Effects Of Different Cultivation Methods Research Articles

Cropping with Vicia villosa and native grass improves soil's bacterial structure and ecological network in a jujube orchard.

In a jujube orchard, cropping withgrass may influence bacterial diversity and ecological networks due to changes of physicochemical properties in soil, which has a serious effect on the stability of soil ecosystems. The aim of this study was to analyze the effects of different cultivation methods (CK: cleaning tillage; NG: cropping with native grass; VV: cropping with Vicia villosa) on the soil's bacterial structure and its co-occurrence network in a jujube orchard. The results showed that the highest moisture content, total nitrogen, and organic matter in the rhizosphere soil of a jujube orchard was found in the VV group. The soil's moisture content, total nitrogen, and organic matter in the VV group were 2.66%, 0.87 gkg-1, and 5.55 mgkg-1 higher than that found in the CK group. Compared to the CK group, the number of unique species in the rhizosphere soil in the NG and the VV groups increased by 7.33% and 21.44%. The PICRUSt and FAPROTAX analysis showed that sown grass had a greater influence on the ecological function of the soil's bacteria. Cropping with Vicia villosa and native grass significantly increased aerobic chemoheterotrophy, nitrogen respiration, nitrate reduction related to biochemical cycles, and the relative abundance of genes related to carbohydrate metabolism and the biodegradation of xenobiotics. The bacterial network complexity in the NG group was higher than that in the CK and VV groups and was greatest in the hub nodes (OTU42, Bacteroidota; OTU541, Nitrospiraceae). In this study, the ecological benefit seen in the soil's microbial function provides support to the theory that cropping with grass (Vicia villosa) increases the sustainable development of a jujube orchard.

Metabolomics-Based Analysis of the Effects of Different Cultivation Strategies on Metabolites of Dendrobium officinale Kimura et Migo.

Dendrobium officinale Kimura et Migo is a famous plant with a high medicinal value which has been recorded in the Chinese Pharmacopoeia (2020 Edition). The medicinal properties of D. officinale are based on its chemical composition. However, there are no reports on how different cultivation methods affect its chemical composition. In order to reveal this issue, samples of the D. officinale were collected in this study through tree epiphytic cultivation, stone epiphytic cultivation, and greenhouse cultivation. Polysaccharides were determined by phenol sulfuric acid method and secondary metabolites were detected by the UPLC-MS technique. In addition, with regards to metabolomics, we used multivariate analyses including principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA) to screen for differential metabolites which met the conditions of variable importance projection values >1, fold change >4, and p < 0.05. The differential metabolites were taken further for metabolic pathway enrichment analysis, which was based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and validated by antioxidant activity. Comparing the three groups of samples according to the standards of the ChP (2020 edition), the results showed that the polysaccharide content of the samples from stony epiphytic cultivation and greenhouse cultivation was significantly higher than that of the samples from live tree epiphytic cultivation. Metabolomic analysis revealed that there were 185 differential metabolites among the 3 cultivation methods, with 99 of the differential metabolites being highest in the stone epiphytic cultivation. The results of the metabolic pathway enrichment analysis showed that the different cultivation strategies mainly effected four carbohydrate metabolic pathways, five secondary metabolite synthesis pathways, six amino acid metabolic pathways, one nucleotide metabolism pathway, three cofactor and vitamin metabolism pathways, and one translation pathway in genetic information processing. Furthermore, D. officinale from stone epiphytic cultivation which had the best antioxidant activity was implicated in differential metabolite production. This study revealed the effects of different cultivation methods on the chemical composition of D. officinale and also provided a reference for establishing the quality control standards to aid its development and utilization.

Identification of Phenotypic Traits Associated with Tuber Yield Performance in Non-Staking Cultivation of Water Yam (Dioscorea alata L.)

