Effect Of Light Intensity Research Articles

Warming induced shade tolerance to become a key trait in invasion success of free-floating plant Pistia stratiotes over the native Hydrocharis morsus-ranae

Changes in nutrient concentration, temperature and light intensity due to climate change can alter the species composition of aquatic ecosystems, since global climate change can intensify the process of eutrophication. Eutrophication can enhance the biological invasion and the distribution of alien aquatic plants. Here we investigated the competition ability of alien Pistia stratiotes and native Hydrocharis morsus-ranae and the effect of different light intensities, temperatures and nutrient concentrations on the functional traits of the two species. In short term (8 days) monoculture experiment, we applied low (0.5 mg L-1 N; 0.05 mg L-1 P) and high nutrient concentrations (2 mg L-1 N; 0.2 mg L-1 P), four different light intensities (25–295 μmol m−2 s−1) as well as cold and warm (21.5; 27.5 ± 0.5°C) water treatments in full factorial design. In mixed cultures we cultivated the plants for 28 days with various biomass ratio, in shaded and well illuminated conditions, at a high nutrient concentration (4 mg L-1 N 1 mg L-1 P). In monocultures, the relative growth rate of P. stratiotes in warm water was significantly higher than that of H. morsus-ranae, however, this difference was not significant in colder water. In the co-culture experiment, P. stratiotes had significantly higher growth rate compared to H. morsus-ranae regardless of initial plant biomass ratio. Under shaded (65 ± 5 μmol m−2 s−1) conditions, P. stratiotes outcompeted H. morsus-ranae, resulting in its decay. Experimental results imply that with elevated temperature, the spread of alien P. stratiotes can be expected. Furthermore, under shaded conditions, P. stratiotes has a higher chance of occupying the water surface over the native plant H. morsus-ranae.

The Effect of Light Intensity on the Chlorogenic Acid Biosynthesis Pathway in Marsdenia tenacissima

The goal of this study was to understand the effect of light intensity on the chlorogenic acid content and biosynthesis-related gene expression in Marsdenia tenacissima. In this study, M. tenacissima plants were treated with different light intensities; the chlorogenic acid content was determined by high-performance liquid chromatography; and transcriptome sequencing was performed. The amount of chlorogenic acid in the control was the highest and differed significantly from that under three different shading treatments. With a decrease in light intensity, the content of chlorogenic acid also showed a decreasing trend. A total of 1149 differentially expressed genes were identified by transcriptome sequencing, and most of the genes were down-regulated under the 90% shading treatment. A weighted gene co-expression network analysis identified the differentially expressed genes associated with light-induced chlorogenic acid biosynthesis. The different shading treatments down-regulated the expression of the chlorogenic acid biosynthesis pathway structural genes (HCTs). The MIKC family genes were the main transcription factors regulating light-induced chlorogenic acid biosynthesis, but the MYB and SBP family genes were also involved. In summary, combined physiological and transcriptome analysis, candidate structural genes, and transcription factors in the biosynthesis pathway of chlorogenic acid were identified in M. tenacissima.

Effect of light intensity on nanoparticle nucleation during printing of silver micropatterns via superluminescent light projection

Effect of light intensity on nanoparticle nucleation during printing of silver micropatterns via superluminescent light projection

Propagation light intensity influences yield, morphology, and phytochemistry of purple-leaf butterhead lettuce (Lactuca sativa)

Lettuce (Lactuca sativa) is an economically important leafy green produced in controlled environment agriculture (CEA). Optimization of lighting conditions is a crucial factor for sustainable production of high-quality lettuce in CEA systems. This study aimed to determine the effects of propagation light intensity (60, 100, 200, 400, and 600 μmol m−2 s−1, 24-h photoperiod) on harvestable yield, morphology, carotenoids, chlorophylls, and anthocyanins of purple-leaf butterhead 'Teodore' lettuce at transplant (14 days after showing) and harvest (21 days after transplanting in a greenhouse environment). We observed a 275 % increase in shoot fresh mass in seedlings grown under 600 μmol m−2 s−1 compared to those under 60 μmol m−2 s−1, and a 163 % improvement persisted through harvest. The HPLC quantification demonstrated that all carotenoids, including violaxanthin, neoxanthin, zeaxanthin, antheraxanthin, lutein, α-carotene, and total carotenoid content and/or concentration increased in lettuce seedlings as light intensities increased from 60 to 400 or 600 μmol m−2 s−1 (5.4–35.9 or 52.7 mol m−2 d−1 DLI). A similar increasing trend was also observed for chlorophyll accumulation. Anthocyanin concentration and content increased with increasing propagation light intensity, but no differences persisted through harvest. Overall, differences in most phytonutrient concentrations and contents persisted through harvest; carotenoid content trends were similar to those at transplant, but carotenoid and chlorophyll concentration trends were somewhat opposite with quadratic decreases as light intensity increased. The results indicate that propagation light intensity could be used as an effective and sustainable strategy to improve the harvestable yield and nutritional quality of lettuce grown in CEA.

