Effect Of Short-term Storage Research Articles

Effect of short-term storage on cannabinoid content of dried floral hemp (Cannabis sativa L) inflorescence

Floral hemp, a plant rich in cannabinoids, has gained attention for its potential therapeutic properties. It is crucial to have proper storage conditions that will maintain the cannabinoid content. To the best of our knowledge, very little has been done on the storage conditions of dried floral hemp. Hence, this study investigated the effect of storage conditions like moisture content, temperature, storage time, light exposure, and packaging materials, on cannabinoid stability in dried floral hemp. The cultivar ‘Quick Spectrum’ at two moisture levels (8.5 and 4.5 %) was stored over a 6-month storage period. Results indicated that a higher amount of cannabinoids samples was retained at 8.5 ± 1 % moisture compared to samples at 4.5 ± 0.5 %, even though total CBD, total ∆9-THC, and total CBDV content remained statistically the same throughout the storage period, and was not affected by temperature, lighting, and packaging material. In contrast, certain individual cannabinoids, such as CBDVA, CBD, ∆9-THCA, and ∆9-THC were significantly affected by temperature and lighting. Notably, higher storage temperatures (22 °C) and light exposure (F15T8/F5 fluorescent light) increased the degradation and decarboxylation rate of cannabinoids. These findings showed the importance of optimized storage conditions for floral hemp, especially long-term storage. This will help provide standardized storage protocols to aid producers, testing laboratories, distributors, and consumers in making informed decisions about storage practices and advancing quality control in the hemp industry.

Time of Consolidation and Humidity Influence on Properties of Food Powders

The effect of short-term storage at 75% and 90% ambient humidity on the mechanical properties of selected materials was determined using a new device for measuring the strength of food powders. A series of tests were conducted on wheat flour and potato starch subjected to various consolidation loads. The high accuracy and repeatability of the measurements confirmed the suitability of the pull-based tester for assessing the degree of caking in food powders. The pull-based tester allows for the measurement of strength parameters of agglomerates under various consolidation loads while simultaneously wetting the powder, introducing a novel approach to assessing the mechanical properties of powders. The analysis of force oscillation during the withdrawal of the measuring rod from the powder facilitates the identification of the slip-stick effect in these materials and the determination of parameters characterizing that phenomenon. The outcomes of this study may be of interest to farmers, manufacturers, and companies processing raw materials.

Effect of Short-term Storage in a High-humidity Refrigerator on the Quality of Asparagus (<i>Asparagus officinalis</i> L.) during Storage and Display

アスパラガス（Asparagus officinalis L.）は収穫後の代謝活性が非常に高く，品質を維持するには低温条件で貯蔵することが非常に重要である．本研究では，通常の冷蔵庫では困難な低温かつ高湿度条件を作り出す高湿度冷蔵庫による貯蔵がアスパラガスの若茎の重量，伸長および品質に及ぼす影響を調査した．高湿度冷蔵庫と冷蔵庫でアスパラガスを貯蔵し，経時的にそれらの重量と長さを調査した結果，貯蔵11時間以降，冷蔵庫区より高湿度冷蔵庫区で，若茎の重量と長さの減少量は有意に小さくなり，高湿度冷蔵庫が貯蔵中の若茎の重量減少抑制に有効であることが明らかとなった．次に，各試験区における貯蔵が販売所での陳列・棚持ち期間中の若茎の品質に及ぼす影響を明らかにするため，各冷蔵方式により0～72時間貯蔵した若茎を冷蔵ショーケースで3日間水につけて保管し，保管後の若茎の品質を調査した．その結果，両試験区間に大きな差は認められなかったものの，高湿度冷蔵庫での貯蔵は若茎の急激な重量の増減を抑制したことから，収穫直後の効果的な短期冷蔵技術であると考えられた．また，高湿度区では貯蔵時間が長くなるにつれ，保管後の若茎の重量と伸長量の増加量が小さくなり，糖度が高くなることが判明した．これは低温下での貯蔵が若茎の代謝活性を抑制し，それが若茎の伸長を抑制したことに起因すると考えられた．本研究の結果から，アスパラガスの貯蔵における高湿度冷蔵庫の有用性が示されるとともに，低温下での貯蔵時間が保管後の若茎の品質に影響を与える可能性が示された．この成果はアスパラガスの貯蔵・出荷の体系を考える上での有用な知見となると考えられる．

