Effect Of Different Concentrations Research Articles

Investigation of copper-induced intestinal damage and proteome alterations in Takifugu rubripes: Potential health risks and environmental toxicology detection

Copper is one of the predominant water pollutants. Excessive exposure to copper can cause harm to animal health, affecting the central nervous system and causing blood abnormalities. Cuproptosis is a novel form of cell death that differs from previous programmed cell death methods. However, the impact of copper on the intestines remains unclear. Therefore, we investigated the effects of different concentrations of copper exposure on the intestinal proteome of Takifugu rubripes (T. rubripes). Relevant biomarkers were used to detect cuproptosis. We revealed the crosstalk relationship between cuproptosis and self-rescue at different concentrations, and discussed the feasibility of using potential cuproptosis indicators as anti-infection factors. We observed intestinal damage in the three copper exposure groups, especially in T. rubripes treated with 100 and 500 μg/L copper, with shedding and breakage of intestinal villus and fuzzy and loose structure of intestinal mucosa. The presence of copper stress not only causes cuproptosis but also oxidative damage caused by reactive oxygen species (ROS). The results of quantitative proteomics by TMT showed that compared to the 50 and 100 μg/L copper exposure groups, the expression of glutaminase, pyruvate kinase, and skin mucus lectin in the 500 μg/L group was significantly increased. The positive mediators COX5A and CTNNB1, as well as the negative mediators CD4 and FDXR, were found to be differentially expressed. Using the protein expression trends of cuproptosis indicator factors FDX1 and DLAT to indicate the concentration of copper ions in the environment. In addition, we found a new effect of promoting ferroptosis: providing additional copper ions can activate the phenomenon of ferroptosis. Our results expand our understanding of the potential health risks of copper in T. rubripes. At the same time, it is of great significance for the process of copper poisoning and the development of new environmental toxicology detection reagents.

Optimized medium composition in Physalis alkekengi callus culture altered nitric oxide level for inducing antioxidant enzyme activities and secondary metabolites

Physalis alkekengi L. is a valuable medicinal plant from the Solanaceae family and has multiple therapeutic applications. This study aimed to develop an optimized protocol for callogenesis in P. alkekengi to obtain friable calluses with high biomass. The effect of different concentrations of picloram, casein hydrolysate (CH), basal media (Murashige and Skoog (MS) and Gamborg (B5)), and static magnetic field (SMF) were investigated on the callus induction and growth, signaling molecules, and enzymatic and non-enzymatic antioxidants. Results showed that CH (200 mgL−1) and SMF4 mT for 90 min increased callus induction and fresh weight in P. alkekengi, while different concentrations of picloram reduced callogenesis. Hypocotyl explants showed various callogenesis and metabolic responses depending on the basal medium type. The 2B5 medium supplied with CH 200 (mgL−1) induced friable and cream calluses with high biomass (0.62 g) compared to the MS medium (control). The maximum activity of superoxide dismutase and catalase activities was identified in the 2B5 medium and peroxidase in the 2MS medium. The highest total phenolic (129.44 µg g−1DW) content and phenylalanine-ammonia lyase activity were obtained in the 2MS medium, and total withanolides (49.86 µg g-1DW) and DPPH radical scavenging activity were observed in the 2B5 medium. The 2MS medium boosted the hydrogen peroxide and nitric oxide levels, while their contents alleviated in the 2B5 medium, although these parameters were higher than the control. The findings of this study suggest that an effective protocol for successful callogenesis in P. alkekengi and the nutrient composition of culture medium by affecting the level of signaling molecules can control the antioxidant defense system and callus growth.

Effects of Different Concentrations of Weak Acid Fracturing Fluid on the Microstructure of Coal

Effects of Different Concentrations of Weak Acid Fracturing Fluid on the Microstructure of Coal

Negative effects of cigarette butt leachate on freshwater phytoplankton communities.

