Effect Of Static Magnetic Field Research Articles

Static magnetic field modulates olfactory ensheathing cell's morphology, division, and migration activities, a biophysical approach to regeneration.

The moderate static magnetic fields (SMFs) have been used here as a non-invasive tool to study their manipulative effects on the olfactory ensheathing cells (OECs) activity, growth, morphology, and migration in culture. The OECs are involved in the regeneration of primary olfactory sensory neurons and migration into the central nervous system to repair axons damaged by infection, injury, etc., that play a pivotal role in complementary regenerative medicine. Here, OECs were isolated from the olfactory bulb and cultured to confluence. An in vitro wound healing model was formed and exposed to either parallel (PaSMF) or perpendicular (PeSMF) SMF at intensities of 30, 50, and 70mT, and cells' morphology, podia formation, proliferation, and migration were studied by time-lapse recording. The SMFs were not cytotoxic at the intensity and exposure time applied here. The exposure of cells to 70mT PaSMF and PeSMF increased the formation of lamellipodia and filopodia, cell migration speed, and direction of the scratch forefront cells, significantly. Treatment of cells with 70mT PaSMF and PeSMF increased cell divisions, while 30mT PaSMF decreased it. SMF effects on OECs division, motility, migratory direction, and velocity indicate its effect on various aspects of cell physiology and signaling at atomic and molecular levels, and have a role in tissue regeneration that involves microtubules and actin filaments formation and rearrangements. Thus, the exposure of OECs with moderate SMF might be considered a promising noninvasive approach to remotely manipulate normal and stem cell activities for therapeutic regenerative purposes in various tissues including the central nervous system.

Phase amplitude coupling analysis of local field potentials in working memory of rats affected by transcranial magneto-acoustic-electrical stimulation

Transcranial magneto-acoustic-electrical stimulation is a new non-invasive neuromodulation technology, in which the induced electric field generated by the coupling effect of ultrasound and static magnetic field are used to regulate the neural rhythm oscillation activity in the corresponding brain region. The purpose of this paper is to investigate the effects of transcranial magneto-acoustic-electrical stimulation on the information transfer and communication in neuronal clusters during memory. In the experiment, twenty healthy adult Wistar rats were randomly divided into a control group (five rats) and stimulation groups (fifteen rats). Transcranial magneto-acoustic-electrical stimulation of 0.05~0.15 T and 2.66~13.33 W/cm 2 was applied to the rats in stimulation groups, and no stimulation was applied to the rats in the control group. The local field potentials signals in the prefrontal cortex of rats during the T-maze working memory tasks were acquired. Then the coupling differences between delta rhythm phase, theta rhythm phase and gamma rhythm amplitude of rats in different parameter stimulation groups and control group were compared. The experimental results showed that the coupling intensity of delta and gamma rhythm in stimulation groups was significantly lower than that in the control group ( P<0.05), while the coupling intensity of theta and gamma rhythm was significantly higher than that in the control group ( P<0.05). With the increase of stimulation parameters, the degree of coupling between delta and gamma rhythm showed a decreasing trend, while the degree of coupling between theta and gamma rhythm tended to increase. The preliminary results of this paper indicated that transcranial magneto-acoustic-electrical stimulation inhibited delta rhythmic neuronal activity and enhanced the oscillation of theta and gamma rhythm in the prefrontal cortex, thus promoted the exchange and transmission of information between neuronal clusters in different spatial scales. This lays the foundation for further exploring the mechanism of transcranial magneto-acoustic-electrical stimulation in regulating brain memory function.

Field-dependent shape and magnetic spectrum of magnetic bimeron

Magnetic bimeron composed of a meron and an antimeron can be considered as a magnetic skyrmion in the in-plane magnet and have great potential for spintronic devices. Here, we explored the effect of static magnetic fields on the shape of bimeron. It is found that the size of the bimeron can be adjusted by magnetic fields in an anisotropic direction. Then we study the field-tuned spin excitation spectrum of magnetic bimeron. Unlike the common breathing mode and the two rotation modes, novel resonance modes, which can be considered as a combination of the breathing and rocking-rotation modes, are observed. The results presented in this study can help to open novel research into the spintronics of magnetic bimeron.

