Effect Of Different Media Research Articles

Effect of CTAB and NaBH4 Solutions on Ti and Cu Nanoparticles Prepared by Pulsed Laser Ablation

The effect of different media, including cetyltrimethylammonium bromide (CTAB) and sodium borohydride (NaBH4), on the size, shape, optical absorption, and stability of titanium (Ti) and copper (Cu) nanoparticles (NPs) synthesized by laser ablation in liquid, was investigated. Ti and Cu NPs were fabricated by irradiating Ti and Cu targets using a Nd:YAG pulsed laser with a wavelength of 1064 nm, an energy of 500 mJ, repetition rate of 1 Hz, and a number of pulses of 1000. Results showed the direct effect of the type of surfactant on the size and size distribution of Ti and Cu NPs under the same laser ablation parameters. Morphological properties were described by field emission scanning electron microscope (FESEM) images, which revealed the formation of semispherical particles. The EDS results confirmed the spike-induced synthesis of Ti and Cu in CTAB and NaBH4 solutions. The transmission electron microscopy (TEM) images showed the nanostructures of Ti and Cu with spherical particles. The UV-vis absorption spectra showed that the absorption peak of Ti was almost constant at 289 nm, while the peak of Cu disappeared for both solutions. Zeta potential analysis showed that NPs prepared in CTAB solution were more stable than those in NaBH4 solution.

Effects of different culture media on growth, composition, quality and palatability of the green algae Ulva sp. cultivated in cylindrical photobioreactors

Ulva spp. are valuable seaweeds with recognized commercial applications, including food, feed, and ecosystem services. Ensuring a sustainable and consistent supply of biomass with desirable profiles aligned with intended uses is fundamental for the successful applications of this seaweed. In this study, the growth rate, morphology, physiology, and composition of Ulva sp. produced by propagation in indoor cylindrical photobioreactors using four different culture media (lagoon water - LW, lagoon water enriched with Guillard medium (LF), with sea urchin wastewater - LU, and cow digestate - LD) was assessed; moreover, the nutrient uptake potential of the species was evaluated. The palatability and attractivity of the produced biomass towards the sea urchin Paracentrotus lividus were investigated. It was found that the media influenced all the parameters examined, the LF biomass weight was double compared to the other treatments and showed a slightly higher absorbance. Colorimetric analyses reported a significant darker color in Ulva sp. grown under enriched media. Ulva sp. showed higher nutrient removal potential in LF. The lipid content did not vary (2–3 % dry weight, DW), while the protein content ranged from 21 % in LF to 6–9 % in the other treatments. Carbohydrates and fiber content were significantly lower in LF (16 % and 30 %) compared to the other treatments, 27–34 %, and 41–48 %, respectively. Pigment content significantly varied, being higher in biomass grown in LF and LU. Sea urchins showed preferences for biomass grown under LU, followed by LD. This study shows how different nutrient sources affect the biochemical composition, growth, quality, and palatability of Ulva sp.. When cultivated under the synthetic enriched media (LF) the species exhibits characteristics better suitable for human consumption, although requiring a higher economic investment for production, while biomass derived from wastewater nutrients (LD, LU) confirms potential applications of the seaweed as valuable feed and for bioremediation services.

Assessment of Chondrogenic Potential of Human Dermal Fibroblasts with Various Protein Modifications

BACKGROUND: Hyaline cartilage is an avascular tissue that covers surface of large joints. This tissue, with a small number of cells and a high content of extracellular matrix proteins, has limited regenerative capacity. Recovery the articular cartilage remains a relevant and not yet fully resolved issue today. A promising approach is the use of biomedical products containing modified cells. AIM: To investigate the effects of different culture media on the chondrogenic modification of cells and to compare the results. METHODS: Human dermal fibroblasts and rabbit mesenchymal stem cells were modified using chondrogenic differentiation medium StemPro or recombinant TGF-β3 protein. Changes in the expression of genes responsible for chondrogenesis (Acan, Tgfβ3, Col2α1, Comp) were measured using the Real-Time PCR method -2ΔΔCt. RESULTS: It was shown that human dermal fibroblasts are capable of chondrogenic modification using protein media. This cell type is easily accessible for harvesting, does not require special conditions for cultivation, is easily scalable, and can be used for allogeneic transplantation. CONCLUSION: The obtained data can be used in the design of tissue engineering products for the regeneration of hyaline cartilage based on allogeneic dermal fibroblasts.

