High Efficiency In vitro Whole Plant Regeneration via Desiccated Callus in Oryza sativa cv. MTU1010

Abstract

For most people across the planet, rice is also considered as most beneficial crops in terms of fulfilling every day's energy and nutritional needs. Worldwide food safety is seriously threatened since severe limitations on rice output caused by both abiotic and biotic variables. Even though rice features much more developed regeneration mechanism than other agricultural crops, majority of desi cultivars continue to stay as impervious to genetic alterations and regeneration. Therefore, among preliminary steps in the transgenic plant generation necessitates to improve tissue culture procedures to produce viable plants via embryogenic calli mediated regeneration. In this study, we reporting that enhanced rate of plant regeneration was observed from mature seed-derived embryogenic calli of the indica rice cultivar MTU1010 following partially desiccation procedure. We studied the effects of several plant growth regulators on the efficiency of inducing embryogenic callus and regeneration of whole plantlets. The MS-CIM medium with 2.0 mg-1 2,4, D showed the highest callus induction percentage. Maximum rate of shoot regeneration (92%) was observed from 48hr partially dehydrated calli, when enriched with high cytokinin (2 mg/l kinetin) and lower auxin (0.5 mg/l NAA) concentrations. Calli that had been moderately dehydrated showed significantly greater regeneration rates than undehydrated calli. Upon being transferred onto a rooting medium, the well-rooted plantlets were shifted to shaded glass house for hardening. Hence, this approach is rather simple to apply and can be employed in genetic modification studies alongside other biotechnological endeavours.