Effects of Magnetite Nanoparticles and Static Magnetic Field on Neural Differentiation of Pluripotent Stem Cells.

Neurodevelopmental processes of pluripotent cells, such as proliferation and differentiation, are influenced by external natural forces. Despite the presence of biogenic magnetite nanoparticles in the central nervous system and constant exposure to the Earth's magnetic fields and other sources, thereisscant knowledge regarding the role of electromagnetic stimuli in neurogenesis. Moreover, emerging applications of electrical and magnetic stimulation to treat neurological disorders emphasize the relevance of understanding the impact and mechanisms behind these stimuli. Here, the effects of magnetic nanoparticles (MNPs)in polymeric coatings and the static external magnetic field (EMF) were investigated on neural induction of murine embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs). The resultsshowthat the presence of 0.5% MNPs in collagen-based coatings facilitates the migration andneuronal maturationof mESCs and hiPSCs in vitro. Furthermore, theapplication of 0.4 TeslaEMF perpendicularly to the cell culture plane, discernibly stimulates proliferationand guide fate decisions of thepluripotent stem cells, depending on the origin of stem cells and their developmental stage. Mechanistic analysis revealsthat modulation of ionic homeostasis and theexpression of proteins involved in cytostructural,liposomaland cell cycle checkpoint functions provide a principal underpinning for the impact of electromagnetic stimuli on neural lineage specification andproliferation. These findings not only explore the potential of the magnetic stimuli as neural differentiation and functionmodulator but also highlight the risks that immoderate magnetic stimulation may affect more susceptible neurons, such as dopaminergic neurons.

Distinct fatty acid redistribution and textural changes in the brain tissue upon the static magnetic field exposure

We observed different outcomes upon the subacute exposure to the 128 mT highly homogeneous static magnetic field (SMF) when its orientation was (i) aligned with the vertical component of the geomagnetic field; (ii) in the opposite direction. We employed the fatty acids (FA) composition and digital image analyses (DIA) to provide insights into the underlying processes and examine the possible weak SMF effects. Swiss-Webster male mice were whole-body exposed for 1 h/day over five days. Brain tissue’s thin liquid chromatography resulted in brain FA composition, indicating a possible sequence of changes due to the SMF exposure. Quantitative DIA accurately assessed different image parameters. Delicate textural changes were revealed in the group where pathohistological or biochemical alterations have not been detected. DIA-based biological markers seem to be very promising for studying delicate tissue changes, which results from the high sensitivity and wide availability of DIA.

Static Magnetic Field Inhibits Growth of Escherichia coli Colonies via Restriction of Carbon Source Utilization.

Magnetobiological effects on growth and virulence have been widely reported in Escherichia coli (E. coli). However, published results are quite varied and sometimes conflicting because the underlying mechanism remains unknown. Here, we reported that the application of 250 mT static magnetic field (SMF) significantly reduces the diameter of E. coli colony-forming units (CFUs) but has no impact on the number of CFUs. Transcriptomic analysis revealed that the inhibitory effect of SMF is attributed to differentially expressed genes (DEGs) primarily involved in carbon source utilization. Consistently, the addition of glycolate or glyoxylate to the culture media successfully restores the bacterial phenotype in SMF, and knockout mutants lacking glycolate oxidase are no longer sensitive to SMF. These results suggest that SMF treatment results in a decrease in glycolate oxidase activity. In addition, metabolomic assay showed that long-chain fatty acids (LCFA) accumulate while phosphatidylglycerol and middle-chain fatty acids decrease in the SMF-treated bacteria, suggesting that SMF inhibits LCFA degradation. Based on the published evidence together with ours derived from this study, we propose a model showing that free radicals generated by LCFA degradation are the primary target of SMF action, which triggers the bacterial oxidative stress response and ultimately leads to growth inhibition.

