Abstract
BACKGROUND: Hyaline cartilage is an avascular tissue that covers surface of large joints. This tissue, with a small number of cells and a high content of extracellular matrix proteins, has limited regenerative capacity. Recovery the articular cartilage remains a relevant and not yet fully resolved issue today. A promising approach is the use of biomedical products containing modified cells. AIM: To investigate the effects of different culture media on the chondrogenic modification of cells and to compare the results. METHODS: Human dermal fibroblasts and rabbit mesenchymal stem cells were modified using chondrogenic differentiation medium StemPro or recombinant TGF-β3 protein. Changes in the expression of genes responsible for chondrogenesis (Acan, Tgfβ3, Col2α1, Comp) were measured using the Real-Time PCR method -2ΔΔCt. RESULTS: It was shown that human dermal fibroblasts are capable of chondrogenic modification using protein media. This cell type is easily accessible for harvesting, does not require special conditions for cultivation, is easily scalable, and can be used for allogeneic transplantation. CONCLUSION: The obtained data can be used in the design of tissue engineering products for the regeneration of hyaline cartilage based on allogeneic dermal fibroblasts.
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