Abstract
The major aim of this research was to investigate the effect of ozone treatment on the energy metabolism in raspberry fruit during storage at room temperature. Raspberries were ozonated with an ozone concentration of 8–10 mg L−1, for 30 min, every 12 h of storage at room temperature for 72 h. The results indicated that ozone treatment significantly enhanced the activities of mitochondrial respiratory enzymes, such as succinate dehydrogenase, cytochrome C oxidase, and H+-ATPase, which contributed to maintain the high level of ATP and energy charge in fruit during storage. Moreover, the energy metabolism in mitochondria was closely correlated with the antioxidant potential of raspberry fruit. This study has given an experimental evidence that ozonation procedure in proposed process conditions significantly affects the mitochondrial respiratory system leading to maintain the high quality of the fruit over a long period of storage at room temperature.
Highlights
Ozonation process is an alternative method for extending the postharvest shelf life of berry fruit
ATP level and energy charge of mitochondria are closely associated with the activity of enzymes participating in oxidative phosphorylation, including succinate dehydrogenase, cytochrome C oxidase, and H+-ATPase (Jin et al 2014; Jin et al 2013; Ge et al 2019; Li et al 2020; Zhou et al 2014)
Succinate dehydrogenase (SDH, energy charge (EC) 1.3.5.1) is the major enzyme of II complex in electron transfer chain (ETC) and catalyzes the dehydrogenation of succinate leading to fumarate generation, while cytochrome C oxidase (CCO, EC 1.9.3.1) catalyzes the reaction between ferricytochrome C and oxygen to yield ferricytochrome C and water (IV complex in ETC)
Summary
Ozonation process is an alternative method for extending the postharvest shelf life of berry fruit. Our previous study investigated the effectiveness of ozone treatment of raspberry fruit with 8–10 mg L−1 gas, for 30 min, every 12 h during storage at room temperature (72 h). Numerous scientific reports show that the resistance of the fruit to adverse changes in quality during storage is related to their energy metabolism (Zhou et al 2014). Scientific studies show that the use of various elicitors, e.g., chlorogenic acid, oxalic acid, 1-methylcyclopropene, or methyl jasmonate, increases the metabolic activity of mitochondria, which corresponds to better fruit quality during storage (Jin et al 2013, 2014; Li et al 2020; Shu et al 2020)
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