Production of Citric Acid from Different Aspergillus species Obtained from Soil

The genus Aspergillus is considered highly important in the production of various types of enzymes and organic acids. Aspergillus species produce organic acids such as citric acid, itaconic acid, and malic acid, which are one of the most important alternate techniques for chemical processes. Citric acid is an essential primary agricultural product that is extensively used in the world. It acts as an intermediate in the tricarboxylic acid cycle (citric acid cycle) during the oxidation of carbohydrates to carbon dioxide. Citric acid has an important role in industries involving the production of food products, beverages, pharmaceutical and agricultural products, detergents, and cosmetics. The production of citric acid, a crucial agricultural commodity with widespread applications in various industries including food, beverages, pharmaceuticals, agriculture, detergents, and cosmetics, heavily relies on microbial fermentation. The great demand for citric acid due to its wide industrial applications and less toxicity. Citric acid can be produced using less expensive substrates that are renewable too. In this work, Aspergillus species was isolated from the soil source, collected in different localities of Chhatrapati Sambhajinagar. The total 90 different isolates are isolated among these the 20 different Aspergillus species are isolated and pure culture by using potato dextrose agar (PDA) Czapek dox agar medium and study the effect of different media on growth and sporulation of different Aspergillus species and the quantitative analysis of citric acid production was done by titration method with help of NaOH and phenolphthalein indicator. The main reason for the study increases is the large number of applications that can be found for citric acid, primarily in the food and pharmaceutical industries

Effects of different adding methods of fermentation medium on the quality of cigar.

Background: To investigate the effects of different media and media addition methods on the fermentation of tobacco. Methods: A plant extract and a bacterial agent are used for fermenting cigar tobacco leaves in a different order of addition. The chemical composition, cellulose, and pectin content, as well as changes in the microbial community, were measured. Results: The addition method of the fermentation medium affects the quality of fermented tobacco. The optimal medium formula and addition method involve first adding plant extracts and then microbial agents. The medium formula and addition method can significantly reduce cellulose in tobacco, with a reduction rate of 46%, and significantly increase the content of β-carotene, thereby enhancing the aroma of cigarettes. There is an increase in aroma components, such as alcohols, alkanes, and olefins, in tobacco. By reducing the proportion of Aspergillus, it can alter the microbial community structure of tobacco. Conclusion: Adding plant extracts before introducing microbial agents can significantly improve the quality and alter the microbial community structure of Dexue No.1 tobacco.

Effect of Different Media, Temperature, pH and Nitrogen Sources on Growth and Development of Helminthosporium oryzae Causing Brown Leaf Spot of Rice

Effect of Different Media, Temperature, pH and Nitrogen Sources on Growth and Development of Helminthosporium oryzae Causing Brown Leaf Spot of Rice

Optimization of the replication of hepatitis E virus genotype 3 in vitro.

Hepatitis E virus (HEV) is responsible for ∼20 million human infections worldwide every year. The genotypes HEV-3 and HEV-4 are zoonotic and are responsible for most of the autochthonous HEV cases in high-income countries. There are several cell culture systems that allow for propagation of different HEV genotypes in vitro. One of these systems uses human lung carcinoma cells (A549), and was further optimized for propagation of HEV-3 47832c strain. In this study, we investigated the effect of different media supplements as well as microRNA-122 (miR-122) on improving the replication of HEV-3 47832c in A549 cells. We observed that supplementation of maintenance media with 5% fetal bovine serum was sufficient for efficient replication of HEV-3, and verified the positive effect of media supplementation with Amphotericin B, MgCl2, and dimethyl sulfoxide on replication of HEV-3. We have also demonstrated that adding miR-122 mimics to the culture media does not have any significant effect on the replication of HEV-3 47832c. Herein, we detected over a 6-fold increase in HEV-3 replication in A549/D3 cells by adding all three supplements: Amphotericin B, MgCl2, and dimethyl sulfoxide to the culture media, while demonstrating that miR-122 might not play a key role in replication of HEV-3 47832c.

Influence of different culture media on the antimicrobial activity of Pediococcus pentosaceus ST65ACC against Listeria monocytogenes.

