Early Plant Research Articles

Relationships Among the Bryophytes and Vascular Plants: A Case Study in Deep-Time Reconstruction

Relationships of the three bryophyte lineages (liverworts, mosses, and hornworts) to vascular plants is a pivotal question in the study of the origin and evolution of land plants. In recent decades, this question has been subjected to intense phylogenetic analyses using morphological characters, multigene DNA sequences, and genome structural characters. A tentative consensus, reached ten years ago, suggested that bryophytes are a paraphyletic group, with liverworts being sister to all other land plants and hornworts being sister to vascular plants. However, several more recent nuclear phylogenomic studies have concluded that bryophytes represent a monophyletic group that is sister to vascular plants. A discussion is presented here on strengths and weaknesses of different types of characters (morphological traits, nucleotide sequences, and genome structural arrangements) and their suitability for resolving deep phylogenetic relationships. Moreover, several criteria for credible phylogenetic reconstruction are proposed. Strong statistical support for reconstructed relationships should be derived from high-quality, independent characters selected for suitability to the particular question being addressed. The relationships inferred in a study should be congruent with those from as many other lines of phylogenetic evidence as possible. Any incongruities should be explicable by well-understood biological mechanisms. It is concluded that the relationships of the three bryophyte lineages to vascular plants should currently be viewed as unresolved. This is a difficult phylogenetic problem; the land plants underwent a rapid radiation a long time ago. Yet, further exploration of analytical methods and careful choice of characters should lead to the eventual elucidation of diversification patterns among early land plants.

Smallholder farmers’ coping strategies to climate change and variability: Evidence from Ethiopia

Climate change poses significant challenges for smallholder farmers worldwide, particularly in regions like sub-Saharan Africa, including Ethiopia, requiring effective coping strategies for resilience. This study examines farmers’ exposure to climatic shocks and their adoption of coping strategies. Findings from a survey of 646 farm households and qualitative discussions revealed farmers’ high vulnerability to climate-related hazards. Smallholder farmers rely on ex-ante coping strategies, including early planting (48.5%), income diversification (41.3%), increasing savings (42 %), water management (39.2%), and sowing drought-resistant varieties (37%). Similarly, they resort to ex-post coping techniques, such as reducing expenses (46.1%), utilizing savings (41.6%), changes in consumption patterns (39.5%), seeking assistance from relatives (23.5%), borrowing (17.6%), selling assets (11.9%), and migration for employment (8.7%). Farmers also encounter constraints such as limited access to weather information, inadequate extension services, weak institutional support, insufficient skills, high poverty levels, and limited access to innovations. Factors like land size, climate training, self-efficacy, cost-effectiveness perception, and hazard consequence perception positively influence farmers’ adoption of proactive strategies against climate change, while being male, older, less educated, owning a farm, limited access to extension services, and market constraints hinder anticipatory measures. Additionally, household head being male, education level, farm experience, land ownership, and access to climate information positively impact the implementation of ex-post strategies, whereas age, agroecology, infrequent extension worker visits, and limited road access have negative effects. Enhancing access to climate information, institutional support, and design policy with an understanding of these multifaceted contexts could substantially improve smallholder farmers’ resilience to climate change.

Close-range imaging for green roofs: Feature detection, band matching, and image registration for mixed plant communities

Green roofs offer ecological benefits but harsh rooftop environment stressors like heat, water scarcity, and wind can limit optimal plant growth. Monitoring plant health is crucial for optimizing green roof performance and remote sensing provides a non-destructive approach, yet challenges persist in tight, urban settings. This allows for close-range ground monitoring as a viable solution in these emerging environments. Recent studies explored automatic image registration techniques, showing promise in homogeneous settings, but faced uncertainties in mixed species systems or early plant growth stages. Feature detection techniques such as Speeded up Robust Features (SURF) using fixed and moving image spaces have been proposed for alignment. This study aimed to assess feature detection, matching, and image registration techniques in mixed species plant communities on green roofs throughout the growing season. This study developed a sophisticated monitoring system using close-range multispectral sensors for aligning bands in mixed species plant communities, contributing to enhanced green roof management and sustainability. The results of the study showed no significant differences in band alignment accuracy between growth stages for mixed species bush bean/sedum and single species sedum modules, but significant differences were found in single species bush bean systems. Additionally, mixed species modules showed better accuracy compared to single species bush bean modules, indicating that heterogenous systems provide better alignment accuracy due to more diverse feature extractions.

