Abstract

In this study research was carried out on 5 male adult BALB/c mouse with a weight of 18±5 g. The temperature was at 22±2 °C and animals were kept under the condition of 12 h of light and 12 h of darkness (light on at 08:00 am) and free access to water and standard diet. All 5 mice were affected by three different harmonic oscillations one time and after 24 hours 4 of them were affected by the same oscillations (Fig. 1) and one was placed separately for control. At the end of the experiment, after euthanasia using CO2 chamber blood samples were collected by cardiac puncture. We noticed that ultrasound waves 20 MHz changed mice red blood cells structure and we found pathological forms of erythrocytes. Analysing morphological structure of erythrocytes were determined 46 abnormal red blood structure forms and atypical cells (30 codocytes, 8 stomatocytes and 8 acantocytes). Moreover, we determined that changed some normal behavior characteristics and increased grooming process.

Highlights

  • The use of high-frequency oscillations in the study of vascular pathology has been described in scientific papers [1, 2]

  • Defects in the circuit produce a delay or conduction block and induce cardiac arrhythmia. defects in the ventricular conduction system are a major cause of arrhythmias and deciphering the molecular pathways responsible for conduction system morphogenesis and the differentiation of conductive myocytes furthers our understanding of the mechanisms underlying heart disease [3]

  • Red blood cells morphology were analysed from the prepared slides which were stained with reagent

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Summary

Introduction

The use of high-frequency oscillations in the study of vascular pathology has been described in scientific papers [1, 2]. Ultrasound imaging can replace more invasive techniques that cause pain and distress [3]. Ultrasound is acoustic (sound) energy in the form of waves having a frequency above 20 thousand cycles per second (20.000 Hz). Exposure of ultra wave sounds for a long period of time may influence heart and/or liver function or structure and/or other tissues [5, 6]. The ventricular conduction system represents the electrical wiring responsible for the coordination of cardiac contraction. Defects in the circuit produce a delay or conduction block and induce cardiac arrhythmia. Defects in the ventricular conduction system are a major cause of arrhythmias and deciphering the molecular pathways responsible for conduction system morphogenesis and the differentiation of conductive myocytes furthers our understanding of the mechanisms underlying heart disease [3] Defects in the circuit produce a delay or conduction block and induce cardiac arrhythmia. defects in the ventricular conduction system are a major cause of arrhythmias and deciphering the molecular pathways responsible for conduction system morphogenesis and the differentiation of conductive myocytes furthers our understanding of the mechanisms underlying heart disease [3]

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