Effect Of Green Tea Extract Research Articles

Green tea improves rat sperm quality and reduced cadmium chloride damage effect in spermatogenesis cycle.

Introduction: Testicular tissue is part of the reproductive system that some mineral compounds such as cadmium chloride (CdCl2) destroy. Green tea (Camellia sinensis) extract can reduce the tissue damage caused by toxins due to its antioxidant properties. The aim of this study was to evaluate the effect of green tea extract on sperm quality in cadmium chloride toxicity.Materials and Methods: In the present study, male Wistar rats were allotted randomly into four groups, namely control group (C), CdCl2 (1.5mg/kg), GT 1.5% (w/v) and in combinationCdCl2+GT groups. CdCl2 was injected intraperitoneally (1.5 mg /kg) whereas the green tea extract was administrated orally. At 13, 25 and 49 days after treatment, the rats were euthanized and the reproductive organs (testes, epididymis) were excised and used for sperm analysis and histo-morphometric examinations.Results: The mean of the diameter of seminiferous tubes, the number of spermatogonia, Sertoli, Leydig cells and thickness of the germinal layer in the testis were significantly increased (P<0.05) in all groups compared to the CdCl2 group (P<0.05). Sperm motility, sperm count and testosterone were significantly decreased in the CdCl2 group compared to all groups of treatment (p<0.05). The mean of MDA was significantly increased in the CdCl2 group compared to other groups (p<0.05).Conclusion: Green tea has an antioxidant effect that reduces the effects of free oxygen radicals produced from toxins such as cadmium chloride. In addition, it could decrease lipid peroxidation of the cell membrane and ultimately prevent the destruction of tissues in the long run.

Hepatoprotective Effect of Green Tea Extract against Cyclophosphamide Induced Liver Injury in Albino Rats

Background: Green tea intake is accompanied with a lower incidence of cardiovascular disease, cancer and neurodegenerative disorders; hence green tea extract has been included as dietary supplement along with other supplements and multivitamins. Aim of the Work: Studying the effect of cyclophosphamide administration on the liver of adult male albino rats and the possible protective role of green tea extract. Material and Methods: The current study was carried out on 45 adult male albino rats. They were divided into three equal groups (each included 15 rats). Group I (control group) was injected intraperitoneally with normal saline at a dosage of 0.5 mg/kg body weight twice weekly for 9 weeks. Group II was injected intraperitoneally with cyclophosphamide (CP) (150 mg/kg/day) for two weeks. Group III: rats received green tea extracts orally (50 mg/kg/day) for three weeks, and then continued for further two weeks concomitantly with intraperitoneally cyclophosphamide (CP) injected (150 mg/kg/day). Results: Rats exposed to cyclophosphamide (CP) showed several histological and histochemical changes in their liver. These changes were improved by using green tea. Conclusion: The present work showed that green tea had preventive and therapeutic effect upon livers of albino rats after they were exposed to CP.

Thiocyclam-Induced Reproductive Toxicity, Oxidative Stress and Genomic DNA Damage in Testicular Tissues of Rats: Protective Effects of Green Tea Extract

Thiocyclam is a broad-spectrum nereistoxin analogue insecticide which widely used for agricultural applications. The aim of this study is to investigate the protective effects of green tea (as a principal source of antioxidants) on Thiocyclam-Induced Reproductive Toxicity, Oxidative Stress and Genotoxicity in adult Male Rats. Forty adult male rats were randomly assigned into four groups: control, Thiocyclam (ThC, 15.98 mg/kg b.w.), green tea extract (GTE, 2% w/v as the sole beverage) and (ThC+GTE) group. After 65 days of treatment, blood samples and testicular tissue were collected for measuring the oxidative stress parameters, testosterone level and DNA damage, whereas the reproductive organs were weighed, and Semen analysis and testicular histopathological studies was done. The results revealed that Thiocyclam administration induce a significant reduction in sperm count, sperm motility as well as testosterone level, while increased sperm abnormality. In addition to increased testicular tissue Malondialdehyde (MDA), reduced GSH content and testicular DNA damage evidenced by comet assay. The histological examination of testes revealed degenerative change and disorganization of seminiferous tubules with incomplete spermatogenesis. On the contrary, GTE played a potential protective effect against Thiocyclam induced oxidative stress as well as alleviate the reproductive toxicity by improving the oxidative status, decrease oxidative DNA damage, improving semen characteristics and protective effect against testicular damage, highlighting the protective and therapeutic potentiality of green tea against pesticide-induced reproductive toxicity and its health benefits.

