Odontocete species use echolocation signals (clicks) to forage and navigate. The aim of this study is to explore inter- and intra-specific variation in clicks among odontocete species in the Northwest Atlantic, Temperate Pacific, and Hawaii. Clicks were examined for seven species of odontocetes—bottlenose dolphins, common dolphins, striped dolphins, rough-toothed dolphins, pilot whales, Risso’s dolphins, and Cuvier’s beaked whales. Specially developed PAMGuard tools were used to automatically measure a suite of click parameters. Seven parameters were compared within and between species; duration, 10 dB bandwidth, 3 dB bandwidth, center frequency, peak frequency, sweep rate, and number of zero crossings. Significant differences in duration, center, and peak frequency were evident between species within study areas (Dunn’s test with Benjamini-Hochberg adjustment <0.05). Geographic variation in click parameters between the three study regions was also examined. Results showed statistically significant pair-wise differences between geographic regions for almost all echolocation click parameters and species (Dunn’s test with Benjamini-Hochberg adjustment <0.05). Results suggest species-specific differences in clicks among odontocetes and indicate that geographic variation exists for multiple species. The ecological significance of these findings will be discussed along with implications for classifier development.