Effect Of Ultrasound Waves Research Articles

The enhancement of therapeutic effects of low-intensity ultrasound with spiky and spherical gold nanoparticles on CT26 cell line; an in vitro study

Introduction: The utilization of combination therapy has gained attention in the medical field as it can enhance treatment efficacy while minimizing side effects. Gold nanoparticles (AuNPs) have been identified as a promising candidate for enhancing the bioeffects of ultrasound (US) waves. Objectives: The primary objective of this study was to examine the impact of US irradiation when combined with spiky and spherical AuNPs on CT26 cells. Materials and Methods: Following a tailored composition, we devised and produced AuNPs with spiky and spherical morphologies. The properties of these nanoparticles were assessed using dynamic light scattering (DLS), voltammetry, and transmission electron microscopy (TEM). The CT26 cells were treated with the two types of nanoparticles and subsequently exposed to US radiation (1 W/cm2 , 1 MHz, and 10 minutes). The cytotoxic effects of various therapeutic interventions were assessed using MTT and flow cytometry assays. Results: This study’s findings indicate that nanoparticles’ morphology played a significant role in influencing the antitumor effects of US. Specifically, it was observed that the presence of spiky nanoparticles, when subjected to US, resulted in a significantly higher rate of cell death compared to spherical nanoparticles (P<0.001). Additionally, it was observed that the temperature changes induced by the spiky nanoparticles were comparatively lower. This observation implies that nanoparticles with spiky morphology enhance the non-thermal effects of US waves. Conclusion: The outcomes demonstrate a noteworthy augmentation in the biological impacts, specifically the non-thermal effects of US waves when employed in conjunction with spiky nanoparticles.

Effects of ultrasound waves on rat liver mitochondria

Succinate dehydrogenase has a wide range of properties in plant and animal cells and is distributed throughout the inner membrane of mitochondria, therefore it is the enzyme without which energy metabolism cannot occur. The goal of our research was to investigate the effect of ultrasonic waves on the intensity of lipid peroxidation, activity of succinate dehydrogenase and cytochrome c oxidase in rat liver mitochondria, as well as antioxidant effect of mulberry leaf extract and biosep oil extract. Study involved white lab rats weighing 180-200 grams. The ultrasound device used was Mindrey DP-50 Vet. The work was based on the following methods: Schneider differential centrifugation, spectrophotometry, chromatography, pH-metry, photometry. The results of the study explain the elucidation of the mechanisms of damage to liver tissue by ultrasonic waves and the correction of the mitochondrial dysfunction caused by it with the extract of mulberry leaves and the oil extract of biosep. It was revealed that changes in lipid peroxidation led to disruption of the functional activity of membrane-bound cytochrome c oxidase and succinate dehydrogenase.

Review of Adsorption Studies for Contaminant Removal from Wastewater Using Molecular Simulation

In recent years, simulation studies have emerged as valuable tools for understanding processes. In particular, molecular dynamic simulations hold great significance when it comes to the adsorption process. However, comprehensive studies on molecular simulations of adsorption processes using different adsorbents are scarcely available for wastewater treatment covering different contaminants and pollutants. Hence, in this review, we organized the available information on various aspects of the adsorption phenomenon that were realized using molecular simulations for a broad range of potentially effective adsorbents applied in the removal of contaminants from wastewater. This review was compiled for adsorbents under five major categories: (1) carbon-based, (2) oxides and hydroxides, (3) zeolites, (4) metal–organic frameworks and (5) clay. From the review, it was found that simulation studies help us understand various parameters such as binding energy, Gibbs free energy, electrostatic field, ultrasound waves and binding ability for adsorption. Moreover, from the review of recent simulation studies, the effect of ultrasound waves and the electrostatic field was elucidated, which promoted the adsorption capacity. This review can assist in the screening of classified adsorbents for wastewater treatment using a fast and cheap approach while helping us understand the adsorption process from an atomistic perspective.