The Cultivation of Spirulina maxima in a Medium Supplemented with Leachate for the Production of Biocompounds: Phycocyanin, Carbohydrates, and Biochar

Cyanobacteria are microorganisms that grow rapidly in an aquatic medium, showing the capacity of accumulations of biocompounds subsequently converted into value-added biocompounds. The cyanobacterium Spirulina maxima can produce pigments besides accumulating significant amounts of carbohydrates and proteins. An alternative to reducing biomass production costs at an industrial scale is the use of landfill leachate in the growing medium, as well as the mitigation of this pollutant. The objective of this work was to cultivate Spirulina maxima in a medium supplemented with leachate, using the design of experiments to evaluate the effects of leachate concentration (% v/v), light source, and light intensity in an airlift photobioreactor, analyzing them as a response to the productivity of biomass, phycocyanin, carbohydrates, and biochar. The highest values of productivity (mg L−1d−1) were 97.44 ± 3.20, 12.82 ± 0.38, 6.19 ± 1.54, and 34.79 ± 3.62 for biomass, carbohydrates, phycocyanin, and biochar, respectively, adjusted for experiment 2 with the factors of leachate concentration (5.0% v/v), light source (tubular LED), and luminosity (54 µmol m−2 s−1), respectively. The use of leachate as a substitute for macronutrients in Zarrouk’s medium for the cultivation of Spirulina maxima is a viable alternative in the production of biocompounds as long as it is used at an appropriate level.

Gene expression regulation of the effect of shading on chlorophyll content in Fuding White Tea (Camellia sinensis L.).

Shading is an important practical method to improve the quality of green tea. Previous research of our group found that because the biosynthesis and distribution of theanine in tea plants were affected by down regulation of gene encoding amino acid permeases, theanine content in tea leaves which grown under shading condition was significantly higher than those under natural light. In this study, our group analyzed the changes of tea leaf area, free amino acid content and photosynthetic parameters under natural light and shading conditions, to ensure that moderate shading did not reduce but improve the quality of tea. Transcriptome sequencing and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were conducted to reveal the expression levels of genes related to chlorophyll content and chlorophyll a/b ratio under natural light and shading conditions. Experimental results revealed the presence of the following differentially expressed genes (DEGs) in the porphyrin and chlorophyll metabolism pathway of tea under natural light and shading conditions: the up regulation of CPOX expression may lead to an increase in the accumulation of raw materials of chlorophyll synthesis, while the down regulation of SGR expression may lead to a decrease in chlorophyll degradation. The combined effect of these two genes may lead to an increase in the total chlorophyll content of tea. The down regulation of NOL expression may lead to the obstruction of chlorophyll b transform to chlorophyll a, that is, the decrease of the chlorophyll a/b ratio. This study investigated the molecular mechanism of chlorophyll content and component alteration in Fuding white tea under natural light and shading conditions, and elucidated the effects of different light intensities on the porphyrin and chlorophyll metabolism pathway of tea. Thus provided deep understanding of chlorophyll regulation under shading condition in tea cultivation, which could contribute to high-quality matcha production.

Photoacclimation and photophysiology of four species of toxigenic Dinophysis

This study aimed to explore the effects of different light intensities on the ecophysiology of eight new Dinophysis isolates comprising four species (D. acuminata, D. ovum, D. fortii, and D. caudata) collected from different geographical regions in the US. After six months of acclimation, the growth rates, photosynthetic efficiency (Fv/Fm ratio), toxin content, and net toxin production rates of the Dinophysis strains were examined. The growth rates of D. acuminata and D. ovum isolates were comparable across light intensities, with the exception of one D. acuminata strain (DANY1) that was unable to grow at the lowest light intensity. However, D. fortii and D. caudata strains were photoinhibited and grew at a slower rate at the highest light intensity, indicating a lower degree of adaptability and tolerance to such conditions. Photosynthetic efficiency was similar for all Dinophysis isolates and negatively correlated with exposure to high light intensities. Multiple toxin metrics, including cellular toxin content and net production rates of DSTs and PTXs, were variable among species and even among isolates of the same species in response to light intensity. A pattern was detected, however, whereby the net production rates of PTXs were significantly lower across all Dinophysis isolates when exposed to the lowest light intensity. These findings provide a basis for understanding the effects of light intensity on the eco-physiological characteristics of Dinophysis species in the US and could be employed to develop integrated physical-biological models for species and strains of interest to predict their population dynamics and mitigate their negative effects.