Pediococcus pentosaceus ST65ACC is a bacteriocinogenic lactic acid bacteria (LAB) isolated from Brazilian artisanal cheese that is capable of inhibiting different food pathogens, mainly Listeria monocytogenes. The production of bacteriocins can be influenced by several growth conditions, such as temperature, pH, and medium composition. This study aimed to evaluate the effect of different culture media on the production of bacteriocins and antimicrobial activity of P. pentosaceus ST65ACC on L. monocytogenes Scott A. The strains were inoculated alone and in coculture in four different media: BHI broth, MRS broth, meat broth, and reconstituted skim milk (RSM) 10% (w/v). The culture media were then incubated at 37°C for 96h, and count analysis, pH measurement, and bacteriocin production were performed at 0, 24, 48, 72 and 96h. L. monocytogenes was inhibited to nondetectable levels in coculture with P. pentosaceus ST65ACC in MRS broth within 96h, consistent with the high production of bacteriocin throughout the analysis period (3,200-12,800 AU/mL). However, lower inhibitory activities of P. pentosaceus ST65ACC on L. monocytogenes Scott A were recorded in BHI, RSM, and meat broth, with low or no production of bacteriocins at the analyzed times. The composition of these culture media may have repressed the production and activity of bacteriocins and, consequently, the antagonist activity of P. pentosaceus ST65ACC on L. monocytogenes Scott A. The results showed that the antimicrobial activity was more effective in MRS broth, presenting greater production of bacteriocins and less variability when compared to the other media analyzed.

Optimizing Cucumis sativus seedling vigor: the role of pistachio wood vinegar and date palm compost in nutrient mobilization.

The goal of this research is to enhance the quality of cucumber seedlings grown in greenhouses by experimenting with various soilless culture mediums (CMs) and the application of pistachio wood vinegar (WV). The experimental setup was designed as a factorial experiment within a randomized complete block design (RCBD), in greenhouse conditions featuring three replications to assess the effects of different culture media (CMs) and concentrations of pistachio wood vinegar (WV) on cucumber seedling growth. Cucumber seeds were planted in three CMs: coco peat-peat moss, coco peat-vermicompost, and date palm compost-vermicompost mixed in a 75:25 volume-to-volume ratio. These were then treated with pistachio WV at concentrations of 0, 0.5, and 1%, applied four times during irrigation following the emergence of the third leaf. The study revealed that treating seedlings with 0.5% WV in the date palm compost-vermicompost CM significantly enhanced various growth parameters. Specifically, it resulted in a 90% increase in shoot fresh mass, a 59% increase in shoot dry mass, an 11% increase in root fresh mass, a 36% increase in root dry mass, a 65% increase in shoot length, a 62% increase in leaf area, a 25% increase in stem diameter, a 41% increase in relative water content (RWC), and a 6% improvement in membrane stability index (MSI), all in comparison to untreated seedlings grown in coco peat-peat moss CM. Furthermore, chlorophyll a, b, total chlorophyll, and carotenoid levels were 2.3, 2.7, 2.6, and 2.7 times higher, respectively, in seedlings treated with 0.5% WV and grown in the date palm compost-vermicompost CM, compared to those treated with the same concentration of WV but grown in coco peat-peat moss CM. Additionally, the Fv/Fm ratio saw a 52% increase. When plant nutrition was enhanced with the date palm compost-vermicompost CM and 1% WV, auxin content rose by 130% compared to seedlings grown in coco peat-peat moss CM and treated with 0.5% WV. The study demonstrates that using 0.5% WV in conjunction with date palm compost-vermicompost CM significantly betters the quality of cucumber seedlings, outperforming other treatment combinations.

Investigation of a novel biofilm model close to the original oral microbiome

A more optimized culture medium used in vitro to mimic the bacterial composition of original oral flora as similar as possible remains difficult at present, and the goal of this study is to develop a novel oral biofilm medium to restore the original oral microbiome. Firstly, we conducted a systematic literature review by searching PubMed and summarized the current reported culture media in vitro. Seven culture media were found. We used mixed saliva as the origin of oral species to compare the effects of the above media in culturing oral multispecies biofilms. Results indicated that among the seven media brain heart infusion containing 1% sucrose (BHIs) medium, PG medium, artificial saliva (AS) medium, and SHI medium could obviously gain large oral biofilm in vitro. The nutrients contained in different culture media may be suitable for the growth of different oral bacteria; therefore, we optimized several novel media accordingly. Notably, results of crystal violet staining showed that the biofilm cultured in our modified artificial saliva (MAS) medium had the highest amount of biofilm biomass. 16S rRNA gene sequencing showed that the operational taxonomic units (OTUs) and Shannon index of biofilm cultured in MAS medium were also the highest among all the tested media. More importantly, the 16S rRNA gene sequencing analysis indicated that the biofilm cultured in MAS medium was closer to the original saliva species. Besides, biofilm cultured by MAS was denser and produced more exopolysaccharides. MAS supported stable biofilm formation on different substrata. In conclusion, this study demonstrated a novel MAS medium that could culture oral biofilm in vitro closer to the original oral microbiome, showing a good application prospect.Key points• We compare the effects of different media in culturing oral biofilms• A novel modified artificial saliva (MAS) medium was obtained in our study• The MAS medium could culture biofilm that was closer to oral microbiome