For sustainable food production, labor-saving cultivation systems are required. Yam, a major food crop, is cultivated mostly with supports such as stakes (staking), which increases tuber yield (TY) but involves high material and labor costs. We, therefore, focused on non-staking water yam cultivation, in which no stakes are used. The effects of different cultivation methods (staking vs. non-staking) on TY, yield components, shoot traits, and tuber shape of six cultivars were investigated in a two-year field experiment, and phenotypic traits related to yielding ability in non-staking cultivation were analyzed. Averaged across years and cultivars, TY was significantly lower (by 19%) in non-staking than in staking cultivation because of smaller single-tuber weight. TY was significantly affected by the cultivation × cultivar interaction. We found no difference among cultivars in staking cultivation. In non-staking cultivation, Yamatomakousha and Shirokoushaman 1 cultivars had higher TY than the other cultivars, which was similar to their TY in staking cultivation. Shoot dry weight and vine number were closely associated with TY in both cultivations, whereas lower tuber length-to-width ratio was strongly related to higher TY only in non-staking. Tubers of Yamatomakousha and Shirokoushaman 1 were more rounded than those of other cultivars. In non-staking cultivation, these two cultivars showed a higher vine number and, thus, maintained higher TY owing to higher above-ground growth. Therefore, rounded tubers and high vine number are target traits for non-staking cultivation of water yam.

Enhancing Production Through Input Use Efficiency of Virus-Resistant Pune Selection-3 Papaya Variety

An experiment was carried out using virus-resistant papaya variety Pune Selection-3 to study the effect of different cultivation methods for enhanced production and input use efficiency at Horticultural Research Farm, RAU, Pusa during the year 2016-2019. The outcomes of raised bed cultivation (a), drip irrigation (b), fertigation (c), polyethylene mulching (d) along with micronutrient spray (e) were analyzed using different combinations of these parameters as treatments for growth, yield-related parameters, and fruit quality. Nutrient use efficiency was also assessed by examining various interactive components such as soil nutrient content, nutrient acquisition with nutrient content in the leaves, the efficiency of nutrient utilization informs of the biomass produced, fruit yield, and fruit nutritional quality. The plant height was maximum in T1 (155 cm) which was significantly higher than the plant of control treatment. The flowering time was found to be reduced in all treatments. However, total soluble sugar was higher in T3. Overall, when the B:C ratio was compared, the treatment (T1) showed a higher value along with a 2.38-fold fruit yield/plant and 0.89-fold increase in fruit yield per hectare. The nutritional quality of fruits enhanced in T1 treated plants having a 1.78-fold increment in flavonoid content, and 1.2-fold more lycopene. The phenolic content was also 1.8-fold higher than the control (T5). The result obtained in the present study demonstrates that with the implementation of a suitable fertigation system in papaya cultivation we can obtain papaya growth, fruit yield per plant, and fruit quality by targeting enhancement in cost: benefit ratio.&#x0D; Int. J. Appl. Sci. Biotechnol. Vol 10(2): 84-92.

Effect of a Ridge-Furrow Mulching System and Limited Supplementary Irrigation on N2O Emission Characteristics and Grain Yield of Winter Wheat (Triticum aestivum L.) Fields under Dryland Conditions