A novel combined method for nitrate removal by chemical Pd–Cu/alumina bimetallic catalyst and biohydrogen produced by Rhodobacter johrii VT1 in an anaerobic, photo-fermentation process

In this study, biohydrogen was produced by R. johrii strain VT1 using an anaerobic photo-fermentation process on low-cost industrial wastewater and then used for nitrate reduction in the presence of a chemical catalyst. The effect of substrate, light intensity, and pH on biohydrogen production was investigated. For efficient nitrate reduction, a Pd–Cu/alumina bimetallic catalyst was prepared and analyzed by XRD, FTIR, FESEM, and ICP. Accumulative biohydrogen production was 284.67 ml/h (0.409 ± 0.005 mol/m3h), with molasses, a light intensity of 5300 lux, and a pH of 9. Nitrate removal efficiency was 81.70%, and nitrogen selectivity was 94.25%. The XRD results also showed the stability of the catalyst structure during the reduction. Our study shows that this combined method is effective for nitrate removal and has the potential for large-scale work because of the affordable and sustainable green hydrogen production from wastewater. This is the first report of using biological hydrogen for harmful nitrate removal from drinking water in the presence of a chemical catalyst.

Optoelectronic properties of Au/n-type Si semiconductor structures with SiO2 interlayer

In this study, we investigated the effect of light intensity on electrical and photovoltaic properties using current-voltage (I–V) measurements at room temperature of Au/n-type Si structures with SiO2 interface layer. At each light intensity, the obtained main electrical characteristics such as ideality factors (n), barrier heights (Φbo), rectification ratios (RR), saturation currents (I0) and series resistances (RS) of Au/SiO2/n-type Si structures were calculated from both thermionic emission (TE) and Cheung methods using I–V measurements and compared to each other. Experimental results showed that while the n and I0 values increased with the increase in the light intensity, the Φbo and RR values decreased. While the ideality factor values obtained using the TE method ranged between 1.973 (at dark) and 2.250 (for 100 mW/cm2), these values were obtained between 3.227 and 3.482 using the Cheung method. At the same time, while the barrier height values obtained using the TE method ranged between 0.865 eV (at dark) and 0.817 eV (for 100 mW/cm2), these values were obtained between 0.773 eV and 0.742 eV using the Cheung method. It was observed that while the n values obtained from TE theory were smaller than the values obtained from the Cheung method, the Φbo values were larger than the values obtained from the Cheung function. Furthermore, for the Au/SiO2/n-type Si structures, the photovoltaic parameters such as short circuit current (Isc) and open circuit voltage (Voc) and photocurrent (Iph) were examined under dark, daylight and various light intensities (from 20 to 100 mW/cm2).

Performance test of Zn-astaxanthin complex-sensitized solar cell: effect of light intensity on open-circuit voltage and short-circuit current values.

The sensitizer is one of the most essential dye-sensitized solar cell (DSSC) components. In the present research, a Zn-astaxanthin complex was investigated as a sensitizer, compared to pure astaxanthin. The complex with a 1:1 mole ratio between astaxanthin and Zn2+ was synthesized in a reflux reactor at 37-60 °C. The product was analyzed using Proton Nuclear Resonance (1H-NMR), which indicates the presence of chelate formation between Zn2+ with two atoms of oxygen on the terminal cyclohexane ring of astaxanthin. The interaction of sensitizers (astaxanthin and Zn-astaxanthin) on the photoelectrode surface in this study was analyzed using a Fourier Transform Infra-Red (FTIR) and Ultraviolet-Visible Diffuse Reflectance Spectroscopy (UV-Vis DRS). The FTIR spectra of photoelectrode immersed in Zn-astaxanthin show peaks of C=O stretching and vibration -OH group at 1730 and 1273 cm-1, respectively, and H-C-H stretching vibration with high intensity in 2939, 2923, and 2853 cm-1. The UV-Vis DRS analysis shows the band gap of photoelectrode (PE), photoelectrode immersed in astaxanthin (PE/astaxanthin), and Zn-astaxanthin (PE/Zn-astaxanthin) are 3.19, 1.65, and 1.59 eV, respectively. Under illumination intensity of 300 W/m2, the maximum energy conversion efficiency of DSSC with Zn-astaxanthin as sensitizer is (0.03 ± 0.0022)%, higher than DSSC with astaxanthin as sensitizer ((0.12 ± 0.0052)%). Up to 70 h of illumination, DSSC with Zn-astaxanthin as a sensitizer also has better stability than astaxanthin-based DSSC.