We experimentally tested the effects of different concentrations of cigarette butt leachate on freshwater phytoplankton chlorophyll-a, species richness, cell density, and community composition. For this, we sampled the phytoplankton from a eutrophic lake and acclimated it for 24 h in microcosms. We then conducted the experiment in microcosms maintained for 96 h. The experiment consisted of four treatments: control and leachate from 1 butt L-1 (T1), 5 butts L-1 (T5), and 10 butts L-1 (T10), which were prepared by diluting a stock solution of leachate from 50 butts L-1. We found that algal chlorophyll-a content was not affected by different leachate concentrations. In contrast, phytoplankton cell density decreased in a dose-dependent manner as concentrations of the leachate increased. Similarly, the number of species was highest in the control group relative to all other treatments, with T1 and T5 showing higher species richness than T10. Additionally, the exposition to different concentrations of the leachate impacted community composition across all treatments in comparison to the control group. Our results suggest that cigarette butt leachate alters the number of cells and species, as well as the distribution of abundance, without necessarily reducing chlorophyll-a concentrations. Our findings indicate that to gain a comprehensive understanding of the effects of cigarette butt leachate on freshwater ecosystems, it is essential to evaluate more realistic scenarios that incorporate aquatic communities, rather than isolated species.

Focused review on factors affecting martensitic stainless steels and super martensitic stainless steel passive film in the oil and gas field

AbstractMartensitic and super martensitic stainless steels are widely used in the oil and gas industry for general corrosion mitigation in the presence of sweet corrosion (CO2) and sour corrosion (H2S), providing a cost-effective alternative to more expensive exotic corrosion-resistant alloys. Martensitic stainless steel is an approved material for construction when selecting tubular CO2 injection wells. This work aims to review the published literature on the subject of the operation limits of martensitic stainless steel and super martensitic stainless steel in high temperatures and high pressure under corrosive environments. Stress corrosion cracking (SCC) and sulfide stress corrosion cracking (SSCC) mechanisms on martensitic and super martensitic stainless steel surfaces are thoroughly analyzed. In this review paper, we have analyzed the factors that play a crucial role in passive film growth and passivity breakdown. The present work is to review the state of the art of mechanism responsible for SCC and SSCC susceptibility in different modified martensitic stainless steel materials, which are applied to the industry and lab scale. We have reviewed the effect of different concentrations of molybdenum content on SCC and SSCC susceptibility of conventional martensitic stainless steel, modified martensitic stainless steel, and super martensitic stainless steel. The effect of tempering temperature on the SCC and SSCC performance of the martensitic and super martensitic stainless steel was also studied. We also studied the effect of different concentrations of chromium on the improved corrosion-resistant properties and stability of passivation film.

Focused review on factors affecting martensitic stainless steels and super martensitic stainless steel passive film in the oil and gas field

AbstractMartensitic and super martensitic stainless steels are widely used in the oil and gas industry for general corrosion mitigation in the presence of sweet corrosion (CO2) and sour corrosion (H2S), providing a cost-effective alternative to more expensive exotic corrosion-resistant alloys. Martensitic stainless steel is an approved material for construction when selecting tubular CO2 injection wells. This work aims to review the published literature on the subject of the operation limits of martensitic stainless steel and super martensitic stainless steel in high temperatures and high pressure under corrosive environments. Stress corrosion cracking (SCC) and sulfide stress corrosion cracking (SSCC) mechanisms on martensitic and super martensitic stainless steel surfaces are thoroughly analyzed. In this review paper, we have analyzed the factors that play a crucial role in passive film growth and passivity breakdown. The present work is to review the state of the art of mechanism responsible for SCC and SSCC susceptibility in different modified martensitic stainless steel materials, which are applied to the industry and lab scale. We have reviewed the effect of different concentrations of molybdenum content on SCC and SSCC susceptibility of conventional martensitic stainless steel, modified martensitic stainless steel, and super martensitic stainless steel. The effect of tempering temperature on the SCC and SSCC performance of the martensitic and super martensitic stainless steel was also studied. We also studied the effect of different concentrations of chromium on the improved corrosion-resistant properties and stability of passivation film.