Environmental conditions differently shape leaf, seed and seedling trait composition between and within elevations of tropical montane forests

The composition of plant functional traits varies in response to environmental conditions due to processes of community assembly and species sorting. However, there is a lack of understanding of how plant trait composition responds to environmental conditions at different spatial scales and across the plant life cycle. We investigated the trait composition of leaves (specific leaf area), seeds (seed mass) and seedlings (initial seedling height) across elevations and within elevations in relation to soil and light conditions in a tropical montane forest in southern Ecuador. We surveyed traits and communities of adult trees, seeds and seedlings on nine plots at three elevations (1000–3000 m a.s.l.) and calculated community‐weighted mean trait values to analyse trait variation across and within elevations. In addition, we measured two environmental factors (soil C/N ratio and canopy openness) to quantify local‐scale variation in environmental conditions within elevations. We found that community‐weighted means of specific leaf area, seed mass and initial seedling height decreased consistently with increasing elevation. Within elevations, mean trait values of trees, seeds and seedlings responded differently to local‐scale environmental conditions. Specific leaf area decreased with increasing soil C/N ratio, and initial seedling height decreased with increasing canopy openness. Seed mass was associated neither with soil nor with light conditions. Our findings show that broad‐scale and local‐scale processes differently shape the composition of leaf, seed and seedling traits in tropical forests, indicating a scale‐dependence in trait–environment associations. Furthermore, plant traits corresponding to different life stages were related differently to environmental conditions within elevations. Community assembly processes may therefore lead to differences in species sorting at early and late plant life stages.

Molecular insights into MpAGO1 and its regulatory miRNA, miR11707, in the high-temperature acclimation of Marchantia polymorpha.

As a model plant for bryophytes, Marchantia polymorpha offers insights into the role of RNA silencing in aiding early land plants navigate the challenges posed by high-temperature environments. Genomic analysis revealed unique ARGONAUTE1 ortholog gene (MpAGO1) in M. polymorpha that is regulated by two species-specific microRNAs (miRNAs), miR11707.1 and miR11707.2. Comparative studies of small RNA profiles from M. polymorpha cellular and MpAGO1 immunoprecipitation (MpAGO1-IP) profiles at various temperatures, along with analyses of Arabidopsis AGO1 (AtAGO1), revealed that MpAGO1 has a low-selectivity for a diverse range of small RNA species than AtAGO1. Protein structural comparisons revealed no discernible differences in the MID domains of MpAGO1 and AtAGO1, suggesting the complexity of miRNA species specificity and necessitating further exploration. Small RNA profiling and size exclusion chromatography have pinpointed a subset of M. polymorpha miRNAs, notably miR11707, that remain in free form within the cell at 22°C but are loaded into MpAGO1 at 28°C to engage in RNA silencing. Investigations into the mir11707 gene editing (mir11707ge) mutants provided evidence of the regulation of miR11707 in MpAGO1. Notably, while MpAGO1 mRNA expression decreases at 28°C, the stability of the MpAGO1 protein and its associated miRNAs is essential for enhancing the RISC activity, revealing the importance of RNA silencing in enabling M. polymorpha to survive thermal stress. This study advances our understanding of RNA silencing in bryophytes and provides groundbreaking insights into the evolutionary resilience of land plants to climatic adversities.

Continued decline in sublethal effects of Bt toxins on Helicoverpa zea (Lepidoptera: Noctuidae) in field corn.