Effect of static magnetic field treatment on the germination of brown rice: Changes in α-amylase activity and structural and functional properties in starch

The study aimed to explore the stimulating effect of static magnetic field (SMF) treatment on germinated brown rice (GBR) by monitoring changes in α-amylase activity and structural and functional properties of starch. Brown rice was exposed to SMF (10 mT, 60 min, 25 °C) and then germinated for 0 h −72 h at 30 °C. Compared with the control, SMF treatment improved α-amylase activity (15.2%), leading to the hydrolysis of starch into reducing sugar (8.2%) and increasing the germination rate (9.7% −158.8%), shoot length (9.1% −87.3%), root length (19.2% −110.0%), and fresh weight (0.9% −16.5%). In view of the properties of starch, SMF treatment also altered the surface microstructure, induced partial losses of birefringence, exerted no significant effect on the crystalline type, slightly increased the gelatinization temperatures, and significantly decreased the peak viscosity. This study suggested that SMF could serve as a prospective technique for GBR products processing.

Formation mechanism of ring-like segregation and structure during directional solidification under axial static magnetic field

• The ring-like structure forms under a moderate axial magnetic field. • The ring-like convection and segregation form under a moderate axial magnetic field. • The formation of the ring-like structure is attributed to solute redistribution under magnetic field. The effect of the axial static magnetic field on the macro-segregation and structure in the Al-Cu and Ni-Mn-Ga alloys during directional solidification is investigated experimentally and numerically. It is found that the ring-like segregation and structure in the above-mentioned two alloys form during directional solidification at a certain growth speed under a moderate magnetic field. For the Al-Cu and Ni-Mn-Ga alloys, the moderate values of the magnetic field under which the ring-like structure forms are about 0.5 T and 1.0 T at respective growth speed of 10 μm/s and 5 μm/s. Further, the distributions of the flow and solute in the Al-Cu alloy during directional solidification under the axial static magnetic field is numerically simulated. Numerical results reveal that the rotary thermoelectric (TE) magnetic convection forms in the mushy zone during directional solidification under an axial magnetic field. This flow will induce the formation of the ring-like macro-segregation and structure. Changes in structures under the magnetic field in the experimental results are in good agreement with the distributions of the TE magnetic convection and solute in the numerical results. Therefore, the formation of the ring-like structure and segregation under the magnetic field should be attributed to the solute redistribution induced by the TE magnetic convection.

Moderate intensity of static magnetic fields can alter the avoidance behavior and fat storage of Caenorhabditis elegans via serotonin.

Man-made static magnetic fields (SMFs) widely exist in human life as a physical environmental factor.However, the biological responses to moderate SMFs exposure and their underlying mechanisms are largely unknown. The present study was focused on exploring the nervous responses to moderate-intensity SMFs at 0.5T and 1T in Caenorhabditis elegans (C. elegans). We found that SMFs at either 0.5T or 1T had no statistically significant effects on the locomotor behaviors, while the 1T magnetic field increased pharyngeal pumping. The avoidance behavior of the pathogenic Pseudomonas aeruginosa was greatly decreased in either 0.5T or 1T SMFs exposed nematodes, and the learning index was reducede from 0.52 ± 0.11 to 0.23 ± 0.17 and 0.16 ± 0.11, respectively. The total serotonin level was increased by 17.08% and 16.45% with the treatment of 0.5T and 1T SMF, compared to the control group; however, there were minimal effects of SMFs on other three neurotransmitters including choline, γ-aminobutyric acid (GABA), dopamine. RT-qPCR was used to further investigate the expression of serotonin-related genes, including rate-limiting enzymes, transcription factors and transport receptors. The expression levels of tph-1 and unc-86 genes were increased by SMF exposure, while those of ocr-2, osm-9, ser-1 and mod-1 genes were decreased. With the staining of lipid in either wild-type N2 or tph-1 mutants, we found that 0.5T and 1T SMFs decreased fat storage in C. elegans via serotonin pathway. Our study demonstrated that moderate-intensity SMFs induced neurobehavioral disorder and the reduction of fat storage by disturbing the secretion of serotonin in C. elegans, which provided new insights into elucidating nervous responses of C. elegans to moderate-intensity SMFs.