Knowledge of the characteristics of N2O emissions and the influential mechanism is of great significance to mitigate greenhouse gas emissions in semi-arid areas. In the present study, a three-year water-control study was conducted; three simulated rainfall amounts (heavy, normal, and light rainfall = 275, 200, and 125 mm, respectively), two wheat (Triticum aestivum L.) planting modes (RF (ridge–furrow mulching system) and TF (traditional flat planting)) and four supplementary irrigation amounts (150, 75, 37.5, and 0 mm) were set up. The effects of different cultivation methods and irrigation amounts on soil N2O emissions, the soil water content, available nitrogen content, and denitrifying enzyme activity were investigated to clarify the N2O emission mechanism in winter wheat fields (Triticum aestivum L.). The results obtained after three years showed that compared with TF, the N2O emissions under RF decreased by 21.62–30.72% (p &lt; 0.001), whereas the soil water content increased by 6.26–8.82%, the available nitrogen content decreased by 1.71–16.24%, and the denitrifying enzyme activities increased by 0.2–24.16% under heavy rainfall conditions. Under conditions with normal and light rainfall, the N2O emission fluxes under RF increased by 3.66–12.46% and 6.08–15.57% (p &gt; 0.05), while the soil water contents increased by 6.13–11.49% and 8.05–13.88%, the soil available nitrogen contents decreased by 11.0–21.42% and 19.93–34.44%, and the denitrifying enzyme activities increased by 0.01–24.08% and 0.03–20.79% compared with TF. Principal component analysis showed that the main factors related to N2O emissions under RF were the soil moisture content and available nitrogen content; these factors combined explained 94.37% the variation of the N2O emissions. However, the main factors under TF were the soil moisture content and denitrifying enzyme activity; these factors combined explained 85.81%. In the heavy and normal rainfall years, compared with TF, using RF and 75 mm irrigation achieved the goal of reducing water usage as well as decreasing the N2O emissions (or N2O increase was not significant). In light rainfall years, RF with 150 mm irrigation obtained significant reductions in water usage compared with TF but it also increased the N2O emission flux. Under different rainfall years, the yield of RF increased by 2.89–50.44% compared with the TF system, and the increase in wheat grain yield increased with decreasing rainfall.

Comparison of changes in fecal microbiota of calves with and without dam.

In pastoral areas and semi-agricultural and semi-pastoral areas of Sichuan, beef cattle breeding mode is mainly dependent on nature to raise livestock. On the one hand, owing to the shortage of forage grass in spring, cows suffer from malnutrition. On the other hand, competition for milk between human and livestock further deepens the malnutrition of newborn calves, and the mortality rate even exceeds 40%, resulting in serious waste of beef cattle source resources. The objective of this study was to investigate the effect of different cultivation methods (calves with and without dam) and age on calves hindgut microbiome. Sixteen healthy calves (Yak ♂ × Pian cattle ♀, with similar birthday 0 ± 2 d and body weight 13.1 ± 1.13 kg), were selected and randomly divided into two groups. The control group was cultivated with heifers, whereas the treatment group was cultivated without heifers and was fed milk replacer during the whole 95 days formal experimental period. Fecal samples were collected on 35, 65 and 95 days of age for high-throughput sequencing. The α-diversity was different between the two groups on day 35; however, the bacterial species richness and diversity was almost not different on day 95. Principal coordinates analysis revealed significant difference between the two groups on all the three time points, and the timepoints of day 65 and 95 were closer and separated from the timepoints of day 35 in calves with dam, whereas the timepoints of day 35 and 65 were closer and separated from day 95 in calves without dam. As time passed, the abundance of Firmicutes increased, while Proteobacteria and Actinobacteria decreased in calves with dam. But in calves without dam, the abundance of Bacteroidetes and Proteobacteria increased on day 65 and then decreased on day 95. In genus level, the relative abundance of Bacteroides decreased in calf with dam while its abundance increased first and then decreased in calf without dam but both resulted in the range of 3.5~4.5%. The relative abundance of Lactobacillus decreased, whereas Ruminococcaceae UCG-005 increased in both groups as the calf grew up. It was concluded that the richness and evenness of the microbial communities was higher in calves with dam than without dam, and a stable gut microbiome in calve with dam is established earlier than calf without dam.

The effect of different cultivation methods on some chemical soil properties and the presence of the residues of alhagi and fungi

A field experiment was carried out at the research station / Agriculture College / Al-Muthanna University in the spring season 2016 to study the effect of water rationing and various agricultural methods and add the debris of the herbs, fungi, Rhizoctonia solani and the penicillium chrysogenum on some soil chemical properties. Results showed that the highest value of Total dissolved salt (TDS) was in the method of agriculture with tunnel + without debris + R.s where it reached 5.53 ppm while the lowest value in the normal method of agriculture + Con + Convalescent at 1.58 ppm. The effect on salinity of the soil showed that the highest value of electrical conductivity in the treatment was tunnel + without debris + R.s, reaching 10.8 Diszymes. M-1 and the lowest salinity in the treatment normal + Convolution Con + reached 3.53 Disimens. The results showed that the highest pH was in the normal treatment + R.s + at 8.50, while the pH in the bags was less than + R . s where it was 6.25.