The majority of field corn, Zea mays L., in the southeastern United States has been genetically engineered to express insecticidal toxins produced by the soil bacterium, Bacillus thuringiensis (Bt). Field corn is the most important mid-season host for corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), which has developed resistance to all Cry toxins in Bt corn. From 2020 to 2023, corn earworm pupae were collected from early- and late-planted pyramided hybrids expressing Bt toxins and non-Bt near-isolines in North and South Carolina (16 trials). A total of 5,856 pupae were collected across all trials, with 55 and 88% more pupae collected in later-planted trials relative to early plantings in North and South Carolina, respectively. Only 20 pupae were collected from hybrids expressing Cry1F + Cry1Ab + Vip3A20 across all trials. Averaged across trials, Cry1A.105 + Cry2Ab2 hybrids reduced pupal weight by 6 and 9% in North and South Carolina, respectively, relative to the non-Bt near-isoline. Cry1F + Cry1Ab hybrids reduced pupal weight on average by 3 and 8% in North and South Carolina, respectively, relative to the non-Bt near-isoline. The impact of the Bt toxins on pupal weight varied among trials. When combined with data from 2014 to 2019 from previous studies, a significant decline in the percent reduction in pupal weight over time was found in both states and hybrid families. This study demonstrates a continued decline in the sublethal impacts of Bt toxins on corn earworm, emphasizing the importance of insect resistance management practices.

Rarity and life-history strategies shape inbreeding and outbreeding effects on early plant fitness

Local abundance and regional distribution are two aspects of a species’ rarity. They are suggested to differentially alter genetic processes in plants: Locally rare species are hypothesized to suffer less from inbreeding and outbreeding than locally common species, thanks to genetic purging through long inbreeding histories and weaker local adaptations, respectively. Regionally rare species are hypothesized to be more susceptible to outbreeding, but less to inbreeding, compared to regionally common ones, due to small and declining range size. While this has major implications for plant conservation practices, we lack evidences and general understanding on how breeding effects on a plant’s early life fitness are related to its local and regional rarity. To investigate effects of inbreeding and outbreeding on plants’ early fitness, we performed self-, within- and between-population pollinations in eight pairs of closely related species differing in regional and local rarity. To avoid biases due to context dependency, we took species competitive ability, habitat resource-richness and resource-allocation strategy into account in the analyses. We then tested how inbreeding and outbreeding affected five fruit-, seed- and seedling-related traits. Inbreeding did not generally have more negative effects on early fitness of regionally rare and non-competitive species than on regionally common and competitive ones. Outbreeding was generally beneficial to early fitness of plant species across the gradients of regional rarity, competitive ability and habitat resource-richness. Our results show that outbreeding may be beneficial to the early fitness of plant species, including rare and non-competitive ones and may be considered for conservation strategies.

On Farm Performance of Finger Millet Varieties in Dera District of South Gondar Zone

Finger millet is an important food security crop and beneficial for human health. This study aimed to evaluate the on farm performance of the finger millet varieties in Dera district. The study took place in Korata Kebele and assessed grain yield and farmer feedback. The performance differences between the Necho variety (the demonstrated variety) and the farmers' variety were evaluated. Recent 10 years' grain yield data from the Dera district agriculture office was used to analyze the growth rates of area coverage, production, and productivity in the district using the compounded annual growth rate (CAGR). Furthermore, the performance of the Necho variety was compared to its potential and farmer variety using the technology gap, extension gap, and technology index. From 2014 to 2018, the annual growth rate of area coverage and productivity in Dera district declined by 1.6% and 1.7% per year, respectively. Moreover, from 2019 to 2023, the average annual growth rate of area coverage, production, and productivity decreased by 0.75%, 2.6%, and 3.4% per year, respectively. The technology index for the Necho variety in the production seasons of 2021, 2022, and 2023 was -4%, 16%, and 36%, respectively. The higher value in the 2023 season indicated a decline in the performance of the Necho variety compared to the farmer variety. This decline was attributed to the occurrence of blast disease. To address this issue, it is recommended to practice early planting and use recommended fungicides (Natura 250EW and Tilt 250EC). The district agriculture office must ensure easy accessibility of these fungicides in the market for farmers to utilize.

