ABSTRACTEvaluation of any crop response to different nitrogen amounts is important for determining the amount that can be considered as optimum from economical and environmental point of view. This study was conducted to (1) evaluate the growth and yield of pumpkin (Cucurbita pepo L.) under different nitrogen rates and (2) determine the nitrogen use efficiency (NUE) of pumpkin in two growing seasons (2013 and 2014). In both growing seasons, nitrogen fertilizer (at three rates including 50, 150, and 250 kg ha−1) was band-dressed on the planted side of each furrow, coinciding with 4–6 leaves stage and flowering. Crop performance over 2 years was evaluated by measuring shoot dry matter, crop growth rate (CGR), leaf area index (LAI), leaf area duration (LAD), intercepted PAR (PARi), radiation use efficiency (RUE), shoot nitrogen uptake, water use efficiency (WUE), NUE, and fruit and seed yield. The results showed that in both growing seasons, the highest growth and yield of pumpkin were obtained by applying 250 kg N ha−1 (using urea fertilizer containing 46% nitrogen). Increased nitrogen rate from 50 to 250 kg ha−1 resulted in 87.3%, 27.0%, 62.1%, 87.5%, and 84.5% increase in shoot dry weight, RUE, WUE, fruit yield, and seed yield of pumpkin, respectively, across both growing seasons. However, higher application nitrogen rate decreased the NUE of pumpkin, i.e., the NUE decreased by 62.5% when the nitrogen rate increased from 50 to 250 kg ha−1. The effect of nitrogen applied in 2014 growing season on growth and yield of pumpkin was higher than that in 2013 growing season, which might be due to more suitable weather condition. In conclusion, the nitrogen rate of 250 kg ha−1 produced the highest amount of fruit and seed yield in pumpkin.