Uticaj stalnog magnetnog polja na sadržaj minerala kostiju starih pacova

Introduction: Aging is defined as a consequence of progressive accumulation of metabolic waste, which results in development of various disorders in the structure and function of cells over a number of years. It is followed by loss of bone tissue, where bones become less firm. The cycle of bone remodeling with age becomes longer, and the degree of bone mineralization decreases. Static magnetic fields (SMF) are stable magnetic fields that can be natural or artificial. Their moderate intensity (1mT-1T) affects physiological processes, cells, genetic material, behavior and development. So far, numerous studies have shown different effects of static magnetic fields on cell cultures, experimental animals, and the human population. Aim: Aim of this study was to determine the effect of static magnetic field (SMF) on bone mineral content (BMC) values in aging rats. Material and methods: Male Wistar rats, 3 years old, were used in the experiment. A total of 18 animals were divided into two groups: exposed (experimental group) and unexposed (control group). Nine animals from the experimental group were exposed to 30mT intensity static magnetic field for 10 weeks, while nine control rats were not exposed to the static magnetic field. Bone mineral content was measured by DXA (Dual-Energy X-Ray Absorptiometry. Results: Based on the experiment, it was found that after the exposure to the static magnetic field, in the experimental group, there is a statistically significant increase in the value of BMC in the trunk region and ribs region. In all other regions of interest: head, legs, pelvic bone, spine, total BMC - there were no statistically significant changes in BMC values. Conclusion: In the conducted experiment, a higher increase in the value of BMC was found in animals exposed to the SMF, compared to animals that were not exposed to the static magnetic field.

Effects of static magnetic fields on the cardiac implantable devices and their comparison

When placed in an environment with the magnetic flux density higher than a certain value, cardiac implantable electronic devices (CIEDs) possibly show magnet response and affect patients’ safety.

Effect of Static Magnetic Field on Hydrogen and N-Caproic Acid Production by Dark Fermentation with No Pretreatment Excess Sludge as Inoculum

Effect of Static Magnetic Field on Hydrogen and N-Caproic Acid Production by Dark Fermentation with No Pretreatment Excess Sludge as Inoculum

Effects of Online Static Magnetic Field on Anisotropy of Microstructure and Mechanical Properties of GH3536 Fabricated by Selective Laser Melting

Effects of Online Static Magnetic Field on Anisotropy of Microstructure and Mechanical Properties of GH3536 Fabricated by Selective Laser Melting

Magnesium–Magnetic Field Synergy Enhances Mouse Bone Marrow Mesenchymal Stem Cell Differentiation into Osteoblasts Via the MAGT1 Channel

Magnesium ion (Mg2+)‐based materials are known to exert osteogenic effects that can be enhanced by the bioelectrical properties of magnetic fields. In this study, we examined the effect of a medium‐strength static magnetic field (SMF), combined with a Mg2+‐containing medium, on the proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells (BMSCs). Mouse BMSCs were divided into a control group, 7.5 mM Mg2+ group, 15 mT SMF group, and 7.5 mM Mg2+ plus 15 mT SMF group. Osteoblast proliferation was measured using a Cell Counting Kit‐8 assay, whereas osteogenic differentiation was detected using alkaline phosphatase (ALP) staining and western blot analysis, respectively. The number and size of calcium nodules were determined using Alizarin Red staining. Compared with those in the control group, the ALP activity, calcium nodule formation, and osteogenic protein expression were promoted in other groups. In particular, Mg2+‐SMF had a significant effect after 7 days of intervention and more effectively promoted BMSC differentiation and proliferation than either Mg2+ or the SMF alone, suggesting that Mg2+‐SMF synergistically contributed to osteogenic differentiation and cell proliferation. To examine their roles in bone differentiation, the Magt1 and Creb1 genes were silenced in BMSCs, and the findings indicated that the synergistic intervention with Mg2+ and magnetic fields might exert osteogenic effects via the MAGT1 channel and CREB1 protein. This study provides an experimental basis for a potential Mg2+‐SMF synergistic artificial bone material that could be clinically applied in the treatment of bone defects.

