Atom probe tomography (APT) has been used to study the effect of fast neutron irradiation on the local chemistry of Nb3Sn samples. Two RRP® wires doped with 2 at% Ti were analysed, one in the as-received condition and the other irradiated to a neutron fluence (E > 0.1 MeV) of 2.82 × 1022m−2 in the TRIGA-II reactor. The irradiated sample had a reduced T c, an increase in F p, a shift in the peak of the F p curve suggesting the introduction of secondary point pinning, and an increase in the estimated scaling field B*. APT analysis has shown that polycrystalline Nb3Sn has three distinct regions of composition, near stoichiometry Nb3Sn (low Nb), regions with a higher Nb content than expected in equilibrium Nb3Sn (high Nb) and grain boundaries. The summed composition of these three regions lies within the Nb3Sn phase for both the as-received and irradiated samples. The distinct regions of high Nb Nb3Sn demonstrate incomplete diffusion in the as-received sample, and the reduction in volume of these high Nb regions after irradiation implies significant radiation induced diffusion has occurred. The presence of other features in the atomic-scale chemistry, such as the extent of Cu segregation at grain boundaries, three types of dislocation array, and unreacted Nb nanoparticles, are compared between samples.