Experimental and theoretical feasibility study of methanol application for Echium oil-based biodiesel production

In this study, the effect of ultrasound waves on biodiesel production from methanol and Echium oil is evaluated experimentally under different operating conditions. A two-step esterification–transesterification process was applied to improve the biodiesel quality, and, in order to optimize the reaction conditions, the effect of variables such as methanol to oil molar ratio, KOH catalyst concentration and temperature were experimentally investigated. The results showed that the optimum biodiesel yield (97.2%) could be obtained at a catalyst concentration of 1.875% w.t, feed molar ratio equal to 9.65, and at 50 °C of operating temperature. The acid value, saponification numbers, iodine value, density, kinematic viscosity, fire point, cloud point and flash point were measured and compared with ASTM D6751 and EN 14214 standards. The results of the analysis showed that the produced biodiesel is a good alternative to fossil fuels. The results demonstrate that methanol and Echium Oil can be used as a proper feedstock for biofuel production. Furthermore, the kinetic study of transesterification is carried out for Echium oil production and the reaction rate constant and activation energy were determined. Finally, the sizing calculations and cost estimation of the Echium oil-based biodiesel production were investigated.

Evaluation of promoted Ni-based nanocatalysts in wall-coated microchannel reactor on the dry reforming of methane and effect of ultrasound waves on physiochemical properties of synthesized nanocatalysts

Abstract The deactivation of nickel catalysts in the dry reforming of methane (DRM) process has been one of the issues of interest to researchers. In this research, the effect of active phase and support promoter uses and synthesis method on synthesized Ni–Co/Al2O3–MgO nanocatalysts efficiency in wall coated microreactor on dry reforming of methane process studied. To determine the characteristics of the synthesized samples, XRD, BET, FESEM, and Ft-IR analyses have been performed. Analyses show that the use of ultrasound waves in the synthesis of catalysts improves the catalyst surface morphology so that about 82% of the particles of the synthesized sample are smaller than 100 nm and , increases the specific surface area to an average of 10%, and makes its structure smaller. Also, the total pore volume on the surface of the samples also shows a 10% increase. The use of promoters increases the catalyst activity and makes it more stable up to 18 h on stream. The use of a wall-coated microreactor improves heat transfer, easier access of reactants to active sites, no pressure drop, and higher activity than a conventional U-type fixed bed reactor. Nanocatalysts with Ni/Co = 5 and Al/Mg = 5 has shown the highest and most stable activity throughout the temperature range in the DRM process.

Modeling of an Ultrasound System in Targeted Drug Delivery to Abdominal Aortic Aneurysm: A Patient-Specific in Silico Study Based on Ligand-Receptor Binding.

Targeted drug delivery methods have shown a significant impact on enhancing drug delivery efficiency and reducing drug side effects. While various stimuli have been used to promote the drug delivery process, applying ultrasound (US) waves to control drug particles through the human body, noninvasively, has drawn the scientist's attention. However, microcarriers delivery reaches the aneurysmal artery by US waves that exert volumetric forces on blood, and drug carriers, which can therefore affect blood flow patterns and movement pathways of drug carriers, have not yet been studied. In this study, we developed a 3-D patient-specific model of abdominal aortic aneurysm (AAA) to evaluate the effect of US waves in enhancing the drug-containing microbubbles (MBs) adhered on the AAA lumen through ligand-receptor binding. Thus, a focused US (FUS) transducer with a resonance frequency of ~1.1 MHz was added to the geometry. Then, the surface density of MBs (SDM) adhered on the AAA lumen was calculated at peak acoustic pressure of ~1.1, ~2.2, and ~4.3 MPa. Results indicated that increasing the US pressure had a significant impact on improving the MBs adhered to the intended wall, whereby US waves with the maximum pressure of ~4.3 MPa could enhance ~1- [Formula: see text] MBs adhesion ~98% relative to not using the waves. While US waves have the advantage of more SDM adhered to the whole artery wall, they adversely affect the SDM adhered on the critical wall of the abdominal aorta. Furthermore, when the US strength goes up, a reduction occurs in the SDM adhered. This reduction is higher for smaller MBs, which is the mentioned MBs' size and US strength reduced SDM adhesion by about ~50% relative to systemic injection. Therefore, it can be concluded that drug delivery using the US field increases the SDM adhered to the whole AAA wall and decreases the SDM adhered to the critical wall of AAA.