Light intensity differentially mediates the life cycle of lepidopteran leaf feeders and stem borers.

Leaf feeders, such as Spodoptera frugiperda and Spodoptera litura, and stem borers Ostrinia furnacalis and Chilo suppressalis, occupy two different niches and are well adapted to their particular environments. Borer larvae burrow and inhabit the interior of stems, which are relatively dark. By contrast, the larvae of leaf feeders are exposed to sunlight during feeding. We therefore designed series of experiments to evaluate the effect of light intensity (0, 2000, and 10 000 lx) on these pests with different feeding modes. The development of all four pests was significantly delayed at 0 lx. Importantly, light intensity affected the development of both male and female larvae of borers, but only significantly affected male larvae of leaf feeders. Furthermore, the proportion of female offspring of leaf feeders increased with increasing light intensity (S. frugiperda: 33.89%, 42.26%, 57.41%; S. litura: 38.90%, 51.75%, 65.08%), but no significant differences were found in stem borers. This research also revealed that the survival rate of female leaf feeders did not vary across light intensities, but that of males decreased with increasing light intensity (S. frugiperda: 97.78%, 85.86%, 61.21%; S. litura: 95.83%, 73.54%, 58.99%). These results improve our understanding of how light intensity affects sex differences in important lepidopteran pests occupying different feeding niches and their ecological interactions with abiotic factors in agroecosystems. © 2024 Society of Chemical Industry.

Effect of light intensity on photophysiology and growth dynamics of crustose coralline algae (CCA): implications for the loss of canopy-forming algae

Effect of light intensity on photophysiology and growth dynamics of crustose coralline algae (CCA): implications for the loss of canopy-forming algae

Effect of light intensity on the survival of European spiny lobster (Palinurus elephas) larvae reared in aquaculture system

Light plays a crucial role in the growth, development, and survival of aquatic organisms. To evaluate the survival of European spiny lobster (Palinurus elephas) larvae in relation to different light intensities (26, 359, and 910 lux, equivalent to 0.35, 4.74, and 12.29 μmol·m−2·s−1, respectively), this study monitored the survival rates of reared spiny lobster from the early instar phyllosoma stages to their metamorphosis into the puerulus stage (111 days after hatching) at the three light intensities. The newly hatched larvae were cultured in 65-L Kreisel tanks with 200 individuals each (three replicates per treatment, i.e., light intensity). Phyllosoma stages I to III larvae showed no significant differences in survival rates under different light intensities. However, light intensity did have a significant impact on the survival rates of phyllosoma stages IV and V as well as the metamorphosis of phyllosoma VI into puerulus. The highest survival rates were observed under high and medium light intensity conditions. At the end of the experiment, the highest mean survival rate for stage VI, the metamorphosis into the puerulus stage, was 42.0 ± 2.52 % under high light intensity. These survival rates for reared pueruli were 84 times higher than those achieved in previous studies. The present study results show promise for the successful implementation of commercial scale rearing protocols for European spiny lobster and have implications for the design of crustacean larvae culture systems.

Effects of different light intensity on leaf color changes in a Chinese cabbage yellow cotyledon mutant.

