In the present work, cotton fabrics were dyed using the acidified aqueous extract of Psuderanthimum reticulatum leaves with different mordants such as ferrous sulphate, stannous chloride, copper sulphate, potash alum and ferric alum by premordanting technique and various shades were obtained. The washing, perspiration and light fastness properties of the dyed fabrics were carried out by standard methods, ISO 105-C06 A2S-2010, ISO 105 E04-2013 and ISO 105 B02 Methods 3:2014, respectively. Cotton fabrics dyed with copper sulphate and ferric alum showed excellent to very good properties towards the entire fastness tests studied. The bioactive colourant present in the aqueous extract of Psuderanthimum reticulatum responsible for dyeing is anthocyanin pigment and it was identified by chemical test, UV & FTIR spectral and HPLC analysis.