It is not yet clear to what extent the physiological regulatory mechanisms that maintain core body temperature are reflected by changes in resting energy expenditure (REE). Particularly in indirect calorimetry with a canopy, the effects of short-term temperature exposures have not yet been investigated. This can be of relevance for the determination of REE in practice. This randomized crossover-study investigated the influence of a 30-minute exposure to 18°C (cool room temperature), 22°C (room temperature), 28°C (thermoneutral zone) and 38°C (heat) on REE determined by assessing the inhaled oxygen volume and the exhaled carbon dioxide volume via indirect calorimetry on four consecutive days. 32 metabolically healthy participants (16 male and 16 female, age: 25 ± 3 years, body mass index: 22.4 ± 1.6 kg/m2) were included in the study after screening examination. Lean body mass, ambient temperature and heart rate were the most important determinants (all P<0.001), and explained 61.3% of the variance in REE. A multivariate linear mixed model analysis revealed that lean mass (15.87 ± 3.66, P<0.001) and ambient temperature (P=0.001) significantly influenced REE. REE significantly differed between 18°C and 28°C (18°C: +96 ± 24 kcal/24 h, P<0.001), 22°C and 28°C (22°C: +73 ± 24 kcal/24 h, P=0.003) and 18°C and 38°C (18°C: +57 ± 23 kcal/24 h, P=0.016). Effects of ambient temperature on REE, especially cold, were detectable after only brief exposure, emphasizing the importance of performing indirect calorimetry with a canopy under controlled environmental conditions. The study was registered with the database "ClinicalTrials.gov" (NCT05505240).Web-Link: Influence of Ambient Temperature on Resting Energy Expenditure of Healthy Adults - Full Text View - ClinicalTrials.gov.
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