Immobilizing Antibody Biorecognition Layers on Au-TiO2 Thin Films: Direct (physisorption) vs. DSP-crosslinking (chemisorption) Surface Functionalization

Abstract The immobilization design strategy plays an important role in biosensor development and its sensing performance. Both adsorption (physisorption) and cross-linker functionalization (chemisorption) are common approaches for immobilizing a bioreceptor layer. In this work, these two approaches were studied and compared, envisaging a functional and strongly attached bioreceptor layer onto sputtered Au-TiO2 thin films. DSP cross-linker (Lomant's reagent) was used in the thin film’s functionalization, and the effect of different concentrations on the development of an adhesion layer was investigated. Surface morphology analysis of functionalized thin films suggested the development of uniformly coated self-assembled layers. However, DSP islands with a fractal structure were found for a concentration of 4 mg mL-1. Infrared spectroscopy confirmed the cross-linker functionalization at the thin film’s surface. Confocal microscopy of immobilized fluorescent antibodies revealed that DSP islands improve the chemical surface area available for bioreceptor immobilization. Moreover, an immunoassay using mouse IgG interaction with fluorescent anti-mouse IgG (Fab specific), working as capture and detection antibody, respectively, showed that DSP functionalization favors antibodies orientation and adhesion strength to the surface, when compared to physisorption.

Immobilizing Antibody Biorecognition Layers on Au-TiO2 Thin Films: Direct (physisorption) vs. DSP-crosslinking (chemisorption) Surface Functionalization

Abstract The immobilization design strategy plays an important role in biosensor development and its sensing performance. Both adsorption (physisorption) and cross-linker functionalization (chemisorption) are common approaches for immobilizing a bioreceptor layer. In this work, these two approaches were studied and compared, envisaging a functional and strongly attached bioreceptor layer onto sputtered Au-TiO2 thin films. DSP cross-linker (Lomant's reagent) was used in the thin film’s functionalization, and the effect of different concentrations on the development of an adhesion layer was investigated. Surface morphology analysis of functionalized thin films suggested the development of uniformly coated self-assembled layers. However, DSP islands with a fractal structure were found for a concentration of 4 mg mL-1. Infrared spectroscopy confirmed the cross-linker functionalization at the thin film’s surface. Confocal microscopy of immobilized fluorescent antibodies revealed that DSP islands improve the chemical surface area available for bioreceptor immobilization. Moreover, an immunoassay using mouse IgG interaction with fluorescent anti-mouse IgG (Fab specific), working as capture and detection antibody, respectively, showed that DSP functionalization favors antibodies orientation and adhesion strength to the surface, when compared to physisorption.

Development of Hybrid Electrospun Nanofibers: Improving Effects of Cellulose Nanofibers (CNFs) on Electrospinnability of Gelatin.

Cellulose nanofibers (CNFs) were used to improve the electrospinnability of the gelatin protein in a water/ethanol/acetic acid (3:2:3, v/v) solution. The effects of different concentrations of CNFs (0.5-4%) on the important physical properties of the gelatin solution (15%), including rheology, conductivity, and surface tension, were investigated. The apparent viscosity and shear-thinning behavior were increased by increasing the CNF concentration from 0 to 4% at a low shear rate (<10 s-1). CNFs also increased the electrical conductivity and surface tension of the gelatin solution. Scanning electron microscopy (SEM) images revealed uniformly ordered structures with good continuity without fracture or bead formation in all hybrid nanofibers. They also showed that the average diameters of fibers decreased from 216 nm in the pure gelatin nanofibers to 175.39 nm in the hybrid gelatin/CNF (4%) ones. Differential scanning calorimetry (DSC) results showed that CNFs increased Tg, and X-ray diffraction (XRD) analysis showed that the electrospinning process caused the formation of more amorphous structures in the gelatin/CNF hybrid nanofibers. The tensile test indicated that by adding 2% CNFs, the ultimate tensile strength (UTS) and strain at break (SB) of nanofiber mats increased from 4.26 to 10.5 MPa and 3.3% to 6.25%, respectively. The current study indicated that incorporating CNFs at the optimal concentration into a gelatin solution can improve the resulting hybrid nanofibers' morphology, average diameter, and mechanical properties.

Effect of Tea Tree Essential Oil on the Quality, Antioxidant Activity, and Microbiological Safety of Lightly Processed Lily (Lilium brownii var. viridulum) during Storage.