The effects of culture media and media components on the development of rat embryos

After in vitro culture of rat embryos, blastocyst rates are lower than the other species because of the embryonic block observed in the 2- or 4-cell stages in vitro. Optimal culture media and systems that provide variable physiologic needs in the different stages of rat embryos. The modifications of rat embryo culture media could have a positive effect on increasing the blastocyst rates. However, since the results of rat embryo studies are changed depending on factors like strains preferred, maintenance conditions and different commercial products added to the culture media, the success rate of producing healthy newborns for reproductive biotechnological studies has not yet reached the desired level by using current embryo culture media. Understanding the needs of rat embryos cultured from zygote to blastocyst stage in vitro is important for successful advanced studies such as cloning and transgenesis. The purpose of this review is the effects of different culture media and media components on the preimplantation stages of rat embryos and get a perspective for developing the culture media

Effect of Different Culture Media on Growth and Establishment of Phomopsis vexans Inciting Fruit Rot of Brinjal (Solanum melongena L.)

The fruit rot caused by Phomopsis vexans (Sacc. & Syd.) Harter is a destructive disease of brinjal and is considered to be a major constraint for its production. The present study was carried out at the Centre of Excellence for Sanitary and Phytosanitary, Department of Plant Pathology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, for the evaluation of different culture media on growth and development of Phomopsis vexans. The study showed the maximum radial growth on OMA (90.00 mm), followed by PDA (88.20 mm) and Sabouraud Dextrose Agar (80.16 mm). The pycnidial densities were recorded as excellent on OMA and TFA, good on PDA, SDA, BFA, and MEA, and poor on YMA and CMA media with brown to black colour, while on RSA and WA they were absent. The colony colour were observed on several cultural media with circular and irregular shapes. The maximum pycnidial size was recorded on TFA (454.35×316.18 µm), followed by SDA (415.60× 296.26 µm) and BFA (324.72×368.84 µm), whereas the minimum was recorded on CMA (289.48×128.52 µm), followed by MEA (308.66×140.25 µm). The α-conidia were noticed to be sub-cylindrical and elliptical in shape and largest conidia observed on TMA (10.6×2.6µm), followed by BFA (9.8×2.6µm), and the smallest on RSA (6.1×1.6µm). The number of conidia was recorded highest on OMA (29.) medium and lowest on RSA (12.63). The β-conidia were not observed in this study. The number of guttullae were recorded 1-2 in all tested culture media except TFA and SDA, which had 2-3.

Effects of Different Media and Their Strengths in In Vitro Culture of Three Different Cistus creticus L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers

Cistus creticus L. (rockrose), a species of ecological and medicinal significance, constitutes a valuable component of the Mediterranean ecosystem. The present study investigated the effect of the inorganic salt concentration of Murashige and Skoog medium (MS), woody plant medium (WPM), and Driver and Kuniyaki Walnut medium (DKW) at several strengths (1/8×, 1/4×, 1/2×, 1×, and 2×) on the in vitro growth and organogenesis of rockrose. Significant interactions were observed throughout the experiments between pairs of plant origins, medium types, and strengths, and we also examined the extent to which they affected the studied traits was examined. The types of nutrient medium affected all studied traits except shoot and root percentages. The maximum growth percentage (143.49%) was gained using full-strength WPM. The best performance in shoot percentage was obtained using MS (100%) at several strengths along with 1× WPM (100%). The topmost rooting percentage values (98.61%) were obtained using 1× WPM and 1/2× DKW. The highest number of shoots and roots were observed using full-strength MS (9.39) and half-strength WPM (6.49), respectively. The maximum values for shoot and root length were achieved using 1/2× MS (0.78 cm) and 1/8× WPM (1.55 cm), respectively. The origin of the plant material did not influence any studied trait. Moreover, the genetic relations among the populations used in the in vitro culture were assessed using simple sequence repeats (SSR) markers. Twenty-eight alleles were identified across all five STR loci. The different and effective alleles per locus were 5.60 and 4.72, respectively. The average observed and expected heterozygosity was estimated at 0.52 and 0.72, respectively. Shannon’s information index and the inbreeding coefficient (F) were assessed at 1.48 and 0.30, respectively, revealing a narrow genetic base and high genetic similarity among origins, suggesting that they belong to the same population.

