Effective Extraction Method Research Articles

Green extraction of polysaccharides from Camellia oleifera fruit shell using tailor-made deep eutectic solvents

Camellia oleifera fruit shells are often discarded as byproducts in the C. oleifera industry. There is a general interest in isolating high-value natural products to valorize those fruit shells with green, rapid, and effective extraction methods. This study employed 43 combinations of deep eutectic solvents (DESs) to extract polysaccharides from C. oleifera fruit shells. Two choline chloride-based DESs and a ternary DES with propionic acid and 1,3-butanediol as hydrogen bond donors exhibited relatively high extraction efficiency. The polysaccharide yield reached 15.03 ± 0.35 % under optimized extraction time (55 min), extraction temperature (70 °C), and DES water content (33.33 %). The physicochemical composition and preliminary structure of obtained polysaccharides were characterized. Additionally, DESs-extracted polysaccharides exhibited higher in vitro antioxidant activities and hypoglycemic effects compared to water-extracted polysaccharides. These findings suggested that the optimized DES-assisted extraction method could be a potential approach for polysaccharides extraction from C. oleifera.

Effectiveness of the Use of Vermicompost Larvae of the Black Lion Fly (<i>Hermetia illucens</i>) when Germinating Seeds of Leguminous Crops

The most promising and popular insect in the field of organic waste processing is the black lion fly (Hermetia illucens), whose larvae are unpretentious in food and growing conditions. As a feed, most types of organic waste are suitable for them, which, after being digested by the larva, are enriched with various nutrients and microorganisms useful for the growth and development of plants. Larval excrement is a valuable and environmentally friendly organic fertilizer (biohumus) for growing plants. In order to test the effectiveness of the effect of biohumus of black lion larvae on the process of seed germination in laboratory conditions, liquid extracts of biohumus were obtained by extraction with distilled water and compounds of potassium hydroxide (KOH) and sodium pyrophosphate (Na4P2O7). The most effective extraction method turned out to be the method of treatment with alkali and sodium pyrophosphate, in which the most concentrated extract with a concentration of 2.44% was obtained. All types of extracts of vermicompost have been biotested for germination of seeds of seed peas (Pisum sativum L.) and white lupin (Lupinus albus L.). Compared with the indicators of the control variants with distilled water, all extracts of vermicompost had a stimulating effect on seed germination, accelerating their growth and development. The highest rates were observed in solutions of alkaline pyrophosphate extract with a pH value of 6.7–6.8 at concentrations of 0.01 and 0.05%.

An effective method for the simultaneous extraction of 173 contaminants of emerging concern in freshwater invasive species and its application

A robust and efficient extraction method was developed to detect a broad range of pollutants of emerging interest in three freshwater invasive species: American red crab (Prokambarus clarkii), Asian clam (Corbicula fluminea), and pumpkinseed fish (Lepomis gibbosus). One native species, “petxinot” clam (Anodonta cygnea), was also evaluated. Invasive species are often more resistant to contamination and could be used in biomonitoring studies to assess the effect of contaminants of emerging concern on aquatic ecosystems while preserving potentially threatened native species. So far, most extraction methods developed for this purpose have focused on analyzing fish and generally focus on a limited number of compounds, especially analyzing compounds from the same family. In this sense, we set out to optimize a method that would allow the simultaneous extraction of 87 PhACs, 11 flame retardants, 21 per- and poly-fluoroalkyl substances, and 54 pesticides. The optimized method is based on ultrasound-assisted solvent extraction. Two tests were performed during method development, one to choose the extraction solvent with the best recovery efficiencies and one to select the best clean-up. The analysis was performed by high-performance liquid chromatography coupled to high-resolution mass spectrometry. The method obtained recoveries between 40 and 120% and relative standard deviations of less than 25% for 85% of the analytes in the four validated matrices. Limits of quantification between 0.01 ng g−1 and 22 ng g−1 were obtained. Application of the method on real samples from the Albufera Natural Park of Valencia (Spain) confirmed the presence of contaminants of emerging concern in all samples, such as acetaminophen, hydrochlorothiazide, tramadol, PFOS, carbendazim, and fenthion. PFAS were the group of compounds with the highest mean concentrations. C. fluminea was the species with the highest detection frequency, and P. clarkii had the highest average concentrations, so its use is prioritized for biomonitoring studies.Graphical abstract

