Effect Of Different Concentrations Research Articles

Anti-Toxoplasma In Vitro and In Vivo Activity of Pyrus boissieriana Arbutin-Rich Fraction.

Pyrus boissieriana is a rich source of arbutin and has been used in herbal medicine to treat infectious diseases. This study aimed to investigate the effect of the arbutin-rich fraction of Pyrus boissieriana aerial parts on Toxoplasma gondii In Vitro and In Vivo. An arbutin-rich fraction of P. boissieriana was prepared beforehand. Flow cytometry was used to evaluate the effect of different concentrations (1-512µg/ml) of the P. boissieriana arbutin-rich fraction on Toxoplasma tachyzoites (RH strain). The cytotoxicity of the concentrations on the macrophage J774 cell line was also investigated by MTT assay. For In Vivo investigation, 4-6-week-old female mice infected with the RH strain of T. gondii were treated with different doses (16, 32, 64, 256, and 512mg/kg) of the fraction using gavage. The highest and lowest lethality of the tachyzoites were 89.6% and 25.9% related to the concentrations of 512µg/ml and 1µg/ml, respectively, with an IC50 value of 18.1µg/ml ± 0.37. The cytotoxicity test showed an IC50 value of 984.3µg/ml ± 0.76 after 48h incubation. The mean survival of mice at the lowest treated dose (16mg/kg) was 6.6days, and it was 15days at the highest dose (512mg/kg). The concentrations of 512, 256, 128, and 64mg/kg of the fraction compared to the negative control (6.2days mean survival) significantly increased the survival time of mice (P < 0.001, P = 0.009, P = 0.018, and P = 0.021, respectively). The results showed that the arbutin-rich fraction of P. boissieriana is effective against T. gondii In Vitro and In Vivo and may be a reliable alternative to conventional treatment for toxoplasmosis, although further studies are necessary.

Comparison of biosynthetic zinc oxide nanoparticle and glucantime cytotoxic effects on Leishmania major (MRHO/IR/75/ER).

Currently, zinc oxide (ZnO) particles are used in nanotechnology to destroy a wide range of microorganisms. Although pentavalent antimony compounds are used as antileishmanial drugs, they are associated with several limitations and side effects. Therefore, it is always desirable to try to find new and effective treatments. The aim of this research is to determine the antileishmanial effect of ZnO particles in comparison to the Antimoan Meglumine compound on promastigotes and amastigotes of Leishmania major (MRHO/IR/75/ER). After the extraction and purification of macrophages from the peritoneal cavity of C57BL/6 mice, L. major parasites were cultured in Roswell Park Memorial Institute-1640 culture medium containing fetal bovine serum (FBS) 10% and antibiotic. In this experimental study, the effect of different concentrations of nanoparticles was investigated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric method, in comparison to the glucantime on promastigotes, amastigotes and healthy macrophages in the culture medium. The amount of light absorption of the obtained color from the regeneration of tetrazolium salt to the product color of formazan by the parasite was measured by an enzyme-linked immunosorbent assay (ELISA) reader, and the IC50 value was calculated. IC50 after 24 h of incubation was calculated as IC50 = 358.6 µg/mL. The results showed, that the efficacy of ZnO nanoparticles was favorable and dose-dependent. The concentration of 500 µg/mL of ZnO nanoparticles induced 84.67% apoptosis after 72. Also, the toxicity of nanoparticles was less than the drug. Nanoparticles exert their cytotoxic effects by inducing apoptosis. They can be suitable candidates in the pharmaceutical industry in the future.