Effect of different media on primary hardening of banana tissue culture plants in greenhouse conditions

Effect of different media on primary hardening of banana tissue culture plants in greenhouse conditions

Effect of different media on growth of cucumber under green-house conditions

Effect of different media on growth of cucumber under green-house conditions

Effect of Different culture media, temperature and pH for growth of Rhizoctonia solani causing Sheath Blight Disease in Rice Mat Nursery

Over the last decade Sheath Blight of Rice as a major constant in rice mat nursery production has been identified globally and integrated disease resistance strategy for Sheath Blight of Rice has been primary focus in south east Asia particularly in the kharif and rabi rice growing areas like Bangladesh, Eastern India, Terai region of Nepal and Pakistan. Rhizoctonia solani (Kuhn.) is the causal agent of Sheath Blight Disease in Rice Mat Nursery. It is a soil inhabiting fungal pathogen which favors hot and humid temperature. Keeping in view the consequentiality of the disease due to transmute in climatic conditions, studies were conducted on the cultural and morphological diversity of the pathogen in different media, pH and temperature conditions. This research was conducted at Department of Plant Pathology in Uttar Banga Krishi Viswavidyalaya. The fungus Rhizoctonia solani gives highest mycelial growth on PDA (Potato dextrose Agar) media which is 60.78 mm and it is followed by Glucose peptone Agar media (55.33 mm) and V-8 juice Agar media (51.11 mm) whereas the lowest growth was observed in Czapek Dox Agar media (19.66 mm) followed by Oat meal Agar media (26.66 mm). In different pH, maximum mycelial growth was observed at pH 7 i.e., at neutral pH (62.33 mm) while they showed less growth in too acidic pH i.e., at 4 and at too high pH i.e., at pH 10. In different temperature, the highest mycelial growth was observed at 26⁰C (62.91 mm) to 28⁰C (62.05 mm) i.e., they prefer normal temperature for their growth.

Optimization of moving bed biofilm reactors for the treatment of municipal wastewater

The properties of biocarriers significantly influence the performance of a moving bed-biofilm reactor (MBBR). This study aimed to assess the impact of media type, filling ratio, and hydraulic retention time (HRT) on biofilm formation and MBBR performance in both batch and continuous setups using real municipal wastewater. Two different media, high-density polyethylene (HDPE) and polypropylene (PPE), with varying surface area and properties were used. Biofilm growth and MBBR performance were monitored and optimized using response surface methodology. The effect of different media was investigated for three filling ratios of 20%, 40% and 60% and HRT of 4, 6 and 8 h. Results depicted a better biofilm growth on HDPE media in comparison to PPE carriers due to difference in media structure and surface properties. At all the conditions tested, HDPE media showed comparatively better performance for the removal of organic matter and nutrients than PPE media. The maximum organic matter removal efficiency was found as 77% and 75% at an HRT of 6 h and filling ratio of 40% for HDPE and PPE media, respectively. The ammonia removal was also found better for HDPE media due to its geometry and structure favoring the anoxic conditions with maximum removal of 89% achieved at 6-h HRT and 40% filling ratio. Overall, the system with HDPE media indicated more stability in terms of reactor performance than PPE carriers with variations in the operating conditions.

