanins, betanin, total phenolics (TP), total flavonoids (TF), and color characteristics. The fruit peels contained significantly higher (p<0.05) TP and antioxidant activities compared to flesh. No significant difference was detected between the variables in the peels, except for significantly higher (p<0.05) of total betacyanins (~295.6 and ~299.9 mg/100 g) and TP (~614.1 and 566.1 mg GAE/100 g) were recorded in control and 100 ppm MeJA, respectively. In the flesh, 1,000 ppm MeJA-treated fruit possessed the highest total betacyanins (~139.2 mg/100 g), betanin (~356.0 mg/g), TP (~244.9 mg GAE/100 g), TF (~329.0 mg CE/100 g), Trolox equivalent antioxidant capacity (TEAC) (63.2 µmol TE/g) and reducing power (~21.5 µmol TE/g). Overall, 1,000 ppm MeJA was more effective in enhancing the accumulation of bioactive compounds and antioxidant activities in the flesh of red-fleshed dragon fruit compared to other PGR treatments.

The use of thioxopyrimidine derivatives for the regulation of vegetative growth of wheat

The work is devoted to the screening of new effective wheat plant growth regulators based on synthetic compounds, thioxopyrimidine derivatives. The wheat (Triticum aestivum L.) variety Oksamit Mironivskyi was used as the object of the study. Morphometric parameters (average length of shoots and roots (mm), and average biomass of 10 plants (g)) of 4-week-old wheat plants were measured. Treatment of wheat plants with thioxopyrimidine derivatives at a concentration of 10-6 M contributed to the growth and development of shoots and roots compared to control wheat plants treated with distilled water. The regulatory effect of thioxopyrimidine derivatives on the vegetative growth of wheat plants was similar to the regulatory effect of auxin IAA (1H-indol-3-yl)acetic acid) or synthetic compounds, derivatives of sodium and potassium salts of 6-methyl-2-mercapto-4-hydroxypyrimidine (Methyur, Kamethur) and N-oxide-2,6-dimethylpyridine (Ivin) used in the same concentration of 10-6 M. The correlation between the growth regulatory effect and the chemical structure of synthetic compounds, thioxopyrimidine derivatives was established. The selected most active synthetic compounds, thioxopyrimidine derivatives, are proposed to be used to improve the vegetative growth of wheat (T. aestivum L.) variety Oksamit Mironivskyi.

Effect of Plant Growth Regulators on Growth, Fruit Yield and Quality of Kharif Season Bitter Gourd (Momordica charantia L.)

The present was conducted at Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during the session 2022 - 2024. The experiment was laid out in randomized block design with three replications, and the study consists of ten treatment combinations including control. The best treatment was T12 (NAA200ppm) &T11 (NAA150ppm) which shows highest values in all the parameters viz., day to germination (10.20), survival % (71.02%), vine length at last harvest (228.02), number of days to first male flower appearance (43.10), number days to first female flower appearance (53.67), number of days to 50% flower first picking (61.85), number fruit per vine (31.23), average fruit length (20.79cm), average fruit diameter (4.34), average fruit weight (88.05 g), average fruit yield per plant (49.83 kg), TSS (189 °brix), vitamin C (85.33). Increased flowering, fruit yield, quality and economics might be due to the increased absorption of nutrients when given as foliar application.

Effect of Plant Growth Regulators on Growth, Yield and Quality of Tomato (Lycopersicon esculentum L.)

The present investigation was carried out at the Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj, Uttar Pradesh during Kharif 2021-22 with a view to identify the effects of different doses of plant growth regulators on growth, yield and quality of Tomato variety TMTH-288. The experiment was laid in Randomized block design with 13 treatments and 3 replications with different combination in nano fertilizers. Under this experiment, overall, 13 combinations was taken including control. PGRs comprised of GA3, 2,4-D, Triacontanol and NAA were given. According to the current research, the current study found that the use of plant growth regulators (PGRs) had a significant positive impact on the growth and yield of tomato. Among the treatments tested, T3 (GA3 40 ppm) showed the most favorable results in terms of plant height, early flowering and maturing. It also demonstrated superior performance in terms of fruit weight, polar and radial length of fruit, number of fruits per plant, and yield per plant.