A potential trade-off between offense and defense in honeybee innate immunity: Reduced phagocytosis in honeybee hemocytes correlates with a protective response after exposure to imidacloprid and amitraz

Phagocytosis “offense” is a crucial process to protect the organism from diseases and the effects of foreign particles. Insects rely on the innate immune system and thus any hindrance to phagocytosis may greatly affect their resistance to diseases and response to pathogens. The European honeybee, a valuable species due to its economic and environmental contribution, is being challenged by colony collapse disorder leading to its decline. Exposure to multiple factors including pesticides like imidacloprid and amitraz may negatively alter their immune response and ultimately make them more susceptible to diseases. In this study, we compare the effect of different concentrations and mixtures of imidacloprid and amitraz with different concentrations of the immune stimulant, zymosan A. Results show that imidacloprid and amitraz have a synergistic negative effect on phagocytosis. The lowered phagocytosis induces significantly higher hemocyte viability suggesting a negatively correlated protective mechanism “defense” from pesticide-associated damage but may not be protective from pathogens.

ANTI DERMATOPHYTIC EFFECT OF KUSHTAGHNA DASHEMANI IN DADRU KUSHTA (DERMATOPHYTOSIS), AN IN-VITRO STUDY

Background: Shadvirechanashatashritiya adhyaya of Charaka Samhita describes fifty group of drugs (Panchashanmahakashaya) and Kushtaghna dashemani is one among them. Dadru is classified under Kshudra kushta by Acharya Charaka. On the basis of presenting symptomatology Dadru kushta can be interpreted as Dermatophytosis. The prevalence of superficial fungal infection in India is 27.6% of which Dermatophytes are the most common agents (75.6%) though the disease is not a life threatening, the relapsing nature of this disease makes it much annoyance for patient and bothersome for physician too. Hence an attempt was made to evaluate the effect of Kushtaghna Dashemani on Dadru Kushta through in-vitro study. The In-vitro study on Anti- dermatophytic action of Kushtaghna dashemani Kashaya was carried out by Agar Well diffusion method on the dermatophyte species Trichophyton tonsurans, Microsporum Canis and Trichophyton rubrum with different concentrations of Kushtaghna dashemani Kashaya. Anti-dermatophyte effect of different concentrations of Kushtaghna dashemani kashaya was seen at the different volume used against the taken dermatophytes species.

Experimental study on environmental chemical effect and purification treatment of waste slurry in horizontal directional drilling

The waste slurry generated during Horizontal Directional Drilling (HDD) has detrimental effects on water sources, soil, and organisms. Existing studies lack quantitative analysis and assessment of the environmental hazards of waste slurry. The treatment of waste slurry is usually done by physical curing or chemical curing agents. The treatment effect of these treatments is unsatisfactory or not environmentally friendly enough. Research on the application of Microbially Induced Calcite Precipitation (MICP) in waste slurry purification can help provide innovative solutions. This study began by acquiring the basic properties, mineral composition, and particle size distribution of the waste slurry through experiments. The effects of different concentrations of waste slurry on soil chemical properties were compared, including the measurement of total dissolved solids (TDS), turbidity, and heavy metal ion concentrations of waste slurry supernatant. Additionally, the environmental impact of the waste slurry was assessed from an electrochemical, turbidity, and ionic composition perspectively. A combination formulation containing 2.8 wt% urease, 5 wt% urea, and 3.5 wt% calcium chloride was used to purify the waste slurry. The supernatant of the MICP treated waste slurry undergoes electrochemical testing to evaluate the effectiveness of MICP technology for slurry treatment. The findings indicate that both chemical additives and stratum contaminants significantly contribute to slurry pollution. When the waste slurry constitutes more than 40 wt% of the soil mass fraction, the electrical conductivity (EC) of the soil surpasses the threshold of 1000 μs/cm, and the pH level rises to approximately 9.5, rendering it unsuitable for plant growth. Moreover, the TDS, turbidity, and heavy metal ion concentrations in the waste slurry supernatant exceed the permissible environmental discharge limits. Notably, the concentration of arsenic (As) exceeds the national standard by 18 times, cadmium (Cd) by 20 times, and selenium (Se) by 208 times, posing a high risk of environmental pollution. However, the comparison of ion concentrations in the supernatant before and after MICP treatment reveals that MICP demonstrates effective cementation and co-precipitation of heavy metal ions. MICP purified 66.6 % of arsenic (As), all cadmium (Cd), 75 % of phosphorus (P), and 76 % of selenium (Se) from the waste slurry supernatant. The results of this research contribute to the quantitative evaluation of the environmental hazards of waste slurry. It also helps to promote the application of MICP in waste slurry purification.