The Effects of Ultrasound Wave on the Extraction of Proanthocyanidins from Red Sorghum Grain Using Green Solvent and a Kinetics Model of the Extraction

Ultrasound-Assisted Extraction (UAE) was applied to enhance the bioactive compound extraction from the intact red sorghum grains using water as a green solvent that is relatively more available, safe, and less contaminant to the product application. The UAE performance of the ultrasonic probe with 60, 70, and 80% amplitude were compared to evaluate the effect of ultrasound amplitude. A mechanistic model was applied for quantitative evaluation of the UAE performance and prediction of the concentration of total phenolic (TP) and proanthocyanidin (PC) in the extract during the extraction. This research evaluated the effect of amplitude on the extraction rate, the parameters in the model, and the predicted TP and PC concentration from the mechanistic model approach. The wave enhanced the extraction by its propagation, cavitation bubble, and pericarp destruction. The mass transfer coefficient was stretched between 1.271×10–2 - 1.345×10–2 cm–1/minutes for TP and 5.979×10–3 - 8.262×10–3 cm–1/minutes for PC, while the distribution coefficient was 2.222×10–2 -2.248×10–2 grain/cm–3 solvent for TP and 6.739×10–2 - 7.946×10–2 grain/cm–3 solvent for PC. The highest performance resulted from 80% amplitude. The model worked precisely to predict the concentration of TP and PC of UAE, illustrating the amplitude’s effect in enhancing the UAE from its parameter values.

How safe is it to use ultrasound in prenatal medicine? Facts and contradictions - Part 2 - Laboratory experiments regarding non-thermal effects and epidemiological studies.

The first part of this CME article (issue 5/20) provided a detailed examination of the biophysical effects of ultrasound waves, the exposure values, and in particular the thermal effect. In vivo and in vitro measurements have shown that the temperature increase in tissue associated with B-mode ultrasound is far too low to pose a potential risk. Even experiments with exposure values in the range of pulsed Doppler have shown that temperature increases of over 1.5 °C can only occur in areas in direct contact with the probe, thus making a limited exposure time particularly in the case of transvaginal application advisable. The second part of this CME article describes various laboratory and animal experiments for evaluating non-thermal effects and also presents the most important epidemiological studies in the last 30 years in the form of an overview and review. In addition to direct insonation of isolated cells to examine possible mutagenic effects, the blood of patients exposed in vivo to ultrasound was also analyzed in multiple experiments. Reproducible chromosome aberrations could not be found in any of the studies. In contrast, many experiments on pregnant rodents showed some significant complications, such as abortion, deformities, and behavioral disorders. As in the case of thermal effects, the results of these experiments indicate the presence of an intensity- or pressure-dependent effect threshold. Numerous epidemiological studies examining possible short-term and long-term consequences after intrauterine ultrasound exposure are available with the most important studies being discussed in the following. In contrast to information presented incorrectly in the secondary literature and in the lay press, health problems could not be seen in the children observed in the postpartum period in any of these studies.

Electro-sonical deposition of nanostructured Sb2Se3 films for optoelectronic applications

In this study, a film of antimony selenide (Sb2Se3) was deposited on the Fluorine-doped tin oxide (FTO) as a substrate via electrodeposition at the potential of −0.55 V and the temperature of 25 °C. The effect of ultrasound waves during electrodeposition was also investigated at different times. The structural, morphological, optical, optoelectronic, and temperature dependence of the electrical resistance of the samples was investigated. The results showed formation of the polycrystalline phase of orthorhombic Sb2Se3. It was observed that when the ultrasound waves were used, the crystallite size (grain) and energy band gap decreased. It was found that in samples with a specified configuration, the intensity of the photocurrent decreased with increasing deposition time. Additionally, the anodic and cathodic photocurrent behaviors of the samples depended on the type and location of the electrode connection. Furthermore, the electrical resistance of the samples decreased with increasing temperature. In the sample with the lowest deposition time, conductivity and electrical activation energy were reduced by applying ultrasound waves, but they were increased by increasing the deposition time.