The Lanzhou lily is a regionally distinctive vegetable; the emergence of lightly processed lilies has addressed the inconvenience of consuming fresh lilies. However, the cleaning and impurity removal during the processing of lightly processed lily may strip off its original protective barrier and affect the edible quality. As one of the preservation methods, tea tree essential oil (TTEO) has the characteristics of being green, safe, and efficient preservative properties. This study focused on investigating the effects of different concentrations (25 μL/L, 50 μL/L, and 100 μL/L) of TTEO on the quality and microbiological safety of lightly processed lily. The results showed that compared with the control, appropriate concentrations of TTEO treatment could delay weight loss, improve appearance, firmness, and sensory quality, and maintain microbiological safety with the best effect observed at 50 μL/L. Meanwhile, TTEO treatment induced phenylalanine ammonia-lyase activity, thereby increasing the total phenolic content. Furthermore, TTEO enhanced the superoxide dismutase (SOD) and ascorbate peroxidase (APX) activity, which reduced O2-· production rate and H2O2 content. TTEO inhibited lipoxygenase (LOX) activity, reducing the relative conductivity and malondialdehyde content, thereby delaying lipid peroxidation and quality deterioration. This indicates that TTEO could enhance antioxidant capacity by regulating reactive oxygen species (ROS) metabolism and delay the quality deterioration of lightly processed lily by inhibiting lipid peroxidation.

Ginsenoside Rb1 Inhibits the Proliferation of Lung Cancer Cells by Inducing the Mitochondrial-mediated Apoptosis Pathway.

Lung cancer is one of the more common malignant tumors posing a great threat to human life, and it is very urgent to find safe and effective therapeutic drugs. The antitumor effect of ginsenosides has been reported to be a treatment with a strong effect and a high safety profile. This paper aimed to investigate the inhibitory effect of ginsenoside Rb1 on 95D and NCI-H460 lung cancer cells and its pathway to promote apoptosis. We performed the CCK-8 assay, fluorescence staining assay, flow cytometry, scratch healing assay, and Transwell assay to detect the effects of different concentrations of ginsenoside Rb1 on the antitumor activity of 95D and NCI-H460 cells and Western Blot detected the mechanism of antitumor effect. Ginsenoside Rb1 treatment significantly increased the inhibition and apoptosis rates of 95D and NCIH460 cells and inhibited the cell cycle transition from S phase to G2/M. Rb1 induces apoptosis by altering the levels of P53, Bax, Cyto-c, Caspase-8, Caspase-3, Cleaved Caspase-3, Bcl-2, MMP-2, and MMP-9 proteins and activating the external apoptotic pathway. Ginsenoside Rb1 inhibits proliferation and migration and induces apoptosis of 95D and NCI-H460 lung cancer cells by regulating the mitochondrial apoptotic pathway to achieve antitumor activity.

Cadmium exposure induces changes in gut microbial composition and metabolic function in long-tailed dwarf hamsters, Cricetulus longicaudatus.

Numerous studies have demonstrated that exposure to cadmium disrupts the diversity and composition of the gut microbiota, resulting in damage to organ tissue. However, there remains a lack of comprehensive understanding regarding the broader ecological reality associated with this phenomenon. In this study, we conducted a thorough evaluation of the effects of different concentrations of Cd (6, 12, 24, and 48 mg/L) over a period of 35 consecutive days on the organ viscera and the gut microbiota of long-tailed dwarf hamsters, Cricetulus longicaudatus (Rodentia: Cricetidae), using histopathological analysis, 16S rDNA, and metagenome sequencing. Our findings revealed that the results suggest that Cd exposure induced liver, spleen, and kidney damage, potentially leading to increased intestinal permeability and inflammation. These alterations were accompanied by significant perturbations in the gut microbiota composition, particularly affecting potentially pathogenic bacteria such as Prevotella and Treponema within the gut ecosystem. Consequently, host susceptibility to underlying diseases was heightened due to these changes. Notably though, Cd exposure did not significantly impact the overall structure of the gut microbiota itself. Additionally, Cd exposure induced significant changes in the metabolic functions, with the pathways related to disease and environmental information processing notably enhanced, possibly indicating stronger innate defense mechanisms against external injuries among wild mammals exposed to Cd. This study offers a novel approach to comprehensively evaluate the significant impact of Cd pollution on ecosystems by investigating both structural and functional alterations in the digestive system, as well as disruptions in intestinal flora among wild mammals.