Effects of Green Tea Extracts on freshwater angelfish, Pterophyllum scalare Growth Performance

The study was conducted to investigate the effects of dietary green tea extract (GTE) supplementation in diet on growth performance, feed utilization and biometric indexes in freshwater angelfish, Pterophyllum scalare. The fish (mean body weight, 2.61±0.01 g) were fed fish meal diets that included 0% (control), 2.5 and 5% GTE for 8 weeks. The results showed, the addition of green tea extract did not have a positive effect on growth performance and other variables. Addition of green tea extract did not change the amount of viscerosomatic index and hepatosomatic index.

Effects of Antioxidants Supplement in Porcine Sperm Freezing on in vitro Fertilization and the Glutathione and Reactive Oxygen Species Level of Presumptive Zygotes

The present study was aimed to determine the effects of green tea extract (GTE) and beta-mercaptoethanol (β-ME) supplementation in boar sperm freezing extender on in vitro fertilization (IVF) and reactive oxygen species (ROS) and glutathione (GSH) levels of presumptive zygotes (PZs). Experimental groups were allocated into lactose egg yolk (LEY) without antioxidant (control), GTE (1,000 mg/l in LEY) and β-ME (50 μM in LEY). In freezing, spermatozoa extended with LEY were cooled to 5°C for 3 h and then kept at 5°C for 30 min following dilution with LEY containing 9% glycerol and 1.5% Equex STM. The final sperm concentration was 1 × 108/ml. Spermatozoa were loaded into straws and frozen in nitrogen vapor for 20 min. For IVF, oocytes were matured in NCSU-23 medium and co-cultured with spermatozoa following thawing at 37°C for 25 sec. At 12 h following IVF, IVF parameters (sperm penetration and monospermy) were evaluated. In addition, GSH and ROS levels of PZs were determined by Cell Tracker Blue CMF2HC and DCHFDA, respectively. IVF parameters did not show any significant difference among the experimental groups. GSH and ROS levels of PZs were not significantly different between groups. In conclusion, antioxidant supplementation in boar sperm freezing could not influence IVF parameters, ROS and GSH levels of PZs.

Effect of green tea extract on bone mass and body composition in individuals with diabetes

Green tea has shown benefits in diabetes and osteoporosis. The purpose of this article is to evaluate the effect of green tea on glycemic control, body composition and bone mass in diabetic subjects. A double-blind, randomized, controlled clinical trial was conducted with 35 diabetic subjects. Participants received 1120 mg of green tea extract containg 560 mg of polyphenols/day or cellulose. Body composition, bone mass measures and other parameters are evaluated after 10 and 20 weeks. For bone mineral content, a 1.60 g increase in green tea group and a 0.98 g decrease in control group resulted in a difference in mean of 2.58 g between groups approaching significance (p = 0.056). It was observed an increase in PTH after 10 weeks in the green tea group (p = 0.008) with a subsequent reduction at 20 weeks. Thus, green tea may contribute to improvement of bone mineral content in diabetic subjects.

Modulatory effects of green tea extract against the hepatotoxic effects of 4-nonylphenol in catfish (Clarias gariepinus)

The antioxidant role of the green tea (Camellia sinensis) extract (GTE) was examined to remedy the toxic effects of (0.2mgl−1) 4-nonylphenol(4-NP). Biochemical parameters, antioxidant enzymes, liver lipid peroxidation (LPO), DNA fragmentation, and apoptosis as well as histopathology of liver of African catfish Clarias gariepinus were considered. Catfishes were divided into four groups: first group (control), second group (0.2mgl−1 of 4-NP), third group (0.2mgl−1of 4-NP +100mg GTE l−1water), and fourth group (0.2mgl−1 of 4-NP +200mg GTE l−1water). The results showed that significant increments of serum glucose, AST, ALT, total protein, total lipids, cholesterol, G6PDH, and cortisol. Meanwhile, serum acetylcholinesterase, ALP, and LDH were significantly reduced. In addition, antioxidant enzymes (SOD, CAT, GST, and TAC) levels were reduced in 4-NP treated fish compared to control. Also, there were significant increments in hepatic LPO, DNA fragmentation, and apoptotic erythrocytes in 4-NP treated fish compared to control. Liver of 4-NP treated fish showed some histopathological alterations such as, vacuolization in hepatocytes, congestion in central vein, infiltration of mononuclear inflammatory cells, and necrosis as well as depletion of glycogen content of liver. Addition of green tea extract into the water restored the alterations in most of those biomarkers induced by 4-NP. We concluded that, GTE has a protective role against hepatic failure, depletion of antioxidant defense, and genotoxicity induced 4-NP in C. gariepinus.

