Size Effect Of Gold Nanoparticles Research Articles

Effect of Gold Nanoparticle Size on Regulated Catalytic Activity of Temperature-Responsive Polymer-Gold Nanoparticle Hybrid Microgels.

Gold nanoparticles (AuNPs) possess attractive electronic, optical, and catalytic properties, enabling many potential applications. Poly(N-isopropyl acrylamide) (PNIPAAm) is a temperature-responsive polymer that changes its hydrophilicity upon a slight temperature change, and combining PNIPAAm with AuNPs allows us to modulate the properties of AuNPs by temperature. In a previous study, we proposed a simpler method for designing PNIPAAm-AuNP hybrid microgels, which used an AuNP monomer with polymerizable groups. The size of AuNPs is the most important factor influencing their catalytic performance, and numerous studies have emphasized the importance of controlling the size of AuNPs by adjusting their stabilizer concentration. This paper focuses on the effect of AuNP size on the catalytic activity of PNIPAAm-AuNP hybrid microgels prepared via the copolymerization of N-isopropyl acrylamide and AuNP monomers with different AuNP sizes. To quantitatively evaluate the catalytic activity of the hybrid microgels, we monitored the reduction of 4-nitrophenol to 4-aminophenol using the hybrid microgels with various AuNP sizes. While the hybrid microgels with an AuNP size of 13.0 nm exhibited the highest reaction rate and the apparent reaction rate constant (kapp) of 24.2 × 10-3 s-1, those of 35.9 nm exhibited a small kapp of 1.3 × 10-3 s-1. Thus, the catalytic activity of the PNIPAAm-AuNP hybrid microgel was strongly influenced by the AuNP size. The hybrid microgels with various AuNP sizes enabled the reversibly temperature-responsive on-off regulation of the reduction reaction.

Gold nanoparticles with natural polymer synthesized by plasma–liquid interactions: Size-control, characterization and colorimetric detection of melamine based on the size effect of gold nanoparticles

This work reports the synthesis of size-controlled gold nanoparticles as AuNP colloids in aqueous solutions with capping natural polymer matrices – the sodium salt of carboxymethyl cellulose, using a liquid-phase plasma process. The effects of the solution plasma process discharge time, concentrations of gold(III) chloride trihydrate precursor (0.06–2.5 mmol/L) and correlations of the precursor/CMC ratios on the characteristics on the synthesized Au NPs, including size distribution and optical absorption properties, were investigated. The average size (20–90 nm) and form (spherical, hexagonal and triangular) of the AuNPs can be change depending on the initial concentration of Au3+ and the ratios of precursor/CMC on the process synthesis. Gold nanoparticles were used to study a straightforward colorimetric detection of melamine. Melamine caused the AuNPs to agglomerate in aqueous solution, changing their visible color from red to blue. The prepared AuNPs can be used for the detection of melamine with a low LOD and an extremely wide linear range (0.005–0.21 μM.).

Development of portable sensor for the detection of bacteria: effect of gold nanoparticle size, effective surface area, and interparticle spacing upon sensing interface

In this study, systematic development of a portable sensor for the rapid detection of Escherichia coli (E. coli) and Exiguobacterium aurantiacum (E. aurantiacum) was reported. A conductive glass was utilized as a substrate and developed the electrode patterns on it. Trisodium citrate (TSC) and chitosan-stabilized gold nanoparticles (AuNPs) (CHI-AuNP-TSC) and chitosan-stabilized AuNPs (CHI-AuNP) were synthesized and utilized as a sensing interface. The morphology, crystallinity, optical properties, chemical structures, and surface properties of immobilized AuNPs on the sensing electrodes were investigated. The sensing performance of the fabricated sensor was evaluated by using an electrochemical method to observe the current changes in cyclic voltammetric responses. The CHI-AuNP-TSC electrode has higher sensitivity toward E. coli than CHI-AuNP with a limit of detection (LOD) of 1.07 CFU/mL. TSC in the AuNPs synthesis process played a vital role in the particle size, the interparticle spacing, the sensor’s effective surface area, and the presence of CHI around AuNPs, thus enhancing the sensing performance. Moreover, post-analysis of the fabricated sensor surface exhibited the sensor stability and the interaction between bacteria and the sensor surface. The sensing results showed a promising potential for rapid detection using a portable sensor for various water and food-borne pathogenic diseases.