Enhancing the phytoextraction efficiency of heavy metals in acidic and alkaline soils by Sedum alfredii Hance: A study on the synergistic effect of plant growth regulator and plant growth-promoting bacteria

Plant growth regulators (PGR) and plant growth-promoting bacteria (PGPB) have the potential in phytoremediation of heavy metals (HMs) contaminated soils. However, their sole application may not yield the optimal results, thus necessitating the combined application. The present study aimed to enhance the phytoremediation efficiency of Sedum alfredii Hance (S. alfredii) in acidic and alkaline soils through the combination of PGR (Brassinolide, BR) and PGPB (Pseudomonas fluorescens, P. fluorescens). The combination of BR and P. fluorescens (BRB treatment) effectively increased the removal efficiency of S. alfredii for Cd, Pb, and Zn by 355.2 and 155.3 %, 470.1 and 128.9 %, and 408.4 and 209.6 %, in acidic and alkaline soils, respectively. Moreover, BRB treatment led to a substantial increase in photosynthetic pigments contents and antioxidant enzymes activities, resulting in a remarkable increase in biomass (86.71 and 47.22 %) and dry mass (101.49 and 42.29 %) of plants grown in acidic and alkaline soils, respectively. Similarly, BRB treatment significantly elevated the Cd (109.4 and 71.36 %), Pb (174.9 and 48.03 %), and Zn levels (142.8 and 104.3 %) in S. alfredii shoots, along with cumulative accumulation of Cd (122.7 and 79.47 %), Pb (183.8 and 60.49 %), and Zn (150.7 and 117.9 %), respectively. In addition, the BRB treatment lowered the soil pH and DTPA-HMs contents, while augmenting soil enzymatic activities, thereby contributing soil microecology and facilitating the HMs absorption and translocation by S. alfredii to over-ground tissues. Furthermore, the evaluation of microbial community structure in phyllosphere and rhizosphere after remediation revealed the shift in microbial abundance. The combined treatment altered the principal effects on S. alfredii HMs accumulation from bacterial diversity to the soil HMs availability. In summary, our findings demonstrated that synergistic application of BR and P. fluorescens represents a viable approach to strengthen the phytoextraction efficacy of S. alfredii in varying soils.

Effect of Plant Growth Regulators on Growth, Yield and Quality of Chilli (Capsicum annuum L.)

Chili peppers are fruit-bearing plants from the Capsicum genus, and they are known for their spicy and pungent flavors. Chili peppers thrive in warm climates and can be grown in various types of soil. They are typically cultivated as annual plants, although some varieties can be perennial in suitable conditions Therefore, the present investigation was carried out at the Central Research Farm, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj, Uttar Pradesh during Kharif 2021-22 with a view to identify the effects of different doses plant growth regulators and its role in growth, yield and quality of Chilli variety TMPH-443. The experiment was laid in Randomized block design with 13 treatments and 3 replications with different combination in plant growth regulators. Under this experiment, overall, 13 treatment was taken including control. Different plant growth regulators (PGRs) used comprised of NAA, GA3, 2-4 D and Triacontanol all four at different doses. According to the current research, the use of Plant growth regulators (PGRs) had a significantly positive impact on the growth and development of Chillies. Among the various treatments that were evaluated, T5 (NAA @ 40 ppm spray) yielded the most favourable results in terms of growth viz., plant height, leaf area, early flowering and maturation and yield viz., fruit weight, length of fruit, fruit girth, number of fruits per plant, and yield per plant.

Spore Germination of Diplazium simplicivenium Holtt. (Athyriaceae) in Peninsular Malaysia

Diplazium simplicivenium Holtt. a species of fern in Peninsular Malaysia. This study reports an efficient method for D. simplicivenium spore sterilisation and the effect of plant growth regulators (PGRs) via green globular bodies (GGB). Sterilisation with 0.1% mercuric chloride effectively allowed spore germination. The result showed that the culture media supplemented with 0.5 mg/L gibberellin positively affects the weight of GGB and the number of shoots with significant differences (ANOVA, p < 0.05). The soaking technique established in this study for spore sterilisation is an efficient approach, and the optimal plant and concentration of plant hormones were identified. This procedure can be applied to other indigenous ferns and closely similar species.

