Effect of Different culture media, temperature and pH for growth of Rhizoctonia solani causing Sheath Blight Disease in Rice Mat Nursery

Abstract

Over the last decade Sheath Blight of Rice as a major constant in rice mat nursery production has been identified globally and integrated disease resistance strategy for Sheath Blight of Rice has been primary focus in south east Asia particularly in the kharif and rabi rice growing areas like Bangladesh, Eastern India, Terai region of Nepal and Pakistan. Rhizoctonia solani (Kuhn.) is the causal agent of Sheath Blight Disease in Rice Mat Nursery. It is a soil inhabiting fungal pathogen which favors hot and humid temperature. Keeping in view the consequentiality of the disease due to transmute in climatic conditions, studies were conducted on the cultural and morphological diversity of the pathogen in different media, pH and temperature conditions. This research was conducted at Department of Plant Pathology in Uttar Banga Krishi Viswavidyalaya. The fungus Rhizoctonia solani gives highest mycelial growth on PDA (Potato dextrose Agar) media which is 60.78 mm and it is followed by Glucose peptone Agar media (55.33 mm) and V-8 juice Agar media (51.11 mm) whereas the lowest growth was observed in Czapek Dox Agar media (19.66 mm) followed by Oat meal Agar media (26.66 mm). In different pH, maximum mycelial growth was observed at pH 7 i.e., at neutral pH (62.33 mm) while they showed less growth in too acidic pH i.e., at 4 and at too high pH i.e., at pH 10. In different temperature, the highest mycelial growth was observed at 26⁰C (62.91 mm) to 28⁰C (62.05 mm) i.e., they prefer normal temperature for their growth.