Effect of gold-nanoparticle size on microfiber saturable absorber for mode-locked erbium-doped fiber lasers

The optical performance of passively mode-locked erbium-doped fiber laser incorporating gold-nanoparticles (Au-NPs) of different sizes as saturable absorbers (SAs) was compared. A composite of Au-NPs with polydimethylsiloxane was spin coated on the surface of a microfiber waveguide. The fabricated Au-NP-based SA was deployed in a ring cavity erbium-doped fiber laser and its lasing performance was evaluated by comparing SA characteristics as well as pulse qualities among the different Au-NP sizes. The optimum pulse performance was realized when the SA was fabricated with 20 and 40 nm Au-NP size. For the former, time-bandwidth product of 0.34 was demonstrated which was the closest to its bandwidth-limited pulse. As for the latter, the fabricated SA exhibited 4.0% modulation depth and an average pulse duration of 886.7 fs. The research work has demonstrated the functionality of Au-NPs as a saturable absorption material for ultrashort pulses generation. In addition, the influence of nanomaterial size towards the characteristics of saturable absorbers, ergo the quality of laser pulse, was verified. This finding is also valuable in customizing ultrashort pulse output that can effectively suit system performance need.

Size effect of gold nanoparticles in bimetallic ZIF catalysts for enhanced photo-redox reactions

It is well known that gold nanoparticles have been widely used in various photo-redox reactions because of the unique surface plasmon resonance effect and the excellent internal electron transfer ability. However, the essential factors of how the metal nanoparticles enhancing the activity of photocatalysts have not been fully revealed. In this paper, gold colloidal particles of different sizes were modified on the surface of the dodecahedral skeleton of ZIF-Co/Zn. The photocatalytic activity of Au@ZIF-Co/Zn was investigated under simulated visible light for treating Rhodamine B/Cr(VI) mixture solutions (RhB/Cr(VI) 20 mg/L) and 4-nitrophenol solution (4-NP 20 mg/L). The results indicate that the size of gold nanoparticles could affect the surface plasmon resonance effect observably, internal electron transfer capacity and carrier density change. Photoelectric chemistry experiments and radical quenching experiments were performed to reveal the charge-transfer photocatalytic mechanism and size influence of gold nanoparticles in composite photocatalysts. This work may provide a novel strategy for the construction of metal–organic frameworks for the purification of wastewater mixed with heavy metal ions, organic pollutants and pharmaceutical intermediates.

Size effect of gold nanoparticles on optical and electrical properties of plasmonic silicon solar cell

One of important tasks of the day is increasing the efficiency and decreasing the cost of the silicon solar cells. There is method of introducing of metal nanoparticles into solar cells to improve its absorption and reduce transmission as well as reflection coefficients. When metal nanoparticles are introduced into silicon solar cell, nanoplasmonic effect will occur. Nanoplasmonic effect lead to modification of light spectrum and generation of extra hot electrons. Nano-plasmonic effect strongly depends on size of nanoparticles. Therefore, in this paper, effect of gold nanoparticles size on properties of silicon solar cell has been studied by using simulation. Gold nanoparticles with sizes of 4 nm, 6 nm, 9 nm, 11 nm and 21 nm have been input into emitter region of silicon solar cell in order to use both of nanoplasmonic-electric and nanoplasmonic-optic effects for enhancing efficiency of silicon solar cell. Open circuit voltage didn't change when size of nanoparticles has been changed from 4 nm to 11 nm. It dropped by 0.017 V when size of nanoparticles was 21 nm. Short circuit current has been maximum 6.7 mA/cm2 at nanoparticle size of 11 nm and minimum 3.1 mA/cm2 at nanoparticle size of 21 nm. It has been found from obtained results that gold nanoparticle with size of 11 nm affected significantly on properties of silicon solar cell. Besides, thickness of silicon solar cell can be decreased without dropping of efficiency by introducing gold nanoparticles. Because, main part of photons is absorbed near to metal nanoparticles inputted region.

Finite-difference time-domain to screen Au NPs as SERS active substrate for the sensitive determination of prohibited drugs in fish via solvent cleaning.