Effect of phosphorus and plant growth regulators on growth and yield of blackgram

Effect of phosphorus and plant growth regulators on growth and yield of blackgram

Effect of Plant Growth Regulators on Economics and Harvest Index of Garlic (Allium sativum L.) under Terai region of West Bengal

Effect of Plant Growth Regulators on Economics and Harvest Index of Garlic (Allium sativum L.) under Terai region of West Bengal

Impact of Micronutrients and Plant Growth Regulators on Brinjal (Solanum melongena L.) Growth, Yield and Quality

The present research work conducted to find out the effect of plant growth regulators and micronutrients on growth, yield, and quality of Brinjal (Solanum melongena L.) under open field condition. The experiment was designed in a Randomized Complete Block Design (RCBD) with three replicates. The experiment was conducted on Horticulture farm at Sher-e-Bangla Agricultural University; Dhaka, Bangladesh from July 2022 to June 2023. The experimental treatments included three levels of PGR i.e. No PGR, 200 ppm gibberellic acid (GA3) and 100 ppm Naphthaleneacetic acid (NAA) and three levels of micronutrients i.e.no micronutrients, 200 ppm Boron and 200 ppm Zn. The treatments had a substantial effect on growth, yield, and quality of Brinjal. GA3 treated plants performed better than NAA and no PGR treated plants and Zn produced higher yields and quality than Boron and no micronutrients treated plants in most of the parameters. Considering treatment combination of PGR and micronutrients, plant height, leaf area (471.33 cm2), SPAD value (52.95), number of flowers plant-1 (65.33), number of fruits plant-1 (58.38), yield plant-1 (3312.8g), lipid (0.35%) were observed highest from the treatment combination of 200 ppm GA3 with 200 ppm Zinc whereas dry weight of whole plant (158.80 g), individual fruit weight (67.33g) and fiber (12.28%) were observed from the treatment combination of 100 ppm NAA with 200 ppm zinc but in case of number of branch (14.00) and Ash (8.44%) were observed from the treatment of 100 ppm NAA application . Maximum parameter showed lowest value under no treatment combination. This study suggests that foliar application of GA3 with micronutrients such as Zinc during flowering stage of eggplant is the most effective strategy for improving the nutritional quality of Brinjal fruits and increasing crop yields, both of which will help to reduce micronutrient malnutrition and hunger worldwide.

Effectiveness of Nanoformulated Plant Growth Regulators in the Production and Propagation of High-value Crops and Ornamentals

Among the major classes of plant hormones, auxins, gibberellins, and cytokinins are widely used for plant propagation. These hormones influence the physiological and developmental processes of plants such as root initiation, apical dominance, seed germination, leaf expansion, and development of buds, flowers, and fruits. Its exogenous application was found to significantly improve the growth of several important plants. This study aimed to determine the effectiveness of plant growth regulators (PGRs) derived from naturally occurring plant growth-promoting bacteria (PGPBs) in the production and propagation of selected crops. In this study, indole- 3-acetic acid (IAA) and gibberellic acid (GA) were extracted from naturally occurring PGPB Bacillus sp. The extracted hormone was nanoformulated for controlled release and increased uptake in plants. The nanoformulated hormones were applied in the propagation of coffee and the production of eggplant and ornamentals. Results showed that the application of nanoformulated IAA (Nano-IAA) significantly increased the survival rate of coffee compared to the commercially available auxin. Nano-IAA increased the germination rate of eggplant in acidic soil and root emergence of Bougainvillea over the negative control (water) but was comparable to that of the commercially available auxin. The nanoformulated GAs and commercially available

Effect of Plant growth regulators and micro-nutrient on growth and herbage yield of forage sorghum (Sorghum bicolor L.)

Effect of Plant growth regulators and micro-nutrient on growth and herbage yield of forage sorghum (Sorghum bicolor L.)

High Efficiency In vitro Whole Plant Regeneration via Desiccated Callus in Oryza sativa cv. MTU1010

For most people across the planet, rice is also considered as most beneficial crops in terms of fulfilling every day's energy and nutritional needs. Worldwide food safety is seriously threatened since severe limitations on rice output caused by both abiotic and biotic variables. Even though rice features much more developed regeneration mechanism than other agricultural crops, majority of desi cultivars continue to stay as impervious to genetic alterations and regeneration. Therefore, among preliminary steps in the transgenic plant generation necessitates to improve tissue culture procedures to produce viable plants via embryogenic calli mediated regeneration. In this study, we reporting that enhanced rate of plant regeneration was observed from mature seed-derived embryogenic calli of the indica rice cultivar MTU1010 following partially desiccation procedure. We studied the effects of several plant growth regulators on the efficiency of inducing embryogenic callus and regeneration of whole plantlets. The MS-CIM medium with 2.0 mg-1 2,4, D showed the highest callus induction percentage. Maximum rate of shoot regeneration (92%) was observed from 48hr partially dehydrated calli, when enriched with high cytokinin (2 mg/l kinetin) and lower auxin (0.5 mg/l NAA) concentrations. Calli that had been moderately dehydrated showed significantly greater regeneration rates than undehydrated calli. Upon being transferred onto a rooting medium, the well-rooted plantlets were shifted to shaded glass house for hardening. Hence, this approach is rather simple to apply and can be employed in genetic modification studies alongside other biotechnological endeavours.