Early Paleogene plant biomes of the Pacific side of Eurasia

Major plant biome changes in East Asia during the early Paleogene are studied using the Integrated Plant Record vegetation analysis for the first time based on paleobotanical records. The paleobotanical record employed for the reconstruction comprises 110 paleofloras covering the early Paleocene to early Eocene, a time-span of ca. 17 Myr. Our data indicate the presence of more forested conditions over East Asia during the early Paleogene than at present. The early Paleogene vegetation cover in the study area fundamentally differed from modern and was much more homogeneous compared to the modern one. Mixed mesophytic zonal vegetation dominated on the Pacific side of Eurasia during the early Paleogene. Thermophilous mixed mesophytic forests were distributed even at very high latitudes, but at the same time, ecotone between the mixed mesophytic / broadleaved evergreen forests and broadleaved evergreen forest were confined to regions south of 50°N. The proportions of sclerophyllous plants are low in general, even in the mid-latitudes. The localities with open woodlands are all located south of ca. 45°N. The observed vegetation patterns and their changes throughout the early Paleogene in many cases can be correlated with climate patterns and the overall continental paleoclimate evolution as reconstructed from the paleobotanical record. The higher-than-present spatial homogeneity of early Paleogene vegetation coincides with shallow temperature gradients and a significantly more humid regional rainfall pattern over East Asia during the early Paleogene.

Non-invasive early monitoring plant health using terahertz spectroscopy

Non-invasive early monitoring plant health using terahertz spectroscopy

Soil amendments influence early plant survival and growth in reclamation of severely degraded lands by gold mining in the Peruvian Amazon

Gold mining has been causing the most severe impacts on the soils of the Peruvian Amazon. It has created challenges for their recovery. In this context, soil amendments could play a crucial role in plant establishment in post-mining soils. The study aimed to analyze the effects of two amendments on the early plant survival and growth of seven species in the reclamation of severely degraded lands by gold mining in the Southeastern Peruvian Amazon. The study was based on a completely randomized block design, including 2-amendment treatments (T1: sawdust + island guano manure and T2: T1 + organic soil + hydrogel) and a control. The plant survivorship, height growth, diameter growth, and biomass accumulation were measured. This study found that amendments may be effective at increasing survivorship and plant growth in degraded lands by gold mining in the Peruvian Amazon. The amendments increased the survival, diameter, height, and biomass of most plant species in the study. In general, survivorship and plant growth in T2 were high compared to T1. At the end of the experiment, the highest survivorship was for an Indigofera suffruticosa and Crotalaria pallida (>80%). The diameter growth was higher in T2 than in T1. The species growing fastest in diameter (>1.5 cm) were Crotalaria cajanifolia, C. pallida and Ochroma pyramidale. Soil amendments provided similar effects on height for most species except for I. suffruticosa. Therefore, C. pallida, I. suffruticosa, C. cajanifolia and O. pyramidale are key species to be considered in reforestation and/or restoration initiatives, due to its potential to acclimate and establish itself in severely degraded areas.