Surface enhanced Raman spectroscopy (SERS), as a molecule-specific method using plasmonic nanostructures to significantly enhance signal intensity, has been employed in various fields. In our study, we investigated the size effect of gold nanoparticles (Au NPs) on surface plasmon response by finite-difference time-domain (FDTD) simulation. In addition, SERS experiments, using the same concentrations of crystal violet (CV), were also carried out to confirm the simulation results. On this basis, the size of citrate-stabilized Au NPs (∼100 nm) was controlled by a seed-mediated growth, thus providing great electromagnetic field enhancement for SERS detection of CV in fish. Methanol solvent cleaning along with high speed centrifugal separation was developed, which could not only remove lipids in fish, but also produce hot spots via induced aggregation of Au NPs. The SERS detection limit of CV in fish could be less than 1 ppb. Such cost-effective and facile routes will be attractive for the trace detection of various analytes in complex matrices.

Effect of Gold Nanoparticles Size Capped with Surfactant on the Transformation of Plasmid into Escherichia coli Bacteria

Bacterial transformation has great importance in molecular biology, as it is used for introduction of foreign genes into bacterial cells either chemical or physical ways. Using calcium chloride to prepare competent cells and heat shock is the most widely used method for bacterial transformation. This method is an efficient and convenient technique but it has in some extent low transformation efficiency. Here we report the use of nanoparticles that significantly improve the transformation efficiency up to 10 times higher than the standard heat shock method by the assistance of (˜ 15, 25 nm) SDS capped gold nanoparticles in the transformation process that leads to the formation of temporary nano-channels across the bacterial cell wall to deliver plasmids into cells. Transformation of bacteria with plasmid was examined using Β-galactosidase assay.

Effect of Gold Nanoparticles Size on Detection of Methylphosphonic Acid Hydrolysis Product of Nerve Agent

Citrate reduction of gold (III) chloride trihydrate (HAuCl4) is commonly used method to synthesise citrate-capped gold nanoparticles (cit-AuNPs). In this study, the sequence of reagents addition was modified (“inverse” method) to synthesise smaller size of cit-AuNPs than the standard Turkevich method (“direct” method). Ultraviolet-visible spectroscopy (UV-vis) and field emission transmission electron microscopy (FETEM) confirmed the formation of cit-AuNPs. The cit-AuNPs synthesized using “inverse” method are smaller in size (14.0 ± 3.03 nm) with uniform spherical shape compared to “direct” method (23.5 ± 7.52 nm). Smaller particles size of cit-AuNPs provide higher efficiency and sensitivity for detection of methylphosphonic acid (MPA) via colorimetric incorporated with image processing with a linear range from 2.5 to 12.5 mM and a low detection limit of 6.28 mM at shorter detection period (24 to 30 s).

Experimental studies on evaporation kinetics of gold nanofluid droplets: Influence of nanoparticle sizes and coating on thermal performance

In this work, a series of experiments investigate the evaporation of gold nanofluid sessile droplets on a perfluorodecyltrichlorosilane (PFTS) silicon substrate heated to 77 °C. The evaporation processes of different nanofluid droplets of the same initial volume, all for a 1% Cv volume concentration prepared from the 0.1 mM original suspension, are visualized to examine the size effect of gold nanoparticles (2.2, 5 and 10 nm) and the impact of surface coating (with and without Citrate capping in phosphate-buffered saline solvent) on enhancing heat transfer. This study open access to understand the size effect of gold nanoparticle in very small scale and the different types of surfactant on heat and mass transfer during the evaporation of droplet. Two methods are used to analyze the evaporation process, an optical one coupled to an infrared thermography method and an acoustic method. These complementary methods have the ability to investigate clearly the solid/liquid and liquid/vapor interfaces at the same time. From the optical observation, using a Drop Shape Analyzer (Kruss system), the evolution in time of the shape of the droplets (contact angle, base diameter and volume) are measured under controlled conditions (Humidity = 50%, Tatm = 23 °C). Then, the evaporation rate is deduced from the measurements of the evolution of volume in time. At the same time, an infrared camera is used to observe the droplet surface gradient temperature, air/liquid interface, due to thermal Marangoni flow. The acoustic method, based on a high- frequency echography principle, allowed to monitor the stability of nanoparticles inside the droplets (Au-water mixture) during the process of evaporation. From the kinetics of the reflection coefficient at the interface of the silicon substrate over which the nanofluids are deposited, the results show that the evaporation rate of 5 nm Au-water mixture (phosphate buffered saline solvent only) was faster than that of 10 nm Au-water mixture. While with citrate capping-PBS, the evaporation rate of the largest particle sizes (10 nm Au-water mixture) was the fastest than that of the smaller sizes (2.2 and 5 nm). Also, the 5 nm gold nanofluids with PBS showed the highest evaporation rate (+35%) than that of citrate capping gold nanofluids (+15%). As results, the addition of a surfactant (citrate capping) decreases the effectiveness of the heat transfer of gold nanofluids. This seems to be due to the absence of Marangoni cells in droplets made of Au-water mixtures (Citrate capping-PBS). Moreover, reducing in the thermal conductivity of gold nanofluid due to the surfactant coating by covering the gold nanoparticles surface and this eventually affected the nanofluid thermal properties. This is further supported by the acoustic method, which clearly shows worse stability of the nanoparticles in the latter case.

