PurposeSustainable entrepreneurial ecosystem research is an emerging trend within the entrepreneurship domain. Drawing from resource dependency theory, this study examines the interdependent nature of sustainable entrepreneurial ecosystem factors and the mediating role of local culture as it relates to entrepreneurial action. The authors collected data from 12 entrepreneurial ecosystems in Australia and developed a model of the interdependencies of sustainable entrepreneurial ecosystem factors.Design/methodology/approachThe data were collected through an e-survey of Small and Medium Entreprise (SME) owners in New South Wales, Australia. The authors applied partial least squares structural equation methodology to assess the structural models, validate the outer models and examine the inner model.FindingsThe findings reinforce empirical support for sustainable entrepreneurial ecosystems. The environment where sustainable entrepreneurial ecosystems are evolved influences their functionality. Further, entrepreneurial culture mediates the relationship with other ecosystem factors. The theoretical and practical implications are discussed.Originality/valueThis study focuses on understanding the interdependent nature of sustainable ecosystem factors. The authors identified entrepreneurial culture as a mediator to business support services, educational institutional support and financial capital availability with business and social networks.