Effectiveness of Rhodococcus erythropolis strain OPI-01 on the fungal development in winter wheat

Abstract. Behzad A, Diakite S, Astarkhanova TS, Pakina EN, Allen D, Mohammadi PM, Diarra O, Saquee FS. 2024. Effectiveness of Rhodococcus erythropolis strain OPI-01 on the fungal development in winter wheat. Biodiversitas 25: 1063-1070. One of the means of sustainable wheat production is using bacterial pesticides, which can synthesize phytohormonal substances with antibacterial and antifungal action. Therefore, the work aimed to study the effect of different doses of a new growth regulator Restart, ZH (Rhodococcus erythropolis D strain OPI-01) on winter wheat yield. The findings of field experiments on the effect of plant growth regulator R. erythropolis strain OPI-01 method of pre-sowing treatment of seeds (at 0.1 L/t and 0.2 L/t), and soil spraying just before sowing (-0.5 l/ha) revealed that on average, during the 2020-2021 and 2021-2022 seasons, the number of productive stems increased by a margin of 23-30, the number of grains in 1 ear increased in the experimental units by 16.2-19.4%, and the length of the ear by 0.3-0.7cm, gluten varied from 32.3 to 33.8%, yield increased from 27.8-33.0% relative to the control. Disease severity and incidence of powdery mildew, brown rust and Septoria disease in plots amended withR. erythropolis strain OPI-01 was 2-fold less than in the control plots. Research trials show the efficacy of R. erythropolis strain OPI-01 under field conditions as a plant growth regulator and a potential microbial fungicide against some foliar diseases of winter wheat. It would be exciting to study the mechanism of R. erythropolis on wheat pathogens in vitro and in planta which are still less developed in the literature.

Impact of Diverse Plant Growth Regulators on Quality Traits of Garlic (Allium sativum L.)

The present study was conducted at the instructional cum research farm of the Department of Plantation Crops and Processing of Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar, India during rabi season for consecutive two years (October-2019 to March-2020 and October-2020 to march-2021) to investigate the “Effect of Different Plant Growth Regulators on Quality attributes of Garlic (Allium sativum L.)” with various growth regulators (GA3@ 50 ppm, GA3 @ 100 ppm, GA3 @ 150 ppm, NAA @ 50 ppm, NAA @ 100 ppm, NAA @ 200 ppm, Kinetin @ 10 ppm, Kinetin @ 20 ppm, Kinetin @ 40 ppm and Control (Distilled water). In view of, effect of different plant growth regulators on quality attributes has observed significant variation. Results pertaining to total soluble solids, significantly highest TSS recorded under GA3 @100 ppm treated plots (32.10 °Brix), however, dry matter content of bulb was highest at GA3 @ 50 ppm (33.63%). Interestingly, highest oleoresin content of the bulb was also reported from the GA3 @ 50 ppm treated plots (0.24 g) with lowest physiological loss of bulb weight (7.04%). The control plots treated with distilled water was produced qualitatively inferior bulbs. The aim of the study provided valuable results to determine the potential growth regulator for enhancing post-harvest quality attribute of garlic by employing methodology of Randomized Block Design comprising of three replications with ten treatments. Based on the current experimental results, it may be concluded that foliar application of GA3 @ 50 ppm proved the best over other plant growth regulators.

In vitro Callus Regeneration and Chlorophyll Content Estimation in Glycine max (L.) variety from Uttarakhand, India

Soybean (Glycine max) is considered one of the most substantial produced crops across the globe because of its high nutritional value. It is a great alternative for lactose-intolerant patients and vegans to fulfil their daily protein requirements. Soybean has various varieties depending upon the colour of their seed coat. In the past few years, the consumption of Soybean has increased which demands higher production, better yield, and better seed quality. Conventional propagation methods fail to fulfil such demands. The alternate method of plant tissue culture ensures rapid mass propagation, better yield, and quality of the plants. However, the technique is often beset with challenges of low-field performance of tissue culture-raised plants due to defective chloroplast machinery. The present study investigates the effect of various plant growth regulators (PGRs) on in vitro propagation of soybean cultivars from different regions of Uttarakhand, India, and their effect on chlorophyll content in the regenerated tissues.