Effect of Gold Nanoparticle Size on Their Properties as Contrast Agents for Computed Tomography

Computed tomography (CT) is one of the most commonly used clinical imaging modalities. There have recently been many reports of novel contrast agents for CT imaging. In particular, the development of gold nanoparticles (AuNP) as CT contrast agents is a topic of intense interest. AuNP have favorable characteristics for this application such as high payloads of contrast generating material, strong X-ray attenuation, excellent biocompatibility, tailorable surface chemistry, and tunable sizes and shapes. However, there have been conflicting reports on the role of AuNP size on their contrast generation for CT. We therefore sought to extensively investigate the AuNP size-CT contrast relationship. In order to do this, we synthesized AuNP with sizes ranging from 4 to 152 nm and capped them with 5 kDa m-PEG. The contrast generation of AuNP of different sizes was investigated with three clinical CT, a spectral photon counting CT (SPCCT) and two micro CT systems. X-ray attenuation was quantified as attenuation rate in Hounsfield units per unit concentration (HU/mM). No statistically significant difference in CT contrast generation was found among different AuNP sizes via phantom imaging with any of the systems tested. Furthermore, in vivo imaging was performed in mice to provide insight into the effect of AuNP size on animal biodistribution at CT dose levels, which has not previously been explored. Both in vivo imaging and ex vivo analysis with inductively coupled plasma optical emission spectroscopy (ICP-OES) indicated that AuNP that are 15 nm or smaller have long blood circulation times, while larger AuNP accumulated in the liver and spleen more rapidly. Therefore, while we observed no AuNP size effect on CT contrast generation, there is a significant effect of size on AuNP diagnostic utility.

Effect of Gold Nanoparticle Size on Bacterial Cell Attachment and Initial Biofilm Formation

Effect of Gold Nanoparticle Size on Bacterial Cell Attachment and Initial Biofilm Formation

The Effect of Gold Nanoparticle Size in the Cellular Uptake

Nowadays, gold nanoparticles (AuNPs) received a great deal of attention for biomedical applications especially in diagnostic imaging and therapeutics. In this study, we elucidate on how size of gold nanoparticles (AuNPs) affect cellular uptake. The size dependence of AuNPs cytotoxicity in MCF-7 cells was tested by using the WST-1 assay. The sizes of AuNPs tested were 13 nm, 50 nm, and 70 nm. The toxicity of AuNPs in MCF-7 cell lines was quantified by determining the IC50 values in WST-1 assays. The IC50 values (inhibitory concentrations that affected 50% growth inhibition) of 13 nm AuNPs is higher than 50 nm and 70 nm AuNPs. Mean that, 13 nm AuNPs is less toxic to MCF-7 cells. This cytotoxicity results generally agree with those obtained in the study of cellular uptake by Inverted Microscope and EFTEM. Morphological observation of MCF-7 after being treated with 13 nm, 50 nm, and 70 nm AuNPs, were looking unhealthy and dying out of the populace, the observed cells were more reduced and dying as treatment with 50 nm and 70 nm AuNPs. Cells detachment, clumping, shrunken, and dispersed cells in the culture medium and floating cells were also observed. The observed morphological changes increase in 50 nm and 70 nm AuNPs than in 13 nm AuNPs, which is less toxic to MCF-7 cells. The presented morphological analysis has also established that 13 nm AuNPs showed less toxic to MCF-7 cells. The presented results clearly indicate that the cytotoxicity and cellular uptake of AuNPs depend on the size of the nanoparticles.

