Eclipta Alba Research Articles

Assessment of in vitro Anti-inflammatory and Anti-acne-inducing Bacterial Activities of Kameng (Eclipta prostrata Linn.) Cultivated in Suphan Buri Province, Thailand

This research investigates extracts from Kameng (Eclipta prostrata Linn.), originally employed in traditional medicinal recipes, with the aim of developing an extract that can serve as an alternative to antibiotics. For this purpose, the anti-inflammatory and anti-acne-inducing bacterial activities of Kameng crude ethanol extract, grown in Suphan Buri Province were investigated, with anti-inflammatory properties assessed by the inhibition of protein degradation and proteinase inhibitory assays. Findings indicated the potential of Kameng extracts as anti-inflammatory drugs that reduce protein degradation and proteinase activities. Kameng extract concentrations ranging from 0.4 to 4.0 x 104 ppm inhibited protein degradation and proteinase activity by 14.66%-50.75% and 28.57%-72.08%, respectively. The agar disc diffusion method revealed that Kameng extracts had strong inhibitory effects against S. aureus and C. acnes, with inhibition zone diameters of 31.15±0.47 and 37.28±0.47 mm, respectively. The minimal inhibitory concentration (MIC) of Kameng extract against S. aureus and C. acnes was 5.0 mg/mL and 2.5 mg/mL while minimal bactericidal concentration (MBC) of Kameng extract against S. aureus and C. acnes was 20.0 mg/mL and 10.0 mg/mL, respectively. Results suggested the potential of Kameng extract to cure acne and skin inflammation as a herbal cosmetic product.

THERAPEUTIC USE OF BHRINGRAJ IN PANDU: A LITERARY REVIEW IN AYURVEDIC TEXTS

In modern medicine, Pandu, also referred to as Anaemia, is a disorder marked by low haemoglobin or red blood cell counts, which can cause symptoms like weakness, exhaustion, and pale complexion. In the ancient science of Ayurveda, Pandu is considered a disorder primarily affecting the blood tissue (Rakta Dhatu), with Pitta and Kapha Doshas playing significant roles in its aetiology. Bhringraj (Eclipta alba), a revered herb in Ayurveda, has been traditionally utilised for its therapeutic potential in managing various health ailments, including Pandu. This article aims to delve into the therapeutic use of Bhringraj in managing Pandu, drawing insights from different Ayurvedic texts.

Assessment of hemostatic ability of biomaterial based on chitosan and Eclipta prostrata L. extract

The biomaterials based on chitosan and Eclipta prostrata L. extract have been prepared by microemulsion method and solution method (with and without sodium tripolyphosphate (STPP) as a cross-linking agent). The main component in Eclipta prostrata L. extract is flavonoid groups. The structure of the chitosan/extract biomaterials was studied by infrared spectroscopy. The chitosan/extract biomaterial using STPP cross-linker appeared an absorption band at 1152 cm−1 attributed to the vibrations of C–O–P bonds, which proved that chitosan has crosslinked with STPP. The morphology of the biomaterials was investigated by the dynamic light scattering technique and field emission scanning electron microscopy. The obtained results showed that the particle size of the chitosan/extract biomaterials prepared by microemulsion method and solution method with STPP ranged from 68.06 nm to 1484 nm, with an average particle size of 304.9–1019 nm. The microemulsion method produced biomaterials with much smaller average particle size than the solution method using cross-linkers. The hemostatic ability of the biomaterials was better than that of the control sample based on the time of blood clotting formation and glomerular aggregation ability. The sample with the ratio of E. prostrata L. extract: chitosan of 1:30 had the lowest hemostasis time (6 min 46 s) and its glomerular aggregation rate after 5 min was 13.05%. This indicated that the biomaterials based on chitosan and E. prostrata L. extract are promising for application in biomedicine as hemostatic materials.

Enhanced sun protection factor of octocrylene with green tea and bhringraj extracts

Objectives The aim of this study of sun protection factor boosting effect sunscreen compositions with herbal extract and combination with octocrylene. Materials and Methods The standardized herbal extracts procured and studied their sun protection Factors. Camellia Sinensis (Green tea) leaf Extract, Eclipta prostrata (bhringraj) leaf extract are selected for the development of the herbal sunscreen composition along with octocrylene as synthetic sunscreen. The developed sunscreen composition contains defined concentration of herbal extracts and octocrylene were evaluated for their different physicochemical properties and stability. Sun protection factor boosting effect of herbal extracts is studied combination with octocrylene. Results Invitro SPF studied for herbal extract individually and combination with octocrylene. The results of Invitro SPF study revealed the presence of green tea extract and, Eclipta prostrata (bhringraj) leaf extract shows excellent sun protection factor (SPF) boosting value of sunscreen composition containing 5% concentration of herbal extract and octocrylene. The sunscreen formulation containing 5% herbal extracts are stable for 12 weeks in an oven (45 °C). Conclusion The findings of this in-vitro SPF study revealed a sun protection boosting capacity of green tea extract and bhringraj extract confirmed. Summary The over exposure of human skin to Ultra-Violet Radiation (UVR) can trigger photodamage, UV burn, pigmentation, erythema, and enhance the chance of dermal carcinoma. UVR causes DNA damage, which leads to dermal cancer. Daily sunscreens protect the skin from the adverse effects of sun rays, especially UVB (290-320 nm) and UVA (320-400 nm). The ozone layer filters UVC (200-290 nm) radiation when it enters into atmosphere of the earth. UVB causes sunburn, photo damage and cause mutagenic changes in nucleic acids. UVA increases ROS (Reactive Oxygen Species) accumulation. ROS is responsible for cell repair which leads to carcinogenesis, and the cause of photodamage. Herbal extracts contain polyphenols, and flavonoids act as a natural sunscreen that will filter Ultra-Violet (UVB) light and contain antioxidant characteristics to modulate the photo-oxidative damage that results from UV-induced Reactive Oxygen Species production. The UVR protection of most herbal extracts are required to attain the higher UV protection in the sun care products. The approaches for preparing sun care products with higher Sun Protection Factor (SPF) possible through Oil in water formulation with herbal extract combinations of octocrylene. The developed sunscreen composition containing different concentration of herbal extracts were evaluated for their other physicochemical properties and stability. Invitro SPF was studied for Camellia Sinensis (Green tea) Leaf Extract and Eclipta prostrata (bhringraj) leaf extract individually and in combination with octocrylene. The results of the Invitro SPF study revealed the Camellia Sinensis (Green tea) Leaf Extract with octocrylene shows better Sun protection factor than Eclipta prostrata (bhringraj) leaf extract with octocrylene. The Sun Protection Factor (SPF) enhancement value of sunscreen compositions was compared to both sunscreen macroemulsion and nanoemulsion.

Clinical evaluation of capsule BP Norm in the management of essential hypertension: A preliminary open-label parallel group study

Abstract Background: Since hypertension is so common and a major risk factor for cardiovascular illnesses and other complications, it is both a significant public health issue and an important topic of research. Hence, a clinical trial was planned. Aims: To evaluate the role of herbal preparation capsule BP Norm containing dry extract of Bhringraja (Eclipta alba (L) Hassk.), Haritaki (Terminalia chebula Retz.), Ashwagandha (Withania somnifera (L.) Dunal), Shankhpushpi (Convolvulus pluricaulis), and Sarpagandha (Rauwolfia serpentina [L.] Benth. Ex Kurzc) in newly diagnosed cases and in combination with allopathic antihypertensive drugs in the uncontrolled cases of essential hypertension. Materials and methods: A total of 103 patients meeting the seventh report of the Joint National Committee on the Prevention, Detection, Evaluation, and Treatment of High Blood Pressure criteria of essential hypertension (EHT) of age group 20–65 years of either sex participated in the study. Newly diagnosed cases who were not taking any antihypertensives were administered capsule BP Norm (250 mg), two capsules twice daily for 1 month, whereas uncontrolled cases of essential hypertension (systolic blood pressure [SBP] >140 mmHg and <200 mmHg and/or diastolic blood pressure [DBP] >90 mmHg and <120 mmHg) were administered 1 capsule of BP Norm (250 mg) twice daily with water in addition to continuing allopathic antihypertensive drugs for 1 month. The assessment was made through various variables such as SBP, DBP, chief complaints, associated complaints, Hamilton anxiety rating scale (HARS), Hamilton rating scale for depression (HRSD), lipid profile, and serum creatinine. Results: Capsule BP Norm produced highly significant reduction in both the SBP (16.82%, P < 0.001) and DBP (13.71%, P < 0.001) of newly diagnosed patients as well as in the SBP (18.49%, P < 0.001) and DBP (13.95%, P < 0.001) of uncontrolled cases (EHT). Capsule BP Norm provided significant relief in clinical features of EHT, HARS, and HRSD in both groups. Conclusion: Capsule BP Norm has promising antihypertensive effects in newly diagnosed cases as well as synergistic effects when combined with allopathic antihypertensive drugs in uncontrolled cases of essential hypertension.

Karyomorphology of Seven Different Angiospermic Plants

The focus of this study is on the karyomorphological examination of seven angiospermic plants: Centaurea cyanus L. belonging to the Cynareae tribe; Coreopsis grandiflora Nutt. ex Chapm. and Eclipta prostrata (Linn.) Linn. from the Heliantheae tribe; Eupatorium adenophorum Spreng from the Eupatorie tribe; Gnaphalium affine D. Don. from the Inuleae tribe; Rhynchospermum verticillatum Reinw. from the Astereae tribe; and Tridax procumbens L within the Asteraceae family. Cytological analysis was used to assess the chromosome count and karyomorphology of all seven plants studied in central Nepal. The somatic chromosomal number found in this inquiry was 2n=24 for Centaurea cyanus, which has the karyotype formula M12+ m6+sm2+ st4. Similarly, 2n=26 was found for Coreopsis grandiflora, which has the karyotype formula M14+sm12 and 2n=22 for Eclipta prostrata which has the karyotype formula M14+sm6+st2. The karyotype formula for Eupatorium adenophorum is M4+m12+sm26+st8 and 2n=50, while for Gnaphalium affine, it is M10+sm4, with a 2n=14 value. The karyotype formula for Rhynchospermum verticillatum is M4+ m8+ sm6 with 2n = 18. For Tridax procumbens L, 2n = 26 whose karyotype formula is M18+m4+sm2+ st2.The examined taxa's total length varied from 0.3 µm to 2.6 µm. Int. J. Appl. Sci. Biotechnol. Vol 12(1): 8-18.

Pharmacognostical studies of Eclipta prostrata (Linn.) grown in Sri Lanka

Eclipta prostrata Linn. is an annual herbaceous plant used in traditional medicine, commonly known as “Trailing Eclipta” or False Daisy in English, ‘Keekirindiya’ in Sinhala and ‘Kayyantakara’ in Tamil. The aim of this study was to conduct a detailed pharmacognostical evaluation of E. prostrata found in Sri Lanka. Anatomical, physicochemical, phytochemical studies, and quantification of phyto-constituents were performed as per WHO guidelines. Whole plant was sequentially extracted into solvents with different polarities. Phytochemical screening and Thin Layer Chromatography (TLC) fingerprinting were carried out. Anatomical study and powder microscopy revealed useful diagnostic features. Physicochemical parameters such as moisture content, ash values (total, acid insoluble, water soluble) and extractable matter in water and ethanol were evaluated. Phytochemical screening and TLC fingerprinting revealed the presence of different types of phyto-constituents. Alkaloid, tannin, saponin, total flavonoid and total polyphenol contents were quantified. In conclusion, pharmacognostical study aids in establishing the standardization parameters of E.prostrata found in Sri Lanka.

An Ecological Weed Control Strategy in Paddy Fields: Light Interception from Duckweed Mulching

Rice–duckweed coculturing as an advanced technique has proven effective for weed control. However, the complex environmental interactions underlying its effectiveness remain unclear. In this study, a controlled pot experiment was conducted to isolate the shading effect of duckweeds (Landoltia punctata and Spirodela polyrhiza) from their intricate environmental interactions with paddy weeds (Cyperus difformis, Eclipta prostrata, and Ammannia auriculata). The results revealed that as the duckweeds grew, there were notable decreases in total weed density (52.41–77.01%) and biomass (42.33–99.23%) while rice biomass remained unaffected. Duckweed covering allowed less than 8.64% of light to pass through and led to decreases in environmental temperature, pH, and dissolved oxygen concentration in the floodwater, but had little impact on inorganic nitrogen levels. Through screening with random forest analysis and structural equation modelling, it was found that weed density and biomass were predominantly influenced by the reduced light transmittance (54.1% and 77.2%, p ≤ 0.001) resulting from duckweed covering while the decline in dissolved oxygen content partly influenced weed density (18%). In conclusion, this study’s results bring a different perspective, confirming the sunlight-shading effect of duckweed as a critical mechanism significantly inhibiting the germination and growth of weeds. These findings provide a scientific basis for the improved application of ecological weed control methods in paddy fields.

Investigation of the Cognitive Activity Between the Leaf Extracts of Eclipta alba and Ziziphus jujuba in Diabetic Animal Models.

Diabetes mellitus (DM), a prevalent metabolic disorder, is associated with widespread damage to bodily systems, notably causing significant dysfunction within the peripheral and central nervous systems (CNS). The primary objective of this study is to explore the extent of DM's impact on cognitive and behavioral functions and to evaluate the therapeutic potential of ethanol leaf extracts from Ziziphus jujuba (ZJ) and Eclipta alba (EA) in mitigating these adverse effects. Utilizing an established animal model, we aimed to determine the effectiveness of these plant extracts in ameliorating the cognitive impairments commonly seen in diabetic states. In our experimental framework, we allocated Wistar rats (n=6 per group) into eight different groups, inducing DM through alloxan administration. The intervention groups were treated orally with either the standard antidiabetic drug glibenclamide or varying doses of ZJ and EA extracts over periods of seven and 21 days. Throughout the study, we carefully tracked fluctuations in blood glucose levels, noting considerable decreases, particularly following the 21-day treatment interval. Post-treatment, the rats' cognitive functions were assessed using the Morris water maze (MWM) test. This evaluation revealed significant cognitive enhancement in the diabetic rats administered with ZJ and EA extracts, with these groups displaying reduced latency in finding the submerged platform, indicative of improved learning and memory. These observations were statistically significant (p<0.01). The findings underscore the hypoglycemic effects of ZJ and EA extracts and suggest their viability as cognitive enhancers in the context of DM. The protective effects of these extracts against cognitive decline caused by DM are clear. They add important new information to the research on natural phytochemicals for managing chronic diseases. This study opens new avenues for the application of these substances in treating neurocognitive disorders associated with DM.

A Review on Pharmacological Activities of Eclipta alba (Keekirindiya-Bhringarāja)

This review article contributes to clarifying the pharmacological properties of Eclipta alba (Bhringarāja) which is traditionally used in medicaments in so many countries such as Sri Lanka, India, China, Brazil, Thailand, Indonesia, Philippines, Nepal, Malaysia, Myanmar, Japan, Korea, Hong Kong, Pakistan, etc. Pharmaceutical products containing Eclipta alba have been used for different kinds of diseases and Bhringarāja had been mentioned in different types of prescriptions; especially in Samhitha Grantha. The main objective of this literature review is a discussion about pharmacological activities of Eclipta alba (Bhringarāja) and to provide an integrated, synthesized overview of the current state of knowledge on Eclipta alba (Bhringarāja) with Ayurvedic and traditional medical knowledge. The systemic literature review was carried out to gather authentic information on Eclipta alba (Bhringarāja). Relevant data for this study were gathered from Ayurveda textbooks, research papers, peer-reviewed indexed scientific journals, and authentic websites. Eclipta alba (Bhringarāja) is a small branched perennial herb, found in sandy and clay soils (waterlogged areas). Research article shows that Eclipta alba (Bhringarāja) has numerous medicinal properties such as Hepatoprotective, Hair growthpromoting, Antidiabetic, Analgesic, Anti-Inflammatory, Neuropharmacological, Antioxidant, Antimicrobial, Cardiovascular, Immunomodulatory, Anti-epilepsy, Anti-venom, Anticancer, Antiulcer, Anthelmintic and Anti- hyperlipedic properties. The reason for these properties is stated to be its major chemical compounds such as wedelolactone, dimethyl wedeloctone, ecliptal, etc. I found through this research that most of the therapeutic actions such as Śōthara, Vedanāsthāpana, Vraṇaśodhana, Vraṇarōpaṇa, Savarṇakaraṇa, Chakshushya, Keśavardhana, Keśaranjana, etc. have been mentioned in Samhitha Grantha and Ayurveda pharmacopeia. Those actions have been confirmed by modern laboratory tests, clinical trials, and animal trials. &#x0D; Keywords: Eclipta alba, Bhringarāja, Ayurveda, Pharmacological activities.

Biochar-modified constructed wetlands using Eclipta alba as a plant for sustainable rural wastewater treatment.

Constructed wetlands (CWs) provide a low-cost, effective solution for domestic wastewater treatment in developing nations compared to costly traditional wastewater systems. Biochar which is an organic material created by pyrolysis offers straightforward, affordable methods for treating wastewater and lowering carbon footprint by acting as a substrate in CWs. Batch mode biochar-amended subsurface flow (SSF) CWs planted with Eclipta alba (L) with a hydraulic retention time (HRT) of 3days were used for the treatment of rural domestic wastewater in the present investigation. Two control CWs, without plants (C1) and with plants (C2), and five different amendments of biochar 5% (B5), 10% (B10), 15% (B15), 20% (B20) and 25% (B25) in ratio with soil were set up to check the treatment efficiency of CWs. Removal efficiency (RE%) of the CWs for parameters namely chemical oxygen demand (COD), biochemical oxygen demand (BOD), phosphate (PO42-), sulphate (SO42-), nitrate (NO3-) and total Kjeldhal nitrogen (TKN) was determined using standard methods. Removal efficiency of 93%, 91%, 74% and 77% was observed for BOD, COD, nitrate and sulphate, respectively, in the B25 amendment at HRT 72h. The highest removal of TKN (67%) was also observed in the B25 amendment at HRT of 72h. No stable trend for the removal of phosphates was found during the study, and maximum removal was observed at HRT 48h; afterward, phosphate was slightly inclined with the increasing HRT. The findings of one-way ANOVA using Tukey's test show significant variations (p < 0.05) in the removal efficiencies of pollutants after 72h between two controls (C1 and C2) and various biochar amendments in CWs, indicating a significant role of the wetland plants and concentration of the biochar as substrate. Biochar shows a positive impact on the removal of organic pollutants and nitrates. Hence, biochar-amended CWs can be a sustainable way of treating rural domestic wastewater.

Ethanol extract of Eclipta prostrata induces multiple myeloma ferroptosis via Keap1/Nrf2/HO-1 axis

BackgroundMultiple myeloma (MM) is an incurable hematological malignancy with limited therapeutic efficacy. Eclipta prostrata is a traditional Chinese medicinal plant reported to possess antitumor properties. However, the effects of E. prostrata in MM have not been explored. PurposeThe aim of this study was to define the mechanism of the ethanol extract of E. prostrata (EEEP) in treating MM and identify its major components. MethodsThe pro-ferroptotic effects of EEEP on cell death, cell proliferation, iron accumulation, lipid peroxidation, and mitochondrial morphology were determined in RPMI-8226 and U266 cells. The expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), kelch-like ECH-associated protein 1 (Keap1), heme oxygenase-1 (HO-1), glutathione peroxidase 4 (GPX4), and 4-hydroxynonenal (4HNE) were detected using western blotting during EEEP-mediated ferroptosis regulation. The RPMI-8226 and U266 xenograft mouse models were used to explore the in vivo anticancer effects of EEEP. Finally, high performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry system (UPLC-Q/TOF-MS) were used to identify the major constituents of EEEP. ResultsEEEP inhibited MM cell growth and induced cell death in vitro and in vivo. By promoting malondialdehyde and Fe2+ accumulation, lipid peroxidation, and GSH suppression, EEEP triggers ferroptosis in MM. Mechanistically, EEEP regulates the Keap1/Nrf2/HO-1 axis and stimulates ferroptosis. EEEP-induced lipid peroxidation and malondialdehyde accumulation were blocked by the Nrf2 activator NK-252. In addition, HPLC and UPLC-Q/TOF-MS analysis elucidated the main components of EEEP, including demethylwedelolactone, wedelolactone, chlorogenic acid and apigenin, which may play important roles in the anti-tumor function of EEEP. ConclusionIn summary, EEEP exerts its anti-MM function by inducing MM cell death and inhibiting tumor growth in mice. We also showed that EEEP can induce lipid peroxidation and accumulation of ferrous irons in MM cells both in vivo and in vitro, leading to ferroptosis. In addition, this anti-tumor function may be achieved by the EEEP activation of Keap1/Nrf2/HO-1 axis. This is the first study to reveal that EEEP exerts anti-MM activity through the Keap1/Nrf2/HO-1-dependent ferroptosis regulatory axis, making it a promising candidate for MM treatment.

A Comprehensive Review of Bhringraj (Eclipta alba L.) from Ayurveda Perspective

Background: Eclipta alba L. is commonly known as Bhringraj, a plant well known in Ayurveda for its varied medicinal uses. Plant extracts of Bhringraj have been used for a variety of cures, among which the commonly known properties of its extract are anti-hepatotoxic, anti-diabetic, anti-inflammatory, and hair rejuvenator. The present review of Bhringraj aims to explore its literature in Ayurveda texts as well as its current research. Materials and Methods: Exploration of Ayurveda literature was done from Ayurveda classical texts as well as information from internet research databases was collected and presented in an organized manner. Result: Bhringraj has been widely described in various samhitas and nighantus with various clinical indications such as Kasa, Krimi, Swasa, Kushta, Dantya, Keshya, Pandu, and Shiroroga. Conclusion: Bhringraj holds a wide potential as per the references obtained in the present review and more studies should be conducted to tap its potential in clinical trials for human benefit.

Eclipta thermalis, a previously common weed, threatened by the expansion of the exotic congener E. alba in Japanese rice paddies

AbstractRice paddies are wetland ecosystems recognized as important habitats for many organisms; however, the hybridization‐related extinction risk of native plant species has not been investigated in this system so far. Eclipta L. (Compositae) is a common paddy weed in Japan; however, its genetic composition might be altered due to the hybridization between the native E. thermalis and the closely related exotic E. alba. We examined Eclipta's genetic composition using 12 microsatellite markers (612 samples collected from 109 populations) and found (i) widespread geographical distribution of E. alba in Japan, (ii) hybridization with E. thermalis, and a large number of later‐generation hybrids, and (iii) widely varying situations among regions and populations. Eclipta alba appears to have invaded an open niche in northern Japan but has not yet reached southern Japan. Both E. alba and E. thermalis were found in central Japan; however, the latter had become rare due to hybridization‐mediated processes such as competition, and demographic and genetic swamping. Notably, endogenous and exogenous selection plays an important role in the invasion of E. alba, but to varying degrees among different areas. In summary, considering the genetic variability in E. thermalis, the genetic cluster of mainland Japan is in a highly critical situation due to the invasion of E. alba.

Keanekaragaman Tumbuhan Bawah di Kawasan Universitas Negeri Jakarta

Understory plants are a group of plants that form a layer near the surface of the soil. One of the functions of undergrowth is as a ground cover which contributes to increasing the organic matter content in the soil. This research was conducted in November 2023 in the garden behind the KH. Hasyim Asy'ari Building, Jakarta State University. The aim of this research is to determine the diversity of understory plants found in the Jakarta State University environment. The research method used was a survey using a sampling technique with a quadratic method. There are 4 types of undergrowth in the garden behind the KH. Hasyim Asy'ari Building, namely Cyanthillium cineroum, Pilosela officinarum, Eclipta prostrata, and Cynodon dactylon. The results of the calculation of the Shannon-Wiener diversity index based on its classification show that the garden behind the KH. Hasyim Asy'ari Building, Jakarta State University has a low level of understory plant diversity.

Chemical Constituents &amp; Therapeutic Uses of Eclipta Prostrata Linn

Eclipta prostrata Linn, commonly referred to as "False Daisy" or "Bhringraj," is a medicinal plant extensively utilized in traditional medical systems, particularly in Ayurveda and traditional Chinese medicine. This review paper aims to consolidate and assess existing literature concerning the chemical constituents and therapeutic benefits of Eclipta prostrata. The plant's recognition for harboring a diverse array of bioactive compounds and its potential in addressing various health conditions adds significance to this review. By shedding light on the pharmacological properties and therapeutic applications of Eclipta prostrata, this review paper paves the way for further research and the development of its therapeutic potential.

Psychoneuropharmacological Properties of Eclipta Alba (LINN) in Mice

Eclipta alba (Linn) (EA) has been found useful ethnomedicinally in the treatment of many medical conditions especially mental disorders.&#x0D; Aims of the Study: This study aimed to evaluate the acute toxicity profile, behavioural activity, antipsychotic and mechanism of action of the Aqueous extract of Eclipta alba (AEA).&#x0D; Methodology: Using Lorke’s method the LD50 of AEA was 1,264 mg/kg (i.p) and 3,807 mg/kg (oral) respectively was obtained, which shows that AEA was moderately toxic. The AEA was analysed using standard procedure: novelty-induced behaviour (NIB), through intraperitoneal route shows that AEA (p &lt; 0.0001) exhibits depressant effects on the central nervous system (CNS), while the antipsychotic models (catalepsy, amphetamine-induced hyperlocomotion, swimming-induced grooming and apomorphine-induced climbing tests), (p &lt; 0.0001) reveals that it possesses antipsychotic properties.&#x0D; Conclusion: This study shows that the mechanism of action of AEA (antagonist model: haloperidol, atropine and cyproheptadine) (p &lt; 0.0001) probably mediated via histaminergic, serotonergic and dopamine pathways, which provides scientific support for the ethno-medicinal use of the plant in the treatment of mental illness.

Zinc oxide nanoparticles from leaf extract of Eclipta prostrata: Biosynthesis and characterization towards potential agent against film forming bacteria in metal working fluids

Industrially important metal working fluids are reported to be vulnerable to microbial growth, leading to loss of its working efficiency. The present study is dedicated towards the green synthesis of Zinc Oxide (ZnO) nanoparticles using Eclipta prostrata leaf extract and investigated for antimicrobial activity. Characterization studies using Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FT-IR) displayed the presence of agglomerated rod shaped ZnO nanoparticles with characteristic peak between 700 and 500 cm−1, respectively. Antimicrobial testing against gram-positive and gram-negative bacteria, as well as fungi, showed the ZnO nanoparticles' effectiveness. Against gram-positive bacteria, inhibition ranged from 0.48 to 1.51 at concentrations of 25 μl to 100 μl. Against gram-negative bacteria, inhibition ranged from 0.82 to 2.02, and against fungi, inhibition ranged from 1.20 to 2.34, all at the highest concentration tested. The results of the study indicate that the ZnO nanoparticles were effective against both fungi and bacteria, more effectively against gram negative bacteria. The results suggest that the green synthesized ZnO nanoparticles from Eclipta prostrata can be exploited to reduce the load of microbial contamination in metal working fluids.

Herbomineral form of Eclipta alba (HMEA) improves age-related dementia by increasing memory performance in animal models: An in vivo approach to investigate the multitherapeutic potential of antioxidant-based Ayurvedic drugs

Dementia is a consequential event due to neurodegeneration, which is a prevalent aspect of aging and other diseases associated with neuropathological conditions. The main contributor to dementia is the depletion of neurons in cognitive centers that results in cognitive decline. Cellular damage and apoptosis can occur due to excessive oxidative stress and reduced antioxidant levels. Epidemiological research provides valuable insights into dementia, but current treatments primarily focus on alleviating symptoms rather than addressing the root cause. Both modern and traditional medicines recognize the importance of maintaining cellular antioxidant levels and reduction in amyloid beta aggregation, often relying on natural sources for support. The identification of herbal plants with abundant pharmacologically active secondary metabolites shows promise in the treatment of dementia. Eclipta alba is an ancient traditional herb that elicits nootropic effects. This study reports that the herbomineral form of E. alba (HMEA) constitutes pharmacologically active metabolites and enhances memory and learning in 9- to 12-month-old albino wistar rats. Interestingly, HMEA enhanced learning and memory in adult rats with no significant toxicity, and found to stabilize lipid profile levels in animal models. Histopathological studies of animals showed that HMEA enhanced overall cytoarchitecture of granular cell layer in the hippocampus. It depicts improved cellular morphology, arrangement, and density in the hippocampal region. Taken together, the current study proves HMEA to be an efficient neuroprotective agent in treating age-related dementia.

Biosynthesis of ZnO nanoparticles using aqueous extracts of Eclipta prostrata and Piper longum: characterization and assessment of their antioxidant, antibacterial, and photocatalytic properties.

Chemical syntheses of nanoparticles that release toxic substances into the environment rarely meet the strict requirements of green chemistry principles. Instead, green synthesis of nanoparticles using plant extracts brings a safe, rapid, and effective process, contributing to solving environmental pollution problems. Here, we report the green synthesis of multifunctional ZnO nanoparticles (ZnONPs) using aqueous extracts of E. prostrata leaves and P. longum fruits. The XRD results affirmed the existence of hexagonal crystalline ZnONPs with particle sizes of 17-30 nm. The optical analysis revealed bandgap energies of 3.10 eV and 3.16 eV for ZnONPs biosynthesized using E. prostrata and P. longum extracts, respectively. The synthesized ZnONPs showed potential antioxidant activity through DPPH and ABTS methods. Among the antibacterial outcomes against pathogenic bacterial strains (S. aureus, B. cereus, E. coli, and S. typhimurium), ZnONPs exhibited the highest zone of inhibition (18.5 mm) for S. aureus. Moreover, both ZnONPs biosynthesized using E. prostrata and P. longum extracts served as strong photocatalysts in the degradation of crystal violet with degradation efficiencies of 95.64% and 99.90%, respectively. Therefore, biosynthesized ZnONPs hold significant promise as antioxidants, antibacterial agents, and photocatalysts.