Bio-efficacy of ultrasound exposure against immature stages of common house mosquitoes under laboratory conditions

Purpose: In southern Iran with a tropical climate, the above and underground cisterns of drinking water are the main habitats for immature stages of mosquitoes. The local people do not agree with using calcium hypochlorite granules in the water cisterns due to the change of water taste. Following the increased worldwide interest in using ultrasound technology for water disinfection, this study was conducted to assess the possible dual effects of ultrasound waves against immature mosquitoes.Materials and methods: Combinations of four power levels, two frequencies, three temperatures, and eight exposure times were applied to immature Culex pipiens. The exposure chamber of immature mosquitoes was the disposable cups placed inside a sonication bath containing tap water.Results: The mortality rate of immature mosquitos was 85–91% at 10–25 W, 30 °C and 130 kHz. The most effective ultrasound regimen for causing immature mortality included a fixed frequency of 130 kHz, a temperature of 30 °C, and a power level of 20–25 W. The lowest efficacy was observed in the egg stage. The median lethal time (LT50) and ninety percent lethal time (LT90) of sonication was 5.9 ± 0.6 min and 1.6 ± 0.1 min at 30 °C and a fixed frequency of 130 kHz.Conclusions: Evidence indicated effective ultrasound irradiation against immature mosquitoes in the water. This study provides a clear scientific basis for larvicidal properties induced by ultrasound emissions at laboratory conditions. The findings could be used for setting the ultrasound devices in the field investigations for achieving desired effectiveness.

English

There are many processes the oil industry used to recover more oil from the reservoir after the natural energy is depleted. In this research work, ultrasound energy has been tested as a method of improving oil recovery after waterflooding. The method used in this research work was a numerical simulation using MATLAB Reservoir Simulation Toolbox (MRST-2017a). Ultrasound energy source equation was incorporated into the residual energy equation in the MRST. Acoustic attenuation pressure equation was modelled as part of the residual pressure equation in the MRST. Effect of ultrasound waves on pore wall deformation was also modelled from waves stress equation. Two-phase (oil/water) black oil model was used to simulate fluid flow. Results obtained from the simulation runs show that the ultrasound energy has an impact on reservoir performance i.e. increase in fluid flowrates and pore pressure and temperature rise which led to oil viscosity reduction and consequently improve mobility ratio. However, attenuation of ultrasound energy increases with propagation distance, but this effect was compensated by increasing the intensity of the ultrasound power. Though, high power intensity induced stress on the reservoir pore throat. Results obtained also show that high porosity and permeability values affect the performance of the acoustic energy basically due to an increase in the rate of waves absorption. Key words: Ultrasound energy, improved oil recovery, reservoir simulation toolbox, MATLAB. &nbsp

Effect of ultrasound waves on hematological parameters, behavior and conduction system of heart in vitro in BALB/c strain mouse

In this study research was carried out on 5 male adult BALB/c mouse with a weight of 18±5 g. The temperature was at 22±2 °C and animals were kept under the condition of 12 h of light and 12 h of darkness (light on at 08:00 am) and free access to water and standard diet. All 5 mice were affected by three different harmonic oscillations one time and after 24 hours 4 of them were affected by the same oscillations (Fig. 1) and one was placed separately for control. At the end of the experiment, after euthanasia using CO2 chamber blood samples were collected by cardiac puncture. We noticed that ultrasound waves 20 MHz changed mice red blood cells structure and we found pathological forms of erythrocytes. Analysing morphological structure of erythrocytes were determined 46 abnormal red blood structure forms and atypical cells (30 codocytes, 8 stomatocytes and 8 acantocytes). Moreover, we determined that changed some normal behavior characteristics and increased grooming process.