Attenuation of the effect of salinity on redwood (Caesalpinia platyloba) by saline compost

The objective of this research is to evaluate the effect of saline compost on the attenuation of salinity stress in palo colorado (Caesalpinia platyloba). A representative sample of saline soil was taken at Ciudad Universitaria Intercultural, sieved (mesh &lt; 5mm) and composted with cattle manure and Cynodon dactylon garden grass pruning, in a 3:1:1 ratio (stubble: manure: saline soil), for 120 days, the characterization consisted of measuring the bulk density (DA), saturation point, pH and EC of the saturated paste extract. The effect of different concentrations of NaCl on the in vitro germination of palo colorado seeds was evaluated in a randomized complete block design with two substrates (blocks), B1) sand-perlite; B2) sand-perlite-salt compost, five treatments 0 M, 0.3 M, 0.4 M, 0.5 M and 0.6 M of NaCl, eight replicates per treatment. The control irrigation was with distilled water. The plants transference to polyethylene cups was fifteen days after sowing; the cups had the same substrate mixture. The variables measured were survival, leaf biomass and dry root biomass. The EC decreased during composting, from 9.45 dS/m low to 2.4 dS/m; the pH of the samples did not show a significant difference. It is concluded that neither the substrate nor the NaCl concentration significantly influenced the germination of palo colorado seeds, and that the saline compost had no effect on the mitigation of salt stress in palo colorado plants.

Effect of Ziziphus Spina-Christi Leaves on the Level of Testosterone Hormone and ‎Semen Parameters of Iraqi Bucks Semen

The study aimed to assess the effects of different concentrations of Ziziphus spina-Christi (ZSC) leaves on the fertility of buck goats. Twelve buck goats aged 12–15 months with an average body weight of 22±2 kg were divided randomly into three dietary treatments: T1, T2, and T3, each given a basal diet daily plus feeds of 20 g/kg (2%) of ZSC for T1 and 40 g/kg (4%) of ZSC for ‎T2; and T3 was used as the control that consumed a basal diet only. The experiment lasted for 3 months. On days 45 and 90, blood samples were collected for plasma testosterone measurement using an immunochromatographic assay. Additionally, semen samples were collected at the same time points for physical (volume, color, and pH) and microscopic (semen concentration, mass and individual motility percentage, and live-dead sperm percentages) evaluations. ‎ The results showed that ZSC at 4% significantly (P&lt;0.05) increased the testosterone hormone concentration and all physical parameters at 45 and 90 days comparing to 2% ZSC and control ‎groups. ‎Additionally, ZSC at 4% significantly improved (P&lt;0.05) the microscopic parameters at both ‎time periods compared to 2% ZSC and control groups. The present findings revealed that ZSC at 4% diet could enhance goat semen processing and ‎goat fertility enhanced significantly (P&lt;0.05) the testosterone hormone that had higher values ‎compared to ‎‎(2%) of the ZSC control group. Similarly, (4%) of ZSC affected the physical ‎assessment of ‎semen, such as the volume, color, and pH values. Thus, adding ZSC with a high ‎concentration ‎‎(4%) could enhance goat semen processing and goat fertility in general.

New insights into the role of mild hypoxia in regulating neural stem cell characteristics.

The proliferation of neural stem cells (NSCs) is precisely regulated by extracellular and extracellular environmental factors. In situ hypoxia, one of the key factors involved in the regulation of NSC characteristics, has attracted increasing amounts of attention. Numerous studies have demonstrated that hypoxia can significantly promote the formation of neurospheres and the proliferation of NSCs in vitro and that intermittent hypoxia can promote the proliferation of endogenous NSCs in vivo. In this paper, the effects of different concentrations of oxygen on NSC proliferation and differentiation both in vivo and in vitro are reviewed, and the potential applications of hypoxia-preconditioned NSCs, as well as research progress and challenges in the treatment of central nervous system diseases. are further summarized. Here, the critical role of oxygen in the neurogenesis of NSCs is emphasized, and insights into the use of hypoxia to regulate NSC characteristics are provided.