Effect of Gold Nanoparticle Size and Coating on Labeling Monocytes for CT Tracking.

With advances in cell therapies, interest in cell tracking techniques to monitor the migration, localization, and viability of these cells continues to grow. X-ray computed tomography (CT) is a cornerstone of medical imaging but has been limited in cell tracking applications due to its low sensitivity toward contrast media. In this study, we investigate the role of size and surface functionality of gold nanoparticles for monocyte uptake to optimize the labeling of these cells for tracking in CT. We synthesized gold nanoparticles (AuNP) that range from 15 to 150 nm in diameter and examined several capping ligands, generating 44 distinct AuNP formulations. In vitro cytotoxicity and uptake experiments were performed with the RAW 264.7 monocyte cell line. The majority of formulations at each size were found to be biocompatible, with only certain 150 nm PEG functionalized particles reducing viability at high concentrations. High uptake of AuNP was found using small capping ligands with distal carboxylic acids (11-MUA and 16-MHA). Similar uptake values were found with intermediate sizes (50 and 75 nm) of AuNP when coated with 2000 MW poly(ethylene-glycol) carboxylic acid ligands (PCOOH). Low uptake values were observed with 15, 25, 100, and 150 nm PCOOH AuNP, revealing interplay between size and surface functionality. Transmission electron microscopy (TEM) and CT performed on cells revealed similar patterns of high gold uptake for 50 nm PCOOH and 75 nm PCOOH AuNP. These results demonstrate that highly negatively charged carboxylic acid coatings for AuNP provide the greatest internalization of AuNP in monocytes, with a complex dependency on size.

Effect of gold nanoparticle size and surface coating on human red blood cells

This paper reports the impact of bovine serum albumin (BSA) coating on gold (Au) nanoparticles (NPs) with sizes of 15, 30, 50 and 70 nm on cellular uptake and haemolysis of human red blood cells (RBCs). BSA coating on gold NPs imparts extra stability in high-glutathione-containing medium, which is a major prerequisite for NPs being developed for delivery applications. BSA coating on gold NPs was characterised by Fourier transform infrared spectroscopy, whereas cellular uptake was estimated by ultraviolet–visible spectrophotometry and flow cytometry. The cellular uptake results show that the internalisation of bare gold NPs is size dependent; however, upon BSA conjugation, uptake becomes independent of particle size. Cytocompatibility of bare and BSA-coated gold NPs was assessed by MTT assay, a common method to evaluate the biocompatibility of nanomaterials, and found non-toxic. However, when bare gold NPs were exposed to human RBCs, the NPs exerted significant haemolysis, which suggests that bare gold NPs which are considered as non-toxic to mammalian cells, can be harmful to RBCs. Interestingly, BSA-coated gold NPs showed significantly lower haemolysis at similar concentrations, suggesting that BSA-coated gold NPs could be of great importance in biomedical applications.

Probing the Localized Surface Plasmon Field of a Gold Nanoparticle-Based Fibre Optic Biosensor

Gold nanoparticles (GNP) have been used in a variety of localized surface plasmon resonance (LSPR)-based optical sensor systems and in a variety of forms, such as colloidal suspensions, immobilized GNP on flat surfaces or optical fibres. A key parameter affecting the sensitivity of these systems is the effective depth of penetration of the surface plasmons. This study aims to determine the plasmon penetration depth in the case of an immobilized GNP-based LSPR optical biosensor. The optical biosensor used for experimentation is a U-bend fibre optic probe of 200-μm core diameter and 1.5-mm bend diameter on which GNP is immobilized. Formation of multilayered nanostructures on the immobilized GNP was used to investigate the field of the localized surface plasmons. Two multilayered nanostructures were explored in this study, viz. a polyelectrolyte multilayer formed by layer-by-layer (LBL) deposition of oppositely charged polyelectrolytes and an immunoglobulin G (IgG) multilayer formed through sequential immobilization of two mutually specific antibodies. Measurement of LSPR absorbance change with deposition of each analyte layer was used to determine the plasmon penetration depth (d P) of the LSPR biosensor. Probing the plasmon field with an IgG multilayer gave rise to at least twofold higher d P compared to d P obtained from the polyelectrolyte multilayer. The effect of GNP size was also studied, and GNP of three diameters, viz. 18, 36 and 45 nm, were used. The 36-nm-diameter GNP exhibited the highest d P. The outcomes of this study may provide leads for optimization of LSPR-based sensors for various biosensing applications.