Effect of subchronic intake of green tea extract on liver of albino rat histomorphometric, ultrastructural and biochemical study.

There are conflicting reports on the effect of green tea extract (GTE) on the liver of animals. Some studies failed to show any adverse hepatic effects following administration of GTE to mice, rats, and dogs. Others reported severe hepatic necrosis, resulting in death in female Swiss-Webster mice following its administration. The aim of the study was to examine the subchronic toxicity of GTE on the liver of the adult male albino rats. Forty male adult Wistar albino rats were used in the study. The rats were divided into four groups; group I (control), group II (low-dose green tea), group III (medium-dose green tea) and group IV (high-dose green tea). Histological, biochemical and histomorphometric analyses were done. Mild hepatic affections were observed in group II. The affections were severe in groups III and IV. The central veins and hepatic sinusoids were congested. The hepatocytes were degenerated. Hypertrophy of the hepatic arteries, dilation of the bile ducts and cellular infiltration were clearly observed in the last two groups. Mild degenerative changes were observed in the hepatocytes in rat's group II; the cytoplasm was rarefied and vacuolated. Some mitochondria were ruptured. The blood sinusoids were congested. The rough endoplasmic retinaculum was fragmented in group III. More degenerative changes were observed in group IV; the hepatic architectures were lost with disruption of cell membranes. Most of the cell organelles were degenerated and most of mitochondria were ballooned. As compared to that of the control groups: the total serum protein values in groups II, III and IV showed a statistically significant decrease (12%, 20% and 21%, respectively), the mean area per cent of collagen fibres in groups III and IV increased 5 and 7 folds. Subchronic administration of GTE resulted in structural and functional affection of the rats' liver. The dose of 250 mg/kg/day seemed to be safe, while the doses of 500 mg/kg/day and 1000 mg/kg/day had deleterious effect being more evident in the latter dose.

Long-Term Intake of Green Tea Extract Causes Mal-Conformation of Trabecular Bone Microarchitecture in Growing Rats.

The purpose of this study was to examine the effects of green tea extract (GTE) intake on bone structural and physiological properties, such as bone mass, trabecular bone microarchitecture, cortical bone geometry, and bone mechanical strength, in growing rats. Four-week-old male Wistar rats were divided into the following four groups: standard diet feeding for 85days (S-CON) or 170days (L-CON), and GTE diet feeding for 85days (S-GTE) or 170days (L-GTE). At the end of the experiment, in addition to measurement of circulating bone formation/resorption markers, bone mass, trabecular bone microarchitecture, and cortical bone geometry were analyzed in the left femur, and bone mechanical strength of the right femur was measured. There was no difference in all bone parameters between the S-CON and S-GTE groups. On the other hand, the L-GTE group showed the decrease in some trabecular bone mass/microarchitecture parameters and no change in cortical bone mass/geometry parameters compared with the L-CON group, and consequently the reduction in bone weight corrected by body weight. There was no difference in bone formation/resorption markers and bone mechanical strength between the S-CON and S-GTE groups and also between the L-CON and L-GTE groups. However, serum leptin levels were significantly lower in the L-GTE group than in the L-CON group. Thus, the long-term GTE intake had negative effects on bone, especially trabecular bone loss and microarchitecture mal-conformation, in growing rats.

The Effects of Green Tea Extract on Working Memory in Healthy Women.