Extraction of phycocyanin from Spirulina using Deep Eutectic Solvent Liquid Biphasic System

BackgroundPhycocyanin (PC) is an intracellular protein produced in Spirulina sp. and is widely used as a natural dye in the cosmetic, food, and supplements industries. But the extraction of phycocyanin was limit by properties of proteins and technologies. Here, deep eutectic solvents were found to be an effective method of extraction, circumventing any usage of harmful organic solvents. MethodsIn particular, we explored the use of DES/K2HPO4 liquid biphasic system (LBS) as the extraction medium, where the extracted PC from the cell in bottom phosphate aqueous phase is selectively transferred to the top DES phase. FindingsAmong the different DES, the ChCl-Urea and ChCl-Glycerol showed the highest extraction efficiencies and yields, but the Glycerol synthesized DES has bad mass diffusion problems. Through continuous single-factor optimization, we finally achieved the best extraction efficiency and yield of 94.2% and 92.0%, respectively, using ChCl-Urea/K2HPO4. This proposed solution has the potential to be produced on a large scale and is environmentally friendly for the extraction of phycocyanin.

Upgrading the durability of perishable wood species using extractives from side streams of durable wood sawmill operations: a review

Abstract Natural durability is one of the most rated features in wood end-use applications. In fact, several precious native tropical wood species produce timber of high natural durability, which is also related to long service life even for the highest hazard classes. However, selective logging is driving the existing volume of this group of species to near extinction. The remainder of the alternative species produces perishable timbers, which require synthetic chemical protection to prolong their service life but with detrimental effects on humans and the environment. Therefore, transferable durability has emerged as an alternative to gradually substitute traditional wood preservatives. From this approach, extractives from naturally durable wood species are removed and transferred to the non-durable wood species as an alternative environmental-friendly option for wood protection. Indeed, extractives from durable wood species have proven to have a deterrent effect on fungi, bacteria and termites and could be used to protect perishable wood species. Thus, this review aims to assess the prospects of developing environmentally friendly wood preservatives based on extractives sourced from highly natural, durable wood species to treat and add value to the group of perishable timbers. A step-wise analysis offers insights and challenges on (i) potential sources of extractives; (ii) effective extraction methods; (iii) extractive-based preservative formulation; and (iv) effective treatment methods for better preservative fixation for better wood protection. Accounts about the way forward for the development of extractive-based wood preservatives are also presented.

A novel simplified method for extraction of microplastic particles from face scrub and laundry wastewater

Microplastic pollution in different environmental matrices is a serious concern in the recent times. Personal care products and washing of synthetic fabrics are some of the main sources of microplastic pollution. In this work, a novel simplified, effective and sustainable method for extraction of microplastic particles from face scrub and laundry wastewater was developed. Different parameters affecting the extraction were analysed and the extraction process was optimised. The extraction efficiency of the proposed method was found to be ~ 94.1 ± 1.65%, which was slightly better than the previously available method with an advantage of ease in extraction and lesser time and resource consuming. The developed method was used to demonstrate the extraction of microplastic particles from 12 face scrub samples with different brands. It was found that the samples contained microplastic particles of varying size. The physical and chemical structure intactness of microplastic particles during the extraction was also analysed and found to be acceptable. The developed extraction method was also applied for the extraction of microfibers from the laundry wastewater. It was found that this proposed method is suitable to make the cleaner extracted samples for an easy and more effective qualitative and quantitative analysis of MPs.

Degradation of selected organophosphorus pesticide residues and their pre harvest interval determination in hyacinth bean grown commercially in Bangladesh

The consumers are very much concerned about food safety. To ensure safe food for the consumers, it is essential to follow the pre harvest interval (PHI) of pesticides. Therefore, in order to ascertain the pre-harvest interval of fenitrothion and diazinon in hyacinth bean in the environmental conditions of Bangladesh, this study was started. The chosen organophosphorus insecticides (fenitrothion and diazinon) were sprayed with the recommended dose (1.5 ml/L of water) in a field trial at the Entomology Division of BARI, Gazipur. At 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 12 days after spray (DAS), samples were taken. The Gas chromatography (GC) with a Flame Thermionic Detector (FTD) was used to quantify the residues after the samples were prepared using a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction method. Fenitrothion residue was found up to 8 DAS, and at 7 DAS (0.018 mg/Kg), the level of residue was above the MRL. At 8 DAS, the fenitrothion residue was 0.007 mg/kg, which was below MRL. Pre-harvest interval (PHI) for fenitrothion was determined at 8 DAS, and no residue was found after 9 DAS. Diazinon residue was found up to 9 DAS, with the level of residue above the MRL at 8 DAS (0.016 mg/Kg). At 9 DAS, the diazinon residue was 0.007 mg/kg, which was below MRL. Pre-harvest interval (PHI) was determined for diazinon at 9 DAS, and as of 10 DAS, no residue was found. Asian Australas. J. Biosci. Biotechnol. 2023, 8 (2), 30-37

Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column

Process development for efficient pectin extraction from tobacco residues and its characterisation

AbstractThis study presents a method for effective pectin extraction from the laminae of three tobacco varieties as a means of biomass valorisation. Two pre-treatment methods (cold ethanol vs. accelerated solvent extraction [ASE] with ethanol) were compared for their capacities to produce a high pectin yield. Enzymatic extraction of pectin was also tested as a green extraction procedure and compared to the acid extraction approach. The optimisation experiments revealed that cold ethanol extraction followed by acid hydrolysis is the most convenient method for pectin extraction; the optimal set of conditions for hydrolysis were identified as 90°C, pH 1.5, and 4 h of extraction. Applying these optimised conditions to the three Nicotiana rustica tobacco varieties yielded pectin recoveries of 66.2%, 57.8%, and 56.7% from the NRT63, Bakoum Miena, and NRT61 samples, respectively. Tobacco pectins were found to have a medium molecular weight and low methoxy content. These results highlight the potential of tobacco residues as feedstock for to produce pectin with dietary applications.

Advances and Developments in Monitoring and Inversion of the Biochemical Information of Crop Nutrients Based on Hyperspectral Technology

Crop nutrient biochemical information (mainly including chlorophyll class and nutrient elements mainly nitrogen, phosphorus and potassium) is an important basis for revealing crop growth and development patterns and their relationship with the environment. Hyperspectral technology has been rapidly developed and applied in crop nutrient biochemical information monitoring research. This paper firstly describes the theoretical basis of hyperspectral technology for monitoring crop nutrients and biochemical information. Then, the research progress of hyperspectral technology in monitoring nutrient and biochemical information of crops in different growth periods or different growth environments is outlined. Meanwhile, the shortcomings of the current technology in these research directions and the future research trends are discussed. Finally, the modeling methods for building crop nutrient biochemical information monitoring models by applying hyperspectral data are systematically outlined. And the effects of different spectral pre-processing methods, spectral effective information extraction methods and modeling algorithms on the accuracy of monitoring models are analyzed. On this basis, the challenges and prospects of hyperspectral technology in monitoring crop nutrient biochemical information are presented, aiming to provide relevant theoretical basis and technical reference for the research related to monitoring and inversion of crop physiological parameters based on hyperspectral technology.

An effective and low-cost method for DNA extraction from Rheum species (A medicinal herb) without using liquid nitrogen for RAPD/ISSR Study

Rheum is an important medicinal plant belonging to family Polygonaceae and is found to have anti-microbial, anti-purgative and anti-cancer properties. The objective of the present study is to standardize an effective and low-cost method for the extraction of DNA from the young juvenile leaves of Rheum plant obtained from the Uttarakhand region. The protocol involved the extraction of DNA using a modified CTAB method without the use of any liquid nitrogen. The concentration of NaCl used in extraction buffer was slightly higher (1.4M) to remove polysaccharides and varying ratios of Beta-mercaptoethanol used to remove the secondary metabolites and keep them away from interfering the extraction process. The absorbance ratio of the extracted DNA at A260/280 nm was within the permissible range of 1.7-2.0, indicating the purity of DNA and showing that the DNA is contamination free. Its concentration was approximately 1485 ng/μl and it was found suitable for molecular-based study.

Optical edge-enhanced imaging based on dielectric metasurfaces

Optical edge-enhanced imaging is an effective method for boundary extraction, and it is also a cutting-edge technology for object detection in image processing. With the rapid development of metamaterial and metasurface fields, the miniaturization and lightweight of optical devices have reached a new scale. At present, the experimental research of optical edge-enhanced imaging using metasurface is insufficient and lags behind the theoretical research. In this paper, we propose optical edge-enhanced imaging based on a Huygens phase metasurface in the near-infrared (NIR). The entire optical edge-enhanced process is simulated, and the feasibility of the method is verified theoretically. Then, the edge-enhanced of numbers, letters, and biological tissues is carried out experimentally, and the results are consistent with the simulation results. This study proposes a near-infrared dielectric metasurface-based edge-enhanced imaging that can find important applications in compact optical platforms such as image processing.