Coassembly of gold nanoparticles and cellulose nanocrystals in composite films.

Coassembly of nanoparticles with different size-, shape-, and composition-dependent properties is a promising approach to the design and fabrication of functional materials and devices. This paper reports the results of a detailed investigation of the formation and properties of free-stranding composite films formed by the coassembly of cellulose nanocrystals and shape-isotropic plasmonic gold nanoparticles. The effect of gold nanoparticle size, surface charge, and concentration on the structural and optical properties of the composite films has been studied. The composite films retained photonic crystal and chiroptical activity properties. The size and surface charge of gold nanoparticles had a minor effect on the structure and properties of the composite films, while the concentration of gold nanoparticles in the composite material played a more significant role and can be used to fine-tune the optical properties of materials derived from cellulose nanocrystals. These findings significantly broaden the range of nanoparticles that can be used for producing nanocomposite materials based on cellulose nanocrystals. The simplicity of film preparation, the abundance of cellulose nanocrystals, and the robust, free-standing nature of the composite films offer highly advantageous features and pave the way for the generation of functional materials with coupled optical properties.

The effect of gold nanoparticle size on osteogenic differentiation of adipose-derived stem cells

There have been many medical applications based on gold nanoparticles (GNPs) over the past several centuries. Recently, researchers have focused on bone tissue engineering applications utilizing GNPs. The effect of various sizes of gold nanoparticles on the differentiation of human adipose-derived stem cells (ADSCs) into osteoblasts was investigated. The concentration of gold nanoparticles was fixed at 1μM and varying sizes of 15, 30, 50, 75 and 100nm (spherical GNPs) were used. The lack of cytotoxicity was confirmed by establishing viability of ADSCs using cell counting kit-8 (CCK-8) and live/dead assays. The results showed that each size of GNPs had no significant toxicity on ADSCs during 1week of incubation. Osteogenic differentiation of ADSCs was confirmed by alkaline phosphatase (ALP) staining, ALP activity, calcium deposition, and real time PCR experiments. It was found, through dark field assays and microscope cell images, that 30nm and 50nm GNPs were preferentially up taken into the ADSCs. As expected, all sizes of gold nanoparticles promoted the differentiation of ADSCs toward osteoblasts more than control. Among all sizes, 30 and 50nm GNPs appeared to have the highest differentiation rates. The data consistently demonstrated that 30 and 50nm GNPs are the most effective in promoting osteogenic differentiation of ADSCs.

Size effect of gold nanoparticles supported on carbon nanotube as catalysts in selected organic reactions

Carbon nanotube-supported gold nanoparticles of different sizes (diameter of 3 or 20 nm) were evaluated as catalysts in four selected organic transformations. The nanohybrids were shown to efficiently catalyze the investigated reactions, regardless of the size of the supported gold nanoparticles. However, some differences were observed as regards turnover frequency values although size effect turned out to be less significant when only gold surface atoms were considered.

Size Effect of Gold Nanoparticles in Catalytic Reduction of p-Nitrophenol with NaBH4

Gold nanoparticles (Au NPs) were prepared by reducing HAuCl4 with NaBH4. Their average particle sizes could be tuned in the range of 1.7 and 8.2 nm, by adjusting the amount of NaBH4 used during synthesis. The obtained Au NPs (colloids) were then loaded onto a commercial Al2O3 support to prepare Au/Al2O3 catalysts with tunable Au particle sizes. An optimal pH value (5.9) of the Au colloid solution was found to be essential for loading Au NPs onto Al2O3 while avoiding the growth of Au NPs. Au NPs and Au/Al2O3 catalysts were tested in the reduction of p-nitrophenol with NaBH4. Interestingly, the catalytic activity depended on the size of Au NPs, being the highest when the average size was 3.4 nm. Relevant characterization by UV-Vis, TEM, and XRD was conducted.