Establishing healthy seedlings of Enhalus acoroides for the tropical seagrass restoration

Enhalus acoroides, the dominant species in tropical seagrass meadows, is experiencing declines worldwide for complicated reasons and the restoration of these meadows is extremely urgent. Nursery stock grown from the initial seedlings could be used to enhance success of E. acoroides meadow restoration. In this study, the effects of different cultivation methods on the seedling development and longer-term cultivation of E. acoroides were compared using various artificial culture substrates (culturing with sea mud substrate, agar substrate, without a matrix, and using a submerged foam substrate). Results suggested that none of the seedlings showed any sign of root gemination when cultured with sea mud substrate. Though the seedlings cultured with an agar substrate grew faster than those cultured with sea mud, those seedlings could not be cultured further as the agar substrate softened and became rotten after 3 weeks. The initial seedlings cultured in matrix-free seawater germinated with normal leaf growth but no roots developed. In contrast, the initial seedlings planted in holes of a submerged foam substrate grew successfully, developing into healthy seedlings with green leaves and long roots. These seedlings could be cultured for up to 23 weeks. Based on these results, a new, low-cost and labor-efficient method for E. acoroides seedling development was established, which might have a great application potential for efficient E. acoroides seagrass meadows restoration.

The contents of ganoderic acids in mycellium of different Ganoderma species (Ganodermataceae) obtained by different methods of cultivation

The effect of different cultivation methods on the content of ganoderic acids of 7 species, 10 strains of the Ganoderma genus (Ganoderma applanatum 1899; Ganoderma сarnosum 2502; Ganoderma lucidum 1904; Ganoderma resinaceum 2477, 2503; Ganoderma sinense 2516; Ganoderma tsugae 1848, 2024, 2566, Ganoderma oregonense 2560) genus fungi from the IBK Mushroom Culture Collection M.G. Kholodny Institute of Botany of National Academy of Sciences of Ukraine was investigated. It has been shown that the submerged cultivation method is more efficient for the accumulation of ganoderic acids for five strains. In the mycelium of the strain G. sinense 2516 was the highest content of ganoderic acids – 25.2 ± 1.5 mg / g. The productivity (yeld) of ganoderic acids synthesis is much higher with using the submerged culture cultivation method for mycelium of all used species and strains since the use of this method provides the accumulation of much more biomass in comparison with the static liquid cultivation method. The highest yield amount of ganoderic acids was in the mycelium of the G. tsugae 2024 and G. tsugae 2566 species, namely: 0.35 ± 0.019 and 0.36 ± 0.028 g / l. It was proved that the modified extraction method significantly reduces the extraction time of ganoderic acids. Extraction time is reduced from 14 to 2 days. For the G. sinense 2516 and G. tsugae 2024 strains was determined content of the ganoderic acids and their yield in dynamics of grows in the submerged culture on 6, 8, 10, 12, 14, 16, 18 and 20 day of cultivation. The highest amount of the ganoderic acids content was accumulated by the mycelium of the strain G. sinense 2516 – it was 26.4 ± 1.5 mg / g on the 14th day of cultivation. The highest yield of the ganoderic acids was in G. sinense 2516 on 14th day, and G. tsugae 2024 mycelium on the 16th day of cultivation with the next numbers 0.6 ± 0.031, 0.62 ± 0.033 and 0.62 ± 0.027 g/l.

The Effect of Cultivation Method of Strawberry (Fragaria x ananassa Duch.) cv. Honeoye on Structure and Degradation Dynamics of Pectin during Cold Storage.