Influences of Trypsin Pretreatment on the Structures, Composition, and Functional Characteristics of Skin Gelatin of Tilapia, Grass Carp, and Sea Perch.

Fish skin gelatin is an important functional product in the food, cosmetics, and biomedicine industries, and establishing a green and effective fish skin gelatin extraction method is an effective way to obtain high-quality gelatin and improve its production efficiency. In this study, a trypsin method was used to extract the skin gelatin of sea perch, tilapia, and grass carp, and the microstructures of skin gelatin of these three fish species were analyzed, with such functional characteristics as thermal stability, gel strength, and emulsifying properties measured. The study results show that the skin gelatin of sea perch and tilapia obtained through the trypsin method has a relatively big molecular mass, a dense network structure, and a stable trihelix conformation. In addition, the skin gelatin of these three fish species has a relatively high β-turn content in the secondary structure, good gel strength, and water absorption properties. The compositions of the collagen-associated proteins in the skin gelatins of these three fish species extracted with the trypsin method are significantly different from each other, with positive effects of decorin and biglycan on the stability of the network structure of gelatin and a certain damaging effect of metalloendopeptidase on the network structure of gelatin. The skin gelatin of tilapia has high thermal stability and good emulsifying performance. Therefore, this gelatin type has bright application prospects in such fields as food processing, cosmetics, and drug development. In contrast, the skin gelatin of grass carp has poor functional properties. Therefore, there are significant differences among the structures and functions of skin gelatin extracted from different kinds of fish through the trypsin method. This finding has provided a useful reference for the production of customized fish gelatin according to demand.

Effective Event Extraction Method via Enhanced Graph Convolutional Network Indication with Hierarchical Argument Selection Strategy

Effective Event Extraction Method via Enhanced Graph Convolutional Network Indication with Hierarchical Argument Selection Strategy

An Effective and Low-Cost Method for Genomic DNA Extraction from Cyclanthera Pedata Species (A Nutraceutical Plant) without Liquid Nitrogen for Inter Simple Sequence Repeat Analyses

An Effective and Low-Cost Method for Genomic DNA Extraction from Cyclanthera Pedata Species (A Nutraceutical Plant) without Liquid Nitrogen for Inter Simple Sequence Repeat Analyses

Key Frame Extraction Based on Real-Time Person Availability Using YOLO

Keyframe extraction plays a crucial role in summarizing lengthy videos, particularly in the context of surveillance footage with a fixed field of view that records events over extended periods. The process of manually reviewing such videos can be time-consuming and challenging to extract essential information effectively. To address this issue, a study was conducted to evaluate four distinct methods for keyframe extraction, with the aim of determining the most suitable approach for creating a people-based dataset. The four methods assessed in the study were absolute difference, entropy, optical flow, and object detection-based video summarization using the YOLO (You Only Look Once) algorithm. Each method offers a unique approach to identify keyframes that encapsulate critical instances within the video footage. Among all the four evaluated methods, the object detection-based video summarization approach stood out as particularly promising. This method employed the YOLO algorithm, which utilizes advanced object detection techniques to identify and track people within the video frames. With this approach only fewest numbers of frames are extracted but still capturing all the relevant instances featuring people. The results of this study suggest that object detection-based video summarization using the YOLO algorithm is a highly effective method for keyframe extraction in surveillance videos. By significantly reducing the number of frames while preserving all relevant instances, this approach offers a time-efficient solution for reviewing and analyzing extensive video footage, ultimately facilitating the creation of a people-based dataset for further research and applications in various domains.

Highly Effective Extraction of Gigantol from Dendrobium officinale Using the Ultrasonic-assisted Dry Grinding

Background Dendrobium officinale is a valuable medicinal food plant in China, and its component gigantol has various biological activities. However, due to the low extraction rate of gigantol, its application in the functional food industry is limited. This research aimed to explore an effective gigantol extraction method. Results Ultrasonic-assisted dry grinding extraction of gigantol from D. officinale almost reached completion after 15 min. The extraction rate of gigantol reached 65%, which was higher than that of the conventional heating refluxing method of extraction. Most cells were broken, and particle size was approximately 410 nm when D. officinale was ground for 15 min. Conclusion The results indicated that ultrasonic-assisted dry grinding is a highly effective gigantol extraction method that is valuable for the efficient use and low-cost extraction of gigantol from the medicinal food plant D. officinale. Furthermore, this method will be beneficial for the future application of gigantol in the functional food industry.