Effects of different concentrations and particle sizes of microplastics on the full life history of freshwater Chlorella

Microplastics (MPs) as pollutants can have adverse effects on aquatic ecosystems; however, their effects on the full life history of microalgae need to be further explored and thoroughly examined. In this study, we investigated influence of polystyrene (PS) plastics with different concentrations (10/50/100 mg/L) and particle sizes (0.1/0.5/1 μm) on the full life history of Chlorella; their potential environmental risks were also analyzed. The results showed that PS(0.1um) had the strongest inhibitory effect on Chlorella growth (Max(inhibition) 68.42%), PS(0.5/1um) can not only promote (Max(promotion) 55.48% and 55.05%) but also prolong cell growth; PS has various effects on photosynthetic efficiency of Chlorella. PS(0.1um) can significantly promote Fv/Fm, inhibit RC/ABS, F0/Fv, DIo/RC, and both inhibit and promote rETRmax, but effect of PS(0.5/1μm) is generally consistent with that of control group; PS affects the morphological structure and interaction of Chlorella significantly, and can squeeze and aggregate cells. Zeta potential fluctuated greatly in the initial stage of experiment, and was stable as Relative conductivity in the later stage. About 65.5% of PS(0.1um) can enter cell, which has potential risk of entering the food chain; Statistics on long and short-term impacts showed significant differences in growth and photosynthesis efficiencies, as well as in interactions; the potential environmental risk index (PERI) indicates that class II (slightly polluted) has the highest percentage (64.72%), and that the concentration and composition of MPs are important influences on potential environmental risk. Overall, the long-term impacts of PS were diverse, but Chlorella also showed good resilience. Meanwhile, we found that most of the previous short-term studies may be one-sided and incomplete, the real impacts of MPs may be overestimated. Our research could provide scientific support for assessing the risks posed by MPs.

Sodium lauryl sulphate-mediated synthesis: Unravelling the optimization path for enhanced stability and optical properties of cadmium sulphide nanoparticles

This research paper explores the synthesis of cadmium sulphide nanoparticles using sodium lauryl sulphate as a stabilizing agent through the co-precipitation method. The structural properties were investigated using X-ray diffractometry, while optical properties were analysed through UV–visible absorption spectroscopy. High-resolution transmission electron microscopy and energy-dispersive X-ray spectroscopy were employed to study the morphology and elemental mapping of the nanoparticles. The co-precipitation synthesis, synergized with sodium lauryl sulphate assisted stabilization, affords a scientific vantage point into the intricate mechanisms through which sodium lauryl sulphate intricately tailors the optical and stability profiles of nanoparticles. Additionally, the effects of different concentrations of sodium lauryl sulphate were examined to determine the optimal concentration for desired properties. The optimal concentration of 1.5 g yielded cadmium sulphide nanoparticles with desirable characteristics, featuring a band gap of 2.07 eV. The findings presented herein contribute significantly to advancing our understanding of sodium lauryl sulphate capped cadmium sulphide nanoparticles and highlight their potential application in enhancing photocatalyst efficiency.This research holds exciting prospects for the creation of eco-friendly and highly efficient innovations, with extensive implications for the environmental and industrial sectors.

Effects of Cu (II) on the Growth of Chlorella vulgaris and Its Removal Efficiency of Pollutants in Synthetic Piggery Digestate.