This study aimed to examine the effects of green tea extract on working memory in healthy younger (21 - 29 y) and older (50 - 63 y) women. A single-blind, placebo-controlled, crossover design was used. A university laboratory. Twenty non-smoking Caucasian women were recruited in the younger (10) and older (10) age group. Subjects received 5.4 g green tea extract (at least 45% epigallocatechin-3-gallate) or placebo (cornstarch) within a 24-hour period. Working memory was measured by reading span and N-back task paradigm. Blood sample (20 mL) was collected and measured for plasma malondialdehyde (MDA) and total antioxidant capacity (TEAC) concentration. A 24-hour recall was conducted for each treatment period to ensure similar dietary patterns. Green tea extract significantly improved reading span performance in older women, indicated by higher absolute and partial scores of reading span. No significant changes were observed in the younger group. N-back latencies and accuracies were not significantly different after green tea treatment in either age group. Plasma concentration of MDA and TEAC were not different after green tea extract in either group. Acute supplementation of decaffeinated green tea extract may enhance working memory capacity of women between 50 to 63 years of age. This study provides preliminary evidence that consumption of green tea extract may enhance the cognitive performance in older adults and thus provide potential chemopreventive benefits in this group. The mechanism should be explored in future research.

Green tea extract outperforms metformin in lipid profile and glycaemic control in overweight women: A double-blind, placebo-controlled, randomized trial

Both green tea and metformin are used as adjuvants to treat and prevent complications associated with obesity; however, studies comparing their action and interaction in non-diabetic overweight women have not been reported. Thus, the current study evaluated the effects of green tea extract and metformin, both individually and in combination, on type 2 diabetes risk factors in non-diabetic overweight women. A total of 120 overweight women were randomly assigned in a double-blind manner to 1 of 4 groups, as follows: control (n=29; 1g of cellulose), green tea (n=32; 1g of dry green tea extract), metformin (n=28; 1g of metformin), and green tea+metformin (n=31; 1g of dry green tea extract+1g of metformin). Each group took the indicated capsules daily for 12 weeks. Anthropometric measurements, body composition, and fasting blood samples were evaluated. Although no significant interactions were observed in glycaemic control (p=0.07), green tea in the absence of metformin reduced fasting glucose (-4.428±2.00; p=0.031), but when combined the lowering effect was nullified. In contrast, metformin increased HbA1c concentration (0.048±0.189%; p=0.017) and also reduced body weight (-1.318±0.366kg; p=0.034) and LM (lean mass) (-1.249±0.310; p=0.009). Regarding lipid parameters, green tea significantly reduced total cholesterol and LDL-c. Green tea was superior to metformin in improving glycaemic control and lipid profile in non-diabetic overweight women and, therefore, green tea extract is a promising alternative for reducing type 2 diabetes risk in overweight women.

The effect of green tea extract as a matrix metalloproteinase inhibitor on the bond strength of resin composite

The effect of green tea extract as a matrix metalloproteinase inhibitor on the bond strength of resin composite

Effect of Green Tea Extract on Exercise-Induced Oxidative Stress in Obese Men: A Randomized, Double-Blind, Placebo-Controlled, Crossover Study

Background: Green tea contains polyphenol catechins that has been shown to be beneficial to health owing to its antioxidant properties. Objectives: This study investigated the effects of a 14-day ingestion of green tea extract (GTE) on biomarkers of oxidative stress in obese men undergoing resistance exercise (RE). Methods: In a randomized, double-blind, placebo (PL)-controlled, crossover study, ten obese men (age 37.12 ± 5.66 y; height 177 ± 7.7 cm; BMI 32.53 ± 2.52) performed two RE protocols consisting of 3 sets of 6 exercises to exhaustion at 75% of one repetition maximum (1RM) and 2 minute rests between sets, after 14 days of 500 mg of GTE (two capsules per day) or 500 mg PL (two capsules per day) supplementation. Blood samples were collected before (PRE) and immediately after (POST) RE and analyzed for oxidative biomarkers including lipid peroxidation (8-iso PGF2α) and oxidative DNA damage (8-OHdG). Results: Our results showed that a 14-day GTE supplementation did not change lipid peroxidation (P > 0.05); also, no significant alterations for 8-iso PGF2α between GTE and PL treatments after RE were observed (P > 0.05). However, 14-day GTE supplementation significantly reduced oxidative DNA damage as measured by 8-OHdG after RE compared to PL (P < 0.05). Conclusions: The results of this study demonstrated that 14-day supplementation of GTE significantly prevented oxidative DNA damage induced by RE in untrained obese men. However, the potential effects of GTE on oxidative stress after RE in obese people warrants further investigation.