Optimizing pyrethrum planting dates to enhance productivity in a changing climate: A case of southern Highlands in Tanzania

Pyrethrum has excellent potential for growing and industrial production in Tanzania, offering an environmentally friendly alternative to synthetic pesticides and reducing pest and disease incidence without altering the natural balance. Planting date and seeding rate are critical for the productivity of pyrethrum; however, farmers in recent years have been experiencing climate change, resulting in unreliable planting periods. This study seeks to optimize the planting date for increased crop production. The study was conducted in the Umalila Highlands in the Mbeya region, with demonstration plots established in three villages (Iyunga Mapinduzi, Santilya, and Pashungu) and planted at monthly intervals from January to April. A randomized complete block design with three replications was used. The highest flower yield was achieved with early planting dates, particularly in January, with yields progressively decreasing from February to April. Sowing in January produced a yield of approx. 550 kg ha-1, significantly more than the April control with approx. 122 kg ha-1. The survival rate of Pyrethrum plants was highest at about 75% when planted in January and decreased to 72% in February, 67% in March and 57% in April. Pyrethrum planted in January had the most significant plant height, rosette size, and number of stems at all three sites, while April plantings had the worst performance and higher disease scores. Positive correlations were observed between yield and plant traits, such as height, rosette size, and number of stems, with negative correlations between yield and disease scores.

Development and Comparison of Visual LAMP and LAMP-TaqMan Assays for Colletotrichum siamense.

Strawberry anthracnose caused by Colletotrichum spp. has resulted in significant losses in strawberry production worldwide. Strawberry anthracnose occurs mainly at the seedling and early planting stages, and Colletotrichum siamense is the main pathogen in North China, where mycelia, anamorphic nuclei, and conidia produced in the soil are the main sources of infection. The detection of pathogens in soil is crucial for predicting the prevalence of anthracnose. In this study, a visualized loop-mediated isothermal amplification (LAMP) assay and a loop-mediated isothermal amplification method combined with a TaqMan probe (LAMP-TaqMan) assay were developed for the β-tubulin sequence of C. siamense. Both methods can detect Colletotrichum siamense genomic DNA at very low concentrations (104 copies/g) in soil, while both the visualized LAMP and LAMP-TaqMan assays exhibited a detection limit of 50 copies/μL, surpassing the sensitivity of conventional PCR and qPCR techniques, and both methods showed high specificity for C. siamense. The two methods were compared: LAMP-TaqMan exhibited enhanced specificity due to the incorporation of fluorescent molecular beacons, while visualized LAMP solely necessitated uncomplicated incubation at a constant temperature, with the results determined by the color change; therefore, the requirements for the instrument are relatively straightforward and user-friendly. In conclusion, both assays will help monitor populations of C. siamense in China and control strawberry anthracnose in the field.

A multi-omics dataset of the response to early plant polysaccharide ingestion in rabbits

The transition from a milk-based diet to exclusive solid feeding deeply modifies microbiota-host crosstalk. Specifically, early ingestion of plant polysaccharides would be one of the main nutritional components to drive host-microbiota-interaction. To capture the effects of polysaccharides early-life nutrition (starch vs rapidly fermentable fiber) on the holobiont development, we investigated on the one hand the gut bacteriome and metabolome and on the other hand the transcriptome of two host gut tissues. Rabbit model was used to study post-natal co-development of the gut microbiota and its host around weaning transition. The assessment of the microbial composition of the gut appendix together with the caecum was provided for the first time. Gene expression signatures were analyzed along the gut (ileum and caecum) through high-throughput qPCR. The data collected were completed by the analysis of animal growth changes and time-series assessment of blood biomarkers. Those accessible and reusable data could help highlight the gut development dynamics as well as biological adaptation processes at the onset of solid feeding.

Three-dimensional image analysis specifies the root distribution for drought avoidance in the early growth stage of rice.