The high quality and long shelf life of strawberry fruit are largely dependent on the cultivation method. The goal of this experiment was to study the effect of different cultivation methods on molecular structure and rheological properties of pectin extracted from strawberry quality parameters during cold storage. Three methods of cultivation of strawberry cv. Honeoye were tested: organic cultivation on raised beds, organic cultivation with the flat-planted method and conventional cultivation with the flat-planted method. The nanostructure of pectin (AFM), its chemical structure (FT-IR) and rheological properties were studied. The fruits were also tested by size, dry matter, firmness, acidity and the content of soluble solids, anthocyanin, phenolics, vitamin C and galacturonic acid. Pectin isolated from organic strawberries was more rapidly degraded than conventional strawberry pectin, which limits the possibilities for their processing and use as gelling or stabilizing agents at 20 °C. The differences in fruit quality were particularly noticeable with respect to the anthocyanin content, which was significantly higher for organic strawberry. The organic fruit also had better sensory properties because of its lower acidity and higher soluble solid content (SSC). These and other results from this experiment showed that strawberries produced by organic farming methods had better biochemical properties compared to conventional fruit; however, pectin transformation undergone faster limits their further technological applications.

The Effects of Fertilizer Treatment, Rhizome Seed Size, and Day of Harvest In Java Turmeric (Curcuma xanthorrhiza Roxb.)

Java turmeric (Curcuma xanthorrhiza Roxb.) is cultivated as a secondary crop, resulting in variable rhizome quality which can be increased by suitable cultivation methods. This study investigated the effect of different cultivation methods on the rhizome yield of Java turmeric. Different fertilizer treatments(none, organic, inorganic, and semi-organic fertilizer), three groups of rhizome seed size (small (50–80 g), medium (100–150 g), and large (200–250 g)) and three groups of harvesting age (eight, ten, and twelve months after planting) were evaluated in a split plot design experiment. Results show that large rhizome seed size together with organic fertilizer treatment increased secondary rhizome production, yielding the highest number, weight and diameter. As high levels of starch in the primary rhizome are crucial for growth of the plant, the use of large rhizomes for propagation is indicated in Java turmeric cultivation. The highest weight and number of primary rhizomes were yielded when plants were harvested twelve months after planting.

Effects of Different Cultivation Methods on Yield of Purple Yam

Purple yam (Dioscorea alata L.) belonging to the herbaceous vine plants, dioscorea genus, Dioscorea subject and one kinds of edible plants. It’s Native to tropical Asia, purple yam is plants grown under a suitable short sunshine growth environment, is a kind of thermophilic plants, mainly temperate zone in China's southern provinces. We performed different cultivation methods on Yield and variety comparison test for planting on north area of China. To choicing the different position of the yam block, shuch as the top and the middle block and different weight of harvested. Then average per mu 2600 strains were calculated and counting the per mu yield. The growth of the top position of the yam is better than the middle of plant growth. The yield of the top position is higher than the same weight of tubers of the middle position. The best kind of potato weight should be between 100-150g, so that it can reduce costs, but also have a considerable income.

Effects of Different Cultivation Methods on the Growth Characteristics, Physiochemical and Functional Materials in Pigmented Rice Varieties CheongPungHeuk-Chal and CheongPungHeukHyang-Chal

Effects of Different Cultivation Methods on the Growth Characteristics, Physiochemical and Functional Materials in Pigmented Rice Varieties CheongPungHeuk-Chal and CheongPungHeukHyang-Chal

Effects of Different Cultivation Methods on Growth Stage, Dry Matter Accumulation, and Yield of Early Rice in Double Cropping Rice Fields

Effects of Different Cultivation Methods on Growth Stage, Dry Matter Accumulation, and Yield of Early Rice in Double Cropping Rice Fields

Effects of Different Cultivation Methods and Straw Incorporation on Grain Yield and Nutrition Quality of Rice