C. vulgaris has a positive effect on the removal of nutrients from pig farm biogas slurry. However, swine wastewater often contains heavy metal ions, such as Cu (II), which may have impacts on the nutrient removal performance of C. vulgaris. Additionally, the heavy metal ions in wastewater can be adsorbed by microalgae. In this study, the stress effect of Cu (II) on the growth of Chlorella vulgaris, the Cu (II) removal by microalgae, and the effect of different concentrations of Cu (II) on the nutrient removal efficiency of C. vulgaris in biogas slurries were explored. The results showed that the microalgae biomass of microalgae on the sixth day of the experiment was the highest in the treatment with a Cu (II) concentration of 0.5 mg/L, which was 30.1% higher than that of the 2.5 mg/L group. C. vulgaris had higher removal efficiencies of Cu (II) at a Cu (II) concentration of 0.1~1.5 mg/L. The-OH, C=O, -COOH, and C-O groups on the surface of the algal cells play a significant role in the removal of Cu (II). The removal rates of COD, NH3-N, TN, and TP by C. vulgaris at a Cu (II) concentration of 0.5 mg/L were the highest, which were 89.0%, 53.7%, 69.6%, and 47.3%, respectively.

Effect of different concentrations of cocoa (Theobroma cacao L.) and coffee (Coffea arabica) mucilage in the preparation of an energy drink

El mucílago de cacao es un subproducto muy poco industrializado debido al desconocimiento de sus propiedades fisicoquímicas y a la carencia de innovación tecnológica para su manejo y transformación. Es por ello, que la presente investigación tuvo como objetivo evaluar el efecto de distintas concentraciones de mucílago de cacao y café en la elaboración de una bebida energética. Se utilizó un Diseño Completamente al Azar (D.C.A), evaluando 6 tratamientos con tres repeticiones, para determinar diferencias estadísticas se aplicó una prueba de significación Tukey (p&lt;0.05). Las distintas concentraciones de mucílago de cacao y café influyeron significativamente en las características fisicoquímicas (pH, acidez titulable, °Brix, ceniza, humedad, proteína, densidad y carbohidratos) de la bebida, también, los tratamientos presentaron un alto contenido de energía (43,08kcal a 58,60kcal). En relación a las características sensoriales, el T5 = 20% pulpa de mucílago de cacao nacional y 1% de café, obtuvo una mayor aceptación por parte de los catadores con la categoría “me gusta mucho”, además, presentó un color ámbar, sabor y aroma con leves notas a cacao-café. Con esto, se concluye que el mucílago de cacao, puede ser una alternativa en el mercado de las bebidas energéticas.

Mechanism of Hirudin-Mediated Inhibition of Proliferation in Ovarian Cancer Cells.

To investigate the inhibitory effect of hirudin on the cell proliferation of human ovarian cancer A2780 cells by preventing thrombin and its underlying molecular mechanism. Cell Counting Kit-8 (CCK-8) method was used to detect the effect of different concentrations of hirudin and thrombin on the cell proliferation of A2780 cells. PAR-1 wild-type overexpression plasmid was constructed utilizing enzyme digestion identification, and it was transferred to A2780 cells. Sequencing and Western blot were used to detect the changes in PAR-1 protein expression. Western blot detection of PKCα protein phosphorylation in A2780 cells was performed. We also implemented quantitative PCR to detect the mRNA expression levels of epithelial-mesenchymal transition (EMT)-related genes, CDH2, Snail, and Vimentin, in A2780 cells. 1μg/ml hirudin treatment maximally inhibited the promotion of A2780 cell proliferation by thrombin. Hirudin inhibited the binding of thrombin to the N-terminus of PAR-1, hindered PKCα protein phosphorylation in A2780 cells, and downregulated the mRNA expression levels of CDH2, Snail, and Vimentin. In conclusion, hirudin inhibits the cell proliferation of ovarian cancer A2780 cells, and the underlying mechanism may be through downregulating the transcription level of EMT genes, CDH2, Snail, and Vimentin. This study indicates that hirudin may have a therapeutic potential as an anti-cancer agent for ovarian cancer.