Longevity and germination of Juniperus communis L. pollen after storage

Pollen storage belongs among the most important activities associated with pollen handling. It overcomes the differences in pollen shedding and ovule receptivity during controlled pollination experiments. It is especially important for species like common juniper (Juniperus communis L.) with an extremely low quality of seeds due to pollination failure. Additionally, it is a substantial part of germplasm preservation programmes in pollen banks. In the present paper, the effect of short-term storage of pollen was studied using pollen samples from five shrubs in an in vitro germination test. Two temperature regimes were tested. The pollen viability of freshly collected pollen varied considerably between individual shrubs, exhibiting 67.3–88.6% germination rate and 248.0–367.3 µm of pollen tubes. Storage at + 4 °C for four months was accompanied by a profound decline in pollen viability. The germination percentage was reduced to 49.2–75.2% and the pollen tube length to 32.5–69.0%, depending on individual shrubs. The corresponding decline in pollen viability characteristics during storage at − 20 °C was only negligible in two of the tested shrubs. In the remaining three shrub samples, an increase in germination percentage was observed. Pollen tube growth responded more sensitively to freezing, but, on average, the decrease in length was lower than that at + 4 °C. The rate of reduction in pollen tube length varied between 11.5 and 45.4%. Cytological events accompanying in vitro germination of freezer-stored pollen exhibited some delay in releasing the exine from pollen grains during the early stages of germination as compared with freshly collected pollen. In conclusion, short-term storage of the common juniper pollen in a freezer is better for the preservation of its viability than storage at + 4 °C.

Effect of short term storage on wheat quality parameters

Eleven samples of registered wheat varieties of bread with diverse technological qualities were used in this study. The samples were devided into two groups. The first group including all the 11 variety were stored for 3 months, while the second group of the samples were stored for 9 months at an ambient temperature. The results of quality evaluation showed that 5 soft wheat varieties (GK Csongrád, GK Garaboly, GK Hattyú, GK Holló, GK Nap) and 6 hard wheat varieties (GK Ati, GK Békés, GK Élet, GK Kalász, GK Petúr, GK Verecke) were involved in the study. Further, the flour yield, the gluten index and the water absorbance capacity has significantly decreased after 9 months storage time when compared to 3 months storage interval.

Effects of Short-Term Storage or Pre-Storage Heating of Hatching Eggs from Young Broiler Breeder Flock before Incubation on Hatching Parameters and Chick Quality

Effects of Short-Term Storage or Pre-Storage Heating of Hatching Eggs from Young Broiler Breeder Flock before Incubation on Hatching Parameters and Chick Quality Canan KOP-BOZBAY Abstract This study was conducted to evaluate the effects of short-term storage (four days) or pre-storage heating of hatching eggs from young Ross broiler breeding flocks (28 week old) on hatching parameters and chick quality. In total, 1350 hatching eggs of 1 d and 4 d of laid were individually numbered and distributed three treatments, named as NS (eggs that were not stored), S (eggs that were stored for four days before incubation) and HS (eggs that were heated at 37.7 °C and a relative humidity of 60% for 24 h and then stored for three days before incubation). The HS treatment increased the hatchabilities of set and fertil eggs compared to the NS and S treatments, whereas the S decreased the these variables compared to the NS treatment. The embrionic mortality in the HS eggs was lower than those in the NS and S eggs. The rate of discarded chicks was higher in the S group than in the HS group. The chick weight in the NS treatment was higher than those in the S and HS treatments. In conclusion, the pre-storage heating of hatching eggs from young broiler breeder flock positively affected hatchability without chick weight and quality. Full Text: PDF DOI: 10.15640/jaes.v8n2a14

Retraction of articles by Dr M. Aramli.

