AbstractA pot experiment was conducted to determine the effects of N application on volatile compounds, taste compounds, and firmness of fresh tomato fruits. Each pot was filled with 8 kg of clean sand. The experiment consisted of six nitrogen (N)‐application rates with 0, 2.25, 4.50, 9.00, 18.00, and 36.00 mmol N L–1 in the nutrient solution. Volatile compounds, soluble sugars, soluble solids, titratable acidity, and firmness of fresh tomato fruits were measured. The results show that increasing N application increased the concentrations of 1‐penten‐3‐one, hexanal, cis‐3‐hexenal, 2‐methyl‐4‐pentenal, trans‐2‐hexenal, 6‐methyl‐5‐hepten‐2‐one, titratable acidity, soluble sugars, and soluble solids. By contrast, increasing N supply decreased the concentration of phenylacetaldehyde and first increased and then decreased the concentrations of 2E–4E‐hexadienal and the firmness of fresh tomato fruits. Close relationships between the concentration of various volatile compounds, titratable acidity, soluble sugars, and soluble solids were found. However, concentrations of these flavor compounds were very poorly correlated with fruit firmness. Based on contributions of these compounds to tomato flavor, we assume that moderate high N supply improves tomato flavor, whereas excessive N supply can deteriorate it.