Evaluation of Peracetic Acid Treatment on Beef Trimmings and Subprimals Against Salmonella and E. Coli O157:H7 Within Regulatory Retained Water Limitations

The application of antimicrobial treatments to beef trimmings prior to grinding for the reduction of microbial contamination in ground beef has increased recently. However, raw single-ingredient meat products are not permitted by Food Safety and Inspection Services (FSIS) to retain more than 0.49% water resulting from postevisceration processing. The effectiveness of antimicrobials with the limited water retention is not well documented. The objective of this study was to determine the effectiveness of peracetic acid at varied concentrations against E. coli O157:H7 and Salmonella on the surface of beef trimmings and beef subprimals that was applied at industry operating parameters within the retained water requirement. One hundred and forty-four each of beef trimmings and subprimals were used to evaluate the effect of different concentrations of peracetic acid solution on reducing E. coli O157:H7 and Salmonella on surfaces of fresh beef within the FSIS requirement of ≤0.49% retained water from antimicrobial spray treatments using a conveyor system. A ten-strain cocktail mixture was inoculated on surfaces of fresh beef and subjected to water or four different concentrations of peracetic acid (130, 150, 200, and 400 ppm). Spray treatments with 130, 150, and 200 ppm peracetic acid reduced (P ≤ 0.05) E. coli O157:H7 and Salmonella at least 0.2 log on surfaces of beef trimmings and subprimals. Spray treatment with 400 ppm peracetic acid resulted in approximately 0.5 and 0.3 log reduction of E. coli O157:H7 and Salmonella, respectively. Results indicate that all concentrations (130–400 ppm) of peracetic acid significantly reduced E. coli O157:H7 and Salmonella on beef trimmings and subprimals compared to untreated controls. Thus, a range from 130 to 400 ppm of peracetic acid can be used during beef processing to improve the safety of beef trimmings and subprimals when weight gain is limited to ≤0.49% to meet regulatory requirements.

High-sensitivity flip chip blue Mini-LEDs miniaturized optical instrument for non-invasive glucose detection

The colorimetric detection of glucose typically involves a peroxidase reaction producing a color, which is then recorded and analyzed. However, enzyme detection has difficulties with purification and storage. In addition, replacing enzyme detection with chemical methods involves time-consuming steps such as centrifugation and purification and the optical instruments used for colorimetric detection are often bulky and not portable. In this study, ammonium metavanadate and sulfuric acid were used to prepare the detection solution instead of peroxidase to produce color. We also analyzed the effect of different concentrations of detection solution on absorbance sensitivity. Finally, a flip chip blue Mini-LEDs miniaturized optical instrument (FC blue Mini-LEDs MOI) was designed for glucose detection using optics fiber, collimating lenses, a miniaturized spectrometer, and an FC Blue Mini-LEDs with a center wavelength of 459 nm. While detecting glucose solutions in the concentration range of 0.1–10 mM by the developed MOI, the regression equation of y = 0.0941x + 0.1341, R2 of 0.9744, the limit of detection was 2.15 mM, and the limit of quantification was 7.163 mM. Furthermore, the preparation of the detection solution only takes 10 min, and the absorbance sensitivity of the optimized detection solution could be increased by 2.3 times. The detection solution remained stable with only a 0.6% decrease in absorbance compared to the original after storing it in a refrigerated environment at 3 °C for 14 days. The method proposed in this study for detecting glucose using FC blue light Mini-LEDs MOI reduces the use of peroxidase. In addition, it has a wide detection range that includes blood as well as non-invasive saliva and tear fluids, providing patients with a miniaturized, highly sensitive, and quantifiable glucose detection system.

The effect of different concentrations of laminarin on the quality of cryopreserved ram semen