The below articles published online on Wiley Online Library (wileyonlinelibrary.com) have been retracted by agreement between the submitting author, Mohammad Sadegh Aramli, the Editor-in-Chief, Heriberto Rodriguez-Martinez, and Blackwell Verlag GmbH. After a thorough investigation, there is strong evidence to indicate that the peer review of these papers was compromised. The identities of the reviewers were unable to be verified, and it is believed that these papers were accepted based on recommendations from reviewers not suitably qualified. REFERENCES Aramli, M. (2014). ATP content, oxidative stress and motility of beluga (Huso huso) semen: Effect of short-term storage. Reproduction in Domestic Animals, 49, 636-640. https://doi.org/10.1111/rda.12339 Aramli, M., Nazari, R., & Gharibi, M. (2015), Effect of post-thaw storage time on motility and fertility of cryopreserved beluga sturgeon (Huso huso) sperm. Reproduction in Domestic Animals, 50, 349-352. https://doi.org/10.1111/rda.12484 Aramli, M., Golshahi, K., Nazari, R., Golpour, A., & Aramli, S. (2016). Influence of glutamine supplementation on motility and fertilization success of frozen-thawed persian sturgeon (Acipenser persicus) sperm. Reproduction in Domestic Animals, 51, 474-477. https://doi.org/10.1111/rda.12704 Aramli, M., Golshahi, K., Banan, A., & Sotoudeh, E. (2016). Reliable collection of Caspian brown trout (Salmo trutta caspius) sperm using a catheter. Reproduction in Domestic Animals, 51, 831-834. https://doi.org/10.1111/rda.12740 Aramli, M., Nazari, R., Aramli, S., & Nouri, H. (2017). Motility and oxidative-antioxidant capacity of Huso huso semen, stored at -80°C. Reproduction in Domestic Animals, 52, 170-173. https://doi.org/10.1111/rda.12814.

Reference limits of the urinary gamma-glutamyltransferase in a healthy population and effects of short-term storage on the enzyme activity

BackgroundGamma-glutamyltransferase (GGT) is present mainly in proximal renal tubule, and urinary GGT is an indicator of tubular damage since it may show renal changes before they are identified by using conventional measurements. Therefore, it is of interest to establish the reference limits of urinary GGT for a healthy population, as well as to investigate the stability of GGT in urine samples stored at 4 °C and −20 °C. MethodsGGT was assessed in urine samples from 127 healthy patients by use of a reference method based on the 5-Amino-2-Nitrobenzoate formation. Stability of GGT was evaluated in 10 urine samples stored at temperatures of 4 °C and −20 °C for a period up to 4 weeks. ResultsUrinary GGT values for healthy volunteers were 14 U/g creatinine for the lower reference limit and 79 U/g creatinine for the upper reference limit. Urinary GGT values were approximately 56% lower in samples stored at −20 °C than fresh samples, while samples stored at 4 °C presented a decrease of 11% in GGT values compared to fresh samples. ConclusionsReference limits for urinary GGT in healthy subjects were 14 to 79 U/g creatinine, and it is recommended to measure urinary GGT in fresh specimens.

Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameters in Patagonian blenny. Samples of undiluted (C: control) and diluted semen 1:1v/v (DS1) and 1:3v/v (DS2) in Storfish® were stored at 4°C for 7days. Motility, viability, mitochondrial membrane potential (ΔΨMit), DNA fragmentation and ATP content were assessed at 0, 3 and 7days. The results demonstrated that sperm function parameters were affected significantly by the time of storage, and that dilution allows better preservation of sperm motility. On the third day of storage, spermatozoa showed a motility >56% in DS1 and DS2, nevertheless, in C it significantly decreased as well as its ATP content (P<0.05), whereas viability was maintained >70% and ΔΨMit >45%. However, a significant decline in the percentage of sperm function parameters (P<0.05) was observed at 7days of storage for all samples of undiluted and diluted semen. DNA fragmentation showed no significant increase in all groups, demonstrating <5% at day 7. In vitro storage conditions maintain better sperm motility in diluted than undiluted semen, preserving the sperm function in all groups assessed during 3days. In conclusion, the results provide new data on Patagonian blenny sperm quality with respect to in vitro short-term storage evaluated by flow cytometry, this information must be considered for implementing in aquaculture reproductive strategies for the conservation of this species.

Nutritional value of the kelps Alaria esculenta and Saccharina latissima and effects of short-term storage on biomass quality

Storage of macroalgae in seawater, prior to further processing, is a standard initial pre-treatment step after harvest to avoid rapid degradation of the biomass. In the context of using seaweeds in human food and animal feed products, such practice may affect the nutritional value and the overall quality of the biomass. The effects of seawater storage on the chemical composition (i.e., mineral fraction, carbohydrates, proteins, polyphenols, and fucoxanthin) and surface color of two cultivated kelps (Phaeophyceae), Alaria esculenta and Saccharina latissima, were investigated over a 22-h period. Storage treatments resulted in a rapid decrease in dry weight during the first 2 h (−21.4 and −20.4% in A. esculenta and S. latissima, respectively) with subsequent stabilization. Although it is not clear whether the reduction of dry weight was caused by the release of nutritional compounds from seaweed biomass or water uptake during storage treatment, the results from chemical analyses suggest the combined effect of both mechanisms. Seawater storage increased the ash and sodium contents and reduced carbohydrate and polyphenol levels in both species. Among carbohydrates, the levels of mannitol and glucose (laminaran) were particularly reduced in S. latissima samples while the fucose level, reflecting fucoidans, was reduced in A. esculenta. The protein content remained relatively stable in both species. These results provide evidence of the effect of seawater storage on the quality of the edible kelps A. esculenta and S. latissima. The results will contribute to selecting postharvest strategies adequate for maintaining biomass quality, minimizing losses of valuable compounds and increasing profitability for industrial stakeholders.

The Effect of Short-term Storage and the Hot Water Treatment of Fresh-Cut Pepper Fruit cv. ‛Blondy F1’ and ‛Yecla F1’ on the Content of Bioactive Compounds and Antioxidant Properties

AbstractThe aim of the experiment was to determine the changes in the contents of bioactive compounds and antioxidant/antiradical activity in fresh-cut fruits of sweet pepper cv. ‘Blondy F1’ and ‘Yecla F1’, depending on the postharvest treatment. Treatment with hot water and short-term storage decreased the content of bioactive compounds and antioxidant/antiradical activity of fruit peppers. The treatment with water at 55 °C for 12 sec. of fresh-cut fruits caused lower losses in content of bioactive compounds and antioxidant/antiradical activities than the treatment with water at 45 °C for 10 min. There was a significant correlation between the content of total polyphenols and antioxidant and antiradical activity of pepper fruits depending on the cultivar and the experimental season.

Effect of short-term storage on walnut fruit quality

Two Hungarian and two introduced walnut cultivars were examined as fresh samples and under storage. Some important compositional data were determined in order to evaluate the differences between the cultivars and the changes after storage. The cultivars’ oil contents were the same, but there was some difference in fatty acid composition; ‘Alsószentiváni 117’ had a higher mono-unsaturated fatty acid content than the others. Considering the oxidative stability, ‘Alsószentiváni 117’ contained good properties in all stages (induction time equals or higher than 10 h). Lower antioxidant capacity was measured in ‘Chandler’ samples compared to the others. ‘Alsószentiváni 117’ has higher α- and γ-tocopherol contents than the other cultivars studied. Two different drying methods were also compared in case of the Hungarian cultivars. The changes in compositional data showed no tendencies. We found that Hungarian cultivars were at least as valuable as the introduced ones.

Stability of an extemporaneously prepared bleomycin–lidocaine–epinephrine intradermal admixture used in dermatology

Bleomycin (0.75 g/L)-lidocaine (3.5 g/L)-epinephrine (3.5 mg/L) admixture (BLE) is indicated for keloids treatment. Effect of short-term storage, influence of BLE components and temperature on efficacy and chemical compatibility of bleomycin were studied. The stability study of BLE was conducted in polypropylene infusion bags at 4℃ and 22℃ over a period of 7 days versus bleomycin diluted alone in normal saline and stored in the same conditions. Active Bleomycin fractions, bleomycin A2 (BA2) and bleomycin B2 (BB2) and inactive demethylbleomycin A2 (DBA2) were analyzed by a validated stability-indicating HPLC method. BA2 and BB2 remained stable for up to 7 days, in both types of bleomycin preparations, whatever the temperature (<10% loss of the initial peak response). In BLE, DBA2 fraction (2.2% at T0) decreased with time. Less degradation was noted with refrigeration (remained % at day 7: 0.86 ± 0.06% at 4℃ versus 0.43 ± 0.02% at 22℃; p = 0.0004). A degradation product (DP) was observed. More significant percentage (of the total of bleomycin fractions) was reached at room temperature (at day 7, 0.97 ± 0.03% at 4℃ versus 1.41 ± 0.06% at 22℃, p = 0.0004). DP attained an identification threshold of 0.5% between the third and fourth days at 4℃ and between the first and the second days at 22℃. A maximum of three days storage at 4℃ and only one day at 22℃ was approved for BLE.

The effect of short term storage on different winter wheat varieties rheological properties

The aim of storage after harvest is to protect the quality of wheat, because after-ripening occurs in the first 5–6 weeks. During this time it very important to make the optimal storage conditions.&#x0D; We have carried out storage experiment with wheat samples from Látókép Research Farm of the University of Debrecen. We analyzed the rheological parameters of Lupus and GK Csillag varieties from the crop year 2011/2012. The experiment period was between July and August 2012 (24. 07. 2012., 31. 07. 2012., 21. 08. 2012.).We determined the rheological parameters (water absorption, dough stability time and valorigraph quality number) of Lupus and GK Csillag during short term storage. Our results showed that after-ripening increased the baking quality of Lupus and GK Csillag during storage.

Evaluating the effect of storage on the biological activity and chemical composition of three South African medicinal plants

Despite the popularity of traditional medicine in Africa, the continent remains behind in terms of the regulation, safety and quality control of its medicinal plant industry. There is heightened interest in essential aspects that affect the industry, with storage practices and consequent effects on plant efficacy being of high priority. Therefore, the effect of short-term storage on the efficacy and phytochemical content of three popular medicinal plants (Ocimum basilicum, Senna petersiana and Hypoxis hemerocallidea) was investigated. The antimicrobial and antioxidant activities of four solvent extracts of the selected plant (fresh and short-term stored) were compared. Phytochemical analysis of 50% methanol extracts of plant materials was determined using spectrophotometric methods. Fresh samples of the majority of the plant extracts indicated better antibacterial (Staphylococcus aureus and Escherichia coli) and antifungal (Candida albicans) activities in comparison to the stored samples. There was no specific pattern in antioxidant activity for both stored and fresh samples for the different plants. Phytochemical analysis pointed towards noticeable differences between fresh and stored samples of the various plant extracts with fresh samples showing higher chemical concentrations in many plant parts. In general, the current findings indicate that the degree of changes in the pharmacological and phytochemical activity due to storage was plant part- and species-specific.

Influence of short-term storage on electrophoretic profile of bovine serum proteins

Storage represents a basic step of the pre-analytical phase for the accuracy of screening tests both in human and veterinary diagnostic laboratories. This study aimed to evaluate the effect of short-term storage on electrophoretic profile of serum proteins in dairy cows. Blood was collected from 18 clinically healthy cows and serum was divided into four aliquots. The first aliquot was immediately analysed (time 0). The second and the third aliquots were stored at +4°C for 24 and 48 h, respectively. The fourth aliquot was frozen at −20° C for 48 hours. A statistical analysis was performed on the obtained data. Albumin and α-globulins relative concentration and A/G ratio showed significant changes after 48 h storage. The results obtained in the current study showed that short-term storage influenced electrophoretic pattern of bovine serum proteins and the A/G ratio. Therefore, the refrigeration of bovine sera for 24 h at the most should be suggested to practitioners in order to obtain reliable serum protein concentrations as useful screening test in veterinary diagnostic laboratory.

Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm

The sperm of Russian sturgeon (A. gueldenstaedtii) and Siberian sturgeon (A. baerii) was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), DNA integrity and oxidative stress indices. Spermatozoa showed >50% motility during 6 days of storage with an average velocity of 133.12±15.4 to 87.9±11.23μm s−1 in both species. No motile spermatozoa were recorded after nine days of storage. Analysis of Russian sturgeon sperm showed no significant differences in DNA damage expressed as percent tail DNA and Olive Tail Moment for first three days of storage. In Siberian sturgeon significant differences in DNA damage were detected after two days of storage. The level of oxidative stress indices (TBARS, CP) and antioxidant activity (SOD) increased significantly with storage time in both species. Results of this study can be utilized for successful reproduction management and cryopreservation protocols of these endangered species.

Effects of Heating Conditions on the Thermal Denaturation of White Mushroom Suitable for Dehydration

White mushrooms suitable for drying are thermolabile materials. At present, the determination of thermal denaturation of dehydrated white mushroom mainly depends on the drying technology. To choose the appropriate dehydration technology for thermal analysis, differential scanning calorimetry (DSC) was used for determining the denaturing temperature range. White mushrooms at five different moisture content levels were chosen for the DSC tests. Five heating rates were used to observe the change of the denaturing ranges. Finally, the effects of short-term storage and instantaneous increases in temperature on the change of denaturing areas are discussed.

306 EMBRYO ANALYSIS IN FIELD MOET: HIGH SUCCESS RATE AFTER OVERNIGHT CULTURE OF MICROBLADE-BIOPSIED CATTLE EMBRYOS

Embryo storage before embryo transfer is a necessity in field MOET to enable complex embryo analysis. Cryopreservation of biopsied embryos may reduce pregnancy rates to unacceptable levels, below 40% (Shea BF 1999 Theriogenology 51, 841–854). Our primary objective was to investigate the effect of short-term storage in overnight culture on the pregnancy rate of microblade-biopsied and sexed cattle embryos. A specific aim was to apply embryo storage in culture to marker-assisted selection of MOET embryos. Day 6.5 embryos were produced using standard superovulatory, AI, and embryo flushing procedures. Embryo donors were lactating cows of top genetic merit. Embryos were transported in straws in Holding medium (ICPbio, Auckland, New Zealand) to the laboratory and individually biopsied by a microblade. Biopsied embryos were cultured overnight in individual oil-overlaid 20-μL drops of Medium 199 with glutamax-1 (GIBCOTM, Baisley, UK) containing 0.25 mM sodium pyruvate, antibiotics, and 4 mg mL−1 fatty acid-free albumin. The biopsies were lysed in proteinase K and analyzed for sex with the BOV-Y/kappa-casein PCR method (Peura et al. 1991 Theriogenology 35, 547–555). Overnight-cultured grade I–II female embryos were transferred into the uteri of heat-synchronized recipients (Day 7.5). Pregnancy was confirmed by rectal palpation at 2–3 months after ET. Grade I-II male embryos as well as embryos of unknown sex were frozen in ethylene glycol, stored in liquid nitrogen, thawed, and cultured overnight for estimation of re-expansion rate and cell counts. In total, 74 embryos of eight donors were overnight-cultured and sexed. The success rate of the sexing method was 95%. Of the successfully analyzed embryos, 41% (29 of 70) were females and 59% (59 of 70) males. The survival rate of microblade-biopsied overnight-cultured embryos was 99% (73 of 74); 73% of the surviving embryos were of grade I, 23% of grade II, and 4% of grade III. Sixteen of the 27 (59%) grade I–II female embryos transferred resulted in pregnancy. Forty-two grade I–II embryos were frozen and 36 (86%) re-expanded after thawing and overnight culture. Twenty-four of the re-expanded embryos (67%) were of grade I. The re-expanded embryos had on average 73 cells (range 43–114). In conclusion, overnight culture of microblade-biopsied cattle embryos does not compromise embryo viability, resulting in high pregnancy rate and post-thaw re-expansion rate. The method can be utilized as a short-term embryo storage in the field MOET scheme and it will be applied in marker-assisted selection of MOET embryos for genotypes associated with milk production. The study was supported by the HAKA-Top breeding animals from North-Savo-project.

Effects of short-term storage on germinability of pistachio pollen

Effects of short-term storage on germinability of pistachio pollen