The dynamic modulus of in-service asphalt pavements serves as a critical parameter for the computation of residual life and the design of overlays. However, its acquisition is currently limited to laboratory dynamic modulus testing using a limited number of core samples, necessitating a reassessment of its representativeness. To facilitate the prediction of dynamic modulus design parameters through Falling Weight Deflectometer (FWD) back-calculated modulus data, an integrated approach encompassing FWD testing, modulus back-calculation, core sample dynamic modulus testing, and asphalt DSR testing was employed to concurrently acquire dynamic modulus at identical locations under varying temperatures and frequencies. Dynamic modulus prediction models for in-service asphalt pavements were developed utilizing fundamental model deduction and gene expression programming (GEP) techniques. The findings indicate that GEP exhibits superior efficacy in the development of dynamic modulus prediction models. The dynamic modulus prediction model developed can enhance both the precision and representativeness of asphalt pavement’s dynamic modulus design parameters, as well as refine the accuracy of residual life estimations for in-service asphalt pavements. Concurrently, the modulus derived from FWD back-calculation can be transmuted into the dynamic modulus adhering to a uniform standard criterion, facilitating the identification of problematic segments within the asphalt structural layer. This is of paramount importance for the maintenance or reconstruction of in-service asphalt pavements.