Plastic film mulching cultivation (PFMC) under non-flooded condition has been considered as a new water-saving technique in rice production, while yield decline from continuous cropping of aerobic rice is a constraint to the widespread adoption of PFMC. Rice straw incorporation has been proposed to counter this negative effect in recent decades. This study examined the effects of three cultivation methods and rice straw incorporation on rice grain yield and quality using Liangyoupeijiu (an indica hybrid). The three cultivation treatments were: conventional flooding cultivation (CFC); non-flooded plastic film mulching cultivation (PFMC); no mulching cultivation in non-flooded condition (NMC). Compared with that under CFC, average rice grain yield under PFMC from 2008 to 2010 was significantly improved by 8.0%, while total amino acids content was decreased by 3.5%, no obvious effect was observed on rice protein, Fe and Zn concentrations in rice; under NMC, the reduction in yield, protein, total amino acids and Fe concentrations were 5.1%, 4.4%, 9.3%, and 11.8%, respectively. With rice straw incorporation, grain yield was improved by 113.6, 142.6, and 522.1 kg ha 1 under CFC, NMC, and PFMC, respectively. Average rice grain yield, Fe and Zn contents in brown rice were significantly improved by 3.3%, 3.1%, and 6.4% with rice straw incorporation. The results of the three years showed the same trend in rice grain yield and nutrition quality. The results indicated that PFMC could improve not only grain yield, but also part of rice nutrition quality. Rice straw incorporation could significantly improved rice grain yield as well as Fe and Zn concentrations in brown rice, which would be a good method to overcome grain yield decline under long term PFMC.

Effect of different cultivation methods on germination and growth of Puccinellia tenuiflora

The effects of drip irrigation and different cultivation methods on seed germination and growth of Puccinellia tenuiflora in saline-sodic soils were investigated in field experiments during 2008-2010 in the Songnen Plain, China. Drip irrigation was applied to waste saline-sodic P. tenuiflora grasslands for both flat and ridge cultivation methods. Drip irrigation affected the distributions of electrical conductivity of saturated-soil extract (ECe) and pH of the saline-sodic soil. After cropping under drip irrigation for two years, the ECe and pH had decreased greatly in the root zone on both the flat and ridge lands, and enabled P. tenuiflora seeds to germinate and grow rapidly. In addition, the plant height, number and length of spikes, coverage and aboveground biomass were all higher on the flat than on ridge land, but the differences of the two cultivation methods were not obvious. Compared to ridge planting, the flat planting method was more convenient, and flat cultivation and drip irrigation may be appropriate for restoration of saline-sodic grasslands.

Effects of Different Organic Farming Methods on the Concentration of Phenolic Compounds in Sea Buckthorn Leaves

The effects of different cultivation methods on the amount of phenolic compounds in leaves of 1-year-old seedlings of two Finnish sea buckthorn (Hippophae rhamnoides L. ssp. rhamnoides) cultivars 'Terhi' and 'Tytti' were studied in a field experiment established at coastal area in Merikarvia, western Finland. The cultivation methods included different fertilizers (suitable for organic cultivation), mulches (organic and plastic), and land contours (flat vs low hill surface). Two experiments were conducted. The first allowed the estimation of the effects of cultivar, fertilizer, surface contour, and all their interactions, while the other allowed the estimation of the effects of mulches, land contours, and their interactions for the cultivar 'Tytti'. Eleven different hydrolyzable tannins, pentagalloylglucose, and 14 other phenolic compounds were detected by chemical analysis with high-performance liquid chromatography (HPLC). The amount of phenolic compounds varied between different land contours and mulches. The concentrations of gallic acid, pentagalloylglucose, quercetin-3-rhamnoside, monocoumaroyl astragalin A, total hydrolyzable tannins, and condensed tannins were significantly higher on the flat surface than on the low hill surface. The plastic mulch decreased the concentration of gallic acid, hydrolyzable tannins, and condensed tannins compared to the other mulches used. These results suggest ways to cultivate sea buckthorn to produce large amounts of valuable chemicals, especially tannins in the leaves.

