Al Mokhtar MU, Ariningrum D, Kustiwinarni. 2009. Effect of tomato juice (Lycopersicum esculentum) on LDL cholesterol level of white rat. Biofarmasi 7: 22-30. The prevalence of Coronary Heart Disease (CHD) over the world is very high. Atherosclerosis due to hypercholesterolemia is a predisposing factor of CHD. The role of high dietary of tomato juice is associated with a lowered LDLcholesterol. Lycopene and naringenin of tomato juice will decrease LDL cholesterol by inhibiting HMG-CoA reductase and upregulating LDL cholesterol receptor in liver. The study aimed to determine the effect of tomato juice on LDL cholesterol in white rat, Rattus norvegicus. The study was an experimental laboratory with pre and post-test controlled group design. Thirty-four adult male Wistar rats, 3 months in age and weighing 180-200 grams, were used in the research. They were divided into two groups, group 1 (KI) as control and group 2 (KII) as treatment group of tomato juice, with 17 rats for each group. They were allowed with standard diet for 7 days. During pretest period for 2 weeks, KI and KII were given hypercholesterolemic fed 100 g/BW ad libitum (mixed diet which consisted of standard diet 88.5%, coconut oil 1%, lard oil 10% and 0.5% crystalline cholesterol), egg yolk force-fed by gastric tube 7.5 ml/BW and PTU 0.1% ad libitum. During post-test period for 2 weeks, KI was given the same intervention with pretest period and aquadest force-fed 30 ml/BW as placebo by gastric tube, whereas KII was given the same intervention with KI and tomato juice forcefed 30 ml/BW by gastric tube. The measurement of the level of LDL cholesterol was conducted by using spectrophotometer. Statistical analysis was performed with SPSS Windows 13. Student’s t-paired analysis was used for variable that normally distributed. Wilcoxon test was used for variable that skewed. Both statistical analyses were used to determine significant differences, the values of p<0.05 were considered statistically significant. The effect of tomato juice by 30 ml/BW on LDL cholesterol for 2 weeks in Rattus norvegicus was determined. The result of t-paired test obtained showed a significant difference (p<0.05) of LDL cholesterol level before and after treatment on KII. Based on the study, there was an effect of tomato juice 30 ml/BW on LDL cholesterol for 2 weeks in Rattus norvegicus.
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