Воздействие магнитных полей различной интенсивности и синтетических олигопептидов на клеточную регенерацию тканей

The Earth’s magnetic field is subject to continuous changes. It has also been shown to induce effect on the vital activities of all living organisms. These factors make the study of magneto-biological effects important and highly relevant. From the biological point of view, weak magnetic fields, especially weak static magnetic fields, are among the most poorly understood, however, they have a noticeable effect on living organisms, including humans. The use of such fields is on the rise, which requires a comprehensive understanding of mechanisms behind their effect on living things. The article investigates the effect of weak static magnetic fields amplified and weakened relative to the Earth’s magnetic field on cellular tissue regeneration. It has been shown that one of the main cellular processes—proliferation—increases when the cells are exposed to enhanced or depressed static magnetic fields. The greatest effect of such exposure is observed in mesodermal tissue, i. e., the myocardium, vessels, and muscles. The effect of tissue-specific oligopeptides on cellular proliferation is comparable to that of static magnetic fields: the stimulation of cellular regeneration occurs primarily in the myocardium, muscles, and vessels. A special focus is given to the therapeutic potential of weak magnetic fields and their interaction with clinical drugs in various pathologies.

Effects of static magnetic fields on onion (Allium cepa L.) seed germination and early seedling growth

In vegetables of economic importance such as onion, one of the main limitations in their production is that their seeds have a relatively short storage life, so their viability decreases rapidly. Research has been carried out on onions to improve seed germination and to extend its use for sowing. The magnetic field is considered a simple, inexpensive, and non-invasive physical method to stimulate the germination process, compared to traditional chemical methods. In this sense the objective of this research were to evaluate the effects of static magnetic fields on Yellow Granex PRR hybrid onion (Allium cepa L.) seed germination, and early growth in the laboratory conditions. Seeds were exposed to 10 and 21mT, (mT=militesla), static magnetic fields induced by magnets for 0, 5, 3, 6, 12 and 24h; each treatment had four repetitions. The results showed that the low intensity stationary magnetic fields (10 and 21mT) did not cause significant differences in germination, dry weight, or fresh weight, but for the seedling length. It is necessary to increase the intensity of the magnetic fields and the exposure time to achieve important physiological changes that positively affect the germination and growth of onion seeds, and thus contribute to the improvement of their yield and productivity. The use of physical methods such as magnetism can stimulate different physiological processes in plants and thus contribute to the improvement of characteristics of agronomic interest.

Effects of static magnetic fields on onion (Allium cepa L.) seed germination and early seedling growth

In vegetables of economic importance such as onion, one of the main limitations in their production is that their seeds have a relatively short storage life, so their viability decreases rapidly. Research has been carried out on onions to improve seed germination and to extend its use for sowing. The magnetic field is considered a simple, inexpensive, and non-invasive physical method to stimulate the germination process, compared to traditional chemical methods. In this sense the objective of this research were to evaluate the effects of static magnetic fields on Yellow Granex PRR hybrid onion (Allium cepa L.) seed germination, and early growth in the laboratory conditions. Seeds were exposed to 10 and 21mT, (mT=militesla), static magnetic fields induced by magnets for 0, 5, 3, 6, 12 and 24h; each treatment had four repetitions. The results showed that the low intensity stationary magnetic fields (10 and 21mT) did not cause significant differences in germination, dry weight, or fresh weight, but for the seedling length. It is necessary to increase the intensity of the magnetic fields and the exposure time to achieve important physiological changes that positively affect the germination and growth of onion seeds, and thus contribute to the improvement of their yield and productivity. The use of physical methods such as magnetism can stimulate different physiological processes in plants and thus contribute to the improvement of characteristics of agronomic interest.

Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells.

