Objective: To assess the physiological effects of examination stress on red blood cells in female medical students at Peoples university of medical and health sciences for women, Nawabshah. Study Design: Comparative Cross Sectional study. Setting: Department of Physiology, Peoples University of Medical & Health Sciences for Women, Shaheed Benazir Abad. Period: 7th April 2019 to 6th October 2019. Methods: A total 110 healthy students of first & Second year MBBS were included for this, by the non-probability randomized sampling technique, divided into two equal (55-students in each) groups A and B first & second year MBBS. Blood samples were collected prior to and during the exam, and the findings were evaluated using statistical software. Results: The mean RBC count ± SD thru the examination of students including the first year was 4.98±0.26 c/cm3, which was considerably higher than the pre-exam mean RBC count ±SD of 4.65±0.27 c/cm3. There was a small rise in RBC count throughout the examination compared to before (n = 55, p-value = 0.031) among the first-year students. During the second year examination, students had a mean RBC count ± SD of 4.67±0.28 c/cm3, which was not substantially higher than the pre-exam mean of 4.66±0.33 c/cm3. There was no significant increase in RBC count throughout the examination compared to before it among 2nd year students (n = 55, p-value = 0.932). Conclusion: Findings of study concluded that examination stress significantly affects first-year medical students' red blood cell (RBC) count, increasing it during exams, but not second-year students. This suggests varying physiological responses to stress, possibly due to academic adaptation or stress levels.
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