Ultrasound‐assisted synthesis of porous Mg(OH) 2 nanostructures using hypersaline brine

In this work, synthesis and formation of porous magnesium hydroxide (MH) nanostructures were investigated by chemical precipitation and sonochemical method using hypersaline brine containing large amounts of CaCl 2 , MgCl 2 , NaCl and KCl. The effect of ultrasound waves was investigated on the morpho-structural, surface and thermal properties were investigated by means of X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray spectrometer, Brunauer-Emmett-Teller and thermogravimetry-differential thermal analysis techniques. It was indicated that ultrasound waves decreased grain and particle sizes of the sample which synthesised by sonochemical route (MH-U). The MH-U sample had a higher surface area, lower particle size, better particle distribution and lower transition temperature of MH to MgO.

Effect of Sonication on The Mechanical Properties of Organic Mixed Grain Bread

AbstractThe aim of this study was to determine the effect of ultrasound waves on mechanical properties of organic bread. Mixed grain bread baked at a local bakery according to its original recipe was the material subjected to testing. Prior to baking, the dough was sonicated at frequencies of 20 kHz and 40 kHz for 3 minutes, and at a frequency of 100 kHz for 3 minutes and 6 minutes. The mechanical properties of the bread (compression test, penetration test and TPA) were examined after 24 h, 48 h and 72 h following the baking process. The maximum work obtained in the compression test after 24 h following the baking ranged from 616 N to 668 N. On the next day following the baking, the maximum work value ranged from 750 N to 898 N, while after 72 hours, it ranged from 988 N to 1135 N. In the penetration test, the following results were obtained: after 24 hours, the force value ranged from 2.71 N to 3.17 N; after 48 hours following the baking, it ranged from 4.90 N to 6.35 N; and after 72 hours following the baking, from 3.98 N to 6.88 N. As regards elasticity, the obtained results fell within the following ranges: after 24 hours following the baking, from 0.53 to 0.58; after 48 hours following the baking, from 0.43 to 0.55; and after 72 hours following the baking, from 0.40 to 0.45. Gumminess measured after 24 hours following the baking ranged from 8.75 N to 18.71 N; after 48 hours, from 10.83 N to 15.48 N; and after 72 hours, the gumminess values ranged from 15.57 N to 19.06 N. As regards chewiness, the following results were obtained: after 24 hours following the baking, from 8.00 N to 17.59 N; after 48 hours following the baking, from 9.77 N to 13.87 N; and after 72 hours following the baking, from 12.56 N to 16.85 N. The frequencies and durations of sonication applied changed no mechanical properties of the tested bread.

An investigation on effect of ultrasound waves on sludge treatment

Sludge treatment in wastewater treatment plants are one of the most difficult challenges. The purpose of this study is to determine the effect of ultrasonic waves on soluble chemical oxygen demand (SCOD), temperature and pH of the sludge during improving the sludge dewatering properties. In this research variables include ultrasound density and Time. With the increase of time, the SCOD also increased, which is due to increasing exposure of cells and microorganisms to sonification. This Enhancing Contacts with ultrasound waves leads to increasing cell wall collapse and the withdrawal of intracellular water, proteins and nutrients into the sludge. Also with the increase of ultrasound density, the rate of SCOD increase has raised. This means an increase of SCOD of the sludge and it is due to the higher import of energy and production of nano-bubbles and damage of cells and biological flocs. By increasing the ultrasound density and sonification time, the temperature was significantly increased.

