A multiscale simulation, data assimilation, forecasting system was developed in support of the SPURS-2 (Salinity Processes in the Upper-ocean Regional Study 2) field campaign. Before the field campaign, a multiyear simulation was produced for characterizing variabilities in upper-ocean salinity, eddy activity, and other parameters and for illustrating major processes that control the region’s upper-ocean salinity at different spatial and temporal scales. This simulation assisted in formulating sampling plans. During the field experiment, the system integrated SPURS-2 measurements with those from routine operational observing networks, including Argo floats and satellite surface temperatures, salinities, and heights, and provided real-time skillful daily forecasts of ocean conditions. Forecast reports were prepared to summarize oceanic conditions and multiscale features and were delivered to the SPURS-2 chief scientist and other SPURS-2 investigators through the SPURS-2 Information System. After the field experiment, the data assimilation system was used to produce a reanalysis product to help quantify contributions of different processes to salinity variability in the region.