ВОЗДЕЙСТВИЕ РЕГУЛЯТОРА РОСТА РАСТЕНИЙ КОРНЕВИНА НА УКОРЕНЯЕМОСТЬ СОРТОВ И ФОРМ ГРУШИ В УСЛОВИЯХ ИСКУССТВЕННОГО ТУМАНА

Pear is a seed crop that grows and is consumed all over the world, grows on trees and is harvested in the Northern Hemisphere from late summer to October. Plant growth regulators are such chemicals that are capable of various green and lignified cuttings to increase the root system, they are also able to increase yields. Kornevin is an effective plant growth regulator (phytohormone). It is a biological product that stimulates root formation. The drug has a mild effect, is not phytotoxic, eliminates overdoses. It has a long-lasting prolonged effect. Green pear cuttings K-1, K-2, OHF 333, Piro II had the greatest rootability when using the plant growth regulator kornevin (30.0 mg/l) (from 85.0 to 87.7%). Without the use of plant growth regulators, the forms K-1, K-2, OHF 333, Piro II had high rooting rates of green pear cuttings (from 70.5 to 75.9%). The highest growth height when using the plant growth regulator kornevin (30.0 mg/l) was possessed by the rootstock forms of the Caucasian pear, 4-26, 4-39, K-1, K-2, OHF 333, Piro II, this indicator varied from 12.5 to 14.0 cm. The largest diameter of the conditional root neck (from 1.3 to 1.6 cm) had the forms of pears Caucasian, 4-26, 4-39, K-1, K-2, OHF 333, Piro II and varieties ‘Pervomayskaya’, ‘Yakovlevskaya’, ‘Avgustovskaya Rosa’, ‘Irista’, ‘Chudesnitsa’, ‘Fevral'skiy Suvenir’. The largest number of roots (from 7.5 to 8.5 pcs.) were demonstrated by the forms of the Caucasian pear, 4-26, 4-39, K-1, K-2, OHF 333, Piro II. The largest root length (from 9.0 to 9.8 cm) was possessed by the rootstocks of the Caucasian pear, 4-26, 4-39, K-1, K-2, OHF 333, Piro II and varieties ‘Nika’, ‘Avgustovskaya Rosa’, ‘Irista’, ‘Fevral'skiy Suvenir’. Without the use of plant growth regulators, the largest plant height (from 10.0 to 12.0 cm) was possessed by the forms of Caucasian pear, 4-26, 4-39, K-1, K-2, OHF 333, Piro II. The largest number of roots (from 5.0 to 6.6 pcs.) had pear shapes 4-39, K-2, OHF 333, Piro II and varieties ‘Svetlyanka (k)’, ‘Nika’, ‘Pervomayskaya’. The largest root length without the use of plant growth regulators was characterized by the forms of the Caucasian pear, 4-26, 4-39, K-1, K-2, OHF 333, Piro II and varieties ‘Nika’, ‘Fevral'skiy Suvenir’, this indicator varied from 7.0 to 7.8 cm.

Effect of Different Plant Growth Regulators on In-vitro Micropropagation of Banana Cultivar Grand Naine (Musa spp.)

Banana (Musa sapientum) is one of the most important fruit crop cultivated in India. It is widely grown in more than 150 countries, producing on an average of 105 million tons of fruit per annum. Grand naine Banana variety is the most commonly cultivated banana with high yielding plantain. This variety mainly grown in the region of Uttar Pradesh state. Present study mainly focuses on the in-vitro micropropagation method which is described for banana cv. Grand naine. For this study, healthy sword suckers of three months old banana plantlets was used as explants. The explants was sterilized thoroughly and cultured on MS (Murashige and Skoog) media supplemented with the different concentration and combination of auxin and cytokinin like BAP, IAA and NAA which were used for the optimization of best plant growth regulator combination for multiple shoot generation and shoot growth. Among all the tested cytokinin 5 mg/l BAP along with 1mg/l IAA in medium concentration performed well and produced healthy shoots further at higher concentration of all tested cytokinin showed declining effect within the growth parameters. For the rooting purpose, MS media was supplemented with different levels of IBA, IAA and NAA. Results indicated that rooting was highly promoted in the medium supplemented with 0.5 mg/L IAA + 1.5 mg/L IBA.