Optimization of Soxhlet Extraction of Candlenut Oil (Aleurites moluccana (L.) willd) Using Factorial Experimental Design Level 23

Candlenut (Aleurites moluccana (L.) wild) is a plant of the Euphorbiceae family. Candlenut oil is obtained by extracting the oil content in candlenut seeds. Soxhlation is an effective method for solvent extraction, ensuring continuous contact between the solvent and the material to extract the oil efficiently while maintaining the purity of the solvent. In this study, optimization of candlenut oil extraction was carried out using the factorial experimental design level 23. Using this method, it can be determined that the extraction time is the most influential variable in the candlenut oil extraction process. The results showed that the optimum operating conditions were an extraction time of 185 min, a candlenut seed powder size of 20 mesh, and a ratio of material-solvent ratio of 1:6 g/g, resulting in an oil yield of 43.2%. The analysis of candlenut oil conducted at an extraction time of 185 min revealed the following results: a refractive index value of 1.4736, a moisture content of 0.08% bb/b, which complies with the standard SNI 01-4462-1998 for candlenut oil, a viscosity of 15.47 Cp, and a density of 0.869 g/mL. The analysis of the free fatty acid number of 2.4%bb/b and saponification number of 181.55 mg KOH/g is not consistent with SNI 01-4462-1998 candlenut oil.

Microextraction by packed sorbent of some β-blocker drugs with chitosan@mof-199 bio-composite in human saliva, plasma, and urine samples

The current study introduces microextraction by packed sorbent (MEPS) to extract three beta-blocker drugs (propranolol, atenolol, and betaxolol) from biological samples. The separation and detection of the drugs were performed by high performance liquid chromatography followed by UV detection. A green approach was applied for synthesizing chitosan@MOF-199 bio-composite, which was packed into the initial part of a metal spinal (22 gage). The effective parameters on the adsorption and desorption efficiencies, including sample solution pH, eluent flow rate, cycle numbers, type and volume of eluent solvent were evaluated and optimized. Under optimal conditions linear ranges (LRs = 5–600 µg L−1), limits of detection (LODs = 1.5–4.5 µg L−1), and relative standard deviations (RSDs% = 4.7 −5.3% with three replicates and concentration of 100 µg L−1) were obtained. Relative recoveries (RR%) for plasma (77–99%), saliva (81–108%), and urine (80–112%) samples were obtained. In this study, the drug release profile of propranolol in urine was evaluated. The results showed that the highest amount of propranolol is released 4 h after taking the drug. Based on the obtained results, this is an effective, fast, sensitive, reproducible, green, and user-friendly method for beta-blocker drug extraction in biological samples.

PHYSIOCHEMICAL CHARACTERISTICS AND FATTY ACIDS COMPOSITION OF MORINGA OLEIFERA OIL OF FAR NORTH CAMEROON

In this work, a comparative study on Moringa oleifera (MO) oil extraction techniques were conducted. MO seeds were collected from the Far North Region of Cameroon and oil was extracted using Soxhlet (SO), Ultrasonic (US) and Cold press (PR) methods. Physicochemical characteristics and fatty acid composition were determined for each MO oil extracted. The SO seems to be the effective method for oil extraction with a yield equal to 38.94 ± 0.28%, followed by US 35.11 ± 1.45% and at least PR (23.00 ± 1.00%). Moreover, myristic and lignoceric acids were found at low concentration in all extracted oil. However, the unsaturated fatty acid composition of the extracted oils follows PR>US>SO order. Oleic acid was the main unsaturated fatty acid with high rate obtained using PR and SO extractions (75.37%, 75.31% respectively) which is not very different from that obtained by US extraction (74.85). A higher value of acidity was noted in PR extraction method and lower oxidative state value was noted in PR extracted oil comparing to US and SO methods. No major differences were observed for the other physic-chemical parameters. Major and minor saturated fatty acids were palmitic and myristic acids, respectively. Lower total saturated fatty acids were recorded in PR extracted MO oil. We found also linoleic and linolenic acids in the group of unsaturated fatty acids of MO oil.