A Randomized, Double-blind, Placebo-controlled Clinical Trial Examining the Effects of Green Tea Extract on Systemic Lupus Erythematosus Disease Activity and Quality of Life.

Antiinflammatory and immunomodulatory benefit of green tea (Camellia sinensis) in autoimmune disease has been proven in recent studies. The objective of this study was to assess the effects of green tea on disease activity and quality of life in systemic lupus erythematosus patients. A randomized controlled trial on subjects with lupus was conducted, and 68 patients in the age range of 39.1±10.3years and body mass index of 25.7±5.21kg/m2 completed the 12-week study. Patients were randomly divided into two groups of intervention (1000mg green tea extract, two capsules/day) and control (1000mg of starch, two capsules/day). Main outcome measure, systemic lupus erythematosus disease activity, was assessed by the systemic lupus erythematosus disease activity index at the first and after 3months of intervention. In addition, patient's quality of life was evaluated by short form of quality-of-life questionnaire at baseline and after 3months. Green tea extract supplementation significantly reduced disease activity in lupus patients (p<0.004); in addition, it significantly increased the vitality (p<0.006) and general health (p<0.01). This study showed that daily consumption of green tea extracts for 12weeks improves the systemic lupus erythematosus disease activity as well as some aspects of quality of life. Copyright © 2017 John Wiley & Sons, Ltd.

Effect of green tea extract on enteric viruses and its application as natural sanitizer

In this work, the effect of green tea extract (GTE) was assessed against murine norovirus (MNV) and hepatitis A virus (HAV) at different temperatures, exposure times and pH conditions. Initially, GTE at 0.5 and 5 mg/ml were individually mixed with each virus at 5 log TCID50/ml and incubated 2 h at 37 °C at different pHs (from 5.5 to 8.5). GTE affected both viruses depending on pH with higher reductions observed in alkaline conditions. Secondly, different concentrations of GTE (0.5 and 5 mg/ml) were mixed with viral suspensions and incubated for 2 or 16 h at 4, 25 and 37 °C at pH 7.2. A concentration-, temperature- and exposure time-dependent response was showed by GTE in suspension tests, where complete inactivation was achieved after overnight exposure at 37 °C for both viruses and also at 25 °C for HAV.In addition, antiviral effect of GTE proved efficient in the surface disinfection tests since 1.5 log reduction and complete inactivation were recorded for MNV and HAV on stainless steel and glass surfaces treated with 10 mg/ml GTE for 30 min, analyzed in accordance with ISO 13697:2001. GTE was also evaluated as a natural disinfectant of produce, showing 10 mg/ml GTE reduced MNV and HAV titers in lettuce and spinach by more than 1.5 log after 30 min treatment.The results show a potential of GTE as natural disinfectant able to limit enteric viral (cross-)contaminations conveyed by food and food-contact surfaces.