BACKGROUND: Increasingly, sheep breeders are using artificial insemination to produce lambs, so finding methods that preserve ram sperm can be useful. OBJECTIVE: To determine the protective effects of different concentrations of laminarin on ram sperm motility, viability, abnormalities, membrane, and DNA integrity, superoxide dismutase enzyme (SOD) activity, and malondialdehyde (MDA) production after freeze-thawing. MATERIALS AND METHODS: The ejaculates of four rams were collected and stored at 35 °C. Semen samples were diluted with a tris-base extender containing 100, 200, 400, and 800 μg/mL of laminarin and a control extender containing no laminarin, then frozen in liquid nitrogen after 4 h in the refrigerator. RESULTS: In the treatment of frozen-thawed spermatozoa with 800 μg/mL laminarin, motility, viability, membrane integrity, and DNA integrity were significantly higher than in the control. In spermatozoa that were exposed to 800 μg/mL laminarin after thawing, MDA production was significantly lower than in the control group. The percentage of abnormal spermatozoa in 800 μg/mL laminarin was significantly lower than that in the control. CONCLUSION: The addition of 800 μg/mL laminarin to the freezing extender increases motility, viability, SOD activity, and plasma membrane integrity, while reducing abnormality and MDA production in freeze-thawed ram semen.

Evaluation of antioxidant properties and cytotoxicity of brown algae (nizamuddinia zanardinii) in uterine (hela) and pancreatic cancer cell lines (paca-2).

Pancreatic cancer and cervical cancer are among the most common cancers. Brown algae have anti-inflammatory, anti-cancer, anti-fungal, antioxidant, and immune-boosting properties. This study investigated the antioxidant properties and the effect of brown algae extract on pancreatic and uterine cancer cells. In this study, Cervical (Hela) and pancreas (Paca-2) cancer cell lines were examined. The algae materials were extracted by sequential maceration method and amount of fucoxanthin content in the sample was determined by using High Performance Liquid Chromatography (HPLC) system. The cytotoxic effect of different concentrations of brown algae was measured by the MTT assay. All statistical calculations for comparing IC50 were analyzed using Graph Pad Prism software. the algal sample contained an average of 102.52 ± 0.12μg of fucoxanthin per 100g. IC50 for 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide free radical scavenging activity for methanolic extract was 2.02 and 11.98 ± 0.13 respectively. Brown algae in all fractions inhibited cell growth and survival. In Hela cell lines, the methanolic extract was the most effective inhibitor, while in Paca cell lines, hexane and methanolic extracts were particularly potent. The methanolic extract was more toxic than other fractions on Hela and Paca cell lines. This study highlights brown algae extracts strong anticancer effects on uterine and pancreatic cancer cells, suggesting its potential as a natural anticancer drug. Different fractions of the extract showed superior apoptotic and cytotoxic effects, with higher concentrations leading to increased apoptotic effects and reduced survival rates of cancer cells.

Participation of glutamic acid in the catecholamines release from the amygdala of rabbits

Glutamic acid (Glu), as the most stimulating neurotransmitter, occurs in almost all structures of the central nervous system (CNS). Especially high concentrations are found in the structures of the motivational system (hypothalamus, hippocampus, amygdala, prefrontal cortex – mPFC) and the motor system (striatum, caudate nucleus, pallidum). Glu is also found in the autonomic nervous system (ANS), as well as in peripheral tissues and organs (in the adrenal glands, especially important in stress; the hypothalamic-pituitary-adrenal axis – HPA axis). Catecholamines (CA), including dopamine (DA), norepinephrine (NE), and/or epinephrine (E), are major neurotransmitters that mediate various CNS functions, such as motor control, cognition, emotion, memory processing, pain, stress, and endocrine modulation. The aim of the study was to investigate the in vitro effect of different concentrations of Glu (5, 50, and 200 μM in a volume of 1 ml of Krebs medium) on CA release from rabbit amygdala sections collected after decapitation of 12-week-old female rabbits. The same piece of tissue was transferred every 30 minutes to successive incubation wells containing Glu in an appropriate dose. The medium collected from the wells after 30, 60, and 90 minutes of incubation was frozen until DA, NE, and E analyses were performed by the RIA method. The results showed that Glu differently affected CA release from the amygdala. Generally, an inhibitory effect of Glu on CA release from the amygdala was observed. It was surprising to find the highest concentration of E in the amygdala compared to NA and even DA.

A simple fluorescence pyrocatechol-polyethyleneimine detection method for 3-MCPD.

A rapid fluorescence detection method was established for 3-monochloropropane-1,2-diol (3-MCPD). The detection system works based on the fluorescence quenching of pyrocatechol-polyethyleneimine (PCh-PEI) polymer by 3-MCPD. The fluorescence quenching ability of 3-MCPD for PCh-PEI polymer was measured at different pH and temperatures. Indeed, in the presence of 3-MCPD, the fluorescence intensity of PCh-PEI polymer solution was quenched best at 100 °C and pH 8.5. Also, the effect of different concentrations of 3-MCPD on the optical properties of the PCh-PEI polymer was examined. Under optimal experimental conditions, fluorescence detection was linear in a range of 0.08-2.0 mg per L 3-MCPD, with a calculated detection limit of 0.06 mg L-1 and a correlation coefficient of 0.9974. Concisely, the reported method has good sensitivity and can be used for the rapid detection of 3-MCPD contamination in food products.

Nitidine Chloride Inhibits Proliferation and Induces Mitochondrial-Mediated Apoptosis of Human Melanoma A375 and A2058 Cells In Vitro

Melanoma is a malignant tumor transforming from normal melanocytes, with strong invasion and low survival rate. Nitidine chloride (NC) is a natural benzophenidine alkaloid extracted from the roots of traditional Chinese medicine zanthoxylum nitidum. In recent years, many studies have found that NC may have strong anti-tumor activity. However, the efficacy of NC against melanoma has rarely been reported, and the potential molecular mechanism remains unknown. Our study explored the effects of NC on the proliferation and apoptosis of human melanoma A375 and A2058 cells. CCK-8 was utilized to detect the effects of different concentrations of NC on the proliferation of A375 and A2058 cells. Within a certain concentration and time range, NC can inhibit the viability of A375 and A2058 cells significantly in a time-dose-dependent manner. In addition, NC-induced apoptosis was confirmed by DAPI staining and flow cytometry analysis of Annexin V-FITC/PI. Moreover, we observed that the ratio of Bax/Bcl-2 in the cytoplasm increases, activating Caspase-3/-9 and ultimately inducing apoptosis via the mitochondrial pathway. Our study indicated that NC has anti-tumor properties against human melanoma cells through inhibiting proliferation and inducing apoptosis. Therefore, the results provide new insights for future work on the utilization of NC in malignant melanoma treatment.

Assessing the Efficacy of Metarhizium anisopliae (Metschnikoff) Sorokin as a Biological Control Agent against Rose Sawfly Larvae

Increasing concerns over the environmental impact of chemical pesticides have prompted the exploration of alternative, and eco-friendly solutions for controlling insect pests. The use of entomopathogenic fungus (EPF) as a biological control agent is of paramount importance. We aimed to investigate the effect of different concentrations of Metarhizium anisopliae (Metschnikoff) Sorokin on larval mortality and pupal formation, of sawfly larvae, a major pest of rose plants. Our study revealed a concentration-dependent effect of M. anisopliae on sawfly larvae. At 7 days after EPF exposure, the highest concentration (1 × 109 conidia/ml) led to a mortality rate of 65.0% and at the 11th day, the larval mortality was reached up to 82.5%. The highest EPF concentration resulted in a minimal pupal formation (7.5%). This study demonstrates the potential of M. anisopliae as a valuable biological control agent against sawfly larvae infestations in rose plants.

Effects of Different Concentrations of Sulfate Ions on Carbonate Crude Oil Desorption: Experimental Analysis and Molecular Simulation

Effects of Different Concentrations of Sulfate Ions on Carbonate Crude Oil Desorption: Experimental Analysis and Molecular Simulation

The Effect of Different Concentrations of Molybdenum Trioxide in Electrolyte for Dye-Sensitized Solar Cells (DSSCs): Performance Enhancement of DSSCs

The Effect of Different Concentrations of Molybdenum Trioxide in Electrolyte for Dye-Sensitized Solar Cells (DSSCs): Performance Enhancement of DSSCs