The Effect of Genotype and Cultivation Method on the Total Salicylate Yield of Dark-Leaved Willows (Salix myrsinifolia)

Ten clones of dark-leaved willows ( Salix myrsinifolia Salisb.) were grown for two years on two different sites, Luikonlahti and Punkaharju, using various combinations of soil cultivation, fertilization and mulch treatments, with the objective of comparing the effect of different cultivation methods on the growth and total salicylate yield. In cultivated soil, the total salicylate yield ranged from 20 to 220 kg/ha, depending on the clone and cultivation method. The use of black polythene mulch and the addition of mineral fertilizer reduced the total salicylate concentrations of several clones. On the other hand, polythene mulch noticeably increased the hectare yield of total salicylates by promoting biomass accumulation.

Properties of compacted fine-textured soils as affected by crop rotation and reduced tillage

The effects of crop rotation and reduced tillage on physical properties of compacted soil were investigated in field experiments on two fine-textured soils. Field experiments were established in 1990 when trial plots were compacted to 0.45–0.50 m depth by three passes with high axle load traffic. Control plots were not loaded. Both compacted (CO) and control (NC) plots were then ploughed to 0.18–0.23 m depth. In 1991, four cultivation treatments were commenced: RTSC, reduced tillage including autumn stubble cultivation, spring cereals; RTCR same as RTSC but with crop rotation; CTSC, conventional tillage including autumn ploughing, spring cereals; CTCR same as CTSC but with crop rotation. Several soil physical properties were measured in autumn 1994 (silt, Eutric Cambisol) and 1995 (clay loam, Vertic Cambisol), and structure of the soils was visually examined to 0.5 m depth. Soil penetrometer resistance was greater in CO than NC plots in the 0.35–0.49 m layer of the clay loam and the 0.25–0.35 m layer of the silt soil. The subsoil structure was also more massive and homogeneous in CO than in NC plots. The number of cylindrical pores (>2 mm diameter, NB) was nevertheless greater in CO than in NC plots at 0.55 m depth. At the bottom of the topsoil layer, NB was clearly less in conventional tillage (ploughed) than in reduced tillage (stubble cultivated) plots. Likewise, in the layer of 0.15 m below the topsoil, saturated hydraulic conductivity ( K sat) and at 0.55 m depth NB were greater in reduced tillage plots. The K sat and macroporosity (diameter >250 μm) were increased in the upper part of the subsoil by crop rotation with perennial crops. More detailed information about the size distribution of macroporosity and cylindrical pores and the continuity of the pores is required to evaluate the effects of different cultivation methods on the recovery of compacted soil structure.

Biosynthesis of protein by microscopic fungi in solid state fermentation. III. The effect of different cultivation methods and various media on protein biosynthesis by Aspergillus oryzae A. or. 11

AbstractThe effect of the culture method in solid medium and the influence of starch‐containing raw materials on the yield of fungal protein biosynthesis were studied. Three procedures (laboratory bioreactor method, tray method and pile method) gave satisfactory results. Protein yields amounted to 5.0–5.9 g/100 g of starting medium d.m. upon utilization of 20.9–24.1 g carbohydrates. The procedure involving the use of a fermenter with a mixer afforded protein yields by about 50% lower as compared with the three above‐mentioned procedures, and therefore it requires technical improvement.As a result of fungal culture using various starch‐containing raw materials, the protein content in post‐culture products increased by 46–88%, as compared with starting medium. The contents of 13 amino acids (including some exogenous ones which increased by 52–82%) in post‐culture products substantially rose (by 34–63%). The post‐culture products exhibited proteolytic activity (1260–3500 HU/g of d.m.).The kind of the source of starch evidently influenced protein biosynthesis. Media based on potatoes afforded the greatest increases in protein (5.8–5.9 g/100 g of starting medium d.m.), and those containing coarse rye meal and milling by‐products — the smallest ones (4.5–4.6 g/100 g of starting medium d.m.).