The effects of ultrasound waves on yield, texture and some qualitative characteristics of cheese

In this study, the effects of bath and probe ultrasound treatments were investigated on yield, texture (hardness, adhesion, cohesion, springiness and chewiness), pH and moisture content of fresh white cheese. The times 2, 4, 6 minutes and 5, 10, 15 minutes were used in probe treatment (frequency 20 kHz) and bath treatment (frequency 37 kHz), respectively, at temperatures of 40, 50 and 60°C in two stages (raw cow milk and cheese matrix). The results showed that applying ultrasound treatment significantly (P

Effect of ultrasound waves against infection with giardiasis in Rattus norvegicus rats.

Effect of ultrasound waves against infection with giardiasis in Rattus norvegicus rats.

Increasing vaccine production using pulsed ultrasound waves.

Vaccination is a safe and effective approach to prevent deadly diseases. To increase vaccine production, we propose that a mechanical stimulation can enhance protein production. In order to prove this hypothesis, Sf9 insect cells were used to evaluate the increase in the expression of a fusion protein from hepatitis B virus (HBV S1/S2). We discovered that the ultrasound stimulation at a frequency of 1.5 MHz, intensity of 60 mW/cm2, for a duration of 10 minutes per day increased HBV S1/S2 by 27%. We further derived a model for transport through a cell membrane under the effect of ultrasound waves, tested the key assumptions of the model through a molecular dynamics simulation package, NAMD (Nanoscale Molecular Dynamics program) and utilized CHARMM force field in a steered molecular dynamics environment. The results show that ultrasound waves can increase cell permeability, which, in turn, can enhance nutrient / waste exchange thus leading to enhanced vaccine production. This finding is very meaningful in either shortening vaccine production time, or increasing the yield of proteins for use as vaccines.

Ultrasound-assisted electrodeposition of SnS: Effect of ultrasound waves on the physical properties of nanostructured SnS thin films

Nanostructured SnS thin films were prepared using an electrochemical deposition method on fluorine doped tin oxide (FTO) substrate from aqueous solution containing 2 mM SnCl2 and 16 mM Na2S2O3. The pH, temperature, time, and deposition potential (E) of the solution were remained at 2.10, 60 °C, 30 minutes and −1 V, respectively. The ultrasonic waves were applied to synthesize ultrasound-assisted electrodeposited SnS thin film. The characterization of deposited thin films was carried out by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), room temperature photoluminescence (PL) and UV–Vis spectroscopy. The deposited SnS nanostructures were polycrystalline with orthorhombic structure. By applying ultrasonic waves, the crystalline quality was decreased. The FESEM images revealed that the ultrasonic waves are responsible for the changes in the morphology of the SnS nanostructures. Also by applying ultrasonic waves, more thin planes were created which led to formation of two dimensional (2D) SnS. The PL spectra of the ultrasound-assisted electrodeposited SnS film showed a red shift compared to the electrodeposited SnS. The optical bandgap energy (Eg) of the ultrasound-assisted electrodeposited SnS decreased from 1.5 eV to 1.45 eV. In addition, the ultrasound-assisted electrodeposited SnS has greater conversion efficiency of solar cell than that of the electrodeposited SnS.

The effect of ultrasound waves on the efficiency of membrane clarification of pomegranate juice

SummaryPomegranate juice has a turbid appearance, which poses difficulties in its concentration process. Membrane clarification can be used to clarify pomegranate juice; however, membrane fouling reduces the permeate flux, limiting its effectiveness. Ultrasound at 30 kHz was used to reduce membrane fouling. Results were compared with the data obtained for membrane clarification without ultrasonic treatment at the same temperature. Results showed that permeate flux increased with ultrasonic treatment. Evaluation of different membrane fouling characteristics showed that the total membrane resistance fell due to the reduction in irreversible fouling and cake resistance. However, ultrasound did not affect the thick caking produced in membrane processing at low feed‐flow rates. Evaluation of the physicochemical properties of pomegranate juice showed that ultrasound can decrease antioxidant activity due to the reduction in total anthocyanin content. Also, total soluble solid content and acidity of pomegranate juice changed with ultrasonic treatment.