Effects Of Estradiol Benzoate Research Articles

SOME ASPECTS OF THE METABOLISM OF SULFATE-S35 AND CALCIUM-45 IN THE METAPHYSES OF IMMATURE RATS

Weanling rats were given 2 mg. of 17-beta-estradiol benzoate at weekly intervals for 4 weeks. Twenty-four hours after each intraperitoneal injection of the estrogen 100 microc. of S(35)-sulfate or 11 microc. of Ca(45) was similarly injected. The animals were sacrificed 24 hours after the last dose of isotopes. An effect of estradiol benzoate on calcium metabolism was deduced from the observation that the concentration of calcium in some tissues of the treated rats was higher than the concentration in the tissues of untreated rats. Alkaline extracts of the distal metaphyses of femurs from the estradiol-treated and from control rats, given S(35)-sulfate, were shown by chromatography on an anion exchange resin to contain from 9 to 22 per cent of the S(35) as inorganic sulfate. From similar bone samples, 6 to 21 per cent of the S(35) was removed by decalcification with sodium versenate. Most of the remaining S(35) was associated with uronic acid and hexosamine; on paper chromatograms and paper electrophoretograms S(35) was shown to be part of material which migrated and was metachromatic in the same way as purified chondroitin sulfate. Autoradiograms of the proximal ends of tibiae from the animals given estradiol benzoate showed that both the S(35) and Ca(45) were deposited in the metaphyses in strata. The arrangement of the strata of S(35), however, was different from the arrangement of the strata of Ca(45). This difference in arrangement is interpreted as indicating that most of the S(35) in the metaphysis was derived from the chondroitin sulfate of the cartilage plate which the metaphysis had replaced.

Effect of oestradiol benzoate and orchidectomy on the toxicity of trypan blue.

PRISELKOV1 found that the toxic effect of trypan blue was manifested in guinea pigs by loss of weight and hair, apathetic appearance, fall in haemoglobin content, accumulation in the blood of large numbers of polychromatophiles and normoblasts and the appearance of erythrocyte decomposition products in many organs. Nicol2 pointed out that some animals appeared to be more susceptible to the toxicity of the dye during œstrus. The toxic effect of injections of trypan blue on the tissues has been studied histologically by Downey3, who observed that the cells of connective tissue first showed diffuse staining of the cytoplasm and the nucleus which he considered to be the appearance of dying cells. Many cells died, but some were able to eliminate the dye and recover, except the eosinophiles. The reticulo-endothelial cells, which specifically store the vital dye, are affected in the same manner, and it is probable that the resulting inhibition of the functional capacity of the reticulo-endothelial system is partly responsible for the toxic manifestations of trypan blue.

Observations on the effect of estradiol benzoate on the incorporation of radioactive phosphorus in the vagina of the rat

Observations on the effect of estradiol benzoate on the incorporation of radioactive phosphorus in the vagina of the rat

THE EFFECT OF ESTROGEN ON THE PERMEABILITY OF THE UTERINE CAPILLARIES1

FOR SOME TIME it has been known that estrogens produce fluid accumulation in the uterus of experimental animals. Although the length of the latent period following estrogen administration (1) and the electrolytic shifts involved (2) have been evaluated, the mechanism by which the edema reaction proceeds is obscure. In general, the principal factors which favor enhanced fluid accumulation have been listed as follows (3): (a) increases in the capillary pressure, (b) increases in the permeability of the capillary membranes, (c) reduction in the osmotic pressure of plasma as compared to tissue fluid, (d) obstruction of the lymph channels. To produce uterine edema, estrogen must set into motion one or more of the above factors or some new mechanism hitherto not described. In an attempt to elucidate the mechanism of the uterine fluid accumulation induced by estrogen, we have tried to evaluate, in part, the effect of estradiol benzoate on the factors producing edema.

Effect of Oestradiol Benzoate on the Amount of Gonadotrophin in Pituitary Gland and Urine of Post-menopausal Women

Effect of Oestradiol Benzoate on the Amount of Gonadotrophin in Pituitary Gland and Urine of Post-menopausal Women

Hypophyseal tumors induced by estrogenic hormone.

The effects of estradiol benzoate (EB) injection on sexual development and the pituitary gland were studied in 240 immature rats after 16 weeks of treatment. Treatment resulted in eunuchoid dwarfing. Males showed enlargement of the pituitary after 4 months, while in females it remained normal. After continued treatment for 8 months, enormous hypophyseal tumors were observed in 29 of 35 rats of both sexes; 1 animal died as a result of these tumors. No tumor developed in less than 7 months, which suggests that the duration of treatment, rather than the dose, is of primary importance in tumorigenesis. The amount of gonadotropic hormones in the enlarged pituitaries and tumors was the same as in normal pituitaries of control animals, which indicates that it is the utilization, rather than the production, of gonadotropic hormones that is inhibited by EB-induced eunuchoid dwarfs.