The Effect of Green Tea Extract on Fat Oxidation during 1hour Arm Cycle Exercise

PURPOSE: Decaffeinated Green Tea Extract (GTE) supplementation can increase fat oxidation during leg exercise, but many people cannot perform leg exercise. The purpose of this study was to investigate the effect of GTE supplementation on fat utilization during 1h arm cycle exercise. We hypothesized that GTE supplementation will increase lipolysis and fat oxidation. METHODS: This was a randomized, controlled, triple blind study with a crossover design. 8 healthy adults (4 females, 23-37yrs) performed an incremental arm cycle test to exhaustion followed by 4 time trials at fixed workloads. After an 8h fast subjects did 1h of arm cycling at 50% Wpeak. Subjects were randomly assigned to either decaffeinated GTE (650mg, 611mg EGCG) or placebo (PLA) for 4wks. Subjects then repeated the 1hr arm cycle trial. A 4wk washout period was followed by the corresponding crossover trial. 5ml of blood were drawn pre and post exercise while respiratory gases were collected continuously. Plasma glycerol and free fatty acid (FFA) concentrations were assessed with commercially available analysis kits. The study was powered at 1-β > 95%, with α = 0.05 given a reported effect size of F = 3.39 for the time by treatment interaction. RESULTS: Mean VO2 during all 1hr trials showed no significant differences (.83-.89 ± .19-.25 L/min, p = .460). Similarly, mean total energy expenditure (EE) showed no differences across all trials (264.58 - 266.15 kcal, p = .420). The percentage of total EE from fat oxidation was higher after GTE supplementation compared to PLA, but this difference was not significant (22.83 ± 11.57 to 25.38 ± 11.3 % vs. 23.39 ± 9.97 to 20.69 ± 8.9%, p = .532). There were no significant changes in g/min of fat oxidized between treatments before and after supplementation (GTE = .11 ± .08 to .12 ± .06 vs. PLA = .10 ± .05 to .09 ± .04, p = .220. Blood Glycerol concentration increased post exercise in all trails, with no significant differences between treatments (8.55 ± 3.44 mg/dl to 8.47 ± 2.6 vs. 10.05 ± 2.86 to 8.99 ± 3.51 mg/dl, p = .527). FFA concentration was also increased post exercise for both groups with no significant difference between treatments (8.30 ± 3.8 mg/dl to 8.69 ± 2.59 vs. 9.06 ± 4.49 to 7.16 ± 3.79, p = .234). CONCLUSION: These results suggest that there is no effect of 1m GTE supplementation on fat utilization during 1hr arm cycle exercise at 50% Wpeak.

The Effect of Green Tea Leaf Extract on Spatial Memory Function and Superoxyde Dismutase Enzyme Activity in Mice with D-galactose Induced Dimentia

Background:�Oxidative stress�and�inflammation�play�an important role in�pathogenesis of�brain aging�and neurodegenerative diseases such as�Alzheimer. Green tea�has been shown to have antioxidant, anti-inflammatory, anticancer, and neuroprotective�activity. Objectives: to determine the effect of green tea extract on�spatial memory function and superoxide dismutase�enzyme activity in mice with D-galactose induced dementiaMethods:�An experimental study using "post test only control group design".�Twenty male�BALB/c Mice aged 6-8�weeks were divided into�4�groups.�Negative control group�(NG)�was induced by subcutaneous injection of�D-galactose�(150�mg/kg BW)�once�daily for�6�weeks.�GT-90, GT-270,�GT-540�were induced by�D-galactose�and orally administered with 90, 270, and 540 mg/kg BW of�green tea extract�once daily for 6 weeks.�The spatial memory functions were assessed using Morris water maze and�SOD enzyme activities�were evaluated using ELISA.�One-way Anova and Kruskal-Wallis were used for statistical analysis.�Results: mean�percentage of latency time in the GT-90�(35.29�(SD=�2.69)%),�GT-270�(35.28 (SD= 2.62)%), and�GT-540�(35.62�(SD=5.05)%)�were�significantly�higher compared to that of NG�(20.38�(SD =�3.21)%), p�&lt;0.05). SOD enzyme activity in the�GT-270�(0.78 (SD = 0.07) U/ml)�was�significantly�higher�compared to that of NG�(0.51 (SD = 0.01) U�ml), p= 0.004).Conclusion:�Green tea extract�may�improve�spatial memory�function�and�the activity of�superoxide dismutase�enzyme in mice�with D-galactose induced�dementia.

Heterocyclic aromatic amines content in chicken burgers and chicken nuggets sold in fast food restaurants and effects of green tea extract and microwave thawing on their formation

The aims of the study were to investigate the presence of heterocyclic aromatic amines (HCAs) in chicken burgers (CBs) and chicken nuggets (CNs) purchased from fast food restaurants and the effects of green tea extract addition (GTE) into the covering material as well as microwave thawing (MT) on HCAs formation in CBs and CNs. Analysis of HCAs (IQx, IQ, MeIQx, MeIQ, 7,8-DiMeIQx, 4,8-DiMeIQx, PhIP, AαC, and MeAαC) were performed using HPLC-DAD following solid phase extraction. While the total HCAs content varied between not quantified—9.72 ng/g meat and 1.94–22.01 ng/g meat for commercial CBs and CNs, it ranged between not quantified—3.42 ng/g meat and not quantified—0.31 ng/g meat for CBs and CNs produced in the laboratory, respectively. Commercial CBs and CNs contained high amounts of HCAs, with MeIQx being the dominant HCA in all analyzed samples however, GTE and MT did not reduce the HCAs formation in CBs and CNs. Practical applications Widely consumed fast food chicken products; chicken burgers and chicken nuggets contain high amounts of HCAs. That much HCA concentration in chicken burgers and chicken nuggets can endanger the public health seriously.