Influence of Media Information Sources on Vaccine Uptake: The Full and Inconsistent Mediating Role of Vaccine Hesitancy

Vaccine hesitancy is a significant public health concern, with numerous studies demonstrating its negative impact on immunization rates. One factor that can influence vaccine hesitancy is media coverage of vaccination. The media is a significant source of immunization information and can significantly shape people’s attitudes and behaviors toward vaccine uptake. Media influences vaccination positively or negatively. Accurate coverage of the benefits and effectiveness of vaccination can encourage uptake, while coverage of safety concerns or misinformation may increase hesitancy. Our study investigated whether vaccine hesitancy acts as a mediator between information sources and vaccination uptake. We analyzed a cross-sectional online survey by the European Commission of 27,524 citizens from all EU member states between 15 and 29 March 2019. The study used structural equation modeling to conduct a mediation analysis, revealing that the influence of media on vaccine uptake is fully mediated by vaccine hesitancy, except for television, which depicted an inconsistent mediating role. In other words, the effect of different media on vaccine uptake is largely driven by the extent to which individuals are hesitant or resistant to vaccinating. Therefore, media outlets, governments, and public health organizations must work together to promote accurate and reliable information about vaccination and address vaccine hesitancy.

Generations X, Y, Z: Attitudes toward social workers in the age of media technologies

The media portrayal of social workers (SWs) tends toward a negative bias that undermines their public image. The main purpose of the current study was to examine differences between three generational cohorts: Gen X, Gen Y, and Gen Z in terms of the effects of different media as a source of information about SWs on public attitudes toward them. The sample included 783 respondents. The findings reveal dissimilar associations between exposure to different media as information sources about SWs and public attitudes toward them among three generations. In Gen X, attitudes toward SWs were negatively associated with exposure to print newspaper references and positively associated with exposure to radio. In Gen Y, attitudes toward SWs were negatively associated with electronic media reportage and positively associated with entertainment media and TV news references. No association was found between media exposure as an information source about SWs and attitudes toward them in Gen Z. Also, a negative association was found between the perception that the media depict SWs negatively and attitudes toward SWs among all generations. Substantial cooperation between SWs and media representatives is required to promote a more balanced media portrayal of SWs and a more positive attitude toward them.

Abstract 24 Development of an In Vitro B-Cell Differentiation Model for the Characterization of the Origin and Causation of ETV6::RUNX1+ Preleukemic Cells

Abstract Introduction Pregnant women are exposed to various carcinogenic substances that can subsequently affect their progeny. Recurrent preleukemic chromosomal translocations characteristic for childhood acute lymphoblastic leukemia (ALL) frequently emerge before birth in utero. The most common translocation t (12;21) occurs in 25% of B-cell precursor ALL and results in the formation of the chimeric oncogenic transcription factor ETV6::RUNX1. Objectives We aim to analyze factors promoting the generation of preleukemic ETV6::RUNX1 translocations, investigate the biological origin of these preleukemic cells, and develop an in vitro model of early B-cell differentiation to analyze the mechanisms underlying the persistence of ETV6::RUNX1+ preleukemic cells and leukemia evolution in children. Methods Genomic inverse PCR for exploration of ligated breakpoints (GIPFEL) (Schafer et. al., 2018) was applied to identify ETV6::RUNX1+ cord blood units (CBU). Screening results were validated by Sanger sequencing and quantified by real-time PCR (ipsogen ETV6-RUNX1 Kit, Qiagen). ETV6::RUNX1+ CBUs were thawed, sorted for CD34+ and CD19+ cells, and analyzed by qPCR. CD34+ were differentiated towards B-cell lineage on MSC feeder lines according to a modified protocol (Ichii et. al., 2008). Results We screened a cohort of 1405 cord bloods collected in 2004 and discovered that 103 (7.3%) carried the ETV6::RUNX1 fusion gene. The preleukemic translocation was detected in CD34+ stem and early B progenitor cells. For the establishment of an in vitro B-cell differentiation model, bone marrow (BM-MSCs) and CB (USSCs, CB-MSCs) stromal feeder cells were compared and the effect of different media (SCGM, DMEM/5 % FCS, MSCBM/10 % FCS) in the presence of stem cell factor (SCF), Flt-3, IL3, +/- IL7 was assessed. BM-MSCs demonstrated the highest ability to support B-lymphopoieses towards CD19+ cell differentiation in MSCBM media (~30%), primarily due to the release of IL-7 by the feeder layer. Currently, factors that may promote generation of preleukemic translocations in utero e.g. nitrosamines contained in food products, are being tested in this model. Discussion We identified the HSC/early B progenitor state as the origin of ETV6::RUNX1 translocations in CB. The establishment of an in vitro B-cell differentiation system will allow to test the impact of environmental carcinogens on the generation of ETV6::RUNX1+ pre-leukemic cells and their leukemic evolution.