Root system architecture (RSA) plays a key role in plant adaptation to drought because deep rooting enables better water uptake than shallow rooting under terminal drought. Understanding RSA during early plant development is essential for improving crop yields, as early drought can affect subsequent shoot growth. Herein, we demonstrate that root distribution in the topsoil significantly impacts shoot growth during the early stages of rice (Oryza sativa) development under drought, as assessed through three-dimensional (3D) image analysis. We used 109 F12 recombinant inbred lines (RILs) obtained from a cross between shallow-rooting lowland rice and deep-rooting upland rice, representing a population with diverse RSA. We applied a moderate drought during the early development of rice grown in a plant pot (25cm height) by stopping irrigation 14 days after sowing (DAS). Time-series RSA at 14, 21, and 28 DAS was visualized by X-ray computed tomography, and subsequently compared between drought and well-watered conditions. Following this analysis, we further investigated drought-avoidant RSA by testing 20 randomly selected RILs under drought conditions. We inferred the root location that most influences shoot growth using a hierarchical Bayes approach: the root segment depth, which positively impacted shoot growth, ranged between 1.7-3.4cm under drought conditions and between 0.0-1.7cm under well-watered conditions. Drought-avoidant RILs had a higher root density in the lower layers of the topsoil compared to the others. Fine classification of soil layers using 3D image analysis revealed that increasing root density in the lower layers of the topsoil, rather than in the subsoil, is advantageous for drought avoidance during the early growth stage of rice.

Planting time and seeding rate impact insect feeding, ear rots, and forage nutritive value in silage corn

AbstractPlanting date and seeding rate affect the microclimate within a crop field. They can influence lepidopteran insect infestation and fungal infections and are an important part of crop management strategy. Altering planting date and seeding rate can also influence yield and quality of silage corn (Zea mays L.). Field research was conducted to identify optimum planting time and seeding rate to minimize insect feeding, ear rot infections, and mycotoxin accumulation in silage corn without compromising yield and quality. Replicated field trials were conducted across multiple site‐years in Michigan with three planting dates (early: April 25–May 10; mid: May 11–25; and late: May 26–June 10) and/or four seeding rates (ranging from 69,160 to 113,620 seeds ha−1, in increments of 14,800 seeds ha−1). Mid‐planting yielded 12%–15% less forage than early and late plantings, partly due to greater insect feeding injury and fungal infections observed in the former. Neutral detergent fiber digestibility, starch, and crude protein concentration were greatest for early planting. Greater predicted milk per hectare and milk per megagram for early planting also indicated superior silage quality. Increasing seeding rate increased insect feeding and ear rot injury only when severity was >5% and >15%, respectively. The impact of increasing seeding rate on dry forage yield was specific to each site‐year. Overall, results showed that early planting of silage corn helps to escape insect and disease pressure and provides better yield and quality, while seeding rate response is variable and dependent on the field environment.

Adaptation strategies for cumin in Sabzevar, Iran: planting date and irrigation management

ABSTRACT The current study aimed to investigate the effect of planting date and irrigation on some characteristics of cumin cultivated in Iran. The experimental characteristics included three planting dates and five irrigation regimes. Irrigation at flowering + seed filling stage and full irrigation had the highest essential oil content (3.6%) and plant height (16.71 cm), respectively. On all planting dates, irrigation enhanced the total chlorophyll and carotenoids content, relative water content, umbel number plant−1, seed number umbel−1, 1,000-seed weight, and seed yield but reduced the proline content and water-use efficiency (WUE). The highest chlorophyll content (1.54 mg g−1 fresh weight (FW)), carotenoids content (0.35 mg g−1 FW), umbel number plant−1 (18.03), seed number umbel−1 (21.16), 1,000-seed weight (4.12 g), seed yield (1002.7 kg ha−1), and essential oil yield (22.13 kg ha−1) were obtained on 19 January and irrigation at the flowering + seed filling stages. The greatest amount of WUE (0.53 kg m−3) was measured on 9 January under no irrigation condition. Therefore, to produce cumin under similar climatic conditions, early planting of cumin in early January, along with two irrigations at the flowering and seed filling stages, is recommended.