There are numerous studies that investigate the effects of static magnetic fields (SMFs) on osteoblasts and osteoclasts. However, although osteocytes are the most abundant cell type in bone tissue, there are few studies on the biological effects of osteocytes under magnetic fields. Iron is a necessary microelement that is involved in numerous life activities in cells. Studies have shown that high static magnetic fields (HiSMF) can regulate cellular iron metabolism. To illustrate the effect of HiSMF on activities of osteocytes, and whether iron is involved in this process, HiSMF of 16 tesla (T) was used, and the changes in cellular morphology, cytoskeleton, function-related protein expression, secretion of various cytokines, and iron metabolism in osteocytes under HiSMF were studied. In addition, the biological effects of HiSMF combined with iron preparation and iron chelator on osteocytes were also investigated. The results showed that HiSMF promoted cellular viability, decreased apoptosis, increased the fractal dimension of the cytoskeleton, altered the secretion of cytokines, and increased iron levels in osteocytes. Moreover, it was found that the biological effects of osteocytes under HiSMF are attenuated or enhanced by treatment with a certain concentration of iron. These data suggest that HiSMF-regulated cellular iron metabolism may be involved in altering the biological effects of osteocytes under HiSMF exposure.

Revealing the effects of static magnetic field on the anoxic/oxic sequencing batch reactor from the perspective of electron transport and microbial community shifts

The effects of static magnetic field (SMF) on an anoxic/oxic sequencing batch reactor were investigated from the perspective of electron transport via determining the variations of reduced/oxidized nicotinamide adenine dinucleotide (NADH/NAD+) ratio, NADH concentration, electron transport system activity (ETSA), poly-β-hydroxybutyrate (PHB), extracellular polymeric substances (EPS), as well as the gene expression under different conditions. Moreover, the shifts of microbial community were also analyzed. The application of SMF with an appropriate intensity significantly improved the performance of the process, the abundance of the anoxic denitrifiers, and the activity of the aerobic denitrifiers. The NADH content, as well as ETSA were also enhanced, therefore, the total nitrogen removal efficiency of the process was increased. However, the overhigh SMF intensity resulted in the change of microbial community, meanwhile, had negative effects on the metabolism of microorganisms. Selecting a proper intensity is crucial for the SMF-enhanced biological wastewater treatment process.

Static Magnetic Fields Enhance the Chondrogenesis of Mandibular Bone Marrow Mesenchymal Stem Cells in Coculture Systems.

Objectives Combining the advantages of static magnetic fields (SMF) and coculture systems, we investigated the effect of moderate-intensity SMF on the chondrogenesis and proliferation of mandibular bone marrow mesenchymal stem cells (MBMSCs) in the MBMSC/mandibular condylar chondrocyte (MCC) coculture system. The main aim of the present study was to provide an experimental basis for obtaining better cartilage tissue engineering seed cells for the effective repair of condylar cartilage defects in clinical practice. Methods MBMSCs and MCCs were isolated from SD (Sprague Dawley) rats. Flow cytometry, three-lineage differentiation, colony-forming assays, immunocytochemistry, and toluidine blue staining were used for the identification of MBMSCs and MCCs. MBMSCs and MCCs were seeded into the lower and upper Transwell chambers, respectively, at a ratio of 1 : 2, and exposed to a 280 mT SMF. MBMSCs were harvested after 3, 7, or 14 days for analysis. CCK-8 was used to detect cell proliferation, Alcian blue staining was utilized to evaluate glycosaminoglycan (GAG), and western blotting and real-time quantitative polymerase chain reaction (RT-qPCR) detected protein and gene expression levels of SOX9, Col2A1 (Collagen Type II Alpha 1), and Aggrecan (ACAN). Results The proliferation of MBMSCs was significantly enhanced in the experimental group with MBMSCs cocultured with MCCs under SMF stimulation relative to controls (P < 0.05). GAG content was increased, and SOX9, Col2A1, and ACAN were also increased at the mRNA and protein levels (P < 0.05). Conclusions Moderate-intensity SMF improved the chondrogenesis and proliferation of MBMSCs in the coculture system, and it might be a promising approach to repair condylar cartilage defects in the clinical setting.