Cultivating Pharmaceutical Potential: Optimising Triacontanol Application to Enhance Linseed’s Attributes under Drought Stress

Background: Triacontanol (TRIA) has been identified as a potent plant growth regulator, demonstrating significant benefits under various stress conditions. However, its specific impact on Linum usitatissimum L. (flax) under drought stress conditions remains underexplored. This study investigates the effects of TRIA on the growth, physiology, and biochemistry of two flax genotypes subjected to drought stress, aiming to enhance our understanding of its potential as a biostimulant for improving crop resilience and productivity. Objective: The primary objective of this study was to evaluate the effects of different concentrations of TRIA on the growth, physiological, and biochemical responses of two flax genotypes under drought stress conditions. We aimed to determine whether TRIA application could mitigate the adverse effects of drought and enhance plant performance. Methods: This study was conducted using two flax genotypes, G-20888 and G-22186. Plants were grown under controlled conditions and subjected to drought stress by maintaining soil moisture at 50% field capacity. TRIA was applied in three concentrations: 0 M (control), 10^-6 M, and 10^-7 M. A completely randomized design was used with three replications for each treatment. Plant growth parameters, including root length, shoot length, plant height, and shoot fresh weight, were measured. Physiological parameters such as chlorophyll content, relative water content (RWC), and electrolyte leakage were assessed. Biochemical analyses included the estimation of antioxidant enzyme activities (superoxide dismutase, catalase, and peroxidase). Data were statistically analyzed using SPSS version 25.0. Results: TRIA application significantly improved root length, shoot length, plant height, and shoot fresh weight in both flax genotypes under drought stress. For genotype G-20888, root length increased from 5.1 cm (control) to 6.8 cm (TRIA 10^-7 M). Shoot length showed a similar trend, with increases from 8.3 cm (control) to 10.1 cm (TRIA 10^-7 M). In G-22186, plant height increased from 70 cm (control) to 82 cm (TRIA 10^-7 M). Chlorophyll content increased by 15%, and RWC by 18% in TRIA-treated plants compared to controls. Antioxidant enzyme activities were also enhanced, with catalase activity increasing by 25% and peroxidase by 30%. Conclusion: TRIA application significantly mitigated the adverse effects of drought stress on flax genotypes by enhancing growth, physiological, and biochemical parameters. The findings suggest that TRIA could be an effective biostimulant for improving drought tolerance in flax, potentially leading to better crop resilience and productivity.

Effect of different concentrations of lysine on the transglutaminase-induced mixed surimi gel properties

Effect of different concentrations of lysine on the transglutaminase-induced mixed surimi gel properties

The Effects of Different Concentrations of Hydrogen-Rich Water on the Growth Performance, Digestive Ability, Antioxidant Capacity, Glucose Metabolism Pathway, mTOR Signaling Pathway, and Gut Microbiota of Largemouth Bass (Micropterus salmoides)

Hydrogen-rich water (HRW) is widely recognized for its growth promoting, antioxidant, and anti-inflammatory properties. However, little is known about the role of HRW in aquaculture. This study aims to investigate how different concentrations of HRW affect the growth performance, digestive ability, antioxidant capacity, mTOR signaling pathway, and gut microbiota of juvenile largemouth bass. We randomly assigned 360 fish (13.73 ± 0.1 g) to three treatments. The control group was maintained in regular water, while the treatment groups were treated with different concentrations of H2 dissolved in water, which were H1 (179.65 ± 31.95 ppb) and H2 (280.65 ± 64.43 ppb), respectively. Through an analysis of the three treatments, it was found that H1 significantly increased the final body weight, weight gain rate, specific growth rate, and survival rate, and reduced the feed conversion ratio (p &lt; 0.05). In addition, the trypsin activity was significantly increased in the intestine (p &lt; 0.05), and the expression of genes related to the glucose metabolism (pk and pepck) and mTOR (tor, akt, s6k1, 4ebp1, and ampka) signaling pathways were significantly increased in the liver in H1 (p &lt; 0.05). The relative abundance of Blautia in the gut microbiota (p &lt; 0.05) was significantly increased in H1. Therefore, these results indicated that H1 can significantly improve growth performance, promote intestinal digestion, activate the glucose metabolism pathway and mTOR signaling pathway, and increase the abundance of beneficial bacteria in the gut of largemouth bass. These findings provided valuable support for the application of HRW to support the healthy aquaculture of largemouth bass.

Effect of different concentrations of sodium dodecyl sulfate (SDS) in cryodiluent on freezability and DNA integrity of bull spermatozoa

Effect of different concentrations of sodium dodecyl sulfate (SDS) in cryodiluent on freezability and DNA integrity of bull spermatozoa

Effect of different concentrations of sufentanil combined with ropivacaine on epidural fever during labor: A single-center retrospective study

Effect of different concentrations of sufentanil combined with ropivacaine on epidural fever during labor: A single-center retrospective study