Correction to: Picturing the flora of China: Early Qing dynasty plant paintings in Britain

Correction to: Picturing the flora of China: Early Qing dynasty plant paintings in Britain

Fire season matters for midstory hardwood control: Impacts of fire season and firing technique on plant communities

In the southeastern USA, lack of historical fire regimes often leads to hardwood encroachment into early successional plant communities and managed pine stands, reducing wildlife value and timber yields. Land managers lack information on how firing technique interacts with fire season to influence plant communities. We designed an experiment to quantify these interactions in east-central Mississippi with pairs of 4 m × 8 m plots randomly assigned a backing and heading fire in each of three seasons: February (Feb), May–June (May/Jun), and September–October (Sep/Oct). We used thermocouples to monitor fire temperature and tagged midstory trees to monitor response. We lit heading fires with an 18–25 kph wind generated by a backpack blower and backing fires into the ambient wind. Despite backing fires producing longer residence times than heading fires and raising temperature above the lethal threshold of 60 °C an average of 54 s longer, firing technique did not influence midstory response one growing season post-fire. Backing and heading fires produced similar maximum temperatures. For both firing techniques, May/Jun resulted in the highest midstory mortality rates which were 3-fold greater than Sep/Oct and 4-fold greater than Feb. Among all three fire seasons, trees with a 2.5 cm diameter at breast height (DBH) had approximately a 75% chance of top-kill which decreased to <20% as trees approached 6.5 cm DBH. We found no effects of fire season on fire temperature, rate of spread, flame height, or percent crown scorch. We found no significant interactions between fire season and firing technique. Understory analysis revealed Sep/Oct produced the greatest increase in forb coverage, May/Jun resulted in the most grass coverage, and Feb produced the most brambles (Rubus spp.). On sites with similar species, weather, and fuel conditions to ours, land managers should emphasize fire season over firing technique for midstory control and understory manipulation. Where midstory hardwood control with fire is a priority, fire return intervals should be frequent enough to prevent trees from exceeding 2.5 cm DBH to avoid trees escaping fire's reach. These data can help managers reduce midstory competition with crop trees and promote understory development for wildlife.

Planting date and tillage effects on yield and nutrient uptake of two sorghum cultivars grown in sub-humid and semi-arid regions in South Africa

IntroductionSorghum is emerging as a viable crop option to increase food production under changing climate due to its resilience to drought and marginal soils. Appropriate planting date, crop cultivars, type of tillage and rotations, under contrasting climatic conditions, could make significant contribution on the effective management of sorghum under changing climatic conditions. A short-term study was carried out to investigate the effects of planting date, crop cultivar, tillage type and rotations on the growth and primary nutrient uptake of sorghum in contrasting climatic regions.MethodsThe study was conducted in Free State - (FS) and KwaZulu Natal (KZN) Provinces of South Africa over two seasons (2020/21 and 2021/22). The two cultivars (Pan8816 and Macia) were sown in December (PD1) and in January (PD2) under conventional tillage (CT) and no-till (NT) and with two rotations (Sorghum-Cowpea-Sorghum and Sorghum-Sorghum-Sorghum) resulting in 2×2×2×2 factorial experiment.Results and discussionIn KZN, in both the first (2020/21) and second (2021/22) seasons, the effects of all the factors, except cultivar in the second season, were not significant on yield, and uptake of nitrogen (N), phosphorus (P) and potassium (K). In the second season, Pan8816 (4.40 t/ha) produced 3.3 times higher yield than Macia (1.32 t/ha), and took up higher N, K and P. In FS, the early planted sorghum (1.15 t/ha for season one; 3.39 t/ha for season two) had significantly higher yield than late planted sorghum (0.434 and 0.91 t/ha for seasons one and two, respectively). Furthermore, the early-planted crops took up higher N, K and P than when planted late, while Pan8816 took up 1.74 times more P than Macia. Plants grown under CT (2.61 t/ha) produced significantly higher yield than under NT (1.70 t/ha), with plants under CT taking up significantly more K than those grown on NT. These findings suggest that in the drier FS, early planting under conventional tillage, while in the wetter KZN, selecting the appropriate cultivar (PAN8816), are essential for sorghum grain yield, at least in the short-term.