Leaf color is one of the most important phenotypic features in horticultural crops and directly related to the contents of photosynthetic pigments. Most leaf color mutants are determined by the altered chlorophyll or carotenoid, which can be affected by light quality and intensity. Our previous study obtained a Chinese cabbage yellow cotyledon mutant that exhibited obvious yellow phenotypes in the cotyledons and the new leaves. However, the underlying mechanisms in the formation of yellow cotyledons and leaves remain unclear. In this study, the Chinese cabbage yellow cotyledon mutant 19YC-2 exhibited obvious difference in leaf color and abnormal chloroplast ultrastructure compared to the normal green cotyledon line 19GC-2. Remarkably, low-intensity light treatment caused turn-green leaves and a significant decrease in carotenoid content in 19YC-2. RNA-seq analysis revealed that the pathways of photosynthesis antenna proteins and carotenoid biosynthesis were significantly enriched during the process of leaf color changes, and many differentially expressed genes related to the two pathways were identified to respond to different light intensities. Remarkably, BrPDS and BrLCYE genes related to carotenoid biosynthesis showed significantly higher expression in 19YC-2 than that in 19GC-2, which was positively related to the higher carotenoid content in 19YC-2. In addition, several differentially expressed transcription factors were also identified and highly correlated to the changes in carotenoid content, suggesting that they may participate in the regulatory pathway of carotenoid biosynthesis. These findings provide insights into the molecular mechanisms of leaf color changes in yellow cotyledon mutant 19YC-2 of Chinese cabbage.

Effect of Far-Red Light on Biomass Accumulation, Plant Morphology, and Phytonutrient Composition of Ruby Streaks Mustard at Microgreen, Baby Leaf, and Flowering Stages.

Far-red (FR) light influences plant development significantly through shade avoidance response and photosynthetic modulation, but there is limited knowledge on how FR treatments influence the growth and nutrition of vegetables at different maturity stages in controlled environment agriculture (CEA). Here, we comprehensively investigated the impacts of FR on the yield, morphology, and phytonutrients of ruby streaks mustard (RS) at microgreen, baby leaf, and flowering stages. Treatments including white control, white with supplementary FR, white followed by singularly applied FR, and enhanced white (WE) matching the extended daily light integral (eDLI) of FR were designed for separating the effects of light intensity and quality. Results showed that singular and supplemental FR affected plant development and nutrition similarly throughout the growth cycle, with light intensity and quality playing varying roles at different stages. Specifically, FR did not affect the fresh and dry weight of microgreens but increased those values for baby leaves, although not as effectively as WE. Meanwhile, FR caused significant morphological change and accelerated the development of leaves, flowers, and seedpods more dramatically than WE. With regard to phytonutrients, light treatments affected the metabolomic profiles for baby leaves more dramatically than microgreens and flowers. FR decreased the glucosinolate and anthocyanin contents in microgreens and baby leaves, while WE increased the contents of those compounds in baby leaves. This study illustrates the complex impacts of FR on RS and provides valuable information for selecting optimal lighting conditions in CEA.

Irradiating roots of komatsuna (Brassica napus) with various light qualities affects growth and nutrient content in leaves, stems, and roots

Generally, plants grow without light exposure to their roots. Although the effects of light intensity and irradiation at different wavelengths on plant growth and composition in leaves and stems are becoming clearer, the effects of wavelength irradiation on roots remain largely unknown. Therefore, in this study, the effects of root irradiation with seven light wavelengths on the growth and ion and vitamin contents in hydroponically-grown komatsuna (Brassica napus) were investigated. Compared to the control treatment, the 940-nm treatment significantly increased root dry matter weight, whereas the other treatments exhibited no effect on leaves, stems, or roots. At specific irradiation wavelengths, the ion content of potassium, magnesium, calcium, boron, manganese, and zinc in the leaves and stems increased significantly compared to that in the control. The changes in ion contents in the leaves and stems were consistent with those in the roots, except for boron. Furthermore, regarding vitamins, the contents of nicotinic acid, pyridoxine, riboflavin, ascorbic acid, and phylloquinone in the leaves and stems increased significantly under irradiation compared with the control. Trends in the respective vitamin contents in leaves and stems did not coincide with those in roots. In some cases, irradiation significantly decreased the ion and vitamin contents compared to the control. These results indicate that a photoreceptor mechanism exists in roots, leaves, and stems and that irradiation of roots at different wavelengths affects the growth and nutrient content of komatsuna.

Study of the Structural and Switching Properties of Ga2Te5 Crystals

The structural parameters of Ga2Te5 crystals grown using the Bridgman method have been studied. X-ray diffaction analysis revealed the crystal structure of Ga2Te5 in the tetragonal phase. In addition, crystalline size, strain, and dislocation density were calculated with the Sherrer model and the Williamson-Hall (W-H) model. The switching effect was achieved for Ga2Te5 crystals. Furthermore, the effect of temperature and light intensity was studied for Ga2Te5 crystals. The results show that temperature and light intensity affect switching characteristics such as threshold current (ith), threshold voltage (Vth), threshold power (Pth), and resistance ratios from a high-resistance OFF state to a low-resistance ON state (ROFF/RON).

