Effect Of Short-term Storage Research Articles

Effect of short-term storage of bovine oocytes in different media and temperatures on the subsequent in vitro embryo development

Effect of short-term storage of bovine oocytes in different media and temperatures on the subsequent in vitro embryo development

Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage

Spermatozoa and seminal plasma obtained from rainbow trout and whitefish were analyzed in respect to their aspartate aminotransferase (AspAT) and alkaline phosphatase activities. In particular, the experiments characterized AspAT optimum pH, optimization of assay conditions and action of coenzyme, pyridoxal 5-phosphate (vitamin B6). The effect of short-term semen storage at 0°C on biochemical indicators and fertilization rate was examined in both species. The concentrations of reduced and oxidized ascorbic acid in seminal plasma of both species were several folds higher than in spermatozoa and blood plasma of fish. Highly significant correlations were found for both species between AspAT activity (sperm or seminal plasma) and fertilization rate (% of eyed-stage or hatched embryos). For rainbow trout, highly significant correlations were found between sperm concentration, motility and fertilization rate. These results suggest that several biochemical indicators of seminal plasma can be used as measures of sperm quality of fish. Some common biochemical parameters for fish and mammal's semen provide evidence for using fish sperm as a model in biomedical research.

Effect of short term storage on plasma neuropeptide stability

Effect of short term storage on plasma neuropeptide stability

Effect of Short-Term Storage of Triadimefon-Treated Loblolly Pine Seed on Incidence of Fusiform Rust

Abstract Protection from fusiform rust, caused by Cronartium quercuum (Berk.) Miyabe ex Shirai f. sp. fusiforme Burdsall and Snow, on emerging seedlings of loblolly pine (Pinus taeda L.) was not diminished by storing the treated seeds up to 24 days before sowing. Young seedlings were inoculated with basidiospores of C quercuum f. sp. fusiforme 31 days after seeds were sown, and seedlings were examined for rust galls 7 months later. No galls were found on seedlings from seed dressed with triadimefon, regardless of length of storage. Percentages of seedlings with galls from seeds subjected to the triadimefon seed soak ranged from 12% (0 days storage) to 2% (24 days storage). Sixty-three percent of the seedlings from nontreated control seeds had galls. South. J. Appl. For. 12(1):18-20

The Effects of Short-Term Storage and Elevated Temperature on the Flocculation Activity of Aqueous Polymer Solution

The Effects of Short-Term Storage and Elevated Temperature on the Flocculation Activity of Aqueous Polymer Solution

The Effects of Short-Term Storage and Elevated Temperatures on the Flocculation Activity of Aqueous Polymer Solutions

Abstract Polyelectrolytes applied to water and waste treatment processes are generally assumed to be totally adsorbed to the sludge or solid phase. However it has previously been found that significant quantities of polyelectrolyte may appear in the effluent or liquid phase. This study developed a sensitive method, specifically a polystyrene latex test suspension, to detect trace concentrations of unmodified polyelectrolytes in distilled water as well as process effluents. A colloid titration method, as well as the test suspension were then used to assess the effects of storage at ambient and elevated temperatures. Both methods indicated that storage of polyelectrolyte at ambient and elevated temperatures could significantly reduce the effective charge (as measured by the colloid titration method) and the effective flocculating ability (as measured by the test suspension).

Neutrophil preservation: the effect of short-term storage on in vivo kinetics.

A rabbit model was developed to study the in vivo viability of neutrophils stored in vitro for up to 72 h. Acid-citrate-dextrose anticoagulated whole blood was obtained from rabbits previously injected with tritiated thymidine ([3H]thymidine), stored under varying conditions, and then injected into recipient rabbits. Neutrophil viability and function were assessed by measuring the ability of the tagged neutrophils to circulate and to migrate into subcutaneous polyvinyl sponges. Unstored neutrophils disappeared exponentially from the circulation with a t1/2 of 3.2 h and gave a zero time recovery of 30.5%. Storage of cells at either room temperature or 4 degrees C for 24 h or longer resulted in temporary sequestration of cells from active circulation. With cells stored for up to 72 h at 4 degrees C, recovery returned to normal values after 1-2 h. Room temperature stored cells, in contrast, showed evidence of irreversible damage at 48-h storage with low recovery for the entire time span studied. With unstored blood, 8.1+/-0.9% of the injected neutrophil label was present in the subcutaneous sponges. The accumulated label progressively decreased as cell storage time increased reaching at 72 h 5.1+/-0.6 and 2.6+/-0.3% for 4 degrees C and room temperature-stored cells, respectively. The results of this study indicate that 4 degrees C storage of rabbit neutrophils is superior to storage at room temperature. The data suggest that it may be feasible to store neutrophils at least a few days without loss of in vivo functions.

The effect of short term storage on the albumen quality of shell eggs

AbstractCertain physical changes occur naturally in the albumen of shell eggs when stored under cool conditions which cause the thick white to become thin. The whipping characteristics of the albumen are changed during this process but the functional performance of the albumen as measured by the angel food cake method is not affected to any marked degree.Three 10‐day storage trials were undertaken using day‐old shell eggs which demonstrated that a significant loss of albumen performance did not occur until the storage temperature reached 18 ºC. This conclusion clearly indicates the importance of maintaining shell eggs under cool conditions between collection at the farm and commercial distribution by the egg floors, particularly during the summer season.