UPLC-ESI-MS/MS study of the effect of green tea extract on the oral bioavailability of erlotinib and lapatinib in rats: Potential risk of pharmacokinetic interaction.

Green tea (GT) is one of the most consumed beverages worldwide. Tyrosine kinase inhibitors (TKIs) belong to the oral targeted therapy that gained much interest in oncology practice, among which are erlotinib (ERL) and lapatinib (LAP). Since green tea polyphenols (GTP) are known to be inhibitors of receptor tyrosine kinases, GTE could likely potentiate the anticancer effect of TKIs, but with a possibility of pharmacokinetic (PK) interaction with co-administered TKIs. In this study, the effect of GTE on the PK of ERL/LAP in rats was studied. UPLC-ESI-MS/MS method has been developed and validated for the quantification of ERL and LAP in rat plasma, using gefitinib (GEF) as the internal standard. Plasma samples were treated extensively by protein precipitation (PPT) followed by solid phase extraction (SPE) using octadecyl C 18/14% cartridges. Chromatographic analysis was carried out on Acquity UPLC BEH™ C18 column with a mobile phase consisting of water: acetonitrile (20: 80, v/v), each with 0.15% formic acid. Quantification was performed in the positive electrospray ionization (ESI+) mode with multiple reaction monitoring (MRM) of the transitions m/z 394.29→278.19 (ERL), m/z 581.07→365.13 (LAP), and m/z 447.08→128.21 (GEF). The method was fully validated as per the FDA guidelines showing linearity over the range of 0.4-1000 (ERL) and 0.6-1000 (LAP) ng/mL with very low lower limit of quantification (LLOQ) of 0.4 and 0.6ng/mL for ERL and LAP, respectively. The applicability of the method was extended to perform a comparative study of the PK of ERL/LAP following short-term and long-term administration of GTE, compared with their single oral administration. The results revealed that a significant reduction in the oral bioavailability was recorded with both ERL and LAP following the ingestion of GTE particularly for short-term administration. A reduction in Cmax (AUC) by 67.60% (69.50%) and 70.20% (73.96%), was recorded with short-term administration of GTE, compared with only 16.03% (21.09%) and 13.53% (22.12%) reduction for ERL and LAP, respectively, with long-term administration. Thus patients taking TKIs should preferably avoid drinking GT or ingesting GTE capsules during the period of treatment with TKIs.

Therapeutic effect of green tea extract on alcohol induced hepatic mitochondrial DNA damage in albino wistar rats

The present study principally sought to investigate the effect of green tea extract (GTE) supplementation on hepatic mitochondrial DNA (mtDNA) damage in alcohol receiving rats. MtDNA was isolated from hepatic tissues of albino wistar rats after alcohol treatment with and without GTE supplementation. Entire displacement loop (D-loop) of mtDNA was screened by PCR-Sanger’s sequencing method. In addition, mtDNA deletions and antioxidant activity were measured in hepatic tissue of all rats. Results showed increased frequency of D-loop mutations in alcoholic rats (ALC). DNA mfold analysis predicted higher free energy for 15507C and 16116C alleles compared to their corresponding wild alleles which represents less stable secondary structures with negative impact on overall mtDNA function. Interestingly, D-loop mutations observed in ALC rats were successfully restored on GTE supplementation. MtDNA deletions were observed in ALC rats, but intact native mtDNA was found in ALC+GTE group suggesting alcohol induced oxidative damage of mtDNA and ameliorative effect of GTE. Furthermore, markedly decreased activities of glutathione peroxidise, superoxide dismutase, catalase and glutathione content were identified in ALC rats; however, GTE supplementation significantly (P<0.05) restored these levels close to normal. In conclusion, green tea could be used as an effective nutraceutical against alcohol induced mitochondrial DNA damage.