The Effect of Light-emitting Diode Light Intensities and Duration of Cure on Pulpal Wall Temperature among Different Classes of Teeth.

The thermal changes during light curing of resin-based composites (RBCs) are recognized, but there is a lack of information about temperature changes in pulp tissue under different curing protocols, especially during bonding of orthodontic brackets onto the tooth surface. In the present study, pulpal temperature (PT) variation induced by the use of four different light cure intensities and different durations of cure among different classes of teeth was measured. A total of 80 different classes of human extracted teeth (maxillary and mandibular central incisor, lateral incisor, canine, premolar, and molar) were irradiated at the same distances with four visible light curing intensities. Brackets (3M Unitek) were bonded with Transbond XT (3M Unitek, Monrovia, California) adhesive and light cured with a light-emitting diode (LED) light cure unit (LCU) (Bluephase). A J-type thermocouple wire was positioned in the center of the pulp chamber to assess the temperature difference. The results were analyzed with analysis of variance (ANOVA) and the Tukey's honestly significant difference (HSD) test. Light-emitting diode with higher intensity induced significantly higher intra-PT changes than did the LED with lower intensity (p < 0.001). Mandibular central incisors had the highest intra-PT of about 45°C at 3000 mW/cm2, 3 seconds. Maxillary central incisors had the highest intra-PT of about 40°C at 3000 mW/cm2, 3 seconds. Mandibular and maxillary canines and molars had the lowest intra-PT of about 10°C at 800 mW/cm2, 20 seconds. Pulp chamber temperature changes were influenced by the intensity and duration of curing and the anatomy of the tooth (class/type of tooth). S S, Anbarasu P, S SK, et al. The Effect of Light-emitting Diode Light Intensities and Duration of Cure on Pulpal Wall Temperature among Different Classes of Teeth. Int J Clin Pediatr Dent 2024;17(4):467-471.

Development of Photovoltaic Power Plant Prototype as a Learning Media on The Subject of Renewable Energy

Renewable Energy needs to be implemented in schools to build students' knowledge. This research aims to design a prototype of a Photovoltaic Power Plant (PLTS), which can be used as a medium for experimental high school physics activities. Besides that, the effect of light intensity on the power produced by the PLTS prototype was experimentally tested by observing temperature, humidity, and air pressure. The PLTS prototype is designed to be simple, with component selection (10 wp solar panels, SCC, batteries, lights and panel boxes) that are adapted to the learning media criteria, namely practical, durable, easy to use and able to demonstrate the concepts being taught. The PLTS prototype was tested when charging and discharging the battery. When charging the battery, the solar panel is tilted with a variety of tilt angles of 0º, 15º, 30º, 45º and 60º. When discharging the battery, lamps with varying powers are used, namely 3W, 5W, 7W and 9W. The research results show that the panel tilt angle is used to produce maximum voltage, current, and power when charging the battery, which is 30°. When the battery is discharged, a smaller lamp power can light the lamp for longer compared to a larger lamp power. In conclusion, the PLTS prototype can provide students with experience in conducting experiments and learning the application of physics concepts directly so that it can build students' knowledge and provide meaningful learning.

Box-Behnken Design for Photocatalytic Degradation of Sudan Black B by Catalyst-Embedded Multiwalled Carbon Nanotubes

This research employs a Box Behnken design-based technique for photocatalytic use of Multiwalled Carbon Nanotubes (MWCNTs) derived from spray pyrolysis. This procedure was used for photocatalytic degradation applications on Sudan black dye using Citrus limonum oil as a carbon precursor and Fe/Co/Mo supported on silica as a catalyst. The influence of initial dye concentration was compared to the effects of H2O2, catalyst concentration and solar light intensity. The Response Surface Methodology was used to optimize growth parameters for higher yield and graphitization. The as-grown CNTs were exemplified using scanning and transmission electron microscopy and Raman spectroscopy. This work resulted in the identification of the optimal set of spray pyrolysis turning parameters for achieving high CNT yield and effective decolorization of Sudan black B dye by employing MWCNTs as catalyst. The degree of decolorization of Sudan black B dye increases upto a certain level with an increase in initial H2O2 concentration and subsequently declines as excess H2O2 hydroxyl radicals formed serve as scavengers, resulting in less dye decolonization.