Effects of different concentrations and particle sizes of microplastics on the full life history of freshwater Chlorella

Abstract

Microplastics (MPs) as pollutants can have adverse effects on aquatic ecosystems; however, their effects on the full life history of microalgae need to be further explored and thoroughly examined. In this study, we investigated influence of polystyrene (PS) plastics with different concentrations (10/50/100 mg/L) and particle sizes (0.1/0.5/1 μm) on the full life history of Chlorella; their potential environmental risks were also analyzed. The results showed that PS(0.1um) had the strongest inhibitory effect on Chlorella growth (Max(inhibition) 68.42%), PS(0.5/1um) can not only promote (Max(promotion) 55.48% and 55.05%) but also prolong cell growth; PS has various effects on photosynthetic efficiency of Chlorella. PS(0.1um) can significantly promote Fv/Fm, inhibit RC/ABS, F0/Fv, DIo/RC, and both inhibit and promote rETRmax, but effect of PS(0.5/1μm) is generally consistent with that of control group; PS affects the morphological structure and interaction of Chlorella significantly, and can squeeze and aggregate cells. Zeta potential fluctuated greatly in the initial stage of experiment, and was stable as Relative conductivity in the later stage. About 65.5% of PS(0.1um) can enter cell, which has potential risk of entering the food chain; Statistics on long and short-term impacts showed significant differences in growth and photosynthesis efficiencies, as well as in interactions; the potential environmental risk index (PERI) indicates that class II (slightly polluted) has the highest percentage (64.72%), and that the concentration and composition of MPs are important influences on potential environmental risk. Overall, the long-term impacts of PS were diverse, but Chlorella also showed good resilience. Meanwhile, we found that most of the previous short-term studies may be one-sided and incomplete, the real impacts of MPs may be overestimated. Our research could provide scientific support for assessing the risks posed by MPs.