In this study, the effect of ozonation process on the poly(ADP-ribose) polymerase 1 gene expression (PARP-1) and related the NADPH metabolism in strawberry fruit during storage was determined. Our results showed that ozonation with gas at both 10 and 100 ppm concentrations increased the expression of PARP-1 in the fruit during storage. Furthermore, the ozonation process initially increased the level of NAD+ and NADH in the fruit, which corresponds to a higher ATP level. The storage of the fruit in an ozone atmosphere also contributed to increased activity of the NAD+ kinase, leading to increased levels of NADP+ . In turn, the higher activity of glucose-6-phosphate dehydrogenase caused the ozonated fruit to show a higher level of NADPH. However, as the storage period extended and thus with increasing expression of PARP-1 in the ozonated fruit, the level of NAD+ decreased. In general, the ozonated fruit, which had a higher level of NADPH, showed a higher content of reduced glutathione, which in turn contributed to an increase in the antioxidant activity of the fruit and, ultimately, to a lower accumulation of reactive oxygen species.