An investigation entitled, “Effect of different rootstocks and scion compatibility study on Rosa sp.’’ was conducted to study the graft compatability and growth performance of grafted rose plants and the experiment was conducted in Vegetable Research Station, Tamil Nadu Agricultural University, Palur, Cuddalore district, Tamil Nadu during the year 2022. It was carried out using completely randomized design (CRD) with fifteen treatments (T1- Andhra red rose + Dog rose, T2-Andhra red rose + Button rose, T3-Andhra red rose + Ebb tide rose, T4- Andhra red rose +Fairy rose, T5- Andhra red rose + Edward rose, T6- Edward rose+ Dog rose, T7-Edward rose + Button rose, T8-Edward rose+ Ebb tide rose, T9-Edward rose + Fairy rose, T10-Edward rose+ Edward rose, T11-Damask rose + Dog rose, T12-Damask rose + Button rose, T13-Damask rose + Ebb tide rose, T14-Damask rose+ Fairy rose, T15-Damask rose+ Edward rose) and two replications. The results indicated a significant difference between the different treatment combination for graft union, success percentage, survival percentage, plant height (cm), leaf length (cm), leaf width (cm), days taken for leaf initiation. The experiment data revealed that the maximum graft success (90) and graft survival percentage (80), length of leaves (3.9 cm) and plant height (67.6) was recorded in the treatments T7 (Edward rose + Button rose). It can be concluded that the combination of rootstock as Edward rose and scion as Button